##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938094_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2263285 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.48479356333824 33.0 31.0 34.0 31.0 34.0 2 32.71908000980875 34.0 31.0 34.0 31.0 34.0 3 32.687841345654654 34.0 31.0 34.0 31.0 34.0 4 36.21329350921338 37.0 35.0 37.0 35.0 37.0 5 36.172640211020706 37.0 35.0 37.0 35.0 37.0 6 36.22952478366622 37.0 37.0 37.0 35.0 37.0 7 36.1887035879264 37.0 37.0 37.0 35.0 37.0 8 36.180853052090214 37.0 37.0 37.0 35.0 37.0 9 37.887947386210755 39.0 38.0 39.0 35.0 39.0 10-11 37.847616407125045 39.0 38.0 39.0 35.0 39.0 12-13 37.793129676554216 39.0 38.0 39.0 35.0 39.0 14-15 39.08374795927159 40.0 38.0 41.0 36.0 41.0 16-17 39.020175099468254 40.0 38.0 41.0 36.0 41.0 18-19 39.002358076866145 40.0 38.5 41.0 36.0 41.0 20-21 38.99928356349289 40.0 38.5 41.0 35.5 41.0 22-23 39.02895481567721 40.0 39.0 41.0 35.0 41.0 24-25 38.9882248589992 40.0 38.5 41.0 35.0 41.0 26-27 38.82756656806367 40.0 38.0 41.0 35.0 41.0 28-29 38.75339495467871 40.0 38.0 41.0 35.0 41.0 30-31 38.553023370896724 40.0 38.0 41.0 34.0 41.0 32-33 38.36749569762536 40.0 38.0 41.0 34.0 41.0 34-35 38.17304007228431 40.0 38.0 41.0 34.0 41.0 36-37 38.10664520818191 40.0 38.0 41.0 34.0 41.0 38-39 38.065081507631604 40.0 38.0 41.0 33.0 41.0 40-41 37.952723143572285 40.0 38.0 41.0 33.0 41.0 42-43 37.776907238814374 40.0 37.5 41.0 33.0 41.0 44-45 37.55536046056948 40.0 37.0 41.0 33.0 41.0 46-47 37.2391411156792 40.0 37.0 41.0 32.0 41.0 48-49 36.89838950905431 40.0 36.5 41.0 31.0 41.0 50-51 36.613802062046986 39.5 36.0 41.0 30.5 41.0 52-53 36.165572166121365 39.0 35.0 40.5 29.5 41.0 54-55 35.86922526327882 39.0 35.0 40.0 29.0 41.0 56-57 35.60362238957975 38.5 35.0 40.0 28.0 41.0 58-59 35.22908051791975 38.0 34.5 40.0 27.5 41.0 60-61 34.80697260839885 38.0 34.0 40.0 26.5 41.0 62-63 34.382124434174216 37.0 34.0 40.0 26.0 41.0 64-65 33.93943228537281 36.5 33.0 39.0 26.0 41.0 66-67 33.422119176329986 36.0 33.0 39.0 24.5 40.0 68-69 32.88656532429633 35.5 32.0 38.5 22.5 40.0 70-71 32.290672186666725 35.0 31.5 38.0 21.5 40.0 72-73 31.811601720508023 35.0 31.0 37.0 20.0 39.0 74-75 31.24817798023669 34.5 30.5 36.5 18.5 39.0 76-77 30.043793865995667 33.5 29.5 35.0 18.0 37.5 78-79 30.73350307186236 34.0 30.5 36.0 18.0 38.0 80-81 30.54748783295078 34.0 30.5 35.5 17.5 37.0 82-83 30.086525780005612 34.0 30.0 35.0 10.5 37.0 84-85 29.575833578183925 34.0 29.5 35.0 7.0 36.0 86-87 29.04122680086688 34.0 29.0 35.0 2.0 36.0 88-89 28.42463587219462 33.5 28.5 35.0 2.0 35.5 90-91 27.8955328206567 33.0 27.0 35.0 2.0 35.0 92-93 27.255703104116364 33.0 26.0 35.0 2.0 35.0 94-95 26.512049962775347 32.0 25.0 34.5 2.0 35.0 96-97 25.689929681856242 32.0 23.5 34.0 2.0 35.0 98-99 24.676397802309474 32.0 10.5 34.0 2.0 35.0 100-101 21.160652105236416 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.09386378476456514 1101 2 0.06177549050732267 1101 3 0.19466132295620753 1101 4 0.06517880120839692 1101 5 0.034145926451323305 1101 6 0.07399249051412937 1101 7 0.11438684368734897 1101 8 0.12195661338376595 1101 9 0.05624990372228922 1101 10-11 0.15386423244037672 1101 12-13 0.14693984373725044 1101 14-15 0.24192336291397964 1101 16-17 0.26330063297574213 1101 18-19 0.27585117974981443 1101 20-21 0.24377770285263978 1101 22-23 0.1532631581403976 