##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938097_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 527042 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.672659863919762 33.0 31.0 34.0 30.0 34.0 2 31.863589619043644 34.0 31.0 34.0 30.0 34.0 3 31.91813555655906 34.0 31.0 34.0 30.0 34.0 4 35.40434538423883 37.0 35.0 37.0 33.0 37.0 5 35.42760349270077 37.0 35.0 37.0 33.0 37.0 6 35.46837633433389 37.0 35.0 37.0 33.0 37.0 7 35.41704456191347 37.0 35.0 37.0 33.0 37.0 8 35.40827486234494 37.0 35.0 37.0 33.0 37.0 9 37.05151581847367 39.0 37.0 39.0 34.0 39.0 10-11 36.99023038012151 39.0 37.0 39.0 34.0 39.0 12-13 36.90995878886312 39.0 37.0 39.0 33.5 39.0 14-15 38.14233116146341 40.0 38.0 41.0 33.0 41.0 16-17 38.061877421533765 40.0 38.0 41.0 33.0 41.0 18-19 38.020927174684374 40.0 38.0 41.0 33.5 41.0 20-21 37.94997931853628 40.0 38.0 41.0 33.5 41.0 22-23 37.79812235078039 40.0 38.0 41.0 33.0 41.0 24-25 37.74423385612532 40.0 38.0 41.0 33.0 41.0 26-27 37.71806136892316 40.0 38.0 41.0 33.0 41.0 28-29 37.601047924074365 40.0 38.0 41.0 33.0 41.0 30-31 37.382722819054266 40.0 37.0 41.0 33.0 41.0 32-33 37.124730097411586 40.0 36.5 41.0 32.0 41.0 34-35 36.912100933132464 40.0 36.0 41.0 31.0 41.0 36-37 36.700815115303904 40.0 35.5 41.0 31.0 41.0 38-39 36.46418881227682 39.5 35.0 41.0 30.0 41.0 40-41 36.21657002667719 39.0 35.0 41.0 30.0 41.0 42-43 35.90145282539152 39.0 35.0 41.0 29.5 41.0 44-45 35.577858121364144 39.0 35.0 41.0 27.5 41.0 46-47 35.308562695193174 39.0 35.0 41.0 25.5 41.0 48-49 34.99847640226016 39.0 35.0 41.0 23.5 41.0 50-51 34.357449690916475 38.5 34.5 40.5 22.5 40.5 52-53 34.422626659734895 38.5 35.0 40.0 22.0 41.0 54-55 34.57090231898027 39.0 35.0 41.0 19.5 41.0 56-57 34.39340887443505 39.0 35.0 41.0 18.0 41.0 58-59 34.17442348048163 39.0 35.0 41.0 14.0 41.0 60-61 33.8524472432937 38.5 34.0 40.0 8.5 41.0 62-63 33.490386914135875 38.0 34.0 40.0 7.5 41.0 64-65 33.11978457124859 38.0 33.5 40.0 2.0 41.0 66-67 32.729538632594746 37.0 33.0 40.0 2.0 41.0 68-69 32.27372296705006 36.5 32.5 39.5 2.0 41.0 70-71 31.814756698707125 36.0 32.0 39.0 2.0 40.5 72-73 31.338038524443974 35.5 31.0 39.0 2.0 40.0 74-75 30.8326651386417 35.0 31.0 38.0 2.0 40.0 76-77 30.2909739641243 35.0 30.5 37.0 2.0 39.0 78-79 29.722505986240186 35.0 29.5 36.5 2.0 39.0 80-81 29.188579847526384 34.0 29.0 36.0 2.0 38.5 82-83 28.652941321564505 34.0 29.0 35.5 2.0 37.0 84-85 28.142424133181038 34.0 27.5 35.0 2.0 37.0 86-87 27.643910731972028 34.0 26.5 35.0 2.0 36.0 88-89 27.1064791800274 34.0 26.0 35.0 2.0 36.0 90-91 26.518051692275 33.0 25.0 35.0 2.0 35.5 92-93 25.950562763498922 33.0 23.5 35.0 2.0 35.0 94-95 25.42893924962337 32.5 20.0 35.0 2.0 35.0 96-97 24.66336838430334 32.0 12.0 34.5 2.0 35.0 98-99 23.705829895909623 31.5 2.0 34.0 2.0 35.0 100-101 21.020498366354104 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6705685986069305 1101 2 0.6111097240394763 1101 3 0.5959196490858076 1101 4 0.39887043981054404 1101 5 0.5255136728216598 1101 6 0.4830182775109293 1101 7 0.485264073670848 1101 8 0.41579370559993833 1101 9 0.5181799327791339 1101 10-11 0.59205091394346 1101 12-13 0.4269422913476859 1101 14-15 0.7154793592221509 1101 16-17 0.8627265300331004 1101 18-19 0.7427341045646827 1101 20-21 