FastQCFastQC Report
Thu 26 May 2016
SRR938096_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938096_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences510478
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43950.860957768992983No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40860.8004262671456949No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38640.7569376153330799No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25490.49933591653313164No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14620.28639823851370677No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9890.19373998487691926No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8480.1661188141310693No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5780.1132272105751864No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5560.10891752435952186No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCAT250.004990099557.0851636
GTATAGG1054.548565E-836.280181
GGTATCA26000.036.2627331
GTATCAA37550.030.4347721
ACGCAGT650.00577081429.3032231
TATCAAC42850.026.6441822
ATCAACG42650.026.434513
TCAACGC42900.026.1695774
CAACGCA43350.025.8979195
AACGCAG43950.025.4361256
GAGTACT31000.023.61347412-13
GTACTTT31650.022.75194214-15
CGCAGAG47950.022.7011418
ACGCAGA48550.022.6186267
GTACATG19100.021.6897541
CGCCGTC550.002876801221.59135494-95
GCAGAGT51050.021.5088449
GTCCTAT1553.8271482E-521.5047861
TACATGG19500.020.7360632
AGTACTT30400.020.63959712-13
Produced by FastQC (version 0.11.5)