1101 24-25 0.18443012197115394 1101 26-27 0.31121563754891923 1101 28-29 0.3139126800052381 1101 30-31 0.3335985105209147 1101 32-33 0.31231677992258255 1101 34-35 0.36567750561925294 1101 36-37 0.37671504457128435 1101 38-39 0.44920428834777937 1101 40-41 0.3831160460252292 1101 42-43 0.3277415713675964 1101 44-45 0.33384685647732937 1101 46-47 0.3387709349930006 1101 48-49 0.3718102226547515 1101 50-51 0.3783431784207849 1101 52-53 0.33645249596865057 1101 54-55 0.3773023226361545 1101 56-57 0.5517460667254852 1101 58-59 0.3988793869048237 1101 60-61 0.5103752206533727 1101 62-63 0.5520262668977267 1101 64-65 0.4699148171900589 1101 66-67 0.7138773909862479 1101 68-69 0.6423424558829467 1101 70-71 0.6074715067250622 1101 72-73 0.5896601651343225 1101 74-75 0.8569147245247599 1101 76-77 0.9348587672639468 1101 78-79 0.9524571311518564 1101 80-81 0.9782026296266686 1101 82-83 1.2014590241429346 1101 84-85 1.1940805707999758 1101 86-87 1.387480871551361 1101 88-89 1.534750550340501 1101 90-91 1.7331576119216514 1101 92-93 1.9205284016651056 1101 94-95 2.0328919171169098 1101 96-97 2.2418382480374355 1101 98-99 2.5185137073080597 1101 100-101 2.938212004516359 1102 1 0.06357884115445245 1102 2 0.0695359949959311 1102 3 0.19761017020710625 1102 4 0.06898912420927417 1102 5 -0.004485876711974868 1102 6 -0.007783357698045279 1102 7 0.12096610208280367 1102 8 0.11777070796749456 1102 9 0.05256039744244134 1102 10-11 0.16973620085993701 1102 12-13 0.13344973757612166 1102 14-15 0.2171254026154017 1102 16-17 0.2252466489130498 1102 18-19 0.22330739682069378 1102 20-21 0.2733364329113144 1102 22-23 0.31824561345134583 1102 24-25 0.07827194924526282 1102 26-27 0.223332307104684 1102 28-29 0.06672232538189604 1102 30-31 0.13270065843209977 1102 32-33 0.19562489933151994 1102 34-35 0.04021104801803688 1102 36-37 0.15229897016919836 1102 38-39 0.23501856678304733 1102 40-41 0.2921390852486425 1102 42-43 0.3509137588331228 1102 44-45 0.2621877671202313 1102 46-47 0.2790178738649871 1102 48-49 0.4838152024048199 1102 50-51 0.5409979420139805 1102 52-53 0.49091371905409886 1102 54-55 0.5246511178833302 1102 56-57 0.73193862494076 1102 58-59 0.7827150945493742 1102 60-61 0.7476162526382879 1102 62-63 0.7764751719305281 1102 64-65 0.7822756153158252 1102 66-67 0.6762295218255048 1102 68-69 0.7745688060098033 1102 70-71 0.9988782861189804 1102 72-73 1.076674009154683 1102 74-75 1.046994883792717 1102 76-77 1.0402585524203012 1102 78-79 1.1854298528374052 1102 80-81 1.4313694066042508 1102 82-83 1.4219062035977892 1102 84-85 1.175195735399626 1102 86-87 1.2339385778196466 1102 88-89 1.451773757656742 1102 90-91 1.6529868532451708 1102 92-93 1.783719223796627 1102 94-95 1.9416280166278632 1102 96-97 1.9679158806600476 1102 98-99 2.237680163858691 1102 100-101 2.3548366479757092 1103 1 0.2688650807224704 1103 2 0.24726432582995272 1103 3 0.35716634479310017 1103 4 0.16025513312914086 1103 5 -0.012059832785617175 1103 6 0.07980810162308671 1103 7 0.15026543835059414 1103 8 0.13509924651103944 1103 9 0.22782339101541993 1103 10-11 0.24162731189554876 1103 12-13 0.2110201024104441 1103 14-15 0.3787400691073586 1103 16-17 0.377349788659167 1103 18-19 0.4326645164526397 1103 20-21 0.2917589244225596 1103 22-23 0.2886373011882384 1103 24-25 0.4328109845607315 1103 26-27 0.4102049523759774 