##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938097_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 527042 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.50572440147085 34.0 31.0 34.0 31.0 34.0 2 32.710681501664006 34.0 31.0 34.0 31.0 34.0 3 32.760510547546495 34.0 31.0 34.0 31.0 34.0 4 36.22495171162829 37.0 35.0 37.0 35.0 37.0 5 36.195436037355655 37.0 35.0 37.0 35.0 37.0 6 36.23982528906615 37.0 37.0 37.0 35.0 37.0 7 36.20157406810083 37.0 37.0 37.0 35.0 37.0 8 36.20473510650005 37.0 37.0 37.0 35.0 37.0 9 37.90892376698631 39.0 38.0 39.0 35.0 39.0 10-11 37.90726640381601 39.0 38.0 39.0 35.0 39.0 12-13 37.86497375920705 39.0 38.0 39.0 35.0 39.0 14-15 39.174790623897145 40.5 39.0 41.0 36.0 41.0 16-17 39.126784962109284 40.0 38.5 41.0 36.0 41.0 18-19 39.17084406935311 40.0 38.5 41.0 36.0 41.0 20-21 39.137488093928 40.0 39.0 41.0 35.0 41.0 22-23 38.99183556528702 40.0 38.5 41.0 35.0 41.0 24-25 38.941427817896866 40.0 38.0 41.0 35.0 41.0 26-27 38.87828009911924 40.0 38.0 41.0 35.0 41.0 28-29 38.78958318312392 40.0 38.0 41.0 35.0 41.0 30-31 38.54944672341104 40.0 38.0 41.0 35.0 41.0 32-33 38.33429498977311 40.0 38.0 41.0 34.0 41.0 34-35 38.11809495258443 40.0 37.0 41.0 34.0 41.0 36-37 37.97412824784362 40.0 37.0 41.0 34.0 41.0 38-39 37.89884297646108 40.0 37.0 41.0 33.0 41.0 40-41 37.82394192493197 40.0 37.0 41.0 33.0 41.0 42-43 37.63553189309391 40.0 37.0 41.0 33.0 41.0 44-45 37.353388344761896 40.0 37.0 41.0 33.0 41.0 46-47 36.93674128437581 40.0 37.0 41.0 31.5 41.0 48-49 36.55995157881156 40.0 36.5 41.0 30.5 41.0 50-51 36.24337339339179 40.0 36.0 41.0 30.0 41.0 52-53 35.86086782457565 40.0 35.0 41.0 28.5 41.0 54-55 35.68114685357144 39.0 35.0 41.0 28.0 41.0 56-57 35.45522178498108 39.0 35.0 41.0 27.0 41.0 58-59 35.13632120400272 39.0 35.0 41.0 26.0 41.0 60-61 34.80123405724781 39.0 35.0 40.5 25.5 41.0 62-63 34.44181203775031 38.0 34.5 40.0 23.0 41.0 64-65 34.0478491277735 38.0 34.0 40.0 21.5 41.0 66-67 33.59119481938821 37.5 34.0 40.0 19.0 41.0 68-69 33.10240360350788 37.0 33.5 39.5 12.0 41.0 70-71 32.60327450184236 36.0 33.0 39.0 7.5 40.5 72-73 32.19353580929034 36.0 33.0 39.0 6.0 40.0 74-75 31.7515824165816 35.0 32.0 38.0 2.0 40.0 76-77 30.36504016757678 34.0 30.5 36.0 2.0 39.0 78-79 31.001861331734474 35.0 31.5 37.0 2.0 39.0 80-81 30.801432333666007 35.0 32.0 36.5 2.0 39.0 82-83 30.427132941966676 35.0 31.0 36.0 2.0 37.5 84-85 30.04341779213042 35.0 31.0 35.5 2.0 37.0 86-87 29.616631122377342 34.5 31.0 35.0 2.0 36.5 88-89 29.19325689413747 34.0 30.0 35.0 2.0 36.0 90-91 28.88927353038278 34.0 30.0 35.0 2.0 36.0 92-93 28.56174365610331 34.0 29.0 35.0 2.0 35.5 94-95 28.265671426565625 34.0 29.0 35.0 2.0 35.0 96-97 27.83096982783156 34.0 29.0 35.0 2.0 35.0 98-99 27.300154446894176 34.0 28.0 35.0 2.0 35.0 100-101 25.221376095263757 32.0 22.