0.9030141164901551 1101 22-23 0.9645821138415798 1101 24-25 0.7928838592423304 1101 26-27 0.8335560928985117 1101 28-29 0.7873290611260302 1101 30-31 0.976180673438499 1101 32-33 1.0323720326159034 1101 34-35 1.0820983752474191 1101 36-37 1.071193119524466 1101 38-39 1.1849915869595762 1101 40-41 1.0073146371992436 1101 42-43 1.2826107395877457 1101 44-45 1.4702040650590789 1101 46-47 1.5727807499106206 1101 48-49 1.3745784882349241 1101 50-51 1.488641125326886 1101 52-53 1.2673801662518969 1101 54-55 1.2647019296524888 1101 56-57 1.029157934651856 1101 58-59 1.1254925083130232 1101 60-61 1.069399593155346 1101 62-63 1.1237498714169547 1101 64-65 1.154789303972585 1101 66-67 1.2245334105726755 1101 68-69 1.267947191747325 1101 70-71 1.2088725520433137 1101 72-73 1.3146615925712872 1101 74-75 1.3271839873021385 1101 76-77 1.4611872143239637 1101 78-79 1.7681614418996467 1101 80-81 1.6686088109601336 1101 82-83 1.4599135826052319 1101 84-85 1.4227516386495473 1101 86-87 1.273837808831896 1101 88-89 1.0735052339753217 1101 90-91 1.4610725830153903 1101 92-93 1.7498788590666159 1101 94-95 1.8641440090031693 1101 96-97 1.9513197554594512 1101 98-99 1.9820342836226779 1101 100-101 1.6031923733886941 1102 1 0.5058992385410441 1102 2 0.3108119555970852 1102 3 0.34524345642816456 1102 4 0.2889641564578014 1102 5 0.20030764727498251 1102 6 0.20223871875405308 1102 7 0.014317209011721843 1102 8 0.22414486767662112 1102 9 0.32884598863874004 1102 10-11 0.3448105110057327 1102 12-13 0.052000163692255796 1102 14-15 0.4457991069718261 1102 16-17 0.5395722313971802 1102 18-19 0.34100987420594464 1102 20-21 0.3570086484673709 1102 22-23 0.4131890351303156 1102 24-25 0.168900584959097 1102 26-27 0.07633240593062851 1102 28-29 -0.048717977515636335 1102 30-31 0.2444598057675833 1102 32-33 0.4717608031879976 1102 34-35 0.18364667367865195 1102 36-37 0.12449415805892272 1102 38-39 -0.009249932000621186 1102 40-41 0.42911752521199276 1102 42-43 0.16880081429790295 1102 44-45 0.32965346148098007 1102 46-47 0.06541695095061328 1102 48-49 0.26347577030595204 1102 50-51 0.44206789914471045 1102 52-53 0.6163906100779499 1102 54-55 0.39264976413026886 1102 56-57 0.49832842292386914 1102 58-59 0.45664171905607986 1102 60-61 0.42473091537393515 1102 62-63 0.24424374791371406 1102 64-65 0.4766399518991662 1102 66-67 0.43587833949325727 1102 68-69 0.46940386911936116 1102 70-71 0.4379714979137894 1102 72-73 0.35972160923851604 1102 74-75 0.4112779866101022 1102 76-77 0.40666537391030033 1102 78-79 0.18202058914151564 1102 80-81 0.09792657521134984 1102 82-83 -0.19590069955838985 1102 84-85 -0.028942619926525026 1102 86-87 -0.37100875026125024 1102 88-89 -0.11300453974990177 1102 90-91 0.11714515859058139 1102 92-93 0.28544050103296215 1102 94-95 -0.07314430005220629 1102 96-97 0.3306479976509493 1102 98-99 0.710402210403803 1102 100-101 0.08790189076988852 1103 1 0.6549400416467854 1103 2 0.5998761179961924 1103 3 0.5433056753718901 1103 4 0.42298691994731996 1103 5 0.4937315806206044 1103 6 0.6510066815305038 1103 7 0.6207131159339241 1103 8 0.5598235875495163 1103 9 0.5803224945802228 1103 10-11 0.6488856201182216 1103 12-13 0.5744704798938685 1103 14-15 0.5318833044571605 1103 16-17 0.7102180165578886 1103 18-19 