##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938098_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 515847 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.49202767487259 33.0 31.0 34.0 31.0 34.0 2 32.711238022126714 34.0 31.0 34.0 31.0 34.0 3 32.656756751517406 34.0 31.0 34.0 31.0 34.0 4 36.18923634333436 37.0 35.0 37.0 35.0 37.0 5 36.16399048555095 37.0 35.0 37.0 35.0 37.0 6 36.22966693612641 37.0 37.0 37.0 35.0 37.0 7 36.19139589839623 37.0 37.0 37.0 35.0 37.0 8 36.19803352544456 37.0 37.0 37.0 35.0 37.0 9 37.90073413240748 39.0 38.0 39.0 35.0 39.0 10-11 37.878330202560065 39.0 38.0 39.0 35.0 39.0 12-13 37.82850922851155 39.0 38.0 39.0 35.0 39.0 14-15 39.101295539181194 40.0 38.5 41.0 36.0 41.0 16-17 39.03935469237972 40.0 38.0 41.0 36.0 41.0 18-19 39.0520212388557 40.0 38.5 41.0 35.5 41.0 20-21 39.0825535478543 40.0 39.0 41.0 35.0 41.0 22-23 39.07191182656873 40.0 39.0 41.0 35.0 41.0 24-25 39.02087925295679 40.0 38.5 41.0 35.0 41.0 26-27 38.84801985860148 40.0 38.0 41.0 35.0 41.0 28-29 38.749715516422505 40.0 38.0 41.0 35.0 41.0 30-31 38.490652267048176 40.0 38.0 41.0 34.5 41.0 32-33 38.24226369446754 40.0 37.5 41.0 34.0 41.0 34-35 38.02953588951763 40.0 37.0 41.0 34.0 41.0 36-37 37.91369630917694 40.0 37.0 41.0 34.0 41.0 38-39 37.82102542032812 40.0 37.0 41.0 33.0 41.0 40-41 37.687781454578584 40.0 37.0 41.0 33.0 41.0 42-43 37.46951033930604 40.0 37.0 41.0 33.0 41.0 44-45 37.167037900772904 40.0 37.0 41.0 32.5 41.0 46-47 36.726602073870744 40.0 36.0 41.0 31.0 41.0 48-49 36.321235754012335 40.0 36.0 41.0 30.0 41.0 50-51 36.003277134499186 40.0 35.5 41.0 29.5 41.0 52-53 35.57949353199689 39.0 35.0 41.0 27.5 41.0 54-55 35.32151296799245 39.0 35.0 41.0 26.5 41.0 56-57 35.065978865826494 39.0 35.0 40.0 26.0 41.0 58-59 34.72133500824857 38.5 35.0 40.0 24.5 41.0 60-61 34.32587375714117 38.0 34.0 40.0 22.5 41.0 62-63 33.903949233009016 38.0 34.0 40.0 21.0 41.0 64-65 33.48292032327415 37.5 33.5 40.0 18.5 41.0 66-67 32.95939009047256 37.0 33.0 39.5 10.5 41.0 68-69 32.4278061130529 36.0 32.5 39.0 7.5 40.5 70-71 31.887373581701553 36.0 31.5 39.0 2.0 40.0 72-73 31.442109772859006 35.0 31.0 38.0 2.0 40.0 74-75 30.919826033688285 35.0 31.0 37.5 2.0 39.5 76-77 29.613852557056646 33.5 29.5 36.0 2.0 38.5 78-79 30.264260526861648 34.5 30.5 36.0 2.0 39.0 80-81 30.088228680209443 35.0 31.0 36.0 2.0 38.5 82-83 29.67391687845427 34.0 30.0 36.0 2.0 37.0 84-85 29.206627158828102 34.0 29.5 35.0 2.0 37.0 86-87 28.731883678687673 34.0 29.0 35.0 2.0 36.0 88-89 28.209305278503123 34.0 29.0 35.0 2.0 36.0 90-91 27.776259239658273 34.0 27.5 35.0 2.0 35.5 92-93 27.270765362597825 33.0 26.5 35.0 2.0 35.0 94-95 26.696076549829698 33.0 25.5 35.0 2.0 35.0 96-97 26.06594687959802 33.0 24.5 35.0 2.0 35.0 98-99 25.26145737011168 32.5 20.5 34.5 2.0 35.0 100-101 22.033642727397854 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.02778542743298118 1101 2 -0.07992586407108604 1101 3 0.13523459189612907 1101 4 0.07946457564824527 1101 5 -0.0199782143556817 1101 6 0.03515314438461559 1101 7 -1.8094400312662628E-4 1101 8 0.02405043611454971 1101 9 -0.010899094147639232 1101 10-11 -0.05040355409033026 1101 12-13 -0.04369850987371393 1101 14-15 -0.12063004378688902 1101 16-17 