##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938094_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2263285 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.592962441760537 33.0 31.0 34.0 30.0 34.0 2 31.804208042734345 33.0 31.0 34.0 30.0 34.0 3 31.882093063843044 34.0 31.0 34.0 30.0 34.0 4 35.41580578672151 37.0 35.0 37.0 33.0 37.0 5 35.408826108952255 37.0 35.0 37.0 33.0 37.0 6 35.42128719980029 37.0 35.0 37.0 33.0 37.0 7 35.35345261423108 37.0 35.0 37.0 33.0 37.0 8 35.31644092546895 37.0 35.0 37.0 33.0 37.0 9 36.91488743132217 39.0 37.0 39.0 33.0 39.0 10-11 36.81151003961057 39.0 37.0 39.0 33.0 39.0 12-13 36.69547913762518 39.0 37.0 39.0 32.0 39.0 14-15 37.82417304935083 40.0 38.0 41.0 32.5 41.0 16-17 37.65347315958883 40.0 38.0 41.0 32.0 41.0 18-19 37.79367622725375 40.0 38.0 41.0 32.0 41.0 20-21 37.759081158581445 40.0 38.0 41.0 32.0 41.0 22-23 37.660618083891336 40.0 38.0 41.0 32.0 41.0 24-25 37.540361244827764 40.0 37.5 41.0 32.0 41.0 26-27 37.34949045303618 40.0 37.0 41.0 31.5 41.0 28-29 37.12480244423482 40.0 37.0 41.0 31.0 41.0 30-31 36.904262830354995 40.0 36.5 41.0 30.0 41.0 32-33 36.640488051659425 39.0 36.0 41.0 30.0 41.0 34-35 36.38783052068122 39.0 35.5 41.0 30.0 41.0 36-37 36.10813397340591 39.0 35.0 40.5 29.5 41.0 38-39 35.7899416114188 39.0 35.0 40.0 27.5 41.0 40-41 35.57148679905536 38.5 35.0 40.0 27.0 41.0 42-43 35.38178068603821 38.5 35.0 40.0 26.0 41.0 44-45 35.09622561895652 38.0 34.5 40.0 25.0 41.0 46-47 34.71516136942542 38.0 34.0 40.0 24.0 41.0 48-49 34.32407739193252 38.0 33.5 40.0 23.0 41.0 50-51 33.64309532383239 37.5 33.0 39.5 22.0 40.5 52-53 33.805689075834465 38.0 33.0 39.5 22.0 40.5 54-55 33.89693255599715 38.0 33.0 40.0 21.5 41.0 56-57 33.568141440428406 38.0 33.0 40.0 19.5 41.0 58-59 33.225170051495944 37.5 33.0 40.0 17.5 41.0 60-61 32.76447950655795 37.0 32.0 40.0 13.5 41.0 62-63 32.188787757617796 36.0 31.0 39.0 8.0 41.0 64-65 31.626106301239126 35.5 31.0 39.0 6.5 40.0 66-67 31.039856226679362 35.0 30.0 38.5 2.0 40.0 68-69 30.383738238887283 35.0 29.0 38.0 2.0 40.0 70-71 29.78020023991676 34.5 28.5 37.0 2.0 39.5 72-73 29.091370728829997 34.0 27.5 36.5 2.0 39.0 74-75 28.35082302935777 34.0 26.0 36.0 2.0 39.0 76-77 27.628840601161585 33.5 25.5 35.5 2.0 38.0 78-79 26.845706793444045 33.0 24.5 35.0 2.0 37.0 80-81 26.127640796452944 32.0 22.0 35.0 2.0 36.5 82-83 25.346932003702584 31.5 19.0 35.0 2.0 36.0 84-85 24.63620555961799 31.0 16.0 34.0 2.0 35.5 86-87 23.899807580574254 31.0 7.5 34.0 2.0 35.0 88-89 23.02640056378229 30.0 2.0 34.0 2.0 35.0 90-91 22.139897317394848 29.5 2.0 34.0 2.0 35.0 92-93 21.19098368080025 29.0 2.0 34.0 2.0 35.0 94-95 20.185139078816853 28.0 2.0 34.0 2.0 35.0 96-97 18.939609903304266 25.5 2.0 33.0 2.0 35.0 98-99 17.430194827430043 21.5 2.0 32.5 2.0 34.0 100-101 14.01551660528833 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4513749043909776 1101 2 0.5412689439256617 1101 3 0.402011506054226 1101 4 0.3047374068950077 1101 5 0.31245129832387164 1101 6 0.33741823017945904 1101 7 0.3894738442863712 1101 8 0.39497437407018055 1101 9 0.45686189227230045 1101 10-11 0.44694410428403586 1101 12-13 0.49201211085070895 1101 14-15 0.6022488099622976 1101 16-17 0.7013194041815325 1101 18-19 0.5959082435344385 1101 20-21 0.7279259882199227 