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2794075499208688 1101 2 0.1603256550859271 1101 3 0.23335003342928928 1101 4 0.057282289625767646 1101 5 0.10835251806749824 1101 6 0.051392719404340426 1101 7 0.27522311078156037 1101 8 0.18099005213674957 1101 9 0.22039574748018964 1101 10-11 0.17783213619895832 1101 12-13 0.2306911645919243 1101 14-15 0.1606431379725919 1101 16-17 0.2182714147614533 1101 18-19 0.33936209812851814 1101 20-21 0.42227491023698605 1101 22-23 0.28964251679018105 1101 24-25 0.17948584794108768 1101 26-27 -0.025058070115200337 1101 28-29 0.191816362793773 1101 30-31 0.19023155545490766 1101 32-33 -0.07596670137881745 1101 34-35 -0.23502149982201104 1101 36-37 -0.03386189447537902 1101 38-39 0.10411914198675021 1101 40-41 0.018412213596114668 1101 42-43 0.20103420665888194 1101 44-45 0.010962597225507409 1101 46-47 0.1768646102354836 1101 48-49 0.027942212008937872 1101 50-51 -0.049484925560001614 1101 52-53 0.06531429576019576 1101 54-55 0.2822736958694918 1101 56-57 0.105834279486416 1101 58-59 0.07536607289642205 1101 60-61 0.1936910978926747 1101 62-63 0.13376290083719056 1101 64-65 0.2889766247933281 1101 66-67 0.13851952830871284 1101 68-69 0.3339386099507209 1101 70-71 0.1816229556671516 1101 72-73 0.053446363444443534 1101 74-75 0.09008172019195726 1101 76-77 0.03528626452916939 1101 78-79 0.08777814318586152 1101 80-81 0.3401249987007624 1101 82-83 0.3479872182478978 1101 84-85 0.30846278528865767 1101 86-87 0.3729884649660633 1101 88-89 0.3590540238039388 1101 90-91 0.4410079617924261 1101 92-93 0.5742748131727033 1101 94-95 0.416861320144708 1101 96-97 0.5558983064094534 1101 98-99 0.7146702255525739 1101 100-101 1.2323926401561245 1102 1 0.08432292227762872 1102 2 -0.11556582269352589 1102 3 0.013366895602956674 1102 4 -0.06866255254720954 1102 5 -0.0795242613548055 1102 6 -0.06790959597678636 1102 7 -0.010069272486873615 1102 8 -0.001028827649591335 1102 9 -0.22019920458932773 1102 10-11 -0.0573732557978488 1102 12-13 0.08105643046356903 1102 14-15 -0.01113660110232928 1102 16-17 -0.11042644530200363 1102 18-19 -0.05076573274290297 1102 20-21 0.03310227764229978 1102 22-23 0.11157357681659619 1102 24-25 0.026702858267952934 1102 26-27 -0.19625299391864814 1102 28-29 -0.28989841305140374 1102 30-31 -0.11427688294211436 1102 32-33 -0.11001026036962003 1102 34-35 0.09077421445674361 1102 36-37 -0.2113898803228551 1102 38-39 -0.2848561154538132 1102 40-41 -0.07639365210105353 1102 42-43 -0.14682853719690314 1102 44-45 0.22124023827563377 1102 46-47 0.16101611637847668 1102 48-49 0.31094650552772407 1102 50-51 0.2363094241497734 1102 52-53 0.3145650011936141 1102 54-55 0.4488242117252952 1102 56-57 0.4388329685255101 1102 58-59 0.23082946589716613 1102 60-61 0.153699480244903 1102 62-63 0.34995204958982384 1102 64-65 0.32983328169682835 1102 66-67 0.39860149991593374 1102 68-69 0.5607421558806607 1102 70-71 0.7285592546637005 1102 72-73 0.1499676872712854 1102 74-75 0.4167985185232794 1102 76-77 0.3197233554630685 1102 78-79 0.22613325276562435 1102 80-81 0.1011475482029418 1102 82-83 0.017417949315724712 1102 84-85 0.023233757007968592 1102 86-87 -0.02227599337316022 1102 88-89 0.1450091977133816 1102 90-91 -0.11802426640248953 1102 92-93 -0.12600005000489034 1102 94-95 -0.10547618649538393 1102 96-97 0.04193340507266541 1102 98-99 -0.29992578893135047 1102 100-101 -0.1243251816019253 1103 1 0.1770460768225206 1103 2 0.11899174567023607 1103 3 0.13271680741452485 1103 4 -0.021887222609009882 1103 5 0.14099408798485058 1103 6 0.03385937399185934 