##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938096_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 510478 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.51991466821293 34.0 31.0 34.0 31.0 34.0 2 32.72194100431361 34.0 31.0 34.0 31.0 34.0 3 32.76800175521766 34.0 31.0 34.0 31.0 34.0 4 36.23010590074401 37.0 35.0 37.0 35.0 37.0 5 36.20841250749298 37.0 37.0 37.0 35.0 37.0 6 36.245324969930145 37.0 37.0 37.0 35.0 37.0 7 36.20263361006743 37.0 37.0 37.0 35.0 37.0 8 36.201585572737706 37.0 37.0 37.0 35.0 37.0 9 37.8949298500621 39.0 38.0 39.0 35.0 39.0 10-11 37.883654143763295 39.0 38.0 39.0 35.0 39.0 12-13 37.822402728423164 39.0 38.0 39.0 35.0 39.0 14-15 39.09541253491825 40.0 38.5 41.0 36.0 41.0 16-17 38.99789608954744 40.0 38.0 41.0 36.0 41.0 18-19 39.222086945960456 40.5 39.0 41.0 36.0 41.0 20-21 39.22259431356493 41.0 39.0 41.0 36.0 41.0 22-23 39.11875144472435 40.5 39.0 41.0 35.0 41.0 24-25 39.02838026320429 40.5 38.5 41.0 35.0 41.0 26-27 38.86329283534256 40.0 38.0 41.0 35.0 41.0 28-29 38.731593721962554 40.0 38.0 41.0 35.0 41.0 30-31 38.452940185473224 40.0 38.0 41.0 34.5 41.0 32-33 38.205683692539154 40.0 37.0 41.0 34.0 41.0 34-35 38.032048393858304 40.0 37.0 41.0 34.0 41.0 36-37 37.986850559671524 40.0 37.0 41.0 34.0 41.0 38-39 37.830326674215144 40.0 37.0 41.0 33.0 41.0 40-41 37.658129243571715 40.0 37.0 41.0 33.0 41.0 42-43 37.41440865228276 40.0 37.0 41.0 33.0 41.0 44-45 37.08466672412915 40.0 36.5 41.0 32.5 41.0 46-47 36.619012964319715 40.0 36.0 41.0 31.0 41.0 48-49 36.19564016470837 40.0 36.0 41.0 30.0 41.0 50-51 35.81783935840526 39.5 35.0 41.0 28.5 41.0 52-53 35.368392957189144 39.0 35.0 40.5 27.0 41.0 54-55 35.123134591500516 39.0 35.0 40.0 26.0 41.0 56-57 34.79800696602008 38.5 35.0 40.0 24.5 41.0 58-59 34.39231661305678 38.0 34.0 40.0 23.0 41.0 60-61 33.958106911561316 38.0 34.0 40.0 21.5 41.0 62-63 33.52434091185124 38.0 33.5 40.0 18.5 41.0 64-65 33.06285481450719 37.0 33.0 40.0 13.0 41.0 66-67 32.52405392592824 36.5 32.5 39.0 7.5 41.0 68-69 31.99113771798197 36.0 31.5 39.0 2.0 40.0 70-71 31.483630048699453 35.5 31.0 39.0 2.0 40.0 72-73 30.974492534448107 35.0 31.0 38.0 2.0 40.0 74-75 30.438637904082057 35.0 30.0 37.0 2.0 39.0 76-77 29.019615928600253 33.5 28.5 35.5 2.0 38.5 78-79 29.690559632344588 34.0 30.0 36.0 2.0 39.0 80-81 29.392535035789983 34.0 29.0 36.0 2.0 37.5 82-83 28.895777095193132 34.0 29.0 35.5 2.0 37.0 84-85 28.384676714765376 34.0 28.5 35.0 2.0 37.0 86-87 27.831879140726926 34.0 27.0 35.0 2.0 36.0 88-89 27.215757584068264 33.0 26.0 35.0 2.0 36.0 90-91 26.792562069276247 33.0 25.5 35.0 2.0 35.0 92-93 26.134042994996847 32.5 24.5 35.0 2.0 35.0 94-95 25.49123370644768 32.0 21.5 34.0 2.0 35.0 96-97 24.644657556251197 32.0 12.5 34.0 2.0 35.0 98-99 23.420270805010205 31.0 2.0 34.0 2.0 35.0 100-101 19.141461532132627 25.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2646695114786013 1101 2 0.23674610378695604 1101 3 0.2441297744981057 1101 4 0.09861680870297107 1101 5 0.21562680729962125 1101 6 0.11934127646958359 1101 7 0.21803037321132024 1101 8 0.15646483092952934 1101 9 0.12037449712434523 1101 10-11 0.2935807081795403 1101 12-13 0.21792205677741094 1101 14-15 0.2738509169211554 1101 16-17 0.237200607346729 1101 18-19 0.3902593437550266 1101 20-21 0.5853023931267529 1101 22-23 0.5168827755504495 1101 24-25 0.4531690655456728 1101 26-27 0.5848444009133189 