FastQCFastQC Report
Thu 26 May 2016
SRR938094_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938094_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2263285
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74320.3283722553721692No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63910.2823771641662451No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41290.18243394004732058No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT25130.11103329894379188No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA23630.10440576418789503No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65500.032.1999551
TAGATCG1102.7363694E-630.2253255
GTATCAA92650.029.27551
TCAACGC110500.024.6296354
ATCAACG109900.024.5058753
CAACGCA111800.024.300765
TATCAAC112550.024.1836992
AACGCAG116000.023.5027966
GTAAGGT11400.023.3318314
GTACATG62750.023.1644131
GAGTACT76500.022.32045712-13
TACATGG62200.022.2236942
TAAGGTG13350.022.0585025
TATACCG1100.002879295321.5895185
TTACTCG1553.9105173E-521.450234
GTACTTT79600.021.39152314-15
CGCAGAG128050.021.1798158
ACGCAGA129300.020.975067
ACATGGG61400.020.6551193
AGAGTAC103850.020.51264210-11
Produced by FastQC (version 0.11.5)