0.06313707098063759 1101 18-19 0.06885238021504847 1101 20-21 0.04080396193759128 1101 22-23 -0.0736187688114569 1101 24-25 -0.14105276791293164 1101 26-27 0.0732424031141008 1101 28-29 -0.11806105290182245 1101 30-31 0.12632248598149687 1101 32-33 0.18291046318926618 1101 34-35 -0.0654590217416029 1101 36-37 -0.039010280869085534 1101 38-39 -0.14703866560882517 1101 40-41 0.0314688548546016 1101 42-43 -0.11541170532346001 1101 44-45 -0.039304066198738496 1101 46-47 0.18104945253809035 1101 48-49 -0.10931722705485214 1101 50-51 0.14893994615403727 1101 52-53 0.24659000311022083 1101 54-55 0.025833436869554305 1101 56-57 0.29269994084401674 1101 58-59 0.5147497035000086 1101 60-61 0.5751168452960229 1101 62-63 0.6252091780945861 1101 64-65 0.760336882218354 1101 66-67 0.6084017285319518 1101 68-69 0.8865452707894903 1101 70-71 0.8670227390039003 1101 72-73 0.7595123448085168 1101 74-75 0.659686684250989 1101 76-77 0.8983389346342214 1101 78-79 0.8255400429203732 1101 80-81 0.9341204165696944 1101 82-83 0.6708160555893521 1101 84-85 0.9613647841764497 1101 86-87 0.5719774479272708 1101 88-89 0.9914035254740483 1101 90-91 1.0767978548709678 1101 92-93 1.1225747675098035 1101 94-95 1.200085338052638 1101 96-97 1.5168809635498235 1101 98-99 1.575297103855906 1101 100-101 2.213750882799115 1102 1 0.11459590483666204 1102 2 0.09985076137765958 1102 3 0.18647112959058632 1102 4 0.14312632275634485 1102 5 0.03674296633837315 1102 6 0.009445126833760753 1102 7 0.10644051590912085 1102 8 0.08769223906627843 1102 9 0.17721418866049987 1102 10-11 0.3753642959296073 1102 12-13 0.2680984586145172 1102 14-15 0.3418549980958403 1102 16-17 0.2543117817903706 1102 18-19 0.20055361675274241 1102 20-21 0.3876627573105438 1102 22-23 0.3268698630194109 1102 24-25 0.26891931026815286 1102 26-27 0.3776799779046911 1102 28-29 0.2396772797667026 1102 30-31 0.22813740421045736 1102 32-33 0.2185706147648574 1102 34-35 0.1050734872332697 1102 36-37 0.06456969519793176 1102 38-39 0.3003486499077255 1102 40-41 0.1604676582052278 1102 42-43 0.06322810321264427 1102 44-45 0.10739517592332959 1102 46-47 -0.15363343537575247 1102 48-49 -0.015888826576187398 1102 50-51 0.1730723753522838 1102 52-53 -0.04622012852120605 1102 54-55 0.11792557887194732 1102 56-57 -0.04370811659514828 1102 58-59 0.11591643664719697 1102 60-61 0.13469004034987364 1102 62-63 0.149890071592786 1102 64-65 0.1523193313607507 1102 66-67 0.30527049598308764 1102 68-69 0.46753848977632373 1102 70-71 0.6165241350948492 1102 72-73 0.584053629212189 1102 74-75 0.5340484712473987 1102 76-77 0.6157102948256856 1102 78-79 0.5372672068613404 1102 80-81 0.5613342179259 1102 82-83 0.4410095139060708 1102 84-85 0.5023699696571029 1102 86-87 0.6638302600533201 1102 88-89 0.5550054401130637 1102 90-91 0.616496698109902 1102 92-93 0.8304128209601451 1102 94-95 0.41691820602232 1102 96-97 0.763709842688236 1102 98-99 1.1646568964366821 1102 100-101 2.095930779089503 1103 1 0.3681883393886167 1103 2 0.36734009363460274 1103 3 0.4661851187498556 1103 4 0.19752604597165657 1103 5 0.14609021579056503 1103 6 0.2520757346067981 1103 7 0.1939275416270263 1103 8 0.21257402894057265 1103 9 0.2245789678093857 1103 10-11 0.39131401337857596 1103 12-13 