FastQCFastQC Report
Thu 26 May 2016
SRR938096_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938096_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences510478
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44710.8758457759198242No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43720.8564521879493338No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37530.7351932894267725No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26720.5234309803752561No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT26450.5181418200196679No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT16610.32538130928267234TruSeq Adapter, Index 3 (95% over 21bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13170.2579934884559178No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10250.20079219868437032No Hit
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT8820.17277923828255087RNA PCR Primer, Index 3 (95% over 23bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8440.16533523481913032No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6180.12106300369457644No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5810.11381489505914065No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27900.033.537521
TACGAAA650.005842688529.2291132
GTATCAA38400.025.7277071
CCTATAC1151.2808823E-424.7812043
CTATACT1351.3414159E-524.6282354
GTACATG21800.023.3128971
TATCAAC44150.022.3769862
TACATGG22800.022.2904032
ATCAACG44000.022.1294273
GTGTAGC3450.022.0277351
TATACCA1302.9395358E-421.9218335
TCAACGC44450.021.9053944
CAACGCA45150.021.5657775
AACGCAG46200.021.0756446
ACATGGG22750.020.8779373
GAGTACT31450.020.53937512-13
CATGGGG8800.019.9704574
GTGTTAT2151.0769727E-619.8825931
GTACTTT32900.019.63414614-15
GTATTAG1707.946271E-519.5577141
Produced by FastQC (version 0.11.5)