##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938095_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2310980 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.46990064820985 33.0 31.0 34.0 31.0 34.0 2 32.698035032756664 34.0 31.0 34.0 31.0 34.0 3 32.76837705215969 34.0 31.0 34.0 31.0 34.0 4 36.229893811283524 37.0 35.0 37.0 35.0 37.0 5 36.15414369661356 37.0 35.0 37.0 35.0 37.0 6 36.20141628226986 37.0 37.0 37.0 35.0 37.0 7 36.16954322408675 37.0 37.0 37.0 35.0 37.0 8 36.163170602947666 37.0 37.0 37.0 35.0 37.0 9 37.851343153121185 39.0 38.0 39.0 35.0 39.0 10-11 37.82340176029217 39.0 38.0 39.0 35.0 39.0 12-13 37.74851881020173 39.0 38.0 39.0 35.0 39.0 14-15 39.00051969294412 40.0 38.0 41.0 36.0 41.0 16-17 38.91176730218349 40.0 38.0 41.0 35.0 41.0 18-19 39.15104977109279 40.0 38.5 41.0 36.0 41.0 20-21 39.1130249937256 40.0 39.0 41.0 36.0 41.0 22-23 39.01219590822941 40.0 38.5 41.0 35.0 41.0 24-25 38.914917913612406 40.0 38.0 41.0 35.0 41.0 26-27 38.76046482444677 40.0 38.0 41.0 35.0 41.0 28-29 38.64032228751439 40.0 38.0 41.0 34.5 41.0 30-31 38.413365109174464 40.0 38.0 41.0 34.0 41.0 32-33 38.20160970670452 40.0 38.0 41.0 34.0 41.0 34-35 38.117926810270966 40.0 38.0 41.0 34.0 41.0 36-37 38.0696416671715 40.0 38.0 41.0 33.5 41.0 38-39 37.89275653618811 40.0 37.5 41.0 33.0 41.0 40-41 37.68464590779669 40.0 37.0 41.0 33.0 41.0 42-43 37.44354819167626 40.0 37.0 41.0 32.5 41.0 44-45 37.1917837886957 40.0 37.0 41.0 31.5 41.0 46-47 36.85852127668781 39.5 36.0 41.0 31.0 41.0 48-49 36.47302724385325 39.0 36.0 41.0 30.0 41.0 50-51 36.10704722671767 39.0 35.0 40.0 29.5 41.0 52-53 35.58469523751828 38.5 35.0 40.0 28.0 41.0 54-55 35.218251780629856 38.0 34.0 40.0 27.0 41.0 56-57 34.84250512769474 38.0 34.0 40.0 26.0 41.0 58-59 34.34255748643433 37.5 33.5 40.0 25.5 41.0 60-61 33.859486884352094 37.0 33.0 40.0 23.5 41.0 62-63 33.30244311936927 36.5 32.5 39.0 22.5 40.5 64-65 32.73565392171287 36.0 31.5 39.0 20.5 40.0 66-67 32.15875451107323 35.0 31.0 38.5 18.5 40.0 68-69 31.53696094297657 35.0 30.5 38.0 15.0 40.0 70-71 30.911164094886153 35.0 30.0 37.5 9.5 39.5 72-73 30.22835831551982 34.0 29.0 37.0 7.0 39.0 74-75 29.56303451349644 34.0 28.5 36.0 4.0 39.0 76-77 28.26758820933111 32.5 27.0 35.0 2.0 37.0 78-79 28.914706531428223 34.0 28.0 35.0 2.0 37.0 80-81 28.634603068827943 34.0 28.0 35.0 2.0 37.0 82-83 28.072208327203178 33.5 26.5 35.0 2.0 36.5 84-85 27.4132318323828 33.0 26.0 35.0 2.0 36.0 86-87 26.696332508286527 32.5 25.0 35.0 2.0 35.5 88-89 25.897718284018033 32.0 23.5 34.5 2.0 35.0 90-91 25.238120407792366 32.0 20.0 34.0 2.0 35.0 92-93 24.293505785424365 31.0 14.0 34.0 2.0 35.0 94-95 23.39077058217726 31.0 4.5 34.0 2.0 35.0 96-97 22.341200919090603 30.0 2.0 34.0 2.0 35.0 98-99 20.929656898804836 29.5 2.0 34.0 2.0 35.0 100-101 17.040508139404064 22.0 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.16799436476140528 1101 2 0.20501875641573974 1101 3 0.17085219170142096 1101 4 0.04539981660213499 1101 5 -0.07807560227797694 1101 6 -0.018455853550591428 1101 7 0.049599626678165976 1101 8 0.05271611226908135 1101 9 0.04662317442970476 1101 10-11 0.132343776449666 1101 12-13 0.2116402613115156 1101 14-15 0.34257445718493784 1101 16-17 0.3674402583567016 1101 18-19 0.32913058893225156 1101 20-21 0.2512229772823815 1101 22-23 0.3135142024102606 1101 24-25 0.3202349130329196 1101 26-27 0.4640809934935888 1101 