0.46396398738858835 1103 14-15 0.4474685374799918 1103 16-17 0.3490586391309378 1103 18-19 0.39158982941564346 1103 20-21 0.2218110677706946 1103 22-23 0.49316267815031267 1103 24-25 0.49327059616366853 1103 26-27 0.4066933558311163 1103 28-29 0.5462826309372701 1103 30-31 0.499746791824343 1103 32-33 0.7612906009256193 1103 34-35 0.612618753061895 1103 36-37 0.8581315681076234 1103 38-39 0.8882076050455439 1103 40-41 0.868004274512451 1103 42-43 0.8743888689836083 1103 44-45 1.1951215050664459 1103 46-47 1.3373595296801781 1103 48-49 1.4292386097056706 1103 50-51 1.394413627802983 1103 52-53 1.4338542573627748 1103 54-55 1.552261295628952 1103 56-57 1.2946467444358731 1103 58-59 1.3187765450545328 1103 60-61 1.5845314657910023 1103 62-63 1.2108259498075213 1103 64-65 1.1699960699145535 1103 66-67 0.9159814866416056 1103 68-69 1.1622795807692867 1103 70-71 1.0480055097921124 1103 72-73 0.7030854594509144 1103 74-75 0.5616894584461072 1103 76-77 0.5785490677469163 1103 78-79 0.6700056453344096 1103 80-81 0.5186603625458979 1103 82-83 0.7917764381362602 1103 84-85 1.0703523246334576 1103 86-87 1.2344017051000264 1103 88-89 1.1693261643663178 1103 90-91 0.882287840999993 1103 92-93 1.3891834356527966 1103 94-95 1.3862210545980354 1103 96-97 2.0738067173278374 1103 98-99 2.002383225579802 1103 100-101 2.338102962228703 1104 1 0.09603137757648028 1104 2 0.06817374274411492 1104 3 0.09345870723034011 1104 4 0.09726710950582884 1104 5 0.08900314577246604 1104 6 0.11781296672334207 1104 7 -0.012158517907309374 1104 8 0.0990103895148664 1104 9 0.20770073110356435 1104 10-11 0.13098023795858893 1104 12-13 0.05443386234122016 1104 14-15 0.08890468753683933 1104 16-17 0.25311267896083933 1104 18-19 0.2895546519494303 1104 20-21 0.15123246055416217 1104 22-23 0.1907069920739346 1104 24-25 0.16615015912943676 1104 26-27 0.37158957072733045 1104 28-29 0.2633919105465452 1104 30-31 0.2533703234651128 1104 32-33 0.2721506699753462 1104 34-35 0.4253029558323078 1104 36-37 0.49686783184388617 1104 38-39 0.15605948496639144 1104 40-41 0.27874750637636936 1104 42-43 0.1210179582851012 1104 44-45 0.10179648717246437 1104 46-47 0.37641314847518004 1104 48-49 0.2528223535480336 1104 50-51 0.5469940454696101 1104 52-53 0.7256656547776217 1104 54-55 0.722480444296373 1104 56-57 0.7883312208789661 1104 58-59 0.5827470094560354 1104 60-61 0.7209046711297162 1104 62-63 0.5198866209371644 1104 64-65 0.7445639828445323 1104 66-67 0.7676324697581052 1104 68-69 1.03018514264037 1104 70-71 1.1898074339216294 1104 72-73 1.1892123593709165 1104 74-75 1.0413034746980543 1104 76-77 0.8662106398912499 1104 78-79 0.8975329073444307 1104 80-81 1.1957976409762772 1104 82-83 1.2066668638025497 1104 84-85 1.1758689344604143 1104 86-87 1.0388388866923783 1104 88-89 1.1777021274836628 1104 90-91 1.1601129852044458 1104 92-93 1.5118914268952715 1104 94-95 1.484024831281122 1104 96-97 1.8896328930678088 1104 98-99 2.3108439219715358 1104 100-101 3.0200163553489894 1105 1 0.25952344106854497 1105 2 0.13932220212787172 1105 3 0.3236407800594705 1105 4 0.1486676697299174 1105 5 0.17600127835883228 1105 6 0.1546710432873013 1105 7 0.21692644941295214 1105 8 0.14238107112084464 1105 9 0.26519372830244237 1105 10-11 