1101 28-29 0.5494359208602475 1101 30-31 0.6012518861760512 1101 32-33 0.4767943881867396 1101 34-35 0.2799735166242314 1101 36-37 0.394698360120735 1101 38-39 0.522358433755663 1101 40-41 0.5874574608969994 1101 42-43 0.5257458183554888 1101 44-45 0.5165299749323182 1101 46-47 0.22366116957110904 1101 48-49 0.34394256566189796 1101 50-51 0.5272341923215791 1101 52-53 0.30082586704381953 1101 54-55 0.4656423880307443 1101 56-57 0.663719991150792 1101 58-59 0.5480754376236163 1101 60-61 0.4064680550138604 1101 62-63 0.15528603232427685 1101 64-65 0.18520035643164334 1101 66-67 0.057182835936835374 1101 68-69 0.3069611633705662 1101 70-71 0.565029594866644 1101 72-73 0.21980798629782328 1101 74-75 0.43418806928182363 1101 76-77 0.11120062057947777 1101 78-79 0.3572910036184176 1101 80-81 0.38218903747474187 1101 82-83 0.4539829145527996 1101 84-85 0.08469987154631653 1101 86-87 0.021207114653680748 1101 88-89 0.1604042449487899 1101 90-91 -0.024697110422920332 1101 92-93 0.36089132270345203 1101 94-95 0.28115089071357247 1101 96-97 -0.22512713673618023 1101 98-99 0.19818009037446416 1101 100-101 -0.4205379627699841 1102 1 0.25781071716102133 1102 2 0.25143043642830065 1102 3 0.20945419343407679 1102 4 0.16325127770610948 1102 5 0.26821259310985823 1102 6 0.2374010721304316 1102 7 0.2951205755152202 1102 8 0.1954500550106104 1102 9 0.39904100097096773 1102 10-11 0.33611490038859415 1102 12-13 0.30536914026338735 1102 14-15 0.3618119011683163 1102 16-17 0.34736617497809874 1102 18-19 0.4246906947543394 1102 20-21 0.3885114744146634 1102 22-23 0.3785925895285587 1102 24-25 0.27194526939636887 1102 26-27 0.3173488784645073 1102 28-29 0.41203106882296936 1102 30-31 0.31004315088073753 1102 32-33 0.13059827144476088 1102 34-35 0.31063202193623596 1102 36-37 0.5811680145352014 1102 38-39 0.16952994431861867 1102 40-41 0.24125880009185607 1102 42-43 0.07942555133062257 1102 44-45 -0.04411540301369854 1102 46-47 0.1264906766334306 1102 48-49 0.16468787440940957 1102 50-51 0.5065000774520385 1102 52-53 0.36581396082815587 1102 54-55 0.508161315860761 1102 56-57 0.5498757081695587 1102 58-59 0.6127378913216646 1102 60-61 0.6974071196127127 1102 62-63 0.7731270385521398 1102 64-65 0.7894051840459184 1102 66-67 0.2378927313249548 1102 68-69 0.6335450971028038 1102 70-71 0.45828579220453136 1102 72-73 0.6184143484910081 1102 74-75 0.42710285334174003 1102 76-77 0.3833619548897289 1102 78-79 0.32024435567605636 1102 80-81 0.13904274366597136 1102 82-83 0.19190624666498124 1102 84-85 0.19159631799887222 1102 86-87 0.16535627816570653 1102 88-89 -0.15440067628701115 1102 90-91 -0.2745037616216841 1102 92-93 -0.020835180329068947 1102 94-95 0.07932018285344 1102 96-97 -0.19860527814538642 1102 98-99 0.34821621607154185 1102 100-101 -0.23271486674686415 1103 1 -0.007665340848696189 1103 2 0.07008515991778808 1103 3 0.040399818596341674 1103 4 0.13809920619540605 1103 5 -0.030705288274369025 1103 6 -0.13519731071887264 1103 7 -0.16510437904877762 1103 8 -0.15959055920992427 1103 9 0.01771241523601219 1103 10-11 0.09046145652524018 1103 12-13 0.006022398673685814 1103 14-15 0.15415778967393834 1103 16-17 0.048988425636466104 1103 18-19 0.031099025305870498 1103 20-21 0.11101573983508928 1103 22-23 0.14411037447718655 1103 24-25 0.2225730280106646 1103 26-27 0.14483812218691838 1103 28-29 0.08249488693060414 1103 30-31 -0.08024745406715539 1103 