0.8561201215591083 1103 20-21 0.8470667629846531 1103 22-23 0.7825377647914138 1103 24-25 0.8842742154377774 1103 26-27 0.9041261821448145 1103 28-29 0.8354691048897109 1103 30-31 0.9399804011931323 1103 32-33 1.120217623959796 1103 34-35 1.1096109538199457 1103 36-37 1.1876871937740532 1103 38-39 1.2282511910423253 1103 40-41 1.031345169564382 1103 42-43 1.0940250682427362 1103 44-45 1.1101990475675407 1103 46-47 1.2575707818274395 1103 48-49 1.1753724598117685 1103 50-51 1.2194689649715755 1103 52-53 1.2496307899539687 1103 54-55 1.3357528618373706 1103 56-57 1.307765766584474 1103 58-59 1.3450774502634388 1103 60-61 1.553313422384086 1103 62-63 1.7674800976221903 1103 64-65 1.6648335786895316 1103 66-67 1.6636219925273679 1103 68-69 1.5892431912269913 1103 70-71 1.5944574847020796 1103 72-73 1.63970212739871 1103 74-75 1.486521864252797 1103 76-77 1.43192083662764 1103 78-79 1.571898543926622 1103 80-81 1.4975123496854117 1103 82-83 1.6296186191446473 1103 84-85 1.8591502876434909 1103 86-87 1.8665940377224288 1103 88-89 1.3318386834542935 1103 90-91 1.4984738011508334 1103 92-93 1.712315037038902 1103 94-95 2.0875501424219856 1103 96-97 2.0830985955826584 1103 98-99 2.066290974772844 1103 100-101 2.1337433218339683 1104 1 0.245552263968289 1104 2 0.36487107606519587 1104 3 0.15034140287785647 1104 4 0.15453494168080795 1104 5 0.29523692676938396 1104 6 0.19116981033588587 1104 7 0.22151557009915734 1104 8 0.38075206871566536 1104 9 0.05687219695737866 1104 10-11 0.2008834586828243 1104 12-13 0.10521349458566931 1104 14-15 0.22866327629041905 1104 16-17 0.4168457093106923 1104 18-19 0.35238228495170887 1104 20-21 0.19348784293647725 1104 22-23 0.06031625170154342 1104 24-25 0.4524721824971607 1104 26-27 0.4001202935097936 1104 28-29 0.23827471611215856 1104 30-31 0.36766166605831785 1104 32-33 0.3903396089063378 1104 34-35 0.577840406355584 1104 36-37 0.7166963798744916 1104 38-39 0.44877078649650315 1104 40-41 0.19860750740354405 1104 42-43 0.6103230570015725 1104 44-45 0.6101096968916764 1104 46-47 0.5118755697472324 1104 48-49 0.8876483917180451 1104 50-51 0.8937708596313811 1104 52-53 0.40148864579303023 1104 54-55 0.5701026654786219 1104 56-57 0.2679813144530314 1104 58-59 0.5729344381245127 1104 60-61 0.4707427608912482 1104 62-63 0.3754348606514384 1104 64-65 0.37561607787048246 1104 66-67 0.35465799234015094 1104 68-69 0.3841621161256228 1104 70-71 0.4420018302934743 1104 72-73 0.128854529868196 1104 74-75 0.21825496952917334 1104 76-77 0.2761102770796704 1104 78-79 0.37473564062532816 1104 80-81 0.296345685146143 1104 82-83 0.49657333537115633 1104 84-85 0.6885458090472341 1104 86-87 0.6026244850289295 1104 88-89 0.678766083902751 1104 90-91 0.6701866536073418 1104 92-93 0.6515267938859246 1104 94-95 0.8344303146560499 1104 96-97 0.39561534883438654 1104 98-99 1.0552902046551118 1104 100-101 1.3450129949381413 1105 1 0.3380240292041883 1105 2 0.3616398073261671 1105 3 0.27461708602710644 1105 4 0.26115904259581413 1105 5 0.35909720546631263 1105 6 0.5189027956864294 1105 7 0.34060646506738834 1105 8 0.39398395885253024 1105 9 0.4488111135502564 1105 10-11 0.31822473037349397 1105 12-13 0.27337928352790186 1105 14-15 0.46998506715898003 1105 16-17 0.5501491378732482 1105 