1103 7 0.061143472040953384 1103 8 0.17307356549582664 1103 9 0.2393925279231226 1103 10-11 0.13523688516178112 1103 12-13 0.14380261329969102 1103 14-15 0.09052833962464746 1103 16-17 0.1203931632643318 1103 18-19 0.05362524697612514 1103 20-21 0.10628212055814856 1103 22-23 0.246208694251429 1103 24-25 0.2358536486537659 1103 26-27 0.17879842962695136 1103 28-29 0.24836070487519635 1103 30-31 0.22211585581681703 1103 32-33 0.04744036120509776 1103 34-35 0.1922736541174146 1103 36-37 0.20789524964277462 1103 38-39 0.40218136817340877 1103 40-41 0.19435427086056478 1103 42-43 0.20651860365396857 1103 44-45 0.16573827354155668 1103 46-47 0.4400343640531901 1103 48-49 0.22858844633508113 1103 50-51 0.4211883010122577 1103 52-53 0.473013357768032 1103 54-55 0.6174816197894515 1103 56-57 0.5848072733028218 1103 58-59 0.6790701895623954 1103 60-61 0.5151845449558152 1103 62-63 1.110417627732339 1103 64-65 1.1085559917531427 1103 66-67 1.0013445618748094 1103 68-69 1.3449027581153956 1103 70-71 1.4456777392413613 1103 72-73 1.2143452377609307 1103 74-75 1.1915302885808288 1103 76-77 1.1942640900040864 1103 78-79 1.1151931594230184 1103 80-81 1.2772832401628982 1103 82-83 1.3041863754951528 1103 84-85 1.3103071780095803 1103 86-87 1.4408314546476824 1103 88-89 1.3926224359072386 1103 90-91 1.3192130006547593 1103 92-93 1.4800412117752408 1103 94-95 1.4910156637592529 1103 96-97 1.502496865274388 1103 98-99 1.5750074195668908 1103 100-101 1.4272780508703278 1104 1 0.11781083351212374 1104 2 0.1635224206082171 1104 3 0.18486356377225377 1104 4 0.06556581510123038 1104 5 0.06050189340460577 1104 6 0.04783637257501283 1104 7 0.06781924396697292 1104 8 0.07440489056634902 1104 9 0.07579144039775088 1104 10-11 0.18447123496542162 1104 12-13 0.1600027417944645 1104 14-15 0.2667108532719027 1104 16-17 0.24271636593294232 1104 18-19 0.16351806871301733 1104 20-21 0.3000348063969156 1104 22-23 0.4211615724068807 1104 24-25 0.3431084980553223 1104 26-27 0.267625765828015 1104 28-29 0.27277258596221543 1104 30-31 0.2925412075819196 1104 32-33 0.2804945800461809 1104 34-35 0.33465395612270044 1104 36-37 0.3200516218351339 1104 38-39 0.22167811112853286 1104 40-41 0.2390657745473348 1104 42-43 0.04221547510386614 1104 44-45 0.009355444188578588 1104 46-47 0.018265135574999647 1104 48-49 0.008930446683976356 1104 50-51 0.022782146878085996 1104 52-53 -0.12891661683085687 1104 54-55 -0.30560446745935366 1104 56-57 -0.06092155165070068 1104 58-59 -0.17206094744622646 1104 60-61 -0.228613028960595 1104 62-63 -0.4173129927442858 1104 64-65 -0.4633968310899874 1104 66-67 -0.18979355299612877 1104 68-69 -0.4185817480519347 1104 70-71 -0.6708631982599655 1104 72-73 -0.4548700505651908 1104 74-75 -0.48136752402952965 1104 76-77 -0.18688674670116967 1104 78-79 0.018113650085876287 1104 80-81 -0.036218497920486925 1104 82-83 -0.09868731243271966 1104 84-85 -9.437740005999729E-4 1104 86-87 -0.12828980272906776 1104 88-89 -0.3474947411219773 1104 90-91 -0.5391449041144405 1104 92-93 -0.2857493927884498 1104 94-95 -0.07556335414917825 1104 96-97 0.11510424564021449 1104 98-99 0.43742865269131315 1104 100-101 0.4926593442426359 1105 1 0.05798964073898105 