32-33 0.09515819132904113 1103 34-35 0.2955060065807231 1103 36-37 0.04038688623877107 1103 38-39 0.14262302321035492 1103 40-41 0.1860285601155951 1103 42-43 0.29239057488315723 1103 44-45 0.23610880973804882 1103 46-47 0.27516144181276303 1103 48-49 0.1348021134954962 1103 50-51 0.3340333662766355 1103 52-53 0.095722439742147 1103 54-55 0.08139904814071741 1103 56-57 0.07417701375911889 1103 58-59 0.1572860349365115 1103 60-61 0.03222407744068789 1103 62-63 0.03261296266475 1103 64-65 -0.09613318619257427 1103 66-67 -0.09406824935083336 1103 68-69 0.19438065587288378 1103 70-71 -0.08011690057392684 1103 72-73 -0.1987451596874692 1103 74-75 0.017638719963898097 1103 76-77 -0.1500311870560438 1103 78-79 -0.08499376605533371 1103 80-81 0.03565729802226514 1103 82-83 -0.10451960920090286 1103 84-85 -0.3500332580554293 1103 86-87 -0.10164744944083637 1103 88-89 -0.4610032689208161 1103 90-91 -0.7329251851593845 1103 92-93 -0.7923709171340825 1103 94-95 -0.6741524257293285 1103 96-97 -0.5711781611986844 1103 98-99 -0.5313343384331155 1103 100-101 -1.0992535708008688 1104 1 0.16471974230118747 1104 2 0.14912441363937035 1104 3 0.05082243362217298 1104 4 0.13899282228921805 1104 5 0.09644102501435725 1104 6 0.1341952482924853 1104 7 -0.011720625425269304 1104 8 0.016421297549953806 1104 9 0.17809688811149726 1104 10-11 0.2904353019078556 1104 12-13 0.14470943688122162 1104 14-15 0.21331081598112434 1104 16-17 0.2330123135203408 1104 18-19 0.2650913096937515 1104 20-21 0.21975356159136794 1104 22-23 0.2575625660161691 1104 24-25 0.20084290007099526 1104 26-27 0.3665508137150013 1104 28-29 0.07925389392696047 1104 30-31 0.2446561007812562 1104 32-33 0.16733185798844374 1104 34-35 0.41815372568012776 1104 36-37 0.5058017638569581 1104 38-39 0.5339832812692364 1104 40-41 0.3515567744090973 1104 42-43 0.39661672514143476 1104 44-45 0.5703582811968246 1104 46-47 0.7997249866352334 1104 48-49 0.802172048762813 1104 50-51 0.9201779577197158 1104 52-53 0.7893014811754213 1104 54-55 0.701806188351263 1104 56-57 0.40018996029471765 1104 58-59 0.32566292297295263 1104 60-61 0.4193821603072365 1104 62-63 0.48924206839479467 1104 64-65 0.5735252780954738 1104 66-67 0.25559936905330005 1104 68-69 0.5170479909353247 1104 70-71 0.776991706474746 1104 72-73 0.36935710486648077 1104 74-75 0.41938672022544665 1104 76-77 0.49336150272839774 1104 78-79 0.6813539595827294 1104 80-81 0.6302934219079681 1104 82-83 0.4365910902171599 1104 84-85 0.41263538473788586 1104 86-87 0.4645084756697777 1104 88-89 0.3218676362469033 1104 90-91 -0.02983022210278108 1104 92-93 -0.1178825668365775 1104 94-95 0.12305774841683004 1104 96-97 -0.10219317830816621 1104 98-99 0.11628232205815436 1104 100-101 -0.6216550005866175 1105 1 0.12491943383459159 1105 2 0.16025762250372821 1105 3 0.15062355384296922 1105 4 0.015423539068173397 1105 5 0.13109075307669826 1105 6 0.20157084696120364 1105 7 0.1975414900061736 1105 8 0.13007500320787813 1105 9 0.13640113358597006 1105 10-11 0.19471933470273228 1105 12-13 0.22677575719942666 1105 14-15 0.20010966329015645 1105 16-17 0.06929772629734288 1105 18-19 0.1989171072422522 1105 20-21 0.08063918542446658 1105 22-23 0.07198946755038094 1105 24-25 0.22095411039650514 1105 26-27 0.14411189821861825 1105 28-29 0.1408092149757536 1105 30-31 0.21531321580682317 1105 32-33 0.2877677699942893 1105 34-35 0.19575945666478134 1105 36-37 