1101 22-23 0.6690757661003417 1101 24-25 0.6973652151836731 1101 26-27 0.8860896063693247 1101 28-29 0.8331427297025868 1101 30-31 0.8199001892632367 1101 32-33 0.83641663111041 1101 34-35 0.9566069324312068 1101 36-37 1.1116719758457663 1101 38-39 1.188885417453939 1101 40-41 1.270786242087972 1101 42-43 1.3513662440179885 1101 44-45 1.3692065092759975 1101 46-47 1.432178440446016 1101 48-49 1.5480813164361393 1101 50-51 1.4479309149115736 1101 52-53 1.4658769276635368 1101 54-55 1.5441322636471924 1101 56-57 1.50547298444814 1101 58-59 1.5969015991429671 1101 60-61 1.4884046598261733 1101 62-63 1.6261196790025636 1101 64-65 1.8022196921468492 1101 66-67 1.8920813728353245 1101 68-69 2.247278391187418 1101 70-71 2.4430099414367383 1101 72-73 2.5384393877634963 1101 74-75 2.7200229251849812 1101 76-77 2.661319265526558 1101 78-79 2.9213935432978424 1101 80-81 3.17131479810001 1101 82-83 3.4283347867223277 1101 84-85 3.5066699928698064 1101 86-87 3.6512334027492095 1101 88-89 4.08823511865285 1101 90-91 4.330817889763775 1101 92-93 4.746689864197343 1101 94-95 4.971827524553898 1101 96-97 5.222408295624707 1101 98-99 5.284236384758103 1101 100-101 5.189527927492984 1102 1 0.4840105211603323 1102 2 0.41920597214949495 1102 3 0.3057435608314769 1102 4 0.296695215594859 1102 5 0.2824401621739341 1102 6 0.38729321113769544 1102 7 0.23086250117213325 1102 8 0.2822129765884185 1102 9 0.43761395009623527 1102 10-11 0.46782537033821825 1102 12-13 0.5616094365988502 1102 14-15 0.5445749126775254 1102 16-17 0.6533174107056396 1102 18-19 0.6037337526718645 1102 20-21 0.6210764024111555 1102 22-23 0.6606254900604043 1102 24-25 0.585961035975707 1102 26-27 0.6262584740014816 1102 28-29 0.6456726055765429 1102 30-31 0.7180835391432083 1102 32-33 0.7237451895568157 1102 34-35 0.8583694776718147 1102 36-37 0.9397369778681295 1102 38-39 0.8999397534609912 1102 40-41 1.0173212973222263 1102 42-43 1.2273143926330548 1102 44-45 1.295084794308913 1102 46-47 1.2883773231539948 1102 48-49 1.42499370067533 1102 50-51 1.3836866235016458 1102 52-53 1.3572141061203453 1102 54-55 1.65285089726261 1102 56-57 1.6240578792062692 1102 58-59 1.5311825558012941 1102 60-61 1.5090180279337133 1102 62-63 1.5503577457249094 1102 64-65 1.653610200680177 1102 66-67 1.6974052535734607 1102 68-69 1.7346261227221582 1102 70-71 1.940023667266832 1102 72-73 2.2920159566736764 1102 74-75 2.285733760868986 1102 76-77 2.5210238029823664 1102 78-79 2.7682852705041903 1102 80-81 2.935414897379836 1102 82-83 3.2436585492701937 1102 84-85 3.3004351934518255 1102 86-87 3.368454644429928 1102 88-89 3.7326705237303024 1102 90-91 4.001146484980481 1102 92-93 4.286172965615101 1102 94-95 4.5432676336041204 1102 96-97 4.756184120198828 1102 98-99 4.776588489332852 1102 100-101 4.530191684052545 1103 1 0.6894277924331682 1103 2 0.5467022474519645 1103 3 0.5028742900310057 1103 4 0.5037576627170992 1103 5 0.3874062637865947 1103 6 0.4749809221432244 1103 7 0.4639460654286296 1103 8 0.5480010837626494 1103 9 0.6344632037817064 1103 10-11 0.6095082542004491 1103 12-13 0.622114135136961 1103 14-15 0.8178419374975192 1103 16-17 0.8337411931218384 1103 18-19 0.8663843784695757 1103 20-21 0.8749094841959746 1103 22-23 0.9087348319738808 1103 24-25 0.9487113914598666 1103 