18-19 0.6412507194952184 1105 20-21 0.6042570557807778 1105 22-23 0.5752783464336488 1105 24-25 0.6744102446680174 1105 26-27 0.44901459373816266 1105 28-29 0.5263605777579272 1105 30-31 0.32405313115906154 1105 32-33 0.733595888908205 1105 34-35 0.861382296337105 1105 36-37 0.7686258201503904 1105 38-39 0.8057890866627275 1105 40-41 0.7483562296542488 1105 42-43 0.6963555871782035 1105 44-45 0.9167462257633687 1105 46-47 0.9928569122772473 1105 48-49 0.9527572916885276 1105 50-51 0.9412262354993999 1105 52-53 1.0071728824834665 1105 54-55 1.0041723552457569 1105 56-57 1.3282627299972987 1105 58-59 1.3386771638861106 1105 60-61 1.2654795212336367 1105 62-63 1.1367093476098518 1105 64-65 1.0700883557596015 1105 66-67 1.0328574144401728 1105 68-69 1.1535361354821632 1105 70-71 1.2513725096366493 1105 72-73 1.2827611803785182 1105 74-75 1.2987842451346445 1105 76-77 1.1476789959126847 1105 78-79 1.5102780432600547 1105 80-81 1.1293508427481704 1105 82-83 1.3232072233020595 1105 84-85 1.2939562423168773 1105 86-87 1.2846493875471445 1105 88-89 1.4032848889547118 1105 90-91 1.0232047052144289 1105 92-93 1.2074700757985894 1105 94-95 1.0925123702400867 1105 96-97 0.9623942554848988 1105 98-99 0.5112991217140355 1105 100-101 0.7399559816868297 1106 1 -0.2971254724569441 1106 2 -0.2220589745121515 1106 3 -0.1255933237181921 1106 4 -0.14882988320706403 1106 5 -0.169441000513757 1106 6 -0.21299089202121024 1106 7 -0.0978431473357162 1106 8 -0.23577240881080996 1106 9 -0.15878578567455293 1106 10-11 -0.6077719473673682 1106 12-13 -0.358359365420057 1106 14-15 -0.32264505465719395 1106 16-17 0.015713921926405305 1106 18-19 0.05926843821061567 1106 20-21 -0.1187385145403752 1106 22-23 0.0529472279397325 1106 24-25 -0.10153106208723983 1106 26-27 0.04202123825643156 1106 28-29 0.02023100965161717 1106 30-31 -0.03217610472133714 1106 32-33 0.17012966520942285 1106 34-35 0.0305572686516129 1106 36-37 -0.07913719203122582 1106 38-39 0.09893969574356731 1106 40-41 0.3133894522456089 1106 42-43 0.30043089171862647 1106 44-45 0.43765430992726095 1106 46-47 0.5577406332074801 1106 48-49 0.41986138914145954 1106 50-51 0.4686348733731549 1106 52-53 0.49833567318114547 1106 54-55 0.4336297195868397 1106 56-57 0.6418452327658528 1106 58-59 0.6748621029780182 1106 60-61 0.48107199077959706 1106 62-63 0.8559120054171601 1106 64-65 0.8403264509976864 1106 66-67 0.7952992749052896 1106 68-69 0.44869117424185134 1106 70-71 0.6800989769677734 1106 72-73 0.8147157761703205 1106 74-75 1.0296701809043007 1106 76-77 1.0608683646834507 1106 78-79 0.9557929934261686 1106 80-81 1.0581492923605715 1106 82-83 0.819275883324206 1106 84-85 0.8811157107554131 1106 86-87 1.139660461744267 1106 88-89 0.8174377128749768 1106 90-91 0.5036421360006962 1106 92-93 0.7370769418008045 1106 94-95 0.7854027356885922 1106 96-97 0.9828316862711652 1106 98-99 1.1100034406509103 1106 100-101 0.39863261513090364 1107 1 0.1499552728846716 1107 2 0.039597340187640384 1107 3 0.05099052721348585 1107 4 -0.10474085629194718 1107 5 -0.06468944771872742 1107 6 -0.04264927711437849 1107 7 -0.1353793792197706 1107 8 0.14491082100285269 1107 9 -0.22501766973252302 1107 10-11 0.041788094040498436 1107 12-13 -0.060227895440760904 1107 14-15 -0.010129526706883496 