0.37846391175857264 1105 38-39 0.31771166831364184 1105 40-41 0.399093100409587 1105 42-43 0.5058483997903522 1105 44-45 0.6518543441889477 1105 46-47 0.5274524807500214 1105 48-49 0.5892976271376824 1105 50-51 0.5417604725342287 1105 52-53 0.48581499688285845 1105 54-55 0.7506758206266113 1105 56-57 0.7429876711478656 1105 58-59 0.8778729237847145 1105 60-61 0.9265824200685842 1105 62-63 1.0326587418260758 1105 64-65 0.880975152273578 1105 66-67 0.9654966821468989 1105 68-69 0.9172773114970596 1105 70-71 0.6837312957271138 1105 72-73 0.8306242531739763 1105 74-75 0.40259152581031543 1105 76-77 0.32160446076071025 1105 78-79 0.7707727435749376 1105 80-81 0.7063344966705323 1105 82-83 0.8614997678695673 1105 84-85 0.6427981414551525 1105 86-87 0.5728106917587432 1105 88-89 0.7938742926313473 1105 90-91 0.3974122488770071 1105 92-93 0.27036526172094 1105 94-95 0.2665698029667105 1105 96-97 0.5558756584480662 1105 98-99 0.35314610970325333 1105 100-101 -1.2467706755602386 1106 1 0.14217379877810288 1106 2 0.1341535485564478 1106 3 0.08990804545543085 1106 4 0.04015821897231575 1106 5 0.029139366565232194 1106 6 0.054925862366886236 1106 7 0.12546001106061055 1106 8 0.05277270947285473 1106 9 -0.027618037930636774 1106 10-11 0.16223046102933125 1106 12-13 0.2138264247790218 1106 14-15 0.10543318263284363 1106 16-17 0.17140316963867264 1106 18-19 0.0969828381563147 1106 20-21 0.30474907929643535 1106 22-23 0.19468717381536038 1106 24-25 0.0961252846518974 1106 26-27 0.20572607144696065 1106 28-29 0.23121489796103134 1106 30-31 0.3552567283025496 1106 32-33 0.36435113185666523 1106 34-35 0.22353247960213452 1106 36-37 0.18894833490133323 1106 38-39 0.18981026468474482 1106 40-41 0.35148953998506727 1106 42-43 0.010298930430543862 1106 44-45 0.11166262902290924 1106 46-47 -0.3617720998730576 1106 48-49 -0.07240213321836109 1106 50-51 0.017522197970727404 1106 52-53 -0.07683478059060889 1106 54-55 -0.19598628139323893 1106 56-57 -0.24693016520954103 1106 58-59 -0.07009010257612402 1106 60-61 -0.1868034067030777 1106 62-63 -0.21485923147074004 1106 64-65 -0.3358427183186876 1106 66-67 -0.4247121504446838 1106 68-69 -0.6007131027446384 1106 70-71 -0.8589338874293375 1106 72-73 -0.7046119672984616 1106 74-75 -0.6406984433783194 1106 76-77 -0.6415331633406254 1106 78-79 -0.47300507224156263 1106 80-81 -0.43475588333631876 1106 82-83 -0.5523824642427222 1106 84-85 -0.3624654668981542 1106 86-87 -0.37929475158257375 1106 88-89 -0.4583766488270591 1106 90-91 -0.6085617326361721 1106 92-93 -0.2546261795662943 1106 94-95 -0.5887194815249011 1106 96-97 -0.5315858273444682 1106 98-99 -1.0803783682152641 1106 100-101 -2.128557734786529 1107 1 0.011858681107582925 1107 2 -0.0051364394770558874 1107 3 0.07770222176177555 1107 4 0.16305411047610363 1107 5 0.002314476258092668 1107 6 -0.0545875002178704 1107 7 0.11810061736296262 1107 8 0.018747978718963054 1107 9 -0.02867507821783022 1107 10-11 0.04169795205445581 1107 12-13 0.12359906139649723 1107 14-15 0.18192899436001397 1107 16-17 0.13707927653934604 1107 18-19 -0.027767859889152646 1107 20-21 0.19253328352458965 1107 22-23 -0.010667832167889912 1107 24-25 -0.012719948694730476 1107 26-27 0.19012974117172377 1107 28-29 0.3165659151130029 1107 30-31 0.012356847967488704 1107 32-33 -0.13415305641617437 1107 34-35 0.005423185625268445 1107 36-37 0.1768222878331258 1107 38-39 0.1414456855064472 