26-27 1.0402753884151252 1103 28-29 1.175306396226695 1103 30-31 1.0657474541440735 1103 32-33 1.0757849164285034 1103 34-35 1.1085150684549419 1103 36-37 1.233379432787558 1103 38-39 1.2957711658048296 1103 40-41 1.4052402550216314 1103 42-43 1.4430536460111654 1103 44-45 1.4016232763794036 1103 46-47 1.6023855021206543 1103 48-49 1.6790564923624203 1103 50-51 1.5287176909570874 1103 52-53 1.5253289966603418 1103 54-55 1.772167397066056 1103 56-57 1.9077140196593163 1103 58-59 1.8969514935224083 1103 60-61 1.8126103996237433 1103 62-63 1.972913155150458 1103 64-65 1.960434283336923 1103 66-67 2.202195190338532 1103 68-69 2.3507784495055226 1103 70-71 2.588306262212239 1103 72-73 2.7272296758549395 1103 74-75 2.844628467996948 1103 76-77 2.955048935186884 1103 78-79 3.199831719625873 1103 80-81 3.214881663048235 1103 82-83 3.248698821298486 1103 84-85 3.398109218923743 1103 86-87 3.5715012124507517 1103 88-89 3.8313479123493117 1103 90-91 3.996559566474115 1103 92-93 4.310482582209858 1103 94-95 4.489940156255617 1103 96-97 4.928561989752723 1103 98-99 5.173270798920328 1103 100-101 4.727538372913719 1104 1 0.4397309046184539 1104 2 0.42649443483859173 1104 3 0.44770987387536465 1104 4 0.38974394698453807 1104 5 0.3642581503878546 1104 6 0.3523649751647113 1104 7 0.2567807085368585 1104 8 0.3872765668281488 1104 9 0.4646770961524851 1104 10-11 0.45994334465873266 1104 12-13 0.42473906414390683 1104 14-15 0.5500297494603785 1104 16-17 0.6803810638206542 1104 18-19 0.657275000381226 1104 20-21 0.7294930708525342 1104 22-23 0.7020188266997565 1104 24-25 0.565390067523893 1104 26-27 0.6295291044479256 1104 28-29 0.7455414801798881 1104 30-31 0.7390899696624587 1104 32-33 0.7593071475743685 1104 34-35 0.6901635834179061 1104 36-37 0.9440719246624525 1104 38-39 1.0502365349561913 1104 40-41 1.1282994238212183 1104 42-43 1.0649413981772398 1104 44-45 1.2648199777982612 1104 46-47 1.3500656868652143 1104 48-49 1.492829644854254 1104 50-51 1.5327128171433273 1104 52-53 1.3813314435750499 1104 54-55 1.6826438448864778 1104 56-57 1.7353894256645148 1104 58-59 1.7949720696231992 1104 60-61 1.9840007625827596 1104 62-63 2.0307101312842875 1104 64-65 2.1679472872624928 1104 66-67 2.2187386355844403 1104 68-69 2.4892623131288154 1104 70-71 2.4469128718843116 1104 72-73 2.681700048921865 1104 74-75 2.7920223036733667 1104 76-77 2.6645662574915896 1104 78-79 3.039287593847451 1104 80-81 3.231663169577093 1104 82-83 3.3744956323030912 1104 84-85 3.4509904735054526 1104 86-87 3.409805185309512 1104 88-89 3.577548063967953 1104 90-91 3.785735346522003 1104 92-93 4.100021146136129 1104 94-95 4.442826289655006 1104 96-97 4.5940144563078 1104 98-99 4.762161856172629 1104 100-101 4.362540512087621 1105 1 0.42466273635879404 1105 2 0.3486094843980929 1105 3 0.1925993068373124 1105 4 0.13499479122010882 1105 5 0.27335619117752685 1105 6 0.3366031745666831 1105 7 0.40331482733006396 1105 8 0.3591481518017545 1105 9 0.49954954406164376 1105 10-11 0.4751802688134532 1105 12-13 0.40205143039346325 1105 14-15 0.5692097235175737 1105 16-17 0.6206275204029552 1105 18-19 0.5211648876158037 1105 20-21 0.4654637091511802 1105 22-23 0.47827341275522883 1105 24-25 0.5146435279319306 1105 26-27 0.4191751549214473 1105 28-29 0.5951752342779244 