1107 16-17 -0.036470343083948364 1107 18-19 0.0911369682313179 1107 20-21 0.19702753100827408 1107 22-23 -0.022358155083040288 1107 24-25 -0.031163567263227776 1107 26-27 0.0727717558547809 1107 28-29 0.03383867010710162 1107 30-31 0.17263755987493568 1107 32-33 0.1660613422280548 1107 34-35 0.3528088214466365 1107 36-37 0.40666508540148527 1107 38-39 0.6175939400499928 1107 40-41 0.4812724750199422 1107 42-43 0.5832362747414024 1107 44-45 0.6267226329086313 1107 46-47 0.4208565752364706 1107 48-49 0.43087070591165144 1107 50-51 0.24930257523650567 1107 52-53 0.5079733543405638 1107 54-55 0.6216731978477128 1107 56-57 0.6317468890805529 1107 58-59 0.5334024756488347 1107 60-61 0.5521537713178972 1107 62-63 0.5747991689782381 1107 64-65 0.7829506746001798 1107 66-67 0.6762050720067379 1107 68-69 0.5517708843867766 1107 70-71 0.7003318962224334 1107 72-73 0.547443519441547 1107 74-75 0.6925532036786315 1107 76-77 0.8989428988449397 1107 78-79 0.429809556573165 1107 80-81 0.2959753793170954 1107 82-83 0.6615119081689294 1107 84-85 0.8384034954345019 1107 86-87 0.8770828219927118 1107 88-89 0.9417286031441279 1107 90-91 1.026680438278131 1107 92-93 0.8789817037055663 1107 94-95 1.010211224301429 1107 96-97 0.9722105682587419 1107 98-99 0.7536525089738966 1107 100-101 1.0365518697892888 1108 1 0.090503815817506 1108 2 -0.03512605080503306 1108 3 0.0060707827846258056 1108 4 -0.02582937117429651 1108 5 0.12447605017748486 1108 6 -0.0010605284779430235 1108 7 -0.03131984984467806 1108 8 -0.10524501505761208 1108 9 0.023579805723301206 1108 10-11 -0.18391122028083373 1108 12-13 -0.21245050725312353 1108 14-15 -0.17716630401714895 1108 16-17 0.02709129007796207 1108 18-19 0.11791155136985054 1108 20-21 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-1.819529873737455 2316 98-99 -1.92325663260916 2316 100-101 -1.9320116425721743 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5532.0 3 795.0 4 656.0 5 590.0 6 590.0 7 707.0 8 787.0 9 874.0 10 1163.0 11 1479.0 12 1991.0 13 2666.0 14 3223.0 15 4216.0 16 5550.0 17 7576.0 18 6538.0 19 4400.0 20 4184.0 21 4283.0 22 4140.0 23 4401.0 24 4811.0 25 5410.0 26 5902.0 27 6846.0 28 7971.0 29 9322.0 30 11206.0 31 13728.0 32 16743.0 33 21645.0 34 29806.0 35 45627.0 36 64612.0 37 103266.0 38 101150.0 39 12655.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.047948561981705 20.888495662968097 15.437888631046246 21.625667144003952 2 15.354111735640105 22.430651655191657 40.20884705114114 22.006389558027095 3 18.65779736920539 26.12342219716342 31.399818893198244 23.818961540432948 4 12.313940065918377 19.632170901745166 37.95679612387794 30.09709290845851 5 12.616424023652234 38.27537687678849 35.18782601100201 13.920373088557264 6 28.914813639998403 38.12274327088726 18.44567990107905 14.516763188035275 7 25.264868723845403 35.18178158868071 22.08853260828289 17.464817079191004 8 24.061741847547523 35.55623474203359 21.447337947582835 18.934685462836054 9 25.047411673807684 17.60733832097181 22.201951899219583 35.14329810600093 10-11 23.078003909546872 28.238833629698124 28.59504100846942 20.088121452285584 12-13 24.450379076748916 26.696348270055736 28.48151816842727 20.371754484768076 14-15 21.72395561921522 28.402999850975636 