1107 40-41 0.16985810799464218 1107 42-43 0.33697423956496664 1107 44-45 0.4153124096371883 1107 46-47 0.4255833049933173 1107 48-49 0.5954379146476185 1107 50-51 0.5848237536149199 1107 52-53 0.7632729178537474 1107 54-55 0.5032059802740818 1107 56-57 0.5653654071877483 1107 58-59 0.7746745563788053 1107 60-61 0.8187011804528908 1107 62-63 0.5927793804159833 1107 64-65 0.38956613688674224 1107 66-67 0.37755151130242837 1107 68-69 0.7539438577659396 1107 70-71 0.7504996985299783 1107 72-73 0.7089201427932785 1107 74-75 0.2132106112686678 1107 76-77 0.14863436757281434 1107 78-79 0.5325693194616683 1107 80-81 0.7905033906963901 1107 82-83 0.9140714647560806 1107 84-85 0.21577545851070212 1107 86-87 0.5341575818837185 1107 88-89 0.3310230320664438 1107 90-91 0.19736168180339675 1107 92-93 0.4769155037205053 1107 94-95 0.06416244906943547 1107 96-97 0.09165908689565683 1107 98-99 -0.40828024296596155 1107 100-101 -1.2913419390146892 1108 1 0.08683783039865745 1108 2 0.015497422074233214 1108 3 0.1025562667993043 1108 4 0.1084586142292352 1108 5 0.06388244956918498 1108 6 0.012751949323408951 1108 7 0.171428390507252 1108 8 0.12391895453214374 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84-85 0.057688444726785804 2315 86-87 -0.08452029949377504 2315 88-89 0.24521403136302666 2315 90-91 0.01886219813905754 2315 92-93 0.20759631848321192 2315 94-95 0.1505581268161187 2315 96-97 0.08163319528124902 2315 98-99 0.6208096593044168 2315 100-101 1.175208489768405 2316 1 -0.40657662915753434 2316 2 -0.46612033025218125 2316 3 -0.40725046190506475 2316 4 -0.23615126408142828 2316 5 -0.40631082172644994 2316 6 -0.4299252160309166 2316 7 -0.3260805572379226 2316 8 -0.32110609915432775 2316 9 -0.4553311498785959 2316 10-11 -0.3239814526988525 2316 12-13 -0.42420849476907563 2316 14-15 -0.4878824626940954 2316 16-17 -0.46298573826958034 2316 18-19 -0.7357285620490899 2316 20-21 -0.6425891610322267 2316 22-23 -0.6014357293000145 2316 24-25 -0.6038490392097913 2316 26-27 -0.557266054537294 2316 28-29 -0.5001356669140122 2316 30-31 -0.46283639049238445 2316 32-33 -0.7189251433915373 2316 34-35 -0.6666421181383626 2316 36-37 -0.6988982929658363 2316 38-39 -0.560634788379268 2316 40-41 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#Quality Count 3 2.0 4 4.0 5 12.0 6 33.0 7 63.0 8 148.0 9 305.0 10 535.0 11 949.0 12 1331.0 13 2087.0 14 2617.0 15 3543.0 16 4992.0 17 7019.0 18 6975.0 19 4835.0 20 4219.0 21 4161.0 22 4708.0 23 4876.0 24 5509.0 25 6003.0 26 6296.0 27 6462.0 28 7649.0 29 9211.0 30 11405.0 31 13851.0 32 17600.0 33 22437.0 34 31578.0 35 48049.0 36 68969.0 37 109801.0 38 86497.0 39 5747.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.117351926478975 21.401969677081883 15.654955832136652 21.825722564302495 2 15.250438377366994 22.479991379394797 40.44298155386409 21.826588689374123 3 18.10680239323875 26.406537130856243 32.061484450609086 23.425176025295922 4 12.08418792619452 20.374081496325985 37.22870961865079 30.3130209588287 5 12.177725364514872 38.570698156415716 35.49492333580816 13.75665314326125 6 28.55542082054276 38.456354671310784 18.063067739932258 14.925156768214205 7 24.909006852401085 35.149996669787924 21.598188364630797 18.342808113180194 8 23.783199275972716 36.114386907956856 21.2165852397165 18.885828576353926 9 24.44003463420559 17.48831487351071 21.742758747683546 36.32889174460016 