FastQCFastQC Report
Thu 26 May 2016
SRR938095_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938095_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2310980
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76360.33042259128162077No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62070.2685873525517313No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37320.16148993067875966No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA25220.10913119109641797No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT23900.10341932859652615No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68200.030.2170351
GTATCAA93750.028.7494561
GTACATG62000.024.0057541
ATCAACG110900.023.9563943
TCAACGC112250.023.7106134
CAACGCA112150.023.6046245
TATCAAC112450.023.5416492
GGACCGA8350.023.3321046
TACATGG60500.023.3314762
AACGCAG115800.023.0613256
GAGTACT70750.022.32251412-13
ACCGAGT9000.022.1677828
TAAGGTG11650.021.6217615
ACATGGG62500.021.5202623
GTACTTT74500.021.07412114-15
ACGCAGA127550.020.8183777
ACCGATT1150.003723314320.6532148
CGCAGAG128300.020.4745318
AGAGTAC100250.020.32638710-11
AGTACTT74050.019.59584612-13
Produced by FastQC (version 0.11.5)