FastQCFastQC Report
Thu 26 May 2016
SRR938093_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938093_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2320364
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76960.33167209972228495No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64200.27668072767893315No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40680.1753173209030997No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT23750.10235463056658353No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA23720.10222534050692046No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA94700.031.310091
GGTATCA65600.030.7150541
GTACATG66050.025.879321
ATCAACG112600.025.5763323
TATCAAC113600.025.5603542
TACATGG65650.025.4807342
TCAACGC113650.025.3818474
CAACGCA114550.025.0579685
AACGCAG117850.024.4369536
ACATGGG66850.024.3124473
GAGTACT73950.022.08602512-13
CATGGGG42400.021.7440224
ACGCAGA131650.021.6019887
CGCAGAG131850.021.4610168
GTACTTT77950.020.78358314-15
AGAGTAC103950.020.65379110-11
AGTACTT78900.020.21829612-13
GTCCTAC5950.020.0616051
TAAGGTG13550.019.9912325
GCAGAGT140550.019.6229219
Produced by FastQC (version 0.11.5)