1105 2 0.09725422530287631 1105 3 0.014981163312420165 1105 4 0.05520012247566797 1105 5 0.010494902678964024 1105 6 0.06700261203067015 1105 7 -0.0034395526961219502 1105 8 0.08187719952535133 1105 9 0.07572541687784451 1105 10-11 -0.0636101992207827 1105 12-13 0.0756460439025588 1105 14-15 0.09732362921137394 1105 16-17 0.07390005113279585 1105 18-19 0.06522846625227885 1105 20-21 0.12456953476839061 1105 22-23 0.026494330947222977 1105 24-25 0.10520633714435235 1105 26-27 0.08325702835306714 1105 28-29 0.04055815710449906 1105 30-31 -0.0234959054037418 1105 32-33 0.1772668066699694 1105 34-35 0.22388008279465055 1105 36-37 0.14840406315187948 1105 38-39 0.0225647681635337 1105 40-41 0.21003096073366834 1105 42-43 0.16475318618494583 1105 44-45 0.09430441189142158 1105 46-47 -0.027630920102247103 1105 48-49 -0.04414275398092116 1105 50-51 0.26689634426511333 1105 52-53 0.28687426726290965 1105 54-55 0.3357887841601652 1105 56-57 0.5083371207328327 1105 58-59 0.6290529081719853 1105 60-61 0.6843446693818294 1105 62-63 0.8271818108163203 1105 64-65 0.8306835777897774 1105 66-67 0.6383180966597735 1105 68-69 0.8725159512225815 1105 70-71 0.9281371279738977 1105 72-73 1.0824079938958207 1105 74-75 0.9187990882648336 1105 76-77 0.7306537606701617 1105 78-79 1.0895549823628379 1105 80-81 1.4382964233950126 1105 82-83 1.2514781347406974 1105 84-85 1.3227559510308602 1105 86-87 1.2883108404176902 1105 88-89 0.9667966323225876 1105 90-91 1.026478590937682 1105 92-93 1.073422439247711 1105 94-95 0.999526748536816 1105 96-97 1.3692326575026996 1105 98-99 1.1850343291605334 1105 100-101 1.060350928962464 1106 1 -0.018610580744962135 1106 2 0.03354436926743887 1106 3 -0.05223921321028513 1106 4 -0.09668249103485493 1106 5 0.03698438219170441 1106 6 -0.029907686972649117 1106 7 -0.03940854494418744 1106 8 -0.07391460556878116 1106 9 -0.04811732952303771 1106 10-11 -0.06709857131380659 1106 12-13 -0.06344033483497924 1106 14-15 -0.11821900644753924 1106 16-17 -0.0812992844153726 1106 18-19 -0.12221339421283517 1106 20-21 -0.20053013300569944 1106 22-23 -0.05921441766850677 1106 24-25 0.07205019096495135 1106 26-27 -0.07198106688503003 1106 28-29 0.0027010142473571364 1106 30-31 -0.05899424427416733 1106 32-33 -0.12610528635328677 1106 34-35 -0.41768691609793507 1106 36-37 -0.22950845069086512 1106 38-39 -0.17297786749538346 1106 40-41 -0.14525143129291251 1106 42-43 -0.4520740563399741 1106 44-45 0.08853475519153164 1106 46-47 -0.14506724346880162 1106 48-49 -0.5326975712566693 1106 50-51 -0.1851689934979035 1106 52-53 -0.04423868954771848 1106 54-55 -0.40465972082322565 1106 56-57 -0.322526666642581 1106 58-59 -0.20715971641273256 1106 60-61 -0.1936841235306872 1106 62-63 -0.11735307290460639 1106 64-65 -0.326603243915649 1106 66-67 -0.6359620805273778 1106 68-69 -0.6143389767951319 1106 70-71 -0.3481530159906612 1106 72-73 -0.01345579347959358 1106 74-75 -0.15737477662996469 1106 76-77 -0.14719784508841727 1106 78-79 -0.45561666769253506 1106 80-81 -0.6650036873469602 1106 82-83 -0.5179792296003853 1106 84-85 -0.20091514531465293 1106 86-87 -0.3198176202689069 1106 88-89 -0.4054182071015511 1106 90-91 -0.6174748974344126 