1105 30-31 0.5928652670833259 1105 32-33 0.6672240795374691 1105 34-35 0.6224410217581493 1105 36-37 0.566453517316738 1105 38-39 0.6064974698820933 1105 40-41 0.7545138733394907 1105 42-43 0.8224270448042148 1105 44-45 0.8438748170787918 1105 46-47 0.8461225457433272 1105 48-49 1.0310043263617032 1105 50-51 0.9829342066337574 1105 52-53 1.0001486380675928 1105 54-55 1.052107602505579 1105 56-57 1.1940835469388915 1105 58-59 1.1000310976987109 1105 60-61 1.3537344384145413 1105 62-63 1.196521190359789 1105 64-65 1.5118608550540173 1105 66-67 1.7947647170360703 1105 68-69 1.9228748386474415 1105 70-71 1.8601239043237534 1105 72-73 2.039752532917422 1105 74-75 2.1906460765443505 1105 76-77 2.3032084384380447 1105 78-79 2.5126242536483367 1105 80-81 2.6423334679922483 1105 82-83 2.7443653162643535 1105 84-85 2.9993852856755794 1105 86-87 3.029978721101269 1105 88-89 3.4483772848265453 1105 90-91 3.5534914769219625 1105 92-93 3.5220548279883985 1105 94-95 3.762984683025909 1105 96-97 3.8587483966758533 1105 98-99 4.067970434080383 1105 100-101 3.8330667020903366 1106 1 0.18012069864117208 1106 2 0.15840056307389716 1106 3 0.20472852750296155 1106 4 0.24579266342789907 1106 5 0.18770054997404628 1106 6 0.10708947974520555 1106 7 0.20301695351927407 1106 8 0.14561088783077736 1106 9 0.1036847962979266 1106 10-11 0.19297422149775656 1106 12-13 0.37115272209427985 1106 14-15 0.5489514902206167 1106 16-17 0.19254354506642812 1106 18-19 0.3828236621582377 1106 20-21 0.2663229372369571 1106 22-23 0.27278795004449563 1106 24-25 0.2674560879332688 1106 26-27 0.33974932254368184 1106 28-29 0.3338226691683417 1106 30-31 0.3048912542756739 1106 32-33 0.3591544493078018 1106 34-35 0.4613745394725939 1106 36-37 0.5313365033502961 1106 38-39 0.2623254233550725 1106 40-41 0.679039624533992 1106 42-43 0.5735101413381969 1106 44-45 0.6572301756904366 1106 46-47 0.8227780839983083 1106 48-49 0.9837734983815167 1106 50-51 0.913246268813225 1106 52-53 0.8270657688039833 1106 54-55 0.3521317544361864 1106 56-57 0.7720895628356956 1106 58-59 0.9730229341324304 1106 60-61 1.2346560696744078 1106 62-63 0.9467007268350827 1106 64-65 0.9465747668866413 1106 66-67 1.0496771235408424 1106 68-69 0.8587819199721203 1106 70-71 1.051195865321592 1106 72-73 1.2773301405867628 1106 74-75 1.519540423959544 1106 76-77 1.6429374024805234 1106 78-79 1.8833187178334825 1106 80-81 1.8757868129099577 1106 82-83 2.057862362639977 1106 84-85 2.3983609303415463 1106 86-87 2.2216347990343515 1106 88-89 2.4296370074925626 1106 90-91 2.6126751060452023 1106 92-93 2.954896787087563 1106 94-95 2.7910903345420444 1106 96-97 3.355608912864593 1106 98-99 3.524001897847679 1106 100-101 3.4658867356536884 1107 1 0.24426146152212524 1107 2 0.11193578651581149 1107 3 0.15892865535308331 1107 4 0.12772134693287995 1107 5 0.10734187854729527 1107 6 0.17063235220554418 1107 7 0.271568074459104 1107 8 0.1345404912074386 1107 9 0.26253935971488573 1107 10-11 0.18819162167962844 1107 12-13 0.19298530335266406 1107 14-15 0.32439416211018113 1107 16-17 0.40338054453606986 1107 18-19 0.31805909329116133 1107 20-21 0.3622594812732558 1107 22-23 0.3591092957272437 1107 24-25 0.34653154620685456 1107 26-27 0.40420133398421854 1107 28-29 0.4360006761199955 1107 30-31 0.5600221435879433 