1103 28-29 0.47549433183318257 1103 30-31 0.47347610729657674 1103 32-33 0.4968190672882855 1103 34-35 0.4606307215387986 1103 36-37 0.4978117813492844 1103 38-39 0.5375529787823226 1103 40-41 0.6310182141628076 1103 42-43 0.5409760555115497 1103 44-45 0.5560833882347751 1103 46-47 0.5742270473784714 1103 48-49 0.666481891718945 1103 50-51 0.7574756641976492 1103 52-53 0.6734752594692992 1103 54-55 0.718135202384218 1103 56-57 0.638036614008513 1103 58-59 0.6518819593812708 1103 60-61 0.7753994226672916 1103 62-63 0.6365753707428254 1103 64-65 0.7264164760161833 1103 66-67 0.5670251107483111 1103 68-69 0.7323021839909742 1103 70-71 0.8517854914068366 1103 72-73 0.6854905825623625 1103 74-75 0.7796853529347452 1103 76-77 0.5624142342623806 1103 78-79 0.9988589040222742 1103 80-81 0.9926890091090605 1103 82-83 1.0391791749924373 1103 84-85 1.193149101054118 1103 86-87 1.1422175977024942 1103 88-89 1.3774876782385874 1103 90-91 1.3528368947385445 1103 92-93 1.548018260794482 1103 94-95 1.5635186423608722 1103 96-97 1.9216378281470732 1103 98-99 2.1538627411714444 1103 100-101 2.094235616869426 1104 1 0.18529768923863799 1104 2 0.1371699269628408 1104 3 0.2195321460325701 1104 4 0.0982080813110926 1104 5 0.06812291021077499 1104 6 0.09622920113345401 1104 7 0.057892656648917296 1104 8 0.03574300024833832 1104 9 0.09924002453114866 1104 10-11 0.10405566207957406 1104 12-13 0.015985112530870538 1104 14-15 0.13682727234390057 1104 16-17 0.16609560726222838 1104 18-19 0.21133857890651342 1104 20-21 0.23050436773195315 1104 22-23 0.21294106888645814 1104 24-25 0.13877227426993244 1104 26-27 0.11677847115441864 1104 28-29 0.07984348504557204 1104 30-31 0.21049432405118296 1104 32-33 0.2962553762000084 1104 34-35 0.322843778255681 1104 36-37 0.341155948159809 1104 38-39 0.35893521037854015 1104 40-41 0.3163260925776683 1104 42-43 0.2917382769498218 1104 44-45 0.3440253278024912 1104 46-47 0.3281138387927527 1104 48-49 0.34061602667954816 1104 50-51 0.45943257047681385 1104 52-53 0.4830673903116818 1104 54-55 0.46396368817435274 1104 56-57 0.5033712383520594 1104 58-59 0.4989739338512891 1104 60-61 0.5797169261137398 1104 62-63 0.5767202841375649 1104 64-65 0.6266924419595341 1104 66-67 0.603340027170411 1104 68-69 0.7718631404394003 1104 70-71 0.8180323295576883 1104 72-73 0.8580074520127106 1104 74-75 0.8369243924831942 1104 76-77 0.8783256849725589 1104 78-79 0.8881103682930664 1104 80-81 1.0344154100177754 1104 82-83 0.9737903832629087 1104 84-85 1.0689615492948654 1104 86-87 1.197291818280096 1104 88-89 1.1934108881503427 1104 90-91 1.196135525917441 1104 92-93 1.4902628171598344 1104 94-95 1.6055486558464231 1104 96-97 1.9837080230746018 1104 98-99 2.272917401443106 1104 100-101 2.4611417208958315 1105 1 0.07933650127010594 1105 2 0.08968858589167183 1105 3 0.15042742798262054 1105 4 0.121299908235045 1105 5 0.06732656482492416 1105 6 0.1332458523232276 1105 7 0.07284971967605003 1105 8 0.09969929520810439 1105 9 0.07085199908458151 1105 10-11 0.10822840243813658 1105 12-13 0.10874366024499693 1105 14-15 0.13716213877132333 1105 16-17 0.11195661808267232 1105 18-19 0.1659326616862984 1105 20-21 0.17253042364215077 1105 22-23 0.1546912773227902 1105 24-25 0.20012899869064427 1105 26-27 0.30157849085780697 1105 28-29 0.13040822436788346 1105 30-31 0.15489618930553206 