0.18465670854683225 1105 12-13 0.17530220968016152 1105 14-15 0.20347051386737292 1105 16-17 0.3496229497358101 1105 18-19 0.3041554922855667 1105 20-21 0.2367249908996314 1105 22-23 0.20608981186852304 1105 24-25 0.2804708874207549 1105 26-27 0.2021684689532819 1105 28-29 0.3719236565782964 1105 30-31 0.2483633206639908 1105 32-33 0.22865160368215953 1105 34-35 0.3528356355708695 1105 36-37 0.6612235741408057 1105 38-39 0.6911201759094325 1105 40-41 0.6019851347610583 1105 42-43 0.6771454092654992 1105 44-45 0.6618921921211154 1105 46-47 0.02070586556201448 1105 48-49 0.11203103702143125 1105 50-51 0.2075566038262835 1105 52-53 0.2772879703705229 1105 54-55 0.5980406683860124 1105 56-57 0.5122808007109043 1105 58-59 0.35696502999758195 1105 60-61 0.4914462210830379 1105 62-63 0.42105375445724036 1105 64-65 0.44931421627124024 1105 66-67 0.544464869384619 1105 68-69 0.5041113798019055 1105 70-71 0.7819759680019231 1105 72-73 0.59140657038866 1105 74-75 0.4700032879566898 1105 76-77 0.4391331422255149 1105 78-79 0.5939381361026008 1105 80-81 0.4907672955047566 1105 82-83 0.7365338105812604 1105 84-85 1.1879837803987918 1105 86-87 1.226852425441212 1105 88-89 1.3192637521335229 1105 90-91 1.2423492130289127 1105 92-93 1.5634903998177734 1105 94-95 1.334970209432381 1105 96-97 1.536003107820374 1105 98-99 1.8887617557717213 1105 100-101 2.034698895031532 1106 1 0.10020687111367721 1106 2 0.05228537579191794 1106 3 0.12637525876390754 1106 4 -0.001125632503935492 1106 5 0.04928606616176978 1106 6 -0.026813388009394146 1106 7 -0.0015739488121013778 1106 8 -0.10229598732342282 1106 9 -0.06206762111160202 1106 10-11 -0.09584922690278574 1106 12-13 -0.05699194589309542 1106 14-15 -0.02100136385857354 1106 16-17 -0.07833055274835488 1106 18-19 -0.22001866480473353 1106 20-21 -0.14186377536122308 1106 22-23 0.14412030452979963 1106 24-25 -0.07774203024147397 1106 26-27 -0.02404611746155183 1106 28-29 -0.3000943962908167 1106 30-31 -0.33147701005243135 1106 32-33 -0.2789127760349075 1106 34-35 -0.18767523028417799 1106 36-37 -0.19424159064851665 1106 38-39 -0.3338011272346506 1106 40-41 -0.41239837492907583 1106 42-43 -0.5315852009669939 1106 44-45 -0.568437386518859 1106 46-47 -1.147712991342452 1106 48-49 -0.849191324263515 1106 50-51 -0.6886507766113894 1106 52-53 -0.5880816204058235 1106 54-55 -0.686621978103922 1106 56-57 -0.7222149705182872 1106 58-59 -0.7125252419332426 1106 60-61 -0.6101106492473036 1106 62-63 -0.2447045798242513 1106 64-65 -0.30943535705906555 1106 66-67 -0.44403780441216867 1106 68-69 -1.0087797340904672 1106 70-71 -0.9661959893690337 1106 72-73 -0.7256659065711766 1106 74-75 -0.30741355885940536 1106 76-77 -0.2849898145006975 1106 78-79 -0.3208208429242134 1106 80-81 -0.006282046475750036 1106 82-83 -0.26528095787931605 1106 84-85 0.08764244463818116 1106 86-87 -0.13055835318358433 1106 88-89 -0.17737409062447185 1106 90-91 -0.19700300754984212 1106 92-93 0.040454028596172975 1106 94-95 0.30938519786489493 1106 96-97 0.11156536367048986 1106 98-99 0.41870827983589365 1106 100-101 1.100882002704001 1107 1 0.18777202863916642 1107 2 0.1299170841484738 1107 3 0.09584638546547097 1107 4 0.10096632930674332 1107 5 0.18817586570789757 1107 6 