1107 32-33 0.4722185644168917 1107 34-35 0.5128072514082547 1107 36-37 0.5990682735823682 1107 38-39 0.6595021449993865 1107 40-41 0.5865393797758784 1107 42-43 0.7009128938330278 1107 44-45 0.6722572770894075 1107 46-47 0.7351091289086753 1107 48-49 0.8197797997905383 1107 50-51 0.7305438878795414 1107 52-53 0.6996271533548466 1107 54-55 0.836372996414795 1107 56-57 1.0505284929364436 1107 58-59 0.8434676190593677 1107 60-61 0.9175037348390234 1107 62-63 1.107243205064698 1107 64-65 1.2291075710189006 1107 66-67 1.4625448038987834 1107 68-69 1.5611924340479355 1107 70-71 1.6957494867208354 1107 72-73 1.9048700623374621 1107 74-75 1.9068302264724615 1107 76-77 2.2995873104148252 1107 78-79 2.4391670232958056 1107 80-81 2.5846979455562966 1107 82-83 2.6120266798904765 1107 84-85 2.7307264874555273 1107 86-87 2.868462445864349 1107 88-89 3.2857592849838717 1107 90-91 3.2862311247651412 1107 92-93 3.6317629303121635 1107 94-95 4.109284527465892 1107 96-97 4.26769441433602 1107 98-99 4.289671783803485 1107 100-101 4.162926445106168 1108 1 0.17231697277323832 1108 2 0.1193754438652519 1108 3 0.07492892782540395 1108 4 0.11944533247190492 1108 5 -0.015193077657762899 1108 6 0.07583341684129152 1108 7 0.09622598878316779 1108 8 0.12849044269407983 1108 9 0.15310600245715023 1108 10-11 0.048660456827228415 1108 12-13 0.12370913271853823 1108 14-15 0.28250735774530966 1108 16-17 0.2797959053292942 1108 18-19 0.19021400045119918 1108 20-21 0.28644281194832644 1108 22-23 0.2617036808037412 1108 24-25 0.29152146140815205 1108 26-27 0.2246777119631389 1108 28-29 0.33562702352885054 1108 30-31 0.35413134185475315 1108 32-33 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-3.5742865918551203 2316 92-93 -4.072285773629055 2316 94-95 -4.08687021813261 2316 96-97 -4.331151462227918 2316 98-99 -4.1318936253203695 2316 100-101 -3.9947694622269747 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9083.0 3 2970.0 4 3170.0 5 3415.0 6 3666.0 7 4629.0 8 5518.0 9 7033.0 10 8408.0 11 10417.0 12 12719.0 13 14741.0 14 16976.0 15 19685.0 16 22680.0 17 26666.0 18 26638.0 19 24203.0 20 25455.0 21 27467.0 22 30085.0 23 33152.0 24 37237.0 25 41612.0 26 47022.0 27 53148.0 28 61095.0 29 70443.0 30 81218.0 31 96729.0 32 116447.0 33 143039.0 34 182610.0 35 235895.0 36 295153.0 37 308979.0 38 146540.0 39 7342.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.52182236293419 18.49047508929257 14.38756637299727 23.600136174775976 2 15.458179587232607 19.501738870609287 40.54605134044205 24.494030201716065 3 19.527955241906252 23.352648582123994 29.412328987549706 27.707067188420048 4 12.805153272364203 16.34995108352317 36.875817577129105 33.96907806698352 5 13.80864446472665 37.12351155731964 34.340265248798985 14.727578729154727 6 32.866969596739814 35.807625843036476 16.982862459252132 14.342542100971581 7 28.844306625969452 32.42810760155333 20.291570062349845 18.436015710127375 8 26.271318668932704 34.95300334380159 19.438692182399624 19.33698580486608 9 25.981359225017624 16.90564019370139 20.118422186810005 36.99457839447098 10-11 25.09842206422432 26.225822165718622 27.08777223547385 21.58798353458321 12-13 26.028998563178064 23.543303240595957 27.425993503469613 23.00170469275637 14-15 22.751618122440934 25.141049092753054 