1105 32-33 0.21045924061878907 1105 34-35 0.27802595252487805 1105 36-37 0.3049757788930805 1105 38-39 0.2872799066088092 1105 40-41 0.3235236683308145 1105 42-43 0.24697408822782307 1105 44-45 0.2585079587160166 1105 46-47 0.2167559285198024 1105 48-49 0.3489666156832314 1105 50-51 0.3093477364322652 1105 52-53 0.417332590656855 1105 54-55 0.4148458710181657 1105 56-57 0.573895695186124 1105 58-59 0.623080120533622 1105 60-61 0.5631469216074976 1105 62-63 0.7733302062544283 1105 64-65 0.7898361544797581 1105 66-67 0.7136015714419202 1105 68-69 0.710871111190805 1105 70-71 0.8806734231688651 1105 72-73 0.8610888434369848 1105 74-75 0.8221114561123777 1105 76-77 0.9285416925182268 1105 78-79 0.9740486237037409 1105 80-81 1.0125934483992474 1105 82-83 1.0865637450080463 1105 84-85 1.1771884447884418 1105 86-87 1.3563166098893689 1105 88-89 1.3627655473637752 1105 90-91 1.4730046570891169 1105 92-93 1.6277572755811178 1105 94-95 1.65475021241431 1105 96-97 1.835176290580975 1105 98-99 2.086552148008238 1105 100-101 2.3587681008173433 1106 1 0.021241133916753085 1106 2 0.05287203120375494 1106 3 0.11125125104800304 1106 4 0.06190521688638739 1106 5 0.07332126636156744 1106 6 0.0075416436533970455 1106 7 0.05952768574152145 1106 8 -0.02396624828692495 1106 9 0.0529085039155035 1106 10-11 0.059734852392885784 1106 12-13 0.023623333403122615 1106 14-15 0.047424091038131166 1106 16-17 0.08612554053252808 1106 18-19 0.02073200295841815 1106 20-21 -0.004015914401527709 1106 22-23 0.05788424804232051 1106 24-25 0.11139968590569538 1106 26-27 0.11376401259472857 1106 28-29 0.0064105027712528795 1106 30-31 0.08114704023361696 1106 32-33 0.17305589732188764 1106 34-35 0.007796751415881431 1106 36-37 0.1679269310790943 1106 38-39 0.16701691773258887 1106 40-41 0.22132911989889692 1106 42-43 0.23875308096408077 1106 44-45 0.30084837697187794 1106 46-47 0.011204297356115944 1106 48-49 0.31331758241946517 1106 50-51 0.3111642074077352 1106 52-53 0.21275140045113972 1106 54-55 0.3131051508987852 1106 56-57 0.4561895871343751 1106 58-59 0.4284615126466278 1106 60-61 0.30977909874730614 1106 62-63 0.2509739966256461 1106 64-65 0.37714948018279415 1106 66-67 0.3364274078806275 1106 68-69 0.0177817693522897 1106 70-71 0.2480613348782441 1106 72-73 0.4851264620281306 1106 74-75 0.5977489263721978 1106 76-77 0.5217967315620697 1106 78-79 0.7434985866387223 1106 80-81 0.8756105909266303 1106 82-83 0.7977058534715873 1106 84-85 0.9039070345436784 1106 86-87 1.1312240007719794 1106 88-89 1.2386675489461219 1106 90-91 1.1006315802934594 1106 92-93 1.1911288423467816 1106 94-95 1.4695665312824922 1106 96-97 1.5152411338837481 1106 98-99 1.8043158217234918 1106 100-101 2.260960173498269 1107 1 0.09484814609287895 1107 2 0.10964230254234053 1107 3 0.10683094560033624 1107 4 0.07159711484293041 1107 5 0.038083950460247706 1107 6 0.05170229084097855 1107 7 -0.005891578371603146 1107 8 0.05087947556623362 1107 9 0.025549519931324483 1107 10-11 0.036502725887224585 1107 12-13 -0.00982476138397459 1107 14-15 0.1376154945047503 1107 16-17 0.11247460278172383 1107 18-19 0.08201446220423492 1107 20-21 0.002506169586901308 1107 22-23 0.04471547593228564 1107 24-25 0.10098712580906266 1107 26-27 0.11826258828571667 1107 28-29 0.096856088198642 1107 30-31 0.08869161107970314 1107 32-33 