27.984023069351384 21.889021460457762 16-17 19.5830794144393 30.957222022884025 29.129385677645452 20.33031288503122 18-19 19.09880920175226 29.490790179355386 32.188572323551604 19.221828295340746 20-21 20.6443301171259 28.50012959135526 32.07912952584754 18.776410765671297 22-23 20.036566819882783 28.820197946866095 31.706052890886205 19.43718234236492 24-25 19.651862465543747 28.864631433218722 32.1542956491354 19.329210452102128 26-27 19.7191361628566 28.98709218686663 31.981416242560947 19.312355407715827 28-29 19.32671477745438 29.423335061068805 32.211824165892985 19.038125995583833 30-31 19.831906605920388 29.228655812968807 31.968978765701202 18.970458815409604 32-33 19.449489200635306 29.183964668879923 31.88288022298402 19.483665907500743 34-35 19.646532540423948 29.34960924149559 31.760900605314923 19.242957612765537 36-37 19.70434277403639 29.13795755006256 31.594900995080604 19.562798680820443 38-39 19.581798666227577 28.995551877521464 32.03511805508763 19.387531401163326 40-41 19.796445579942183 28.972813223065195 31.83413465644972 19.3966065405429 42-43 19.619147946739552 29.03149882792852 31.541819700291356 19.807533525040572 44-45 19.775261494315586 29.24940717200884 31.24675593326166 19.728575400413913 46-47 19.726261129004403 29.058587292579652 30.898063918586267 20.31708765982968 48-49 19.718101354833014 28.98537934280587 31.078884362340254 20.217634940020858 50-51 19.939192305283477 29.180196884839262 30.538785781931153 20.341825027946108 52-53 19.917121121398484 29.293233139784803 30.41020964541618 20.37943609340053 54-55 20.080677225621994 29.026625761864587 30.422867467112784 20.469829545400632 56-57 20.007610306746603 29.12612422829402 30.171924610517404 20.69434085444197 58-59 20.036740460717027 28.985965181961117 30.26106412595753 20.71623023136432 60-61 20.000436516710522 28.771385760255537 30.327947413021672 20.90023031001227 62-63 20.00884343607344 28.791552051496883 30.32596411956098 20.873640392868698 64-65 20.107072740953996 28.661979782950915 30.317602952186384 20.913344523908705 66-67 20.04040170184341 28.597630171664534 30.202976328284226 21.158991798207825 68-69 19.923371283967462 28.59780646030555 30.275554054272032 21.203268201454957 70-71 19.810873089085533 28.28779785969987 30.33252853796774 21.568800513246856 72-73 19.69621910944084 28.179592301560184 30.44148350605474 21.68270508294424 74-75 19.836303592780276 28.189090702044066 30.22139345973543 21.753212245440228 76-77 19.706455585940688 28.141682433830102 30.321346493616907 21.830515486612303 78-79 19.59786741257255 28.089979325022224 30.296480509634883 22.015672752770342 80-81 19.657918533144954 28.07730517382562 30.297437804879205 21.967338488150215 82-83 19.745979029273617 27.967262893201124 30.277174170897187 22.009583906628077 84-85 19.872984605574786 27.727329659318638 30.174648539503252 22.225037195603328 86-87 19.830264849453744 27.62546311889525 30.188093871413052 22.356178160237953 88-89 20.18542701931144 27.32614200190571 30.19505047092275 22.2933805078601 90-91 20.109192418416537 27.25463265730343 30.173137943700983 22.46303698057905 92-93 20.03646029557323 27.103513265493017 30.237940913822932 22.62208552511082 94-95 19.95500882738197 27.05393245629022 30.246881940885018 22.744176775442792 