25.78069559540519 26.326637189400827 16-17 22.351547307882964 28.23946558334746 26.641758339873135 22.767228768896445 18-19 22.30051508852428 26.956225763709078 28.660802989966772 22.082456157799875 20-21 22.697663479718962 26.091238922052185 29.088070541666166 22.123027056562687 22-23 22.51277228477747 25.959471272167082 28.9215362326208 22.60622021043465 24-25 22.494510501174165 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27.889192168204524 22.82449880097619 50-51 22.627263553814565 26.998634305684412 27.92675848333471 22.447343657166314 52-53 22.838457336991105 26.830513702504348 27.69114039057087 22.63988856993368 54-55 22.49676859137098 26.86205365132604 27.893718141625186 22.7474596156778 56-57 22.608965210807003 26.97378232963618 27.768582587793812 22.648669871763005 58-59 22.711121343512314 26.640260295789066 27.73429007266576 22.914328288032863 60-61 22.42177227283705 26.871688563750045 27.93344724338917 22.773091920023738 62-63 22.356244010834576 26.902055091594324 28.107212717258808 22.6344881803123 64-65 22.156709507342175 26.976324168844716 27.924479858872576 22.942486464940533 66-67 22.264762312126674 26.868150417083868 27.770609616745922 23.096477654043536 68-69 22.48632960386176 26.62513055065365 27.940944153816343 22.947595691668244 70-71 22.437008175005843 26.685235980985233 27.66438947302831 23.213366370980605 72-73 22.44101029875447 26.61800481241949 27.782331924371217 23.158652964454824 74-75 22.307213868515564 27.245243248080232 27.43002274668107 23.017520136723128 76-77 22.65554109882768 26.686118072507963 27.54600984368396 23.112330984980396 78-79 22.59186502048957 26.78432271069061 27.60182865031316 23.021983618506656 80-81 22.768725971099034 26.831455212338152 27.329532900260727 23.07028591630209 82-83 22.72228128924606 26.745430554413986 27.426262701251368 23.106025455088588 84-85 22.649672735885577 26.930288236344257 27.346372344017066 23.0736666837531 86-87 22.819528856074214 26.82319178690491 27.21047271249086 23.146806644530017 88-89 22.692079486882015 26.901945185651698 27.02830214738331 23.37767318008298 90-91 22.77134885776636 26.740189497527695 27.071340570702468 23.41712107400348 92-93 22.87440836256766 26.426608234103817 27.302345426331375 23.39663797699715 94-95 22.663448755029737 26.331156432758878 27.35175536917735 23.653639443034034 96-97 22.799178573614697 26.093590721850646 27.314526620229955 23.792704084304706 98-99 22.715053971344773 26.260198693745185 26.973850375774205 24.05089695913584 100-101 23.56299968671402 25.12909771867627 27.068815832092874 24.239086762516838 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 535.0 1 367.5 2 284.0 3 404.5 4 359.5 5 304.0 6 546.0 7 1220.5 8 2056.0 9 2412.5 10 2036.5 11 1646.5 12 1662.0 13 1781.5 14 1933.0 15 2088.5 16 2228.0 17 2398.5 18 2630.5 19 2944.5 20 3390.0 21 4095.0 22 5020.5 23 6241.5 24 7978.0 25 10205.0 26 12981.0 27 16261.5 28 19274.5 29 22196.0 30 25215.5 31 29003.5 32 34688.0 33 37865.5 34 39532.5 35 43024.5 36 47770.5 37 52398.0 38 55388.0 39 58147.5 40 61678.0 41 66442.0 42 69885.0 43 72378.0 44 75803.0 45 79327.5 46 81888.5 47 85641.5 48 89160.0 49 88221.0 50 85422.5 51 82465.0 52 80333.0 53 80760.0 54 81691.0 55 77921.5 56 71137.5 57 65365.0 58 60200.0 59 52547.5 60 42588.0 61 35086.5 62 