0.05991403551625041 1107 34-35 0.07096921453069882 1107 36-37 0.16451052657848209 1107 38-39 0.2209817636606246 1107 40-41 0.20916765178996144 1107 42-43 0.17419191002029777 1107 44-45 0.11092026517998477 1107 46-47 0.2039360021448431 1107 48-49 0.2045787490178128 1107 50-51 0.20491850376348708 1107 52-53 0.21617146977920498 1107 54-55 0.2863958884662523 1107 56-57 0.2641039390698552 1107 58-59 0.34650366758610573 1107 60-61 0.3022915945000335 1107 62-63 0.30597441808080816 1107 64-65 0.2266331845275502 1107 66-67 0.5089771696930185 1107 68-69 0.5328326921296664 1107 70-71 0.652281069555201 1107 72-73 0.49060511728776746 1107 74-75 0.43722032213220174 1107 76-77 0.4732450975463962 1107 78-79 0.689737400407374 1107 80-81 0.6417388420866779 1107 82-83 0.8269399202416032 1107 84-85 0.9675530457193062 1107 86-87 1.1936548598755934 1107 88-89 1.1947118703221662 1107 90-91 1.1915138037338018 1107 92-93 1.3807349728701332 1107 94-95 1.4691474320866078 1107 96-97 1.5645681406778138 1107 98-99 1.76277159955956 1107 100-101 2.298958151560612 1108 1 0.06360221656352394 1108 2 0.06410121238723576 1108 3 0.18025392897288128 1108 4 0.07226168147902712 1108 5 -0.021754460162185296 1108 6 0.08232884426548992 1108 7 0.022333563513377896 1108 8 0.006088847284935639 1108 9 0.014072786409251137 1108 10-11 0.039865349995046984 1108 12-13 -0.03145208570578717 1108 14-15 0.06648842271825828 1108 16-17 0.07180461992754061 1108 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-2.6265965894649064 2316 96-97 -2.7353933191428403 2316 98-99 -3.1572442135341277 2316 100-101 -3.773791278688684 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 5.0 4 18.0 5 42.0 6 73.0 7 176.0 8 453.0 9 867.0 10 1489.0 11 2653.0 12 3858.0 13 5673.0 14 7825.0 15 10513.0 16 13333.0 17 18259.0 18 18518.0 19 14517.0 20 13361.0 21 13833.0 22 14871.0 23 16079.0 24 18550.0 25 21680.0 26 25834.0 27 30753.0 28 37685.0 29 45240.0 30 55400.0 31 68760.0 32 86327.0 33 112475.0 34 154925.0 35 226967.0 36 352835.0 37 504254.0 38 340793.0 39 24389.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.847586583218636 18.938401482800444 14.80586846110852 23.408143472872396 2 15.705732815626991 19.847648691439332 40.14804739955962 24.298571093374058 3 19.880317944399057 23.263768451499075 29.60542391334606 27.250489690755813 4 13.055403981381044 16.65384606887776 36.64651159708123 33.64423835265996 5 13.747355054326047 37.26495937973179 34.41405147133871 14.573634094603449 6 32.85391113781768 36.14197094482344 16.77600650383514 14.228111413523736 7 28.929807779400296 32.38739266155169 20.248046534130697 18.43475302491732 8 26.232412724165417 35.323096880462195 19.294750459951114 19.149739935421273 9 25.78782610232472 17.00727040562722 20.157646960060266 37.0472565319878 10-11 25.174933779881897 26.369016716851835 26.97731394853056 21.478735554735707 12-13 25.957601450988278 23.53119470150688 27.477980015773536 23.033223831731313 14-15 22.633539302385692 25.211274762126735 25.793150221911954 26.362035713575622 16-17 22.318382863488882 28.328718727141904 26.69169518900518 22.661203220364033 18-19 22.27191891432144 27.010606264787683 28.69837426572438 22.019100555166496 20-21 22.644010807300006 26.09105790918952 29.181521549429256 22.083409734081215 22-23 22.52129095540332 25.996394621092794 28.996105218741786 22.486209204762105 