1106 92-93 -0.6422032528896082 1106 94-95 -0.6088508815849991 1106 96-97 -0.6116311298727162 1106 98-99 -0.3792236996323801 1106 100-101 -0.27348628033986344 1107 1 0.16150329089064286 1107 2 0.10487977299415263 1107 3 0.09258480335286379 1107 4 0.05347278846824821 1107 5 0.0641686472020524 1107 6 0.06245256147455791 1107 7 -0.022524486973168223 1107 8 0.00368373811652134 1107 9 0.07741062078751781 1107 10-11 0.20210432723691696 1107 12-13 0.16064351609669103 1107 14-15 0.31174476166840037 1107 16-17 0.31046054995315586 1107 18-19 0.06499675071469824 1107 20-21 0.07074833490320742 1107 22-23 0.07995742498160041 1107 24-25 0.16928621580967018 1107 26-27 0.20553963704872302 1107 28-29 0.2063190266697177 1107 30-31 0.2409125880239671 1107 32-33 0.11733674263221872 1107 34-35 0.3066867899062089 1107 36-37 0.35562094889505147 1107 38-39 0.3692322774321539 1107 40-41 0.36656533889343024 1107 42-43 0.30454085049232305 1107 44-45 0.25623144256213237 1107 46-47 0.5627381395539715 1107 48-49 0.9106305860932267 1107 50-51 1.1295100955290067 1107 52-53 0.9257561344688483 1107 54-55 0.903828892013216 1107 56-57 0.7351234368770818 1107 58-59 0.6298744450779523 1107 60-61 0.8385829572146548 1107 62-63 0.7646396807185809 1107 64-65 0.680679364780012 1107 66-67 0.6899695964912524 1107 68-69 0.5140098844885159 1107 70-71 0.42775795709422226 1107 72-73 0.37078851293862414 1107 74-75 0.4532825732665131 1107 76-77 0.45168931847265625 1107 78-79 0.5164381977318975 1107 80-81 0.5280967267317145 1107 82-83 0.42111497329816316 1107 84-85 0.5071593888468371 1107 86-87 0.6269525660664961 1107 88-89 0.4982256852379905 1107 90-91 0.4462207547394996 1107 92-93 0.5465855669861703 1107 94-95 0.21493793829077745 1107 96-97 0.4628667997339093 1107 98-99 0.4632193645498113 1107 100-101 0.6807166182108588 1108 1 -0.014299264820742508 1108 2 -0.031340423359289105 1108 3 0.022107945007846297 1108 4 4.0499236526159166E-5 1108 5 -0.04643599671254606 1108 6 0.01605237447127905 1108 7 0.0506989819375363 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-0.736569920058848 2316 92-93 -0.8310375558415579 2316 94-95 -0.728484581218698 2316 96-97 -0.580853280271338 2316 98-99 -0.5439942232264308 2316 100-101 -0.9165028715917458 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 0.0 4 6.0 5 14.0 6 35.0 7 63.0 8 156.0 9 308.0 10 562.0 11 864.0 12 1255.0 13 1997.0 14 2482.0 15 3575.0 16 4745.0 17 6816.0 18 6640.0 19 4389.0 20 3565.0 21 3444.0 22 3626.0 23 4014.0 24 4304.0 25 4975.0 26 5130.0 27 5041.0 28 5910.0 29 7196.0 30 8782.0 31 10536.0 32 13431.0 33 17038.0 34 23932.0 35 37555.0 36 55195.0 37 108839.0 38 147490.0 39 23128.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.17717373567951 21.329419666743828 15.599515788115559 21.893890809461105 2 15.206104378650615 22.357419643772083 40.62747881267482 21.80899716490248 3 18.217405081905934 26.34670886724465 31.975286855772318 23.460599195077105 4 12.125978574762543 20.227609943799546 37.38203027462707 30.264381206810842 5 12.139449986908064 38.51173151285856 35.5398620982768 13.808956401956578 6 28.62750976203035 38.515905753241675 18.05909206476903 14.79749241995894 7 24.89004671354465 35.29737667965741 