FastQCFastQC Report
Thu 26 May 2016
SRR938097_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938097_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences527042
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47450.9003077553591554No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44570.8456631539801381No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39220.7441532173906443No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28200.5350617218362104No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT26270.49844224938429954No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT15980.30320164237385255TruSeq Adapter, Index 3 (95% over 21bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14420.2736024832935515No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10730.20358908777668572No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9260.17569757248947904No Hit
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT8570.16260563674242282RNA PCR Primer, Index 3 (95% over 23bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5950.11289422854345575No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5600.10625339157031127No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28800.036.288491
GGCGGGT300.003948795631.66995824-25
TTGCGCA650.005843691529.2282584
GTATCAA40150.028.2781541
TTGCCGT458.946583E-426.38912890-91
TATCAAC46550.024.1861862
TCAACGC46800.023.9509334
ATCAACG46900.023.8998663
CAGCGTG500.00165255523.75246880-81
CAACGCA47400.023.5475565
AACGCAG48150.023.1807696
GAGTACT33200.022.96113212-13
GTACTGC1050.002195751322.6171046
GTCCTAC1703.140789E-622.3552631
GTACTTT33750.022.02403514-15
GTACATG22300.020.6635821
CGCAGAA1150.00372249420.65049
GTAGGCT2551.1892553E-820.4884364
ACGCAGA53800.020.3932297
GTCTAAG1404.843548E-420.3592591
Produced by FastQC (version 0.11.5)