24-25 22.547438502045292 26.236100715520983 28.911770969660388 22.304689812773333 26-27 22.032026077645163 26.529920482804126 28.978345672868915 22.459707766681795 28-29 22.203309139069336 26.99677790284223 28.729685157918105 22.07022780017033 30-31 22.26492815851674 26.39539094010179 28.70944246219483 22.63023843918664 32-33 22.001778007147376 26.353013068175795 28.847900855776405 22.797308068900428 34-35 22.75247942156331 26.516233179417632 28.5159220779106 22.215365321108457 36-37 22.711660621871555 26.13618287095953 28.626987535703176 22.52516897146574 38-39 22.266142539585736 26.52953628453118 28.57718942376956 22.62713175211352 40-41 22.769682701796608 26.364185170241132 28.464804723079457 22.401327404882796 42-43 22.351262149694712 26.30143064181234 28.39600939605116 22.95129781244179 44-45 22.448215324914397 26.48485046660006 28.604269504342106 22.462664704143435 46-47 22.86210793934697 26.82159688304435 27.85383658958817 22.462458588020507 48-49 22.579548406822678 26.65949711833643 27.995701805701117 22.765252669139777 50-51 22.6614191319255 26.965251835274834 27.99916934897726 22.37415968382241 52-53 22.90593098085305 26.82284820515313 27.753862195878998 22.51735861811482 54-55 22.532447305575744 26.853555782855455 27.913629967061155 22.700366944507653 56-57 22.619356378008074 26.928734118769842 27.82283274090537 22.62907676231672 58-59 22.727782846614545 26.676114585657572 27.728147361026117 22.86795520670176 60-61 22.413372597794798 26.966113414793096 27.94197372403387 22.67854026337823 62-63 22.388386791765065 26.97400018115262 28.05901598782301 22.578597039259307 64-65 22.060345913130693 27.13113903021493 27.89140563384638 22.917109422808 66-67 22.16681829046997 27.00274866929678 27.75060233457946 23.07983070565379 68-69 22.44400216588362 26.78531308218892 27.93446412606132 22.836220625866137 70-71 22.428991488036196 26.907879476071283 27.51310594997979 23.150023085912732 72-73 22.451392555511127 26.83049196190493 27.611458565757296 23.106656916826648 74-75 22.333400345073642 27.40368535115993 27.287703492931737 22.975210810834692 76-77 22.556969184172562 26.898998579498386 27.512708297894434 23.03132393843462 78-79 22.537705149815423 27.065305518306353 27.560713741309645 22.836275590568576 80-81 22.65875897452438 27.172669418276072 27.271662848832545 22.896908758366997 82-83 22.586689112165637 27.151211084895237 27.38121117326335 22.880888629675777 84-85 22.48469034159886 27.278376967433875 27.324550618804256 22.91238207216301 86-87 22.599979896322463 27.347982507591347 27.122578307690098 22.929459288396092 88-89 22.46399479694108 27.44224763662006 26.919069811830948 23.174687754607906 90-91 22.514001742223016 27.259206597029294 27.03677822623316 23.19001343451453 92-93 22.56281839992577 27.03855483437681 27.266654000627412 23.131972765070007 94-95 22.35937300828641 27.01219268377117 27.271351556267696 23.357082751674724 96-97 22.41701584670203 26.81510472529121 27.282192624548856 23.485686803457902 98-99 22.233771340709062 27.077714644233147 27.005849916934714 23.682664098123077 100-101 22.584711585046726 26.678209200284464 27.04469973911105 23.69237947555776 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 498.0 1 338.5 2 280.5 3 378.0 4 295.0 5 288.5 6 586.0 7 1344.0 8 2199.0 9 2594.5 10 2290.0 11 1823.5 12 1759.5 13 1897.5 14 2051.0 15 