96-97 20.138450602233547 26.86737005545427 29.991145159591177 23.00303418272101 98-99 20.226045531757407 26.63114341839301 30.082560710591494 23.060250339258094 100-101 20.60837585882487 26.193041974323155 30.241088652583365 22.95749351426861 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3865.0 1 2201.0 2 641.5 3 735.0 4 545.5 5 450.5 6 752.5 7 1422.0 8 2013.0 9 2162.0 10 1682.0 11 1022.0 12 895.0 13 930.5 14 931.5 15 963.0 16 995.5 17 1046.0 18 1122.5 19 1215.0 20 1376.0 21 1608.5 22 1864.0 23 2112.0 24 2487.0 25 3106.5 26 3975.0 27 4981.5 28 5914.0 29 6813.0 30 7975.0 31 9368.5 32 10791.5 33 12162.0 34 13449.0 35 14786.0 36 16076.5 37 17166.5 38 18029.5 39 18533.5 40 18964.5 41 19278.0 42 19590.5 43 20090.0 44 20565.0 45 20811.5 46 20705.5 47 20675.0 48 21437.5 49 21162.0 50 19491.0 51 17918.0 52 16054.0 53 14000.5 54 11864.5 55 10000.0 56 8337.0 57 6734.0 58 5364.0 59 4244.5 60 3376.0 61 2609.0 62 1871.0 63 1274.0 64 908.5 65 678.0 66 507.0 67 411.0 68 332.5 69 286.5 70 238.5 71 191.0 72 164.5 73 144.5 74 122.0 75 90.5 76 70.5 77 50.5 78 41.0 79 38.5 80 38.5 81 28.5 82 13.0 83 9.0 84 8.0 85 7.0 86 3.5 87 1.5 88 1.5 89 2.5 90 2.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4942680090011802 2 0.046485858812011185 3 0.053316433984388345 4 0.06261360574679058 5 0.07798239988463918 6 0.106443129769544 7 0.06944418091916772 8 0.05084983739436326 9 0.05179852839052675 10-11 0.07257486120650726 12-13 0.10729695166609113 14-15 0.053601041283237394 16-17 0.05673172157057691 18-19 0.05587789967402977 20-21 0.07333381400343805 22-23 0.06356229674295408 24-25 0.08889234634051935 26-27 0.0655545478348974 28-29 0.06470072593835026 30-31 0.07029800281571488 32-33 0.06972878821801677 34-35 0.07096208651302932 36-37 0.06659810793067725 38-39 0.03614512695382911 40-41 0.03889633084270324 42-43 0.03737842524884165 44-45 0.022483976609074797 46-47 0.029029944482602904 48-49 0.02855559898452116 50-51 0.025614656896414328 52-53 0.024381358601401785 54-55 0.024191620402169087 56-57 0.02371727490408734 58-59 0.01944816542135162 60-61 0.027227431589892267 62-63 0.018499474425188126 64-65 0.02096607101521321 66-67 0.015938008735546693 68-69 0.016222616034395742 70-71 0.037947639846539746 72-73 0.034532352260351167 74-75 0.028840206283370206 76-77 0.019543034520967967 78-79 0.014514972241301452 80-81 0.01736104522979193 82-83 0.02219936931022575 84-85 0.018594343524804473 86-87 0.008443349865855094 88-89 0.03870659264347054 90-91 0.050375491896281516 92-93 0.03178114837147704 94-95 0.05160879019129405 96-97 0.040129629137715776 98-99 0.029788897279533703 100-101 0.16829778271940377 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 527042.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.55607933572584 #Duplication Level Percentage of deduplicated Percentage of total 1 80.90124329363682 57.08074540184699 2 12.390338481102154 17.48427409738281 3 3.716356504989377 7.86634633217614 4 1.404778670779749 3.964627013786859 5 0.6287247945415927 2.2180178242007274 6 0.32473738764970966 1.3747318131773565 7 0.18978720766868054 0.9373448896824111 8 0.11696462936325688 0.6602052535062177 9 0.07486856456376129 0.47541891430013894 >10 0.23148456081196184 2.603898611078706 >50 0.010761511746972423 