29025.5 63 22373.5 64 16893.5 65 13081.0 66 9737.0 67 7152.5 68 5336.0 69 3853.5 70 2791.0 71 2004.5 72 1418.5 73 1082.0 74 800.5 75 557.0 76 368.5 77 218.0 78 130.5 79 78.0 80 51.5 81 34.5 82 27.5 83 18.5 84 14.0 85 10.0 86 7.0 87 7.5 88 4.0 89 1.5 90 2.5 91 3.5 92 3.0 93 3.0 94 3.0 95 1.5 96 0.5 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.45500235277483836 2 0.03963265784026316 3 0.0516505875309561 4 0.05593639333976941 5 0.08222561453816024 6 0.0802815376764305 7 0.046392743291277946 8 0.03932337288498797 9 0.04595090764088482 10-11 0.04811590232781113 12-13 0.04420565682183198 14-15 0.03815250841144619 16-17 0.037799039891131696 18-19 0.04650320220387622 20-21 0.055052722038983155 22-23 0.046945037854269345 24-25 0.06479519813015153 26-27 0.05611312759992666 28-29 0.04433820751694992 30-31 0.045862540510806196 32-33 0.044956777427500295 34-35 0.049375133931431524 36-37 0.044514941777107166 38-39 0.026598506153666022 40-41 0.02423468542406281 42-43 0.024190501859023498 44-45 0.014072465465020976 46-47 0.026576414371146367 48-49 0.026996158239019834 50-51 0.014735218940610662 52-53 0.015066595678405502 54-55 0.01502241211336619 56-57 0.014425933985335475 58-59 0.014182924377619257 60-61 0.01716531501777284 62-63 0.015221238156043097 64-65 0.016149093021868656 66-67 0.008439060922508654 68-69 0.012128388603291233 70-71 0.018203628796196678 72-73 0.017231590365331808 74-75 0.014028281899981666 76-77 0.009256456875735933 78-79 0.013453895554470604 80-81 0.017010672540135247 82-83 0.014094557247540631 84-85 0.012415581776046763 86-87 0.003092849552751863 88-89 0.02860885836295473 90-91 0.031922625740903154 92-93 0.017297865712890773 94-95 0.032740021694130435 96-97 0.028078655582482983 98-99 0.02458815394437731 100-101 0.14885443061744322 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2263285.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.140305659380104 #Duplication Level Percentage of deduplicated Percentage of total 1 75.0586132671375 40.63696264652027 2 13.71672877122293 14.852557766416455 3 4.801994850227482 7.799444068982552 4 2.177149338621545 4.7148612263635075 5 1.1578133123675944 3.134218331404045 6 0.6996745791671048 2.272835734692312 7 0.46359032758826885 1.7569245425452722 8 0.3104128923413202 1.3444679097577061 9 0.24306308073291388 1.1843558536851488 >10 1.250472436311852 12.221484519112847 >50 0.07686266623809669 2.826041319386578 >100 0.03837058117596776 3.839408392948017 >500 0.0035299619578278013 1.405393281272182 >1k 0.0015597506325284603 1.4107711183751148 >5k 1.64184277108259E-4 0.6002732885380349 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7376 0.32589797572996776 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6130 0.27084525369098456 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3764 0.1663069388079716 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2449 0.10820555078127587 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2320 0.1025058708912046 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.418356503931233E-5 2 0.0 0.0 0.0 0.0 4.418356503931233E-5 3 0.0 0.0 0.0 0.0 4.418356503931233E-5 4 0.0 0.0 0.0 0.0 4.418356503931233E-5 5 0.0 0.0 0.0 0.0 4.418356503931233E-5 6 0.0 0.0 0.0 0.0 4.418356503931233E-5 7 0.0 0.0 0.0 0.0 