2135.0 16 2264.5 17 2404.0 18 2598.5 19 2866.0 20 3276.5 21 3912.0 22 4744.5 23 5981.5 24 7804.5 25 10144.5 26 12875.0 27 16040.0 28 19148.5 29 22113.5 30 25224.5 31 29140.5 32 35013.0 33 38348.0 34 39561.5 35 42893.5 36 47765.5 37 52480.5 38 56011.5 39 58809.5 40 62033.0 41 67382.5 42 71058.0 43 73375.5 44 77151.0 45 80800.0 46 83004.5 47 87500.0 48 91497.0 49 89762.5 50 86554.5 51 83101.0 52 81048.0 53 81712.0 54 83421.5 55 79114.0 56 71349.5 57 65142.0 58 59043.0 59 51350.0 60 40893.0 61 33060.5 62 27453.0 63 20811.5 64 14876.0 65 11048.5 66 8076.0 67 5878.5 68 4268.5 69 2961.0 70 2079.5 71 1419.5 72 975.0 73 739.0 74 517.0 75 356.0 76 242.0 77 144.0 78 68.5 79 27.5 80 19.5 81 14.0 82 12.5 83 7.5 84 3.5 85 2.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.009985485698884586 3 0.005390394934796104 4 0.0 5 2.6510139023587395E-4 6 2.2091782519656163E-4 7 0.0 8 4.418356503931233E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.2091782519656165E-5 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0017231590365331807 26-27 0.022268516779813414 28-29 3.3137673779484245E-4 30-31 1.5464247763759313E-4 32-33 0.003402134508027049 34-35 0.015817716284073816 36-37 0.007997225272115532 38-39 0.020987193393673356 40-41 0.02315218808059966 42-43 0.01853500553399152 44-45 0.009366915788334212 46-47 0.0022533618170049287 48-49 3.976520853538109E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 3.5346852031449864E-4 68-69 2.430096077162178E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.5464247763759313E-4 82-83 0.0011045891259828082 84-85 0.003954429071018454 86-87 7.732123881879657E-4 88-89 4.418356503931233E-5 90-91 0.003733511245821892 92-93 6.627534755896849E-4 94-95 6.627534755896849E-5 96-97 2.8719317275553014E-4 98-99 2.2091782519656163E-4 100-101 0.0027172892499177084 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2263285.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.64206054943874 #Duplication Level Percentage of deduplicated Percentage of total 1 72.9772672467661 37.686964538900675 2 14.654676081058707 15.13595339020891 3 5.2562870666477535 8.14336484883165 4 2.3808483685885085 4.918076624387209 5 1.31826084208835 3.403885311354033 6 0.7740431385960468 2.398390957875279 7 0.5300445680453938 1.9160815573830932 8 0.34188036241125896 1.4124325101045037 9 0.2583308445860931 1.2006663406112403 >10 1.3682866573291865 12.768553871626903 >50 0.09067041937456656 3.158952031313312 >100 0.04339446848922761 4.140672127997772 >500 0.0037776740689458247 1.3754067926077111 >1k 0.002060549492152192 1.7277159500835957 >5k 1.7171245767934933E-4 0.6128831467140283 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7432 0.3283722553721692 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6391 0.2823771641662451 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4129 0.18243394004732058 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2513 0.11103329894379188 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2363 0.10440576418789503 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.418356503931233E-5 0.0 5 0.0 0.0 0.0 4.418356503931233E-5 0.0 6 0.0 0.0 0.0 4.418356503931233E-5 0.0 7 0.0 0.0 0.0 4.418356503931233E-5 0.0 8 0.0 0.0 0.0 4.418356503931233E-5 0.0 9 0.0 0.0 0.0 4.418356503931233E-5 0.0 10-11 0.0 0.0 0.0 6.62753475589685E-5 