28-29 0.38792726799989197 1101 30-31 0.5077435053123196 1101 32-33 0.48790762943112753 1101 34-35 0.45648958388604655 1101 36-37 0.4411974335976723 1101 38-39 0.5565437945152354 1101 40-41 0.7624204934019261 1101 42-43 0.7898653599776182 1101 44-45 0.6781649229280688 1101 46-47 0.8136855436551755 1101 48-49 0.9744284064492845 1101 50-51 0.8625005528301344 1101 52-53 1.0490219390219906 1101 54-55 1.1499781854650948 1101 56-57 1.2015192232484821 1101 58-59 1.1456215029989139 1101 60-61 1.2299633507009275 1101 62-63 1.331492664496821 1101 64-65 1.4641050355384593 1101 66-67 1.5448840331893408 1101 68-69 1.7088147807217275 1101 70-71 1.8639628303288127 1101 72-73 1.9259442826473823 1101 74-75 2.1499215288153053 1101 76-77 1.9850193631139277 1101 78-79 2.1854845862209444 1101 80-81 2.272493027984222 1101 82-83 2.3389852938224713 1101 84-85 2.435915855756136 1101 86-87 2.6478429417113887 1101 88-89 2.932945672776121 1101 90-91 3.1918828838656097 1101 92-93 3.5626672283934973 1101 94-95 3.7294836378409215 1101 96-97 3.8953949009948623 1101 98-99 4.218431002743966 1101 100-101 3.8790865160700463 1102 1 0.19839640859002827 1102 2 0.19817288880035022 1102 3 0.23846121085812655 1102 4 0.15631482717807188 1102 5 -8.986963877788412E-4 1102 6 0.07073277867608141 1102 7 0.06643181325753744 1102 8 0.15041201368003243 1102 9 0.17984929855637688 1102 10-11 0.21193544799687203 1102 12-13 0.34317149068354524 1102 14-15 0.41344871306635866 1102 16-17 0.2749939486312556 1102 18-19 0.32341965187199406 1102 20-21 0.30253930600125756 1102 22-23 0.4008623375608522 1102 24-25 0.4120492714686179 1102 26-27 0.3131473244220828 1102 28-29 0.30131586664034415 1102 30-31 0.43605994707069584 1102 32-33 0.38017086633354324 1102 34-35 0.45128790606352 1102 36-37 0.46103144695121756 1102 38-39 0.5497145571969355 1102 40-41 0.6906228489962913 1102 42-43 0.6761957674640868 1102 44-45 0.5415549403770825 1102 46-47 0.5893641179246956 1102 48-49 0.7007508174376511 1102 50-51 0.704646005362612 1102 52-53 0.8873831267461796 1102 54-55 0.9966987141364285 1102 56-57 0.9670942524038253 1102 58-59 1.119580551066747 1102 60-61 1.1495806352315867 1102 62-63 1.3923545511586681 1102 64-65 1.495841806078971 1102 66-67 1.4880186511414095 1102 68-69 1.6223891037290805 1102 70-71 1.6492061513456484 1102 72-73 1.7606250576983093 1102 74-75 1.887466599246359 1102 76-77 1.8801772020777605 1102 78-79 1.9980005478172842 1102 80-81 2.2220161222335797 1102 82-83 2.180468674457174 1102 84-85 2.507511959568266 1102 86-87 2.6664972506329967 1102 88-89 2.7283073084716705 1102 90-91 2.9540790553153684 1102 92-93 3.067273704394001 1102 94-95 3.1137511907124775 1102 96-97 3.3941964729417116 1102 98-99 3.8320860582845064 1102 100-101 4.101022366385276 1103 1 0.15237612475329598 1103 2 0.15481470082790594 1103 3 0.14447543874218383 1103 4 0.08577919979219217 1103 5 -0.021269947915286025 1103 6 0.03332195077688738 1103 7 0.1277559941719133 1103 8 0.12443945651796184 1103 9 0.07250438321354835 1103 10-11 0.14541233985126212 1103 12-13 0.1994194404912406 1103 14-15 0.34913080078395353 1103 16-17 0.35860079113684407 1103 18-19 0.2450892383729837 1103 20-21 0.21140645534176628 1103 22-23 0.2288872498757044 1103 24-25 0.34390042840791324 1103 26-27 0.1953860803563785 1103 28-29 0.24651242375915672 1103 30-31 0.3469437196408336 1103 32-33 0.36198385815440304 1103 34-35 0.3827427675876649 1103 36-37 