0.13869674396305243 1107 7 0.09471530506821324 1107 8 0.09625278929964054 1107 9 0.12662055892169377 1107 10-11 0.20242562402262365 1107 12-13 0.07400609801515401 1107 14-15 0.25402440774130497 1107 16-17 0.1165610244007027 1107 18-19 0.16951496940974664 1107 20-21 0.13278680545595734 1107 22-23 0.19269111905806113 1107 24-25 0.1522444556050928 1107 26-27 -0.014138166695325083 1107 28-29 -0.11640026055918185 1107 30-31 -0.18446596924405867 1107 32-33 -0.11453550166844906 1107 34-35 -0.07528555640875112 1107 36-37 0.045731162486880805 1107 38-39 0.0750129420554444 1107 40-41 0.3594073126717703 1107 42-43 0.22000571723049234 1107 44-45 0.20381088050041285 1107 46-47 0.5155878861668626 1107 48-49 0.5011913339085439 1107 50-51 0.38831433064581944 1107 52-53 0.5095135159559945 1107 54-55 0.5999017518024274 1107 56-57 0.5476801581940052 1107 58-59 0.5099228232843913 1107 60-61 0.49424338514640453 1107 62-63 0.3615600134580106 1107 64-65 0.4948498316473362 1107 66-67 0.541324291119416 1107 68-69 0.4005443032537599 1107 70-71 0.5947542996735748 1107 72-73 0.44398771584119245 1107 74-75 0.7011265847330286 1107 76-77 0.8353389691890669 1107 78-79 0.5971562595101574 1107 80-81 0.6839433229778926 1107 82-83 0.8592933098611972 1107 84-85 0.9222033439304163 1107 86-87 1.2186714129232108 1107 88-89 1.3592161172603632 1107 90-91 1.3808891487772215 1107 92-93 1.273039530204393 1107 94-95 1.7508226521043682 1107 96-97 1.5617979455294702 1107 98-99 2.0920518853828867 1107 100-101 2.233663113487278 1108 1 -0.010689324888900842 1108 2 0.028913357739050127 1108 3 0.1252469544441155 1108 4 0.09220125337613894 1108 5 0.05831250072011329 1108 6 -0.01520797214866576 1108 7 0.0061629950950532475 1108 8 0.04438357424975692 1108 9 0.029002656128888304 1108 10-11 0.06670718831320244 1108 12-13 -0.08133108193435845 1108 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-3.128588775444559 2316 98-99 -3.582419274780033 2316 100-101 -4.074215833650637 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 3.0 4 4.0 5 23.0 6 34.0 7 61.0 8 161.0 9 298.0 10 550.0 11 934.0 12 1450.0 13 2023.0 14 2675.0 15 3626.0 16 4880.0 17 6802.0 18 6771.0 19 4549.0 20 3681.0 21 3795.0 22 4069.0 23 4335.0 24 4987.0 25 5556.0 26 5552.0 27 5712.0 28 6792.0 29 8329.0 30 10040.0 31 12370.0 32 15423.0 33 20153.0 34 27906.0 35 43774.0 36 65039.0 37 114346.0 38 109688.0 39 9455.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.10075274257677 21.347996595889867 15.683526316911797 21.867724344621564 2 15.284337218590297 22.37202304787164 40.49856920177744 21.84507053176062 3 18.446852275128585 26.3031268841469 31.852963357159613 23.397057483564907 4 12.147594150978875 20.26666821751411 37.330448757092704 30.255288874414312 5 12.109451482518974 38.67285715670405 35.546142736674774 13.6715486241022 6 28.53830690107726 38.400533491519774 18.203847264789754 14.857312342613216 7 24.969031515158566 35.02298162051926 21.800844048719874 18.2071428156023 8 23.709161825114812 36.11671677842461 21.271423503480683 18.902697892979894 9 24.45477913951063 17.51724351841441 21.85225823210803 36.175719109966934 10-11 22.799008233061354 28.190917074248762 28.649095565157882 20.360979127532 12-13 24.304687242535092 26.421206287910948 