10-11 22.864158690482252 28.24372450918551 28.51307989766454 20.379036902667696 12-13 24.305229742132077 26.565185920158598 28.346861596416666 20.782722741292663 14-15 21.46801625143493 28.378402203425026 27.528708387041167 22.62487315809888 16-17 19.586178794895602 31.248947032296233 28.645521116165355 20.519353056642807 18-19 19.086131037968336 29.3615003976665 31.829873177688363 19.7224953866768 20-21 20.545449559040744 28.31023080328633 31.853870294116494 19.290449343556432 22-23 19.959528128538352 29.11908054803537 31.339156633586562 19.58223468983972 24-25 19.775339449397503 29.134465255779602 31.967699658542603 19.12249563628029 26-27 19.929935999984323 29.15738047632156 31.83258700935852 19.080096514335594 28-29 19.573140927750934 29.397325298584875 31.873476220468433 19.15605755319576 30-31 19.83154475749419 29.424619266468415 32.03029804373293 18.71353793230446 32-33 19.58350800622162 29.404107522753186 31.826836807854598 19.185547663170595 34-35 19.882803775669736 29.24449381497024 31.82527927652588 19.047423132834144 36-37 19.410684971222825 29.42902087079851 31.573008615522046 19.58728554245662 38-39 19.364339915938814 28.78130867851934 32.217806827743125 19.636544577798716 40-41 19.567708721050963 28.72982725587992 32.105762185924235 19.596701837144884 42-43 19.54650884884673 28.793426347113005 31.86956129937194 19.79050350466832 44-45 19.610639439897508 29.104290488522523 31.499594497706074 19.7854755738739 46-47 19.87476406847328 28.620318467157645 30.894566155347015 20.61035130902206 48-49 19.7609863792992 28.54391907113599 31.13083356024707 20.564260989317752 50-51 19.806800194336045 28.853123481749655 30.824693999091007 20.515382324823296 52-53 19.762459838570646 29.11703628242301 30.87620484288065 20.244299036125696 54-55 19.891974915611524 28.917476593585494 30.413743517809248 20.77680497299373 56-57 20.12535241898037 29.213794643819273 29.836932137672147 20.823920799528214 58-59 19.628620189001246 29.534824939700254 30.234027670284323 20.602527201014173 60-61 19.858070582704006 29.82848971036212 29.633961192246282 20.679478514687588 62-63 19.548580497266794 30.015576323987535 29.583455788710594 20.85238739003507 64-65 19.67679174267168 29.767938979158785 29.541834269558354 21.013435008611182 66-67 19.673166951892927 29.7900623709992 29.353847419788075 21.1829232573198 68-69 19.78119632662219 29.62589196397166 29.334996340535017 21.25791536887113 70-71 19.775116012746953 29.22933444880598 29.44269798088349 21.552851557563578 72-73 19.690078900583305 29.126706206747933 29.43398259387504 21.749232298793718 74-75 19.958738082829477 28.976504610127783 29.17448736084389 21.89026994619885 76-77 19.677646261249173 28.850189372045044 29.622176808635288 21.849987558070502 78-79 19.71210038571455 28.679449359901948 29.54267793863641 22.065772315747086 80-81 19.61296768853254 28.734038957922113 29.47695291645647 22.176040437088883 82-83 19.73742870608339 28.682469804497163 29.445453111359114 22.134648378060334 84-85 19.950702721343017 28.462320694864346 29.351376346319256 22.235600237473378 86-87 19.814724094685687 28.519262423577363 29.328558551497103 22.33745493023985 88-89 20.019221179741542 28.170490492765605 29.177202230910833 22.63308609658202 90-91 19.94378252124321 28.01527218645627 29.336451519037816 22.7044937732627 92-93 19.83051262582968 28.01175634965343 29.336990864336627 22.820740160180264 94-95 