21.659564133408722 18.153012473389218 8 23.83799362857918 36.01939887029662 21.23819588988333 18.90441161124087 9 24.521478445658772 17.518025197328477 21.843313600485732 36.117182756527015 10-11 22.774845268498524 28.359789162913014 28.52875102932973 20.33661453925873 12-13 24.286584370885052 26.46145847958986 28.378668113736666 20.87328903578842 14-15 21.462805620804414 28.292147494886557 27.580724116863554 22.66432276744548 16-17 19.520189586588533 31.270213066712966 28.698214466982204 20.511382879716304 18-19 19.12304901696639 29.374129576011022 31.76881538852691 19.734006018495677 20-21 20.50661996577123 28.356279006227208 31.859130771361677 19.277970256639886 22-23 19.894714273245775 29.153843526701856 31.367044751651672 19.584397448400697 24-25 19.683136641749343 29.159626154746736 31.91118945673655 19.246047746767374 26-27 19.855707688030265 29.16078733537477 31.894996645578573 19.088508331016392 28-29 19.526925299051403 29.30623110324329 31.96361197363016 19.203231624075144 30-31 19.78305334299733 29.43228433407584 32.04165891902353 18.743003403903295 32-33 19.636365361262904 29.374810261080754 31.77491272009715 19.213911657559198 34-35 19.835099715424125 29.35762144292019 31.82780455264464 18.979474289011044 36-37 19.431755329191226 29.43865251557559 31.59262390031621 19.53696825491697 38-39 19.29355948286087 28.848297948048867 32.14109832760052 19.717044241489738 40-41 19.52274816895956 28.71082797471129 32.13626603435401 19.630157821975143 42-43 19.592104713869883 28.875346775509588 31.88778705042524 19.644761460195294 44-45 19.589188860986223 29.00562680907462 31.592194379642162 19.812989950296995 46-47 19.84282087575563 28.69486682275796 30.91129359709473 20.55101870439168 48-49 19.708106754300417 28.562239821494302 31.142204985560923 20.58744843864436 50-51 19.744821095851943 28.838403770477495 30.88046113971942 20.536313993951147 52-53 19.78988391816971 29.023493383828992 30.960530659795616 20.226092038205685 54-55 19.795386325947458 28.895135492048073 30.53333510422319 20.776143077781278 56-57 20.08843697466236 29.169686666337785 29.94865684328763 20.79321951571222 58-59 19.565423628477426 29.490249353941433 30.312954185814412 20.63137283176673 60-61 19.810375643686843 29.79060492332679 29.6937435726185 20.705275860367863 62-63 19.502145939033323 29.882817688153885 29.86441308282831 20.75062328998448 64-65 19.658110738802602 29.76764660121964 29.670121166814027 20.904121493163732 66-67 19.563715984684332 29.808535183154284 29.589956777638214 21.03779205452317 68-69 19.743928597517648 29.6385192409312 29.47467983959487 21.142872321956283 70-71 19.65393649841948 29.211903415667063 29.573070077906504 21.561090008006953 72-73 19.606786555910155 29.075102174020284 29.62429939169933 21.693811878370227 74-75 19.886176054280305 28.9666667931588 29.37185271762023 21.77530443494067 76-77 19.675946129530473 28.888304916875697 29.709207235855967 21.726541717737867 78-79 19.525673475738177 28.687941378485966 29.7749515218901 22.01143362388576 80-81 19.52738111953127 28.798179272240166 29.562539607849093 22.111900000379475 82-83 19.730780469108726 28.681680017911287 29.627999286584373 21.959540226395617 84-85 