FastQCFastQC Report
Thu 26 May 2016
SRR938094_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938094_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2263285
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73760.32589797572996776No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61300.27084525369098456No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37640.1663069388079716No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT24490.10820555078127587No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA23200.1025058708912046No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA93750.029.687131
GGTATCA67550.029.5407711
GTACATG64850.025.3232461
TACATGG63050.025.1029032
GTAAGGT11250.024.9277764
ATCAACG110550.024.8085333
TATCAAC111500.024.7665822
TCAACGC111100.024.6429394
CAACGCA111350.024.544925
AACGCAG115550.023.6897096
ACATGGG64550.023.3424823
TAAGGTG14100.021.9118125
GAGTACT69700.021.81051612-13
CGCAGAG128350.020.9874638
ACGCAGA128800.020.9510237
GTGTAAG9100.020.459461
AGAGTAC98650.020.24853710-11
GTACTTT73850.020.19846214-15
CATGGGG41300.019.9104044
AGTACTT73300.019.08666612-13
Produced by FastQC (version 0.11.5)