0.3915152833251483 1103 38-39 0.473584608348105 1103 40-41 0.576449892069256 1103 42-43 0.7590048373346434 1103 44-45 0.8183198630735689 1103 46-47 0.831612363320545 1103 48-49 0.8640436786229557 1103 50-51 0.9022187579012453 1103 52-53 0.8751858342616927 1103 54-55 1.0895075406874568 1103 56-57 1.0171896877294984 1103 58-59 1.1777647836009137 1103 60-61 1.2340798746327692 1103 62-63 1.3888978368013838 1103 64-65 1.3584081782388893 1103 66-67 1.461491415575665 1103 68-69 1.8212678963595614 1103 70-71 1.7897216012344437 1103 72-73 1.8929784892245856 1103 74-75 1.9591417730846423 1103 76-77 1.9007104413275435 1103 78-79 2.0913234995174577 1103 80-81 2.1763008889892816 1103 82-83 2.5033041219668455 1103 84-85 2.5721538472952297 1103 86-87 2.627434328203261 1103 88-89 2.9151043853930823 1103 90-91 3.3339981650903248 1103 92-93 3.4020784449793346 1103 94-95 3.610051382123835 1103 96-97 3.749543239315315 1103 98-99 4.37733335203065 1103 100-101 4.024854342536358 1104 1 0.1578520651165647 1104 2 0.1608948355357782 1104 3 0.13060085369668428 1104 4 0.06069655218510661 1104 5 0.010437381717359528 1104 6 0.016870528345485525 1104 7 0.09433091598710774 1104 8 0.12789512204146547 1104 9 0.17430211885793057 1104 10-11 0.18248833102178708 1104 12-13 0.2545099065791163 1104 14-15 0.296071122860738 1104 16-17 0.27592443333019645 1104 18-19 0.2370892418059043 1104 20-21 0.2772333862748013 1104 22-23 0.2683499803535341 1104 24-25 0.2646690082422438 1104 26-27 0.2977789989240236 1104 28-29 0.3453022756972146 1104 30-31 0.2867534327515102 1104 32-33 0.30937110381776023 1104 34-35 0.3397808163832323 1104 36-37 0.3939575930640018 1104 38-39 0.5163869286603671 1104 40-41 0.5494613288502705 1104 42-43 0.6199798473759444 1104 44-45 0.7201530865533883 1104 46-47 0.7964581650280778 1104 48-49 0.7841173062263991 1104 50-51 0.9533469878521217 1104 52-53 1.1040654227916136 1104 54-55 1.2225586019754147 1104 56-57 1.1348492413809268 1104 58-59 1.2150898762039972 1104 60-61 1.243483721908916 1104 62-63 1.2548668907222904 1104 64-65 1.6152272494960584 1104 66-67 1.6815940942850247 1104 68-69 1.6725860956544416 1104 70-71 1.6596968756107593 1104 72-73 1.918266897618775 1104 74-75 2.0206267008387933 1104 76-77 2.065149355906133 1104 78-79 2.1737024804201113 1104 80-81 2.3429083377443334 1104 82-83 2.460692114290829 1104 84-85 2.6201641254649317 1104 86-87 2.726608238336219 1104 88-89 2.9202685734897678 1104 90-91 3.2179207595557635 1104 92-93 3.309671254036761 1104 94-95 3.4255270821825405 1104 96-97 3.8524217018466835 1104 98-99 4.06356789100634 1104 100-101 3.7728967185306175 1105 1 0.14149636866653736 1105 2 0.1386196440575418 1105 3 0.10983062878879224 1105 4 0.12823516400249702 1105 5 -0.013202256576768434 1105 6 0.004490135749350088 1105 7 0.06883059540132308 1105 8 0.1104172749807617 1105 9 0.10534666643049206 1105 10-11 0.047284137751717026 1105 12-13 0.18498846065492813 1105 14-15 0.24198627382111226 1105 16-17 0.24571278649086992 1105 18-19 0.1997633809116337 1105 20-21 0.23370026596941074 1105 22-23 0.1919157654795569 1105 24-25 0.24224837881813244 1105 26-27 0.21314435229306383 1105 28-29 0.2250374401233941 1105 30-31 0.29503094963094156 1105 32-33 0.433535864434333 1105 34-35 0.34009086318433646 1105 36-37 0.34493165592772357 1105 38-39 0.3644034722518015 1105 40-41 0.36502242623728876 1105 42-43 0.4742982000989926 1105 44-45 0.6340287297825711 