0.5428923331409626 >100 0.007533054272634082 0.9168422867025362 >500 8.071129577811686E-4 0.40621997795898346 >1k 0.0016142259155623373 3.468435251059133 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4669 0.8858876522174702 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4429 0.8403504844016226 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3911 0.7420660971990847 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2694 0.5111547087328904 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1513 0.2870738954390732 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1056 0.20036353838972987 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 900 0.17076437930942887 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 634 0.12029401831353098 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 606 0.11498134873501543 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 6.640836973144455E-4 0.0 22-23 0.0 0.0 0.0 0.002656334789257782 0.0 24-25 0.0 0.0 0.0 0.008443349865855092 0.0 26-27 0.0 0.0 0.0 0.020207118218282416 0.0 28-29 0.0 0.0 0.0 0.04696020431009293 0.0 30-31 0.0 0.0 0.0 0.11649925432887702 0.0 32-33 0.0 0.0 0.0 0.21800919091837084 0.0 34-35 0.0 0.0 0.0 0.3939913707066989 0.0 36-37 0.0 0.0 0.0 0.6285077849583145 0.0 38-39 0.0 0.0 0.0 0.8911054526963695 0.0 40-41 0.0 0.0 0.0 1.144880294170104 0.0 42-43 0.0 0.0 0.0 1.3749378607397513 0.0 44-45 0.0 0.0 0.0 1.661916887079208 0.0 46-47 0.0 0.0 0.0 1.982859051081318 0.0 48-49 0.0 0.0 0.0 2.296496294412969 0.0 50-51 0.0 0.0 0.0 2.5753165781854195 0.0 52-53 0.0 0.0 0.0 2.83829372232194 0.0 54-55 0.0 0.0 0.0 3.138554422607686 0.0 56-57 0.0 0.0 0.0 3.49810831015365 0.0 58-59 0.0 0.0 0.0 3.8572827213011487 0.0 60-61 0.0 0.0 0.0 4.2110495937705155 0.0 62-63 0.0 0.0 0.0 4.551155315895128 0.0 64-65 0.0 0.0 0.0 4.91412449102728 0.0 66-67 9.486909961634937E-5 0.0 0.0 5.340655962902387 0.0 68-69 1.8973819923269873E-4 0.0 0.0 5.793655913570456 0.0 70-71 1.8973819923269873E-4 0.0 0.0 6.247225078836221 0.0 72-73 1.8973819923269873E-4 0.0 0.0 6.700699375002372 0.0 74-75 1.8973819923269873E-4 0.0 0.0 7.158442780651257 0.0 76-77 1.8973819923269873E-4 0.0 0.0 7.645310999882362 0.0 78-79 1.8973819923269873E-4 0.0 0.0 8.167755131469598 0.0 80-81 1.8973819923269873E-4 0.0 0.0 8.700634864014631 0.0 82-83 1.8973819923269873E-4 0.0 0.0 9.238637527938948 0.0 84-85 1.8973819923269873E-4 0.0 0.0 9.807093172840116 0.0 86-87 1.8973819923269873E-4 0.0 0.0 10.39243551747299 0.0 88-89 1.8973819923269873E-4 0.0 0.0 11.00263356620535 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTGTCA 50 0.001606466 38.0411 9 GGTATCA 2965 0.0 36.246437 1 TATAACG 60 0.0039391606 31.682846 2 TCGCCGC 30 0.0039592967 31.65278 90-91 TAGCTCG 70 0.008343398 27.172215 9 TGTCGGA 35 0.008397559 27.138681 30-31 GTATCAA 4170 0.0 26.001434 1 ATCAACG 4745 0.0 22.73553 3 TATCAAC 4735 0.0 22.683178 3 TCAACGC 4780 0.0 22.569057 4 CAACGCA 4810 0.0 22.527098 6 AACGCAG 4885 0.0 22.291227 7 GAGTACT 3230 0.0 21.861855 12-13 GACCGGG 55 0.0028813651 21.585539 34-35 ACTATCC 135 3.7645904E-4 21.133944 8 CGCAGAA 135 3.7645904E-4 21.133944 9 GTATGGA 160 4.9631293E-5 20.791868 2 GTACTTT 3340 0.0 20.766191 14-15 GTACATG 2035 0.0 20.65503 1 TACATGG 2050 0.0 20.400663 2 >>END_MODULE