FastQCFastQC Report
Thu 26 May 2016
SRR938095_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938095_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2310980
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76370.330465862967226No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63880.2764195276462799No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40620.1757695869284892No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT25600.11077551514941714No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA23640.10229426477078989No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68350.030.756661
GTATCAA94550.028.1696831
ATCAACG111800.023.6069973
TCAACGC112250.023.5537034
CAACGCA112600.023.480495
TATCAAC112700.023.4642312
AACGCAG116950.022.6609556
GTACATG62650.022.0916081
GAGTACT75950.021.91818212-13
TACATGG63150.021.3141752
GTACTTT77850.020.98669614-15
GGACCGA8650.020.8646816
TAAGGTG13000.020.849825
ACATGGG64200.020.4438953
ACGCAGA130350.020.2585357
CGCAGAG130350.020.1856638
AGAGTAC105800.019.05619810-11
TAGACCG1250.00599478419.0208855
AGTACTT78800.018.65428712-13
AGGACCG11600.018.4469815
Produced by FastQC (version 0.11.5)