28.407163364330895 20.86694310522306 14-15 21.572384835038296 28.342900123486224 27.494392717220418 22.59032232425506 16-17 19.60988432616648 31.193551576339495 28.723633170300495 20.472930927193527 18-19 19.04440657791942 29.339998100211883 31.85789584896297 19.757699472905728 20-21 20.53118463420355 28.28280478513978 31.86206375146119 19.323946829195478 22-23 19.85530593373617 29.00133179024013 31.478810577554974 19.66455169846873 24-25 19.669239164781395 29.023575093875824 32.098262836804366 19.208922904538415 26-27 20.013592067351503 29.03060057160363 31.89530811917587 19.060499241868996 28-29 19.62446156411022 29.18186823199172 32.080504646735655 19.11316555716241 30-31 19.816825531601427 29.439155408483526 32.02596894040287 18.71805011951218 32-33 19.553943919725803 29.364405465345307 31.864928891577105 19.21672172335179 34-35 19.86631767728176 29.194125345354077 31.948427124230527 18.991129853133636 36-37 19.448133335142774 29.370430756882826 31.604186013148176 19.577249894826227 38-39 19.329338461195416 28.803215545682022 32.187643300122446 19.67980269300012 40-41 19.589673786588488 28.680177455132082 32.091515814292585 19.63863294398685 42-43 19.537365030062258 28.81420342288997 31.980192452735075 19.668239094312696 44-45 19.638910430399378 29.08889104303994 31.466944552152 19.805253974408686 46-47 19.799530085026696 28.574668791364733 31.022724188601845 20.603076935006726 48-49 19.694036888880703 28.552292203203862 31.199882135480223 20.55378877243521 50-51 19.732982841811623 28.783631580681867 30.951425519582358 20.531960057924152 52-53 19.733758265532224 28.979910709958574 30.994752319970843 20.29157870453836 54-55 19.824482840842343 28.80379259741745 30.55179151957848 20.819933042161725 56-57 20.060793219694986 29.107274056067013 30.05532648246476 20.776606241773237 58-59 19.640416635165078 29.445358798248318 30.287662814749332 20.62656175183727 60-61 19.88108877244609 29.656758690076707 29.67924597797409 20.782906559503108 62-63 19.495993967203454 29.94657330565071 29.755722142418193 20.801710584727644 64-65 19.690237609213586 29.72179735464198 29.61381960154852 20.97414543459592 66-67 19.59119621825485 29.63759568011027 29.61636843518372 21.154839666451164 68-69 19.834582574467404 29.5151755415613 29.416308921355483 21.233932962615818 70-71 19.707393859031843 29.14110191587816 29.51892712374018 21.632577101349817 72-73 19.626749792089516 28.989021938675613 29.59889269492698 21.785335574307886 74-75 19.91685519155874 28.869315901808097 29.284361448258885 21.929467458374287 76-77 19.67754004578877 28.800303190674754 29.716078604702556 21.806078158833916 78-79 19.647395448650474 28.680791009737383 29.56235085209374 22.109462689518402 80-81 19.629095089333745 28.72753813392162 29.54900343416113 22.0943633425835 82-83 19.750544738331744 28.622897620210765 29.66042059227208 21.966137049185413 84-85 19.859934474536185 28.502510517030803 29.443421282205378 22.194133726227633 86-87 19.817638489192944 28.406871463922684 29.386335196624557 22.389154850259818 88-89 19.972201059616516 28.149819617057 29.346395345906828 22.53158397741966 90-91 19.996142154810094 28.027148373889172 29.452127824563036 22.524581646737698 92-93 19.780514826686776 27.95770422618488 