1105 46-47 0.657534112743491 1105 48-49 0.7510753907895875 1105 50-51 0.7639385037511275 1105 52-53 0.829598825742444 1105 54-55 0.842675189208272 1105 56-57 0.8898236406890874 1105 58-59 1.0447939857644357 1105 60-61 1.128510559174586 1105 62-63 1.2380383452984773 1105 64-65 1.2765150207042097 1105 66-67 1.3595975936284006 1105 68-69 1.603845337337578 1105 70-71 1.8134894234868462 1105 72-73 1.7943369053634193 1105 74-75 1.6336122116461667 1105 76-77 1.4991834612549972 1105 78-79 1.6233026615701505 1105 80-81 1.8471668892747353 1105 82-83 1.9698767865566076 1105 84-85 2.016010983824632 1105 86-87 2.060916477651798 1105 88-89 2.168817405911131 1105 90-91 2.489592519059819 1105 92-93 2.545144622933634 1105 94-95 2.7796470009380343 1105 96-97 3.114968008124343 1105 98-99 3.2134421459003413 1105 100-101 3.0721851264939914 1106 1 0.1136758096971846 1106 2 0.08416931490481971 1106 3 0.04108329878920358 1106 4 0.05668813750624224 1106 5 -0.059011010449047774 1106 6 0.006399687197010451 1106 7 0.05687807469873718 1106 8 0.10532565302905539 1106 9 0.1831761036644437 1106 10-11 0.16547692883272447 1106 12-13 0.07030002398110469 1106 14-15 -0.021965691965789347 1106 16-17 0.028103912786164642 1106 18-19 -0.10319459794322228 1106 20-21 0.028010486639210797 1106 22-23 -0.03068193938760544 1106 24-25 -0.04593835108419597 1106 26-27 -0.10124040715620453 1106 28-29 0.06347785266996908 1106 30-31 0.07850418084201749 1106 32-33 0.16223764188416112 1106 34-35 0.14316805616882533 1106 36-37 0.2613261298794356 1106 38-39 0.11776769692232847 1106 40-41 0.26449322756684523 1106 42-43 0.3615375737630302 1106 44-45 0.42308686280428276 1106 46-47 0.4658345076185597 1106 48-49 0.35182379275025966 1106 50-51 0.5810808386593678 1106 52-53 0.5278574299787735 1106 54-55 0.757907470307849 1106 56-57 0.6885187966344972 1106 58-59 0.8436858353629688 1106 60-61 0.8617338754489055 1106 62-63 0.7149783330601949 1106 64-65 0.9523618516081385 1106 66-67 1.1687667832099677 1106 68-69 0.9110484598589856 1106 70-71 0.8600738420648533 1106 72-73 1.0453824441948605 1106 74-75 1.3299437784077206 1106 76-77 1.4451145400308079 1106 78-79 1.532409906176099 1106 80-81 1.4025758584666725 1106 82-83 1.6877961580025236 1106 84-85 1.9317638848144973 1106 86-87 2.0718836945390144 1106 88-89 2.217132941173876 1106 90-91 2.323581240282504 1106 92-93 2.410082163037984 1106 94-95 2.4472496047910255 1106 96-97 3.1211172931970133 1106 98-99 3.3376875175614984 1106 100-101 3.021132979683241 1107 1 0.1340839449180109 1107 2 0.11392560025435472 1107 3 0.09996728054873927 1107 4 0.05838937165679425 1107 5 -0.045760779635983795 1107 6 0.031410538306829494 1107 7 0.050932589182494326 1107 8 0.1108751785543376 1107 9 0.09499441986666568 1107 10-11 0.17094763535137503 1107 12-13 0.08429996783605986 1107 14-15 0.24188480987472616 1107 16-17 0.26108062692288314 1107 18-19 0.17230992713152915 1107 20-21 0.1733210305806594 1107 22-23 0.23756986289781423 1107 24-25 0.2516082085692233 1107 26-27 0.2683410315272212 1107 28-29 0.2642229916574266 1107 30-31 0.3453985883046755 1107 32-33 0.38927711558128664 1107 34-35 0.31874951566125986 1107 36-37 0.4542659296300826 1107 38-39 0.34653412155672925 1107 40-41 0.40372989836978945 1107 42-43 0.5882106323272254 1107 44-45 0.72900570887046 1107 46-47 0.6619570051150916 1107 48-49 0.8916038337361414 1107 50-51 0.9088155686301604 1107 52-53 