19.908240169402763 28.497347389512512 29.349579149494904 22.24483329158982 86-87 19.762219637561405 28.437825520215693 29.376746780610997 22.423208061611906 88-89 19.96114157993251 28.260013680137142 29.321884519530244 22.456960220400102 90-91 20.036543577172218 28.082012439236344 29.326884764402077 22.554559219189365 92-93 19.807908135917707 27.984904357263545 29.5799460951219 22.627241411696847 94-95 19.77394590943416 28.19822708626637 29.36084790206473 22.666979102234734 96-97 19.9314286147973 28.089127621707565 29.182209387487145 22.797234376007985 98-99 19.902208932115467 27.890566596210547 29.220157027333688 22.9870674443403 100-101 19.987401383570948 27.925763032168216 29.173576299422056 22.91325928483878 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3876.0 1 2208.5 2 603.5 3 707.5 4 521.5 5 380.0 6 733.5 7 1445.0 8 2018.5 9 2168.0 10 1621.5 11 951.5 12 864.0 13 861.0 14 863.5 15 883.0 16 878.0 17 949.5 18 1089.0 19 1180.0 20 1288.5 21 1487.5 22 1737.0 23 2000.5 24 2409.5 25 3015.0 26 3726.0 27 4626.0 28 5684.0 29 6778.5 30 7886.0 31 9169.5 32 10634.5 33 12148.0 34 13604.0 35 14981.5 36 16186.5 37 17213.0 38 17982.5 39 18590.0 40 19203.5 41 19753.0 42 20285.5 43 20750.0 44 21134.0 45 21531.5 46 21498.5 47 21402.0 48 22321.5 49 21705.0 50 20017.5 51 18440.0 52 16020.0 53 13740.0 54 11829.5 55 9902.5 56 8076.0 57 6583.5 58 5121.0 59 3923.0 60 2991.5 61 2185.0 62 1507.0 63 980.0 64 603.5 65 406.0 66 269.5 67 197.0 68 155.5 69 116.5 70 95.0 71 64.0 72 48.0 73 40.5 74 31.0 75 26.5 76 22.0 77 15.0 78 9.0 79 6.0 80 5.5 81 6.5 82 5.5 83 3.5 84 1.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.014420103141685103 3 0.0066408369731444546 4 0.0 5 0.0 6 0.0 7 0.0 8 1.8973819923269873E-4 9 3.7947639846539746E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 9.486909961634937E-5 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.006166491475062709 26-27 0.023812144003703687 28-29 9.486909961634937E-5 30-31 0.0 32-33 3.7947639846539746E-4 34-35 0.0222942384098421 36-37 0.00294094208810683 38-39 0.019827641819817016 40-41 0.016602092432861138 42-43 0.008348480766238744 44-45 0.0021819892911760356 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.846072988490481E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.002656334789257782 86-87 1.8973819923269873E-4 88-89 9.486909961634937E-5 90-91 0.0 92-93 4.7434549808174684E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 527042.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.48199416078418 #Duplication Level Percentage of deduplicated Percentage of total 1 79.09908981656109 53.37764317124519 2 13.409756248973176 18.098341857814944 3 4.04932399193939 8.197693739375318 4 1.5984490623808258 4.314661211755754 5 0.7336566687428527 2.475430751806279 6 0.3645740720279693 1.4761311239858845 7 0.2228096257977968 1.0524946506937405 8 0.12930901443527043 0.6980824125646142 9 0.08811359238452882 0.5351472834100643 >10 0.27796534926541167 2.9466288451334286 >50 0.01375703622556264 0.665761136113255 >100 0.00982645200672187 1.3076501137232694 >500 0.0011230230864823047 0.5566322854416996 >1k 0.0022460461729646095 4.297701416936526 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4745 