29.562745289995902 22.699035657132445 94-95 19.83369099752446 28.10193720230999 29.40532754867238 22.659044251493174 96-97 19.912125021687654 28.047200087622965 29.192792817243113 22.847882073446268 98-99 20.06657031998922 27.791266958808404 29.081478853649006 23.060683867553365 100-101 20.118100581294474 27.721261691075476 29.12966734292109 23.030970384708958 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3621.0 1 2093.0 2 616.0 3 698.0 4 524.0 5 385.5 6 673.5 7 1360.0 8 1988.0 9 2148.0 10 1631.5 11 948.5 12 787.0 13 786.5 14 818.5 15 871.0 16 903.5 17 947.0 18 1031.0 19 1158.5 20 1295.5 21 1447.0 22 1684.0 23 1997.0 24 2371.0 25 2911.5 26 3689.0 27 4680.5 28 5525.0 29 6423.0 30 7638.5 31 8874.0 32 10282.0 33 11977.5 34 13356.5 35 14487.5 36 15733.5 37 16899.0 38 17736.0 39 18244.5 40 18657.5 41 18983.0 42 19593.0 43 20200.5 44 20549.0 45 21085.0 46 21282.5 47 21191.5 48 21831.5 49 21394.5 50 19935.0 51 18242.5 52 15729.5 53 13519.0 54 11550.5 55 9560.5 56 7886.0 57 6466.5 58 5091.0 59 3916.0 60 2986.0 61 2203.0 62 1475.0 63 914.0 64 600.5 65 420.0 66 288.0 67 208.0 68 163.5 69 127.0 70 100.0 71 75.0 72 51.0 73 33.5 74 21.5 75 18.0 76 16.5 77 11.5 78 9.5 79 8.0 80 6.5 81 7.0 82 4.5 83 1.5 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.009886652437641395 3 0.005815677904494937 4 0.0 5 3.877118602996625E-4 6 0.0 7 0.0 8 0.0 9 1.9385593014983126E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0011631355808989876 26-27 0.02035487266573228 28-29 1.9385593014983126E-4 30-31 0.0 32-33 0.0029078389522474686 34-35 0.013957626970787848 36-37 0.006591101625094262 38-39 0.0198702328403577 40-41 0.02151800824663127 42-43 0.016283898132585826 44-45 0.009111228717042069 46-47 0.001744703371348481 48-49 9.692796507491563E-5 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 9.692796507491563E-5 68-69 9.692796507491563E-5 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 9.692796507491563E-5 82-83 5.815677904494938E-4 84-85 0.003295550812547131 86-87 6.784957555244093E-4 88-89 0.0 90-91 0.0029078389522474686 92-93 6.784957555244093E-4 94-95 0.0 96-97 9.692796507491563E-5 98-99 2.907838952247469E-4 100-101 0.0030047669173223846 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 515847.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.20044689150086 #Duplication Level Percentage of deduplicated Percentage of total 1 79.53394594940694 54.24250656794028 2 13.278269092133979 18.111677720582815 3 3.9430742724767454 8.06758282527881 4 1.5172880481792546 4.13918891795833 5 0.6842213464826365 2.3332100801410127 6 0.35289969698960294 1.4440750225539696 7 0.2087729770583117 0.9966887233972137 8 0.12054955832965547 0.6577227000524443 9 0.08235511316624068 0.5054989969563967 >10 0.25308094288414235 2.787817147818698 >50 0.012771502897219466 0.6230711814962544 >100 0.009365766663247546 1.2430451844357084 >500 0.0011352444440298193 0.5572605241342403 >1k 0.0022704888880596386 4.290654407253806 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4612 0.8940635498510217 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4403 0.8535476604497071 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3652 0.7079618569071837 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2776 