0.9173347294177745 1107 54-55 0.9906485126700701 1107 56-57 1.0036729138037117 1107 58-59 1.1786714231652766 1107 60-61 1.069538076079887 1107 62-63 1.2708606161376181 1107 64-65 1.3737338559214862 1107 66-67 1.4256833718919566 1107 68-69 1.634371072629552 1107 70-71 1.6075439286322641 1107 72-73 1.7253095498647433 1107 74-75 1.7134690159002837 1107 76-77 1.5723734701849956 1107 78-79 1.6511748368811325 1107 80-81 1.7502609255923929 1107 82-83 2.0532333659525825 1107 84-85 2.077549305909212 1107 86-87 2.4003984676551156 1107 88-89 2.4854375512121756 1107 90-91 2.750314802004464 1107 92-93 3.1054829218598705 1107 94-95 3.158941162755191 1107 96-97 3.2008006586959645 1107 98-99 3.443579920488972 1107 100-101 3.5571520878447096 1108 1 0.27093836775374314 1108 2 0.19408095891439103 1108 3 0.16719288983042446 1108 4 0.1319880471246293 1108 5 0.06444676982390263 1108 6 0.13069629927483106 1108 7 0.15958530718100405 1108 8 0.17076419919101937 1108 9 0.1856097235660812 1108 10-11 0.1616239372426307 1108 12-13 0.2502035346741991 1108 14-15 0.37883602065771527 1108 16-17 0.3730418743981474 1108 18-19 0.2545946206311811 1108 20-21 0.29951539696194374 1108 22-23 0.33880383142150094 1108 24-25 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48-49 -0.24537406360968816 2316 50-51 0.02437232298581904 2316 52-53 0.17953646173963733 2316 54-55 0.4764998025193776 2316 56-57 0.42010131059295475 2316 58-59 0.4469867018002347 2316 60-61 0.5105383934388215 2316 62-63 0.6304359735090159 2316 64-65 0.9290448050051907 2316 66-67 1.1445936024309091 2316 68-69 1.334571916212706 2316 70-71 1.4572977874511643 2316 72-73 1.471869531120678 2316 74-75 1.8530125237375792 2316 76-77 1.9225361855741987 2316 78-79 1.9706663740598422 2316 80-81 1.9520624347101538 2316 82-83 2.165595390438522 2316 84-85 2.299514220617535 2316 86-87 2.396397639413479 2316 88-89 2.7721336608997156 2316 90-91 3.238702456524752 2316 92-93 3.2595387907356503 2316 94-95 3.7959599206902084 2316 96-97 4.115874806878015 2316 98-99 4.487700081705302 2316 100-101 4.94388486343518 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 9.0 4 15.0 5 29.0 6 93.0 7 203.0 8 535.0 9 1059.0 10 1736.0 11 3012.0 12 4595.0 13 6832.0 14 9018.0 15 12749.0 16 16383.0 17 22071.0 18 22909.0 19 19968.0 20 19881.0 21 21180.0 22 23700.0 23 25864.0 24 29658.0 25 33351.0 26 37332.0 27 43560.0 28 52907.0 29 62896.0 30 75528.0 31 89935.0 32 110264.0 33 137114.0 34 178390.0 35 241429.0 36 337348.0 37 425637.0 38 232810.0 39 10976.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.84398826471886 18.8986057862898 14.818951267427671 23.438454681563666 2 15.744883796716852 19.83330224538647 40.05792312030083 24.363890837595843 3 19.54972006795568 23.364134945506503 29.728618670156187 27.35752631638163 4 13.037531112934325 16.698255598597278 36.62732330103473 33.63688998743367 5 13.737734320508432 37.17182132072938 34.507834872003706 14.582609486758482 6 32.8924662134094 36.047323728156414 16.834921695422196 14.225288363011993 7 28.936857956364832 32.35891266908411 20.248033301889244 18.456196072661815 8 26.13287534541653 35.38008583378985 19.281749977715062 19.20528884307856 9 25.78533782204952 16.993699642575876 20.17196167859523 37.04900085677937 10-11 25.12252377779124 26.34486235276809 27.031973448493712 21.500640420946958 12-13 25.990532155189573 23.5502038096392 27.4694934616483 22.98977057352292 14-15 