0.9003077553591554 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4457 0.8456631539801381 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3922 0.7441532173906443 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2820 0.5350617218362104 No Hit CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT 2627 0.49844224938429954 No Hit ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT 1598 0.30320164237385255 TruSeq Adapter, Index 3 (95% over 21bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1442 0.2736024832935515 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1073 0.20358908777668572 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 926 0.17569757248947904 No Hit ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT 857 0.16260563674242282 RNA PCR Primer, Index 3 (95% over 23bp) GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 595 0.11289422854345575 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 560 0.10625339157031127 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 6.640836973144455E-4 0.0 22-23 0.0 0.0 0.0 0.0027512038888741317 0.0 24-25 0.0 0.0 0.0 0.008633088065087792 0.0 26-27 0.0 0.0 0.0 0.02087120191559686 0.0 28-29 0.0 0.0 0.0 0.04752941890779103 0.0 30-31 0.0 0.0 0.0 0.11735307622542415 0.0 32-33 0.0 0.0 0.0 0.21990657291069782 0.0 34-35 0.0 0.0 0.0 0.39617335999787495 0.0 36-37 0.0 0.0 0.0 0.6314487270464213 0.0 38-39 0.0 0.0 0.0 0.8963232531752687 0.0 40-41 0.0 0.0 0.0 1.1513313929440159 0.0 42-43 0.0 0.0 0.0 1.3843299016017698 0.0 44-45 0.0 0.0 0.0 1.6742498700293336 0.0 46-47 0.0 0.0 0.0 2.0006944418091916 0.0 48-49 0.0 0.0 0.0 2.3172726272289497 0.0 50-51 0.0 0.0 0.0 2.599508198587589 0.0 52-53 0.0 0.0 0.0 2.865900630310298 0.0 54-55 0.0 0.0 0.0 3.1674894979906725 0.0 56-57 0.0 0.0 0.0 3.5274228619351016 0.0 58-59 0.0 0.0 0.0 3.8892536078718583 0.0 60-61 0.0 0.0 0.0 4.244822993233935 0.0 62-63 0.0 0.0 0.0 4.587964526546271 0.0 64-65 0.0 0.0 0.0 4.951408047176505 0.0 66-67 0.0 0.0 0.0 5.379172817346625 0.0 68-69 0.0 0.0 0.0 5.8353983174016495 0.0 70-71 0.0 0.0 0.0 6.296367272437491 0.0 72-73 0.0 0.0 0.0 6.755438845481005 0.0 74-75 0.0 0.0 0.0 7.221246124597281 0.0 76-77 0.0 0.0 0.0 7.713521882506518 0.0 78-79 0.0 0.0 0.0 8.245263185856157 0.0 80-81 0.0 0.0 0.0 8.788104173860907 0.0 82-83 0.0 0.0 0.0 9.334834794949929 0.0 84-85 0.0 0.0 0.0 9.91296708801196 0.0 86-87 0.0 0.0 0.0 10.507416866208008 0.0 88-89 0.0 0.0 0.0 11.13156067258397 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2880 0.0 36.28849 1 GGCGGGT 30 0.0039487956 31.669958 24-25 TTGCGCA 65 0.0058436915 29.228258 4 GTATCAA 4015 0.0 28.278154 1 TTGCCGT 45 8.946583E-4 26.389128 90-91 TATCAAC 4655 0.0 24.186186 2 TCAACGC 4680 0.0 23.950933 4 ATCAACG 4690 0.0 23.899866 3 CAGCGTG 50 0.001652555 23.752468 80-81 CAACGCA 4740 0.0 23.547556 5 AACGCAG 4815 0.0 23.180769 6 GAGTACT 3320 0.0 22.961132 12-13 GTACTGC 105 0.0021957513 22.617104 6 GTCCTAC 170 3.140789E-6 22.355263 1 GTACTTT 3375 0.0 22.024035 14-15 GTACATG 2230 0.0 20.663582 1 CGCAGAA 115 0.003722494 20.6504 9 GTAGGCT 255 1.1892553E-8 20.488436 4 ACGCAGA 5380 0.0 20.393229 7 GTCTAAG 140 4.843548E-4 20.359259 1 >>END_MODULE