0.5381440620959316 No Hit CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT 2586 0.5013114353674636 No Hit ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT 1600 0.31016948823973 TruSeq Adapter, Index 3 (95% over 21bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1447 0.2805095309268058 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1091 0.21149681979346588 No Hit ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT 867 0.1680730914399037 RNA PCR Primer, Index 3 (95% over 23bp) ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 855 0.16574682027810572 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 627 0.12154766820394419 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 530 0.10274364297941055 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 1.9385593014983126E-4 0.0 9 0.0 0.0 0.0 1.9385593014983126E-4 0.0 10-11 0.0 0.0 0.0 2.907838952247469E-4 0.0 12-13 0.0 0.0 0.0 3.877118602996625E-4 0.0 14-15 0.0 0.0 0.0 3.877118602996625E-4 0.0 16-17 0.0 0.0 0.0 3.877118602996625E-4 0.0 18-19 0.0 0.0 0.0 5.815677904494938E-4 0.0 20-21 0.0 0.0 0.0 0.001260063545973903 0.0 22-23 0.0 0.0 0.0 0.0027139830220976376 0.0 24-25 0.0 0.0 0.0 0.009402012612266815 0.0 26-27 0.0 0.0 0.0 0.02083951249110686 0.0 28-29 0.0 0.0 0.0 0.047979342712083234 0.0 30-31 0.0 0.0 0.0 0.11902754111199638 0.0 32-33 0.0 0.0 0.0 0.23340253990039683 0.0 34-35 0.0 0.0 0.0 0.40079713558477614 0.0 36-37 0.0 0.0 0.0 0.6384645059484693 0.0 38-39 0.0 0.0 0.0 0.8924157744447481 0.0 40-41 0.0 0.0 0.0 1.1502441615440238 0.0 42-43 0.0 0.0 0.0 1.39014087510444 0.0 44-45 0.0 0.0 0.0 1.6833479694560596 0.0 46-47 0.0 0.0 0.0 2.0120306990250985 0.0 48-49 0.0 0.0 0.0 2.3280158651693235 0.0 50-51 0.0 0.0 0.0 2.6118209469086766 0.0 52-53 0.0 0.0 0.0 2.882928465223215 0.0 54-55 0.0 0.0 0.0 3.20502009316716 0.0 56-57 0.0 0.0 0.0 3.56685218679182 0.0 58-59 0.0 0.0 0.0 3.940024852330245 0.0 60-61 0.0 0.0 0.0 4.285282263927094 0.0 62-63 0.0 0.0 0.0 4.627631836571696 0.0 64-65 0.0 0.0 0.0 4.99721816740235 0.0 66-67 0.0 0.0 0.0 5.420308734954356 0.0 68-69 0.0 0.0 0.0 5.873156187784362 0.0 70-71 0.0 0.0 0.0 6.319412538989274 0.0 72-73 0.0 0.0 0.0 6.775361686701677 0.0 74-75 0.0 0.0 0.0 7.240325135166048 0.0 76-77 0.0 0.0 0.0 7.734173117222742 0.0 78-79 0.0 0.0 0.0 8.266986141239553 0.0 80-81 0.0 0.0 0.0 8.825000436175843 0.0 82-83 0.0 0.0 0.0 9.367409328735071 0.0 84-85 0.0 0.0 0.0 9.944421504826042 0.0 86-87 0.0 0.0 0.0 10.53490666806243 0.0 88-89 0.0 0.0 0.0 11.166973928315954 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGCGTCA 25 0.005020401 56.998024 3 GTAGCGC 25 0.005020401 56.998024 3 GGTATCA 2725 0.0 34.87132 1 GCCGCTA 35 0.0083925985 27.141916 66-67 GTATCAA 3960 0.0 26.99555 1 ATCAACG 4420 0.0 24.179026 3 TATCAAC 4465 0.0 23.942303 2 TCAACGC 4485 0.0 23.828606 4 CAACGCA 4525 0.0 23.617964 5 AACGCAG 4635 0.0 22.954975 6 GAGTACT 3235 0.0 22.023964 12-13 GTCTAGA 175 4.033118E-6 21.71985 1 GTACTTT 3270 0.0 21.425097 14-15 ACGCAGA 5105 0.0 20.841589 7 CGCAGAG 5060 0.0 20.745329 8 ATTAGGG 185 6.5425465E-6 20.539827 3 TGTATAC 235 1.1292468E-7 20.212065 3 GTATAAT 145 6.129157E-4 19.660206 1 GTATAGA 170 7.9273916E-5 19.563833 1 GCAGAGT 5330 0.0 19.248863 9 >>END_MODULE