22.689010722723694 25.16077594786627 25.764524141273398 26.385689188136634 16-17 22.292057914824014 28.381227877350735 26.646249643008595 22.680464564816656 18-19 22.20185808617989 27.101835584903373 28.66736189841539 22.028944430501348 20-21 22.64431107149348 26.129282815082778 29.17195302425811 22.054453089165634 22-23 22.542008297807158 25.999579397759902 28.992454257342796 22.465958047090144 24-25 22.542253920325198 26.228867630850957 28.927692236460118 22.30118621236373 26-27 22.009973858002347 26.516255337342553 28.988472162299356 22.485298642355747 28-29 22.192576309617564 26.985023669612023 28.75405672052549 22.068343300244916 30-31 22.312438878744082 26.397264364036037 28.645920778197993 22.644375979021884 32-33 22.057352292101186 26.35394940674519 28.82335632502229 22.76534197613134 34-35 22.793566822996752 26.499831869785424 28.50813321798634 22.198468089231486 36-37 22.73320885430978 26.11536738981335 28.63334148288702 22.518082272989854 38-39 22.274322737774746 26.547497826520644 28.55514795454929 22.623031481155316 40-41 22.771139188793853 26.36307455819195 28.45490087123907 22.410885381775124 42-43 22.357668516789147 26.29321051497336 28.415795481985324 22.933325486252173 44-45 22.482076185299878 26.472266986081568 28.561654979981753 22.484001848636805 46-47 22.860241728669596 26.839939514824774 27.8458549718853 22.45396378462033 48-49 22.596149685414847 26.640905589836343 28.00498057101316 22.757964153735642 50-51 22.635029294931154 26.931042241819487 28.02436628616431 22.409562177085046 52-53 22.87328319587361 26.850275640637307 27.721356307713613 22.555084855775473 54-55 22.539809950756823 26.827709456594174 27.905693688391935 22.726786904257068 56-57 22.627617992201575 26.899508822884155 27.831581334144534 22.641291850769736 58-59 22.709997490242234 26.70886377207938 27.672199672866054 22.90893906481233 60-61 22.423971648391593 26.972756146742938 27.895611385645918 22.70766081921955 62-63 22.36841080407446 26.97013820976382 28.029645431808152 22.631805554353566 64-65 22.118927900717445 27.05486417017888 27.85162571722819 22.974582211875482 66-67 22.206303042996065 26.983672287805298 27.663622213789047 23.14640245540959 68-69 22.488535596176042 26.754840796509672 27.908626619052924 22.84799698826136 70-71 22.50443534777454 26.84889094669794 27.458026464962913 23.18864724056461 72-73 22.483177043149247 26.777828790309215 27.599493547972525 23.139500618569013 74-75 22.36384564124311 27.40902128101498 27.2118971172403 23.015235960501606 76-77 22.58396870591697 26.855013890211076 27.495910825710308 23.065106578161647 78-79 22.52263109157154 27.01587638144856 27.60149373858709 22.8599987883928 80-81 22.63620537980257 27.108880789380425 27.33690123268211 22.9180125981349 82-83 22.663645378092713 27.04823524855421 27.385523042734444 22.90259633061863 84-85 22.518730430872935 27.269780734902334 27.260541824245628 22.950947009979107 86-87 22.714866159838802 27.298472744172802 27.061466491872572 22.925194604115827 88-89 22.5156859860319 27.427368475711607 26.806116885477156 23.25082865277934 90-91 22.55674252819547 27.197424749699362 27.03177650815411 23.21405621395106 92-93 22.598464720594727 27.033336506590278 27.163346285991224 23.20485248682377 94-95 22.42055318522878 26.964880699962784 27.17464019593419 23.439925918874245 96-97 22.4763043839486 26.74028202802625 27.199417042842505 23.583996545182643 98-99 