19.83809237394314 28.123809178618554 29.214981157471037 22.82311728996727 96-97 19.888462544437186 28.054618675323734 29.078302506898844 22.978616273340236 98-99 20.07693528384947 27.892713776058965 28.870224850077093 23.160126090014476 100-101 20.26446174130591 27.705399141513187 28.764695856263213 23.265443260917692 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3498.0 1 2014.5 2 596.5 3 700.5 4 516.0 5 410.0 6 717.0 7 1323.0 8 1920.5 9 2072.0 10 1561.5 11 941.5 12 795.5 13 801.0 14 807.5 15 832.5 16 879.0 17 960.5 18 1057.0 19 1133.0 20 1282.5 21 1460.0 22 1637.5 23 1900.0 24 2267.0 25 2756.5 26 3528.0 27 4513.5 28 5464.0 29 6396.0 30 7608.5 31 8947.5 32 10250.0 33 11793.5 34 13254.5 35 14505.0 36 15698.5 37 16693.0 38 17364.0 39 17765.0 40 18400.5 41 19020.5 42 19452.0 43 20102.0 44 20594.5 45 20944.5 46 21041.0 47 20865.0 48 21523.0 49 21117.0 50 19438.5 51 18004.0 52 15815.5 53 13291.0 54 11272.5 55 9544.5 56 7880.0 57 6456.5 58 5045.0 59 3901.5 60 3019.5 61 2214.5 62 1525.5 63 955.0 64 610.5 65 424.5 66 305.5 67 193.0 68 134.5 69 112.5 70 90.0 71 68.5 72 55.0 73 40.0 74 30.5 75 30.0 76 27.0 77 17.0 78 11.5 79 11.5 80 8.0 81 5.0 82 3.5 83 2.5 84 1.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.00763989829140531 2 0.014300322442886863 3 0.007835793119390061 4 1.9589482798475157E-4 5 9.794741399237576E-4 6 0.002154843107832267 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 6.856318979466304E-4 14-15 0.0 16-17 0.003134317247756025 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0037220017317102796 26-27 0.01714079744866576 28-29 0.005583002597565419 30-31 0.003134317247756025 32-33 0.0 34-35 0.01018653105520708 36-37 7.835793119390063E-4 38-39 0.0032322646617484003 40-41 0.002350737935817019 42-43 0.003134317247756025 44-45 0.0 46-47 2.9384224197712735E-4 48-49 9.794741399237576E-4 50-51 0.004309686215664534 52-53 0.007444003463420559 54-55 0.008031687947374813 56-57 0.015083901754825869 58-59 0.02174432590630742 60-61 0.00254663276380177 62-63 0.017238744862658136 64-65 0.018610008658551395 66-67 0.012635216405016475 68-69 0.030853435407598367 70-71 0.01596542848075725 72-73 0.004995318113611164 74-75 0.015671586238780122 76-77 0.020960746594368418 78-79 0.024780695740071072 80-81 0.01087216295315371 82-83 0.03496722679527815 84-85 0.020568956938398913 86-87 0.030951382821590746 88-89 0.020275114696421785 90-91 0.027131433675888092 92-93 0.022625852632238805 94-95 0.012439321577031724 96-97 0.013027006060985977 98-99 0.009598846571252826 100-101 0.00881526725931382 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 510478.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.24262169987281 #Duplication Level Percentage of deduplicated Percentage of total 1 79.79788446322141 54.456168418737775 2 13.09282224608846 17.869770310469875 3 3.875117118086225 7.933444545967788 4 1.4684505884135854 4.008436719602558 5 0.7015884359485963 2.393911711172275 6 0.3502479809449542 1.4341104278862462 7 0.2226017157138148 1.063364727264035 8 0.12444328972354482 0.6793869074953232 9 0.08316574325420577 0.5107903519756962 >10 0.25760729126478626 2.844050544467186 >50 0.013178812618849905 0.6698252180844505 >100 0.009454364129116828 1.2800190373771623 >500 0.0011459835308019048 0.5718734816447172 >1k 0.0022919670616038095 4.284847597854913 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4471 0.8758457759198242 