FastQCFastQC Report
Thu 26 May 2016
SRR938097_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938097_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences527042
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46690.8858876522174702No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44290.8403504844016226No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39110.7420660971990847No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26940.5111547087328904No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15130.2870738954390732No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10560.20036353838972987No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9000.17076437930942887No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6340.12029401831353098No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6060.11498134873501543No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTCA500.00160646638.04119
GGTATCA29650.036.2464371
TATAACG600.003939160631.6828462
TCGCCGC300.003959296731.6527890-91
TAGCTCG700.00834339827.1722159
TGTCGGA350.00839755927.13868130-31
GTATCAA41700.026.0014341
ATCAACG47450.022.735533
TATCAAC47350.022.6831783
TCAACGC47800.022.5690574
CAACGCA48100.022.5270986
AACGCAG48850.022.2912277
GAGTACT32300.021.86185512-13
GACCGGG550.002881365121.58553934-35
ACTATCC1353.7645904E-421.1339448
CGCAGAA1353.7645904E-421.1339449
GTATGGA1604.9631293E-520.7918682
GTACTTT33400.020.76619114-15
GTACATG20350.020.655031
TACATGG20500.020.4006632
Produced by FastQC (version 0.11.5)