22.300257899246205 27.00847692321007 26.87656318964249 23.814701987901238 100-101 22.771118746159637 26.41191182961341 26.956356177898556 23.860613246328395 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 551.0 1 384.0 2 273.0 3 344.0 4 297.0 5 310.5 6 584.5 7 1293.5 8 2176.0 9 2551.0 10 2187.0 11 1713.5 12 1693.0 13 1811.5 14 1952.5 15 2157.5 16 2323.5 17 2442.0 18 2703.0 19 3037.0 20 3416.5 21 4080.0 22 4968.0 23 6210.0 24 7995.0 25 10334.0 26 13213.5 27 16645.5 28 19801.0 29 22751.5 30 25977.5 31 29798.5 32 35512.0 33 39105.0 34 40701.5 35 44046.5 36 48897.0 37 53525.0 38 57019.0 39 59970.5 40 63302.5 41 68554.5 42 72086.0 43 74490.0 44 78495.0 45 82235.5 46 84432.0 47 88893.0 48 92784.5 49 91011.0 50 87621.5 51 84209.0 52 82955.5 53 83721.5 54 84138.0 55 79696.0 56 72795.5 57 66943.0 58 60494.5 59 52510.0 60 42192.0 61 34344.0 62 28621.0 63 21870.5 64 15862.5 65 11753.0 66 8561.5 67 6300.0 68 4653.0 69 3277.0 70 2361.5 71 1668.0 72 1131.0 73 851.0 74 625.5 75 421.5 76 289.5 77 158.0 78 71.0 79 42.5 80 31.5 81 19.5 82 10.0 83 4.5 84 6.0 85 4.0 86 2.0 87 1.5 88 0.5 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.014322927935334795 3 0.002682844507524946 4 0.001903954166630607 5 0.1172662679902033 6 1.7308674242096425E-4 7 0.0 8 8.654337121048213E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 3.461734848419285E-4 24-25 4.759885416576517E-4 26-27 0.006014764299128509 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.01172662679902033 36-37 6.49075284078616E-4 38-39 0.00731291486728574 40-41 0.010731378030099784 42-43 0.006144579355944231 44-45 0.003807908333261214 46-47 2.1635842802620532E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 4.3271685605241063E-5 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 4.3271685605241063E-5 68-69 9.736129261179241E-4 70-71 0.0 72-73 4.3271685605241063E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.298150568157232E-4 82-83 0.0019688616950384688 84-85 0.0043920760889319685 86-87 0.003050653835169495 88-89 0.0 90-91 0.002553029450709223 92-93 0.0 94-95 0.0 96-97 1.7308674242096425E-4 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2310980.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.425473365979954 #Duplication Level Percentage of deduplicated Percentage of total 1 72.83069539053038 37.45352986031519 2 14.759755859562434 15.180548636885893 3 5.2185017452192195 8.050917675272727 4 2.475004141765947 5.091130382922993 5 1.2851382161252856 3.304442055247693 6 0.7845752302291694 2.420829156345465 7 0.5068173452089754 1.8244325321222579 8 0.35567806343939035 1.4632730222612558 9 0.24812829031152367 1.1484103306285263 >10 1.3923303614705889 12.940867651385904 >50 0.09189727585814252 3.1392840425336 >100 0.04541744198154533 4.248860059113481 >500 0.0037878989359083053 1.37208914104227 >1k 0.0021043882977265452 1.7542759418832516 >5k 1.6835106381812363E-4 0.6071095120394957 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7637 0.330465862967226 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6388 0.2764195276462799 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4062 0.1757695869284892 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2560 0.11077551514941714 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2364 0.10229426477078989 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.3271685605241063E-5 0.0 5 0.0 0.0 0.0 