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4372 0.8564521879493338 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3753 0.7351932894267725 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2672 0.5234309803752561 No Hit CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT 2645 0.5181418200196679 No Hit ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT 1661 0.32538130928267234 TruSeq Adapter, Index 3 (95% over 21bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1317 0.2579934884559178 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1025 0.20079219868437032 No Hit ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT 882 0.17277923828255087 RNA PCR Primer, Index 3 (95% over 23bp) ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 844 0.16533523481913032 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 618 0.12106300369457644 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 581 0.11381489505914065 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.9589482798475154E-4 0.0 10-11 0.0 0.0 0.0 1.9589482798475154E-4 0.0 12-13 0.0 0.0 0.0 1.9589482798475154E-4 0.0 14-15 0.0 0.0 0.0 5.876844839542546E-4 0.0 16-17 0.0 0.0 0.0 5.876844839542546E-4 0.0 18-19 0.0 0.0 0.0 7.835793119390062E-4 0.0 20-21 0.0 0.0 0.0 0.0011753689679085092 0.0 22-23 0.0 0.0 0.0 0.0028404750057788976 0.0 24-25 0.0 0.0 0.0 0.008325530189351942 0.0 26-27 0.0 0.0 0.0 0.01831616641657427 0.0 28-29 0.0 0.0 0.0 0.04603528457641661 0.0 30-31 0.0 0.0 0.0 0.11332515798917876 0.0 32-33 0.0 0.0 0.0 0.21763915389105898 0.0 34-35 0.0 0.0 0.0 0.38444359992007493 0.0 36-37 0.0 0.0 0.0 0.6195173935017768 0.0 38-39 0.0 0.0 0.0 0.8866199914589855 0.0 40-41 0.0 0.0 0.0 1.1326638954078334 0.0 42-43 0.0 0.0 0.0 1.3677376889895352 0.0 44-45 0.0 0.0 0.0 1.6651060378703881 0.0 46-47 0.0 0.0 0.0 1.9928380850888774 0.0 48-49 0.0 0.0 0.0 2.2999032279549754 0.0 50-51 0.0 0.0 0.0 2.5925701009641946 0.0 52-53 0.0 0.0 0.0 2.8509553790760815 0.0 54-55 0.0 0.0 0.0 3.166541946959516 0.0 56-57 0.0 0.0 0.0 3.518075215778153 0.0 58-59 0.0 0.0 0.0 3.8782278570281186 0.0 60-61 0.0 0.0 0.0 4.234658496546374 0.0 62-63 0.0 0.0 0.0 4.576592918793758 0.0 64-65 0.0 0.0 0.0 4.944189563507145 0.0 66-67 0.0 0.0 0.0 5.365167548846375 0.0 68-69 0.0 0.0 0.0 5.8138646523454485 0.0 70-71 0.0 0.0 0.0 6.274119550695623 0.0 72-73 0.0 0.0 0.0 6.7144911240053435 0.0 74-75 0.0 0.0 0.0 7.177390602533312 0.0 76-77 0.0 0.0 0.0 7.679664941486215 0.0 78-79 0.0 0.0 0.0 8.223566931385877 0.0 80-81 0.0 0.0 0.0 8.783140507524319 0.0 82-83 0.0 0.0 0.0 9.342420241420786 0.0 84-85 0.0 0.0 0.0 9.917665403798008 0.0 86-87 0.0 0.0 0.0 10.510345205865875 0.0 88-89 0.0 0.0 0.0 11.150235661478067 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2790 0.0 33.53752 1 TACGAAA 65 0.0058426885 29.229113 2 GTATCAA 3840 0.0 25.727707 1 CCTATAC 115 1.2808823E-4 24.781204 3 CTATACT 135 1.3414159E-5 24.628235 4 GTACATG 2180 0.0 23.312897 1 TATCAAC 4415 0.0 22.376986 2 TACATGG 2280 0.0 22.290403 2 ATCAACG 4400 0.0 22.129427 3 GTGTAGC 345 0.0 22.027735 1 TATACCA 130 2.9395358E-4 21.921833 5 TCAACGC 4445 0.0 21.905394 4 CAACGCA 4515 0.0 21.565777 5 AACGCAG 4620 0.0 21.075644 6 ACATGGG 2275 0.0 20.877937 3 GAGTACT 3145 0.0 20.539375 12-13 CATGGGG 880 0.0 19.970457 4 GTGTTAT 215 1.0769727E-6 19.882593 1 GTACTTT 3290 0.0 19.634146 14-15 GTATTAG 170 7.946271E-5 19.557714 1 >>END_MODULE