0.0 12-13 0.0 0.0 0.0 8.836713007862466E-5 6.62753475589685E-5 14-15 0.0 0.0 0.0 8.836713007862466E-5 8.836713007862466E-5 16-17 0.0 0.0 0.0 1.7673426015724932E-4 1.1045891259828082E-4 18-19 0.0 0.0 0.0 2.871931727555301E-4 1.7673426015724932E-4 20-21 0.0 0.0 0.0 3.5346852031449864E-4 1.7673426015724932E-4 22-23 0.0 0.0 0.0 4.197438678734671E-4 1.7673426015724932E-4 24-25 0.0 0.0 0.0 5.964781280307163E-4 1.7673426015724932E-4 26-27 0.0 0.0 0.0 0.0013696905162186822 1.7673426015724932E-4 28-29 0.0 0.0 0.0 0.004175346896215015 1.7673426015724932E-4 30-31 0.0 0.0 0.0 0.014028281899981664 1.9882604267690548E-4 32-33 0.0 0.0 0.0 0.035280576683890896 2.2091782519656163E-4 34-35 0.0 0.0 0.0 0.06642999003660609 2.6510139023587395E-4 36-37 0.0 0.0 0.0 0.11529701297008552 2.6510139023587395E-4 38-39 0.0 0.0 0.0 0.18784642676463637 3.0928495527518627E-4 40-41 0.0 0.0 0.0 0.2642398107176074 3.5346852031449864E-4 42-43 0.0 0.0 0.0 0.34160523310144325 3.5346852031449864E-4 44-45 0.0 0.0 0.0 0.44148218187280874 3.9765208535381095E-4 46-47 0.0 0.0 0.0 0.5524050219040024 4.6392743291277946E-4 48-49 0.0 0.0 0.0 0.6644103592786592 4.860192154324356E-4 50-51 2.2091782519656165E-5 0.0 0.0 0.7821374683259068 4.860192154324356E-4 52-53 4.418356503931233E-5 0.0 0.0 0.8950706605663892 4.860192154324356E-4 54-55 4.418356503931233E-5 0.0 0.0 1.017216126117568 6.185699105503725E-4 56-57 4.418356503931233E-5 0.0 0.0 1.1618952098387962 6.406616930700287E-4 58-59 4.418356503931233E-5 0.0 0.0 1.3109484664989164 6.627534755896849E-4 60-61 4.418356503931233E-5 0.0 0.0 1.471732459676974 6.627534755896849E-4 62-63 4.418356503931233E-5 0.0 0.0 1.6250052467983482 7.069370406289973E-4 64-65 4.418356503931233E-5 0.0 0.0 1.7824091972508986 7.511206056683095E-4 66-67 4.418356503931233E-5 0.0 0.0 1.968178996458687 7.511206056683095E-4 68-69 4.418356503931233E-5 0.0 0.0 2.1691700338225193 7.511206056683095E-4 70-71 4.418356503931233E-5 0.0 0.0 2.3765014127694926 7.511206056683095E-4 72-73 4.418356503931233E-5 0.0 0.0 2.5815794298994605 7.732123881879657E-4 74-75 4.418356503931233E-5 0.0 0.0 2.796510382033195 7.953041707076219E-4 76-77 4.418356503931233E-5 0.0 0.0 3.0254033407193526 7.953041707076219E-4 78-79 4.418356503931233E-5 0.0 0.0 3.2795692986079965 7.953041707076219E-4 80-81 4.418356503931233E-5 0.0 0.0 3.547476345223867 7.953041707076219E-4 82-83 4.418356503931233E-5 0.0 0.0 3.820420318254219 7.953041707076219E-4 84-85 4.418356503931233E-5 0.0 0.0 4.1027753906379445 8.394877357469342E-4 86-87 4.418356503931233E-5 0.0 0.0 4.387516375533792 9.057630833059027E-4 88-89 4.418356503931233E-5 0.0 0.0 4.695078171772446 9.720384308648712E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6550 0.0 32.199955 1 TAGATCG 110 2.7363694E-6 30.225325 5 GTATCAA 9265 0.0 29.2755 1 TCAACGC 11050 0.0 24.629635 4 ATCAACG 10990 0.0 24.505875 3 CAACGCA 11180 0.0 24.30076 5 TATCAAC 11255 0.0 24.183699 2 AACGCAG 11600 0.0 23.502796 6 GTAAGGT 1140 0.0 23.331831 4 GTACATG 6275 0.0 23.164413 1 GAGTACT 7650 0.0 22.320457 12-13 TACATGG 6220 0.0 22.223694 2 TAAGGTG 1335 0.0 22.058502 5 TATACCG 110 0.0028792953 21.589518 5 TTACTCG 155 3.9105173E-5 21.45023 4 GTACTTT 7960 0.0 21.391523 14-15 CGCAGAG 12805 0.0 21.179815 8 ACGCAGA 12930 0.0 20.97506 7 ACATGGG 6140 0.0 20.655119 3 AGAGTAC 10385 0.0 20.512642 10-11 >>END_MODULE