4.3271685605241063E-5 4.3271685605241063E-5 6 0.0 0.0 0.0 4.3271685605241063E-5 8.654337121048213E-5 7 0.0 0.0 0.0 4.3271685605241063E-5 8.654337121048213E-5 8 0.0 0.0 0.0 4.3271685605241063E-5 8.654337121048213E-5 9 0.0 0.0 0.0 4.3271685605241063E-5 8.654337121048213E-5 10-11 0.0 0.0 0.0 4.3271685605241063E-5 1.298150568157232E-4 12-13 0.0 0.0 0.0 1.298150568157232E-4 3.2453764203930796E-4 14-15 0.0 0.0 0.0 1.298150568157232E-4 3.6780932764454905E-4 16-17 0.0 0.0 0.0 1.514508996183437E-4 4.327168560524107E-4 18-19 0.0 0.0 0.0 2.596301136314464E-4 4.759885416576517E-4 20-21 0.0 0.0 0.0 3.0290179923668747E-4 4.759885416576517E-4 22-23 0.0 0.0 0.0 3.461734848419285E-4 4.759885416576517E-4 24-25 0.0 0.0 0.0 4.543526988550312E-4 4.759885416576517E-4 26-27 0.0 0.0 0.0 0.0010385204545257857 4.976243844602722E-4 28-29 0.0 0.0 0.0 0.004694977888168656 5.192602272628928E-4 30-31 0.0 0.0 0.0 0.015274905018650097 5.192602272628928E-4 32-33 0.0 0.0 0.0 0.03749491557694139 5.408960700655133E-4 34-35 0.0 0.0 0.0 0.0676552804437944 6.49075284078616E-4 36-37 0.0 0.0 0.0 0.1161195683216644 6.49075284078616E-4 38-39 0.0 0.0 0.0 0.1876476646271279 7.139828124864776E-4 40-41 0.0 0.0 0.0 0.26309184847986566 7.356186552890981E-4 42-43 0.0 0.0 0.0 0.3403101714424184 7.356186552890981E-4 44-45 0.0 0.0 0.0 0.4345126310050282 8.005261836969597E-4 46-47 0.0 0.0 0.0 0.5400739080390138 8.870695549074419E-4 48-49 0.0 0.0 0.0 0.6508277873456283 9.087053977100624E-4 50-51 0.0 0.0 0.0 0.7687647664627127 9.952487689205444E-4 52-53 0.0 0.0 0.0 0.8812278773507343 0.0010817921401310266 54-55 0.0 0.0 0.0 1.0038165626703823 0.0010817921401310266 56-57 0.0 0.0 0.0 1.145120252014297 0.0011034279829336472 58-59 0.0 0.0 0.0 1.2962249781477988 0.0011250638257362677 60-61 0.0 0.0 0.0 1.4497312828323914 0.0011250638257362677 62-63 0.0 0.0 0.0 1.6061151546097325 0.0011683355113415088 64-65 0.0 0.0 0.0 1.7668045591047954 0.0011683355113415088 66-67 0.0 0.0 0.0 1.9475720257206899 0.0011683355113415088 68-69 0.0 0.0 0.0 2.145994340063523 0.0011683355113415088 70-71 0.0 0.0 0.0 2.3533955291694433 0.0011683355113415088 72-73 0.0 0.0 0.0 2.5623761347999547 0.0012981505681572319 74-75 0.0 0.0 0.0 2.7807899678924093 0.0014279656249729551 76-77 0.0 0.0 0.0 3.007793230577504 0.0014279656249729551 78-79 0.0 0.0 0.0 3.2609758630537695 0.0014279656249729551 80-81 0.0 2.1635842802620532E-5 0.0 3.524197526590451 0.0014496014677755757 82-83 0.0 4.3271685605241063E-5 0.0 3.7946455616232075 0.0015577806817886784 84-85 0.0 4.3271685605241063E-5 0.0 4.068468788133172 0.0016010523673939195 86-87 0.0 4.3271685605241063E-5 0.0 4.349366935239596 0.0016443240529991606 88-89 0.0 4.3271685605241063E-5 0.0 4.650667682108889 0.0016875957386044017 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6835 0.0 30.75666 1 GTATCAA 9455 0.0 28.169683 1 ATCAACG 11180 0.0 23.606997 3 TCAACGC 11225 0.0 23.553703 4 CAACGCA 11260 0.0 23.48049 5 TATCAAC 11270 0.0 23.464231 2 AACGCAG 11695 0.0 22.660955 6 GTACATG 6265 0.0 22.091608 1 GAGTACT 7595 0.0 21.918182 12-13 TACATGG 6315 0.0 21.314175 2 GTACTTT 7785 0.0 20.986696 14-15 GGACCGA 865 0.0 20.864681 6 TAAGGTG 1300 0.0 20.84982 5 ACATGGG 6420 0.0 20.443895 3 ACGCAGA 13035 0.0 20.258535 7 CGCAGAG 13035 0.0 20.185663 8 AGAGTAC 10580 0.0 19.056198 10-11 TAGACCG 125 0.005994784 19.020885 5 AGTACTT 7880 0.0 18.654287 12-13 AGGACCG 1160 0.0 18.446981 5 >>END_MODULE