4.418356503931233E-5 8 0.0 0.0 0.0 0.0 4.418356503931233E-5 9 0.0 0.0 0.0 8.836713007862466E-5 4.418356503931233E-5 10-11 0.0 0.0 0.0 1.1045891259828082E-4 8.836713007862466E-5 12-13 0.0 0.0 0.0 1.3255069511793698E-4 1.7673426015724932E-4 14-15 0.0 0.0 0.0 1.3255069511793698E-4 2.2091782519656163E-4 16-17 0.0 0.0 0.0 1.7673426015724932E-4 2.6510139023587395E-4 18-19 0.0 0.0 0.0 2.871931727555301E-4 2.6510139023587395E-4 20-21 0.0 0.0 0.0 3.5346852031449864E-4 3.0928495527518627E-4 22-23 0.0 0.0 0.0 4.197438678734671E-4 3.0928495527518627E-4 24-25 0.0 0.0 0.0 5.743863455110602E-4 3.0928495527518627E-4 26-27 0.0 0.0 0.0 0.0013255069511793698 3.0928495527518627E-4 28-29 0.0 0.0 0.0 0.004175346896215015 3.0928495527518627E-4 30-31 0.0 0.0 0.0 0.01405037368250132 3.0928495527518627E-4 32-33 0.0 0.0 0.0 0.035302668466410544 3.0928495527518627E-4 34-35 0.0 0.0 0.0 0.06616488864637021 3.5346852031449864E-4 36-37 0.0 0.0 0.0 0.11447961701685824 3.5346852031449864E-4 38-39 0.0 0.0 0.0 0.18645464446589802 3.5346852031449864E-4 40-41 0.0 0.0 0.0 0.26247246811603486 3.5346852031449864E-4 42-43 0.0 0.0 0.0 0.3394181466319973 3.5346852031449864E-4 44-45 0.0 0.0 0.0 0.4383672405375373 3.755603028341548E-4 46-47 0.0 0.0 0.0 0.5486052353106214 3.9765208535381095E-4 48-49 0.0 0.0 0.0 0.6601466452523654 3.9765208535381095E-4 50-51 0.0 0.0 0.0 0.7777853871695346 3.9765208535381095E-4 52-53 0.0 0.0 0.0 0.8903651108897024 4.860192154324356E-4 54-55 0.0 0.0 0.0 1.0113838955323788 5.743863455110603E-4 56-57 0.0 0.0 0.0 1.155797877863371 5.964781280307163E-4 58-59 0.0 0.0 0.0 1.3038349125275872 6.185699105503725E-4 60-61 0.0 0.0 0.0 1.463823601534937 6.627534755896849E-4 62-63 0.0 0.0 0.0 1.61654409409332 6.627534755896849E-4 64-65 0.0 0.0 0.0 1.7726667211597302 7.069370406289973E-4 66-67 0.0 0.0 0.0 1.9570005545037412 7.511206056683096E-4 68-69 0.0 0.0 0.0 2.1572625630444247 7.953041707076219E-4 70-71 0.0 0.0 0.0 2.3625614979995895 7.953041707076219E-4 72-73 0.0 0.0 0.0 2.566048906788142 8.394877357469342E-4 74-75 2.2091782519656165E-5 0.0 0.0 2.777356806588653 8.836713007862465E-4 76-77 4.418356503931233E-5 0.0 0.0 3.0022732444212723 8.836713007862465E-4 78-79 4.418356503931233E-5 0.0 0.0 3.2526835992815752 8.836713007862465E-4 80-81 4.418356503931233E-5 0.0 0.0 3.5149130577898937 9.057630833059027E-4 82-83 4.418356503931233E-5 0.0 0.0 3.78178179062734 0.0010162219959041834 84-85 4.418356503931233E-5 0.0 0.0 4.056603565171863 0.0010162219959041834 86-87 4.418356503931233E-5 0.0 0.0 4.3319997260618965 0.0010604055609434958 88-89 4.418356503931233E-5 0.0 0.0 4.62862608995332 0.0011045891259828082 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 9375 0.0 29.68713 1 GGTATCA 6755 0.0 29.540771 1 GTACATG 6485 0.0 25.323246 1 TACATGG 6305 0.0 25.102903 2 GTAAGGT 1125 0.0 24.927776 4 ATCAACG 11055 0.0 24.808533 3 TATCAAC 11150 0.0 24.766582 2 TCAACGC 11110 0.0 24.642939 4 CAACGCA 11135 0.0 24.54492 5 AACGCAG 11555 0.0 23.689709 6 ACATGGG 6455 0.0 23.342482 3 TAAGGTG 1410 0.0 21.911812 5 GAGTACT 6970 0.0 21.810516 12-13 CGCAGAG 12835 0.0 20.987463 8 ACGCAGA 12880 0.0 20.951023 7 GTGTAAG 910 0.0 20.45946 1 AGAGTAC 9865 0.0 20.248537 10-11 GTACTTT 7385 0.0 20.198462 14-15 CATGGGG 4130 0.0 19.910404 4 AGTACTT 7330 0.0 19.086666 12-13 >>END_MODULE