##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938093_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2320364 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.663753617966837 33.0 31.0 34.0 30.0 34.0 2 31.869109760365184 33.0 31.0 34.0 30.0 34.0 3 31.93342208377651 34.0 31.0 34.0 30.0 34.0 4 35.46348848715115 37.0 35.0 37.0 33.0 37.0 5 35.47798319573998 37.0 35.0 37.0 33.0 37.0 6 35.49358807497445 37.0 35.0 37.0 33.0 37.0 7 35.42419939285388 37.0 35.0 37.0 33.0 37.0 8 35.397238536712344 37.0 35.0 37.0 33.0 37.0 9 37.02929066301667 39.0 37.0 39.0 33.0 39.0 10-11 36.93385757579414 39.0 37.0 39.0 33.0 39.0 12-13 36.82762014925244 39.0 37.0 39.0 33.0 39.0 14-15 38.03780290506145 40.0 38.0 41.0 33.0 41.0 16-17 37.94517627406734 40.0 38.0 41.0 33.0 41.0 18-19 37.87052742586939 40.0 38.0 41.0 32.5 41.0 20-21 37.753791000032756 40.0 38.0 41.0 32.0 41.0 22-23 37.592938220037894 40.0 37.5 41.0 32.0 41.0 24-25 37.537567381669426 40.0 37.5 41.0 32.0 41.0 26-27 37.54546398754678 40.0 37.5 41.0 32.0 41.0 28-29 37.4507320834145 40.0 37.0 41.0 32.0 41.0 30-31 37.271231367147564 40.0 37.0 41.0 31.0 41.0 32-33 37.04999689703857 40.0 36.5 41.0 31.0 41.0 34-35 36.826226186925844 40.0 36.0 41.0 30.0 41.0 36-37 36.59726728220228 39.5 36.0 41.0 30.0 41.0 38-39 36.338523610950695 39.0 35.5 41.0 30.0 41.0 40-41 36.11485094579989 39.0 35.0 41.0 29.0 41.0 42-43 35.82439263839639 39.0 35.0 40.0 27.5 41.0 44-45 35.60788070320002 39.0 35.0 40.0 27.0 41.0 46-47 35.47776857424094 39.0 35.0 40.5 26.5 41.0 48-49 35.229573678957266 39.0 35.0 40.0 25.5 41.0 50-51 34.608097695016816 38.5 34.0 39.5 24.0 40.5 52-53 34.71061027493962 38.0 34.5 40.0 24.0 41.0 54-55 34.84774651735676 39.0 34.5 40.0 24.0 41.0 56-57 34.625944463885844 38.0 34.0 40.0 23.5 41.0 58-59 34.355481295176105 38.0 34.0 40.0 23.0 41.0 60-61 33.96343267694206 38.0 34.0 40.0 22.0 41.0 62-63 33.52932751068367 37.0 33.0 40.0 20.5 41.0 64-65 33.08040376423699 36.5 33.0 39.5 19.0 41.0 66-67 32.62662733088429 36.0 32.0 39.0 17.5 41.0 68-69 32.117299053079606 35.5 31.5 39.0 12.5 40.0 70-71 31.615449774259552 35.0 31.0 38.0 8.0 40.0 72-73 31.099618206453815 35.0 31.0 37.5 7.0 39.5 74-75 30.550678471136425 35.0 30.0 37.0 3.5 39.0 76-77 29.985510678496993 34.0 29.5 36.0 2.0 39.0 78-79 29.392846337902157 34.0 29.0 36.0 2.0 38.0 80-81 28.834946801450116 34.0 28.5 35.0 2.0 37.0 82-83 28.27241932731244 34.0 27.0 35.0 2.0 37.0 84-85 27.76098512991927 33.5 26.0 35.0 2.0 36.0 86-87 27.23913769563741 33.0 26.0 35.0 2.0 36.0 88-89 26.651984128352275 33.0 25.0 35.0 2.0 35.0 90-91 26.00462578285131 32.0 23.5 35.0 2.0 35.0 92-93 25.33712167573708 32.0 20.0 34.0 2.0 35.0 94-95 24.693195550353305 31.5 17.5 34.0 2.0 35.0 96-97 23.79499681946453 31.0 4.5 34.0 2.0 35.0 98-99 22.658871194347093 31.0 2.0 34.0 2.0 35.0 100-101 19.802274556922967 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5146473526618927 1101 2 0.515793778050174 1101 3 0.4821523484131518 1101 4 0.36271436013237945 1101 5 0.39424829647812487 1101 6 0.41280966551603626 1101 7 0.3800200081102574 1101 8 0.5057482563665161 1101 9 0.4630320037985456 1101 10-11 0.5166678120529511 1101 12-13 0.42386418926520975 1101 14-15 0.5633526055973306 1101 16-17 0.7659902378420114 1101 18-19 0.7414085215724882 1101 20-21 0.7757057737638533 1101 22-23 0.6630627167618641 1101 24-25 0.680143530992126 1101 26-27 0.7581658196808121 1101 28-29 0.7444324880052022 1101 30-31 0.782439790110125 1101 32-33 0.7708743804277205 1101 34-35 0.7872218227597898 1101 36-37 0.7301175088910554 1101 38-39 0.8612022326031692 1101 40-41 1.0136569832839726 1101 42-43 0.9075348692567999 1101 44-45 0.8498260667060649 1101 46-47 0.7776862142256107 1101 48-49 0.8722794326130341 1101 50-51 0.9094890230233759 1101 52-53 0.9108622019254895 1101 54-55 0.9525157555884007 1101 56-57 0.9335957066410074 1101 58-59 0.8075457377365964 1101 60-61 0.8088163373136368 1101 62-63 0.9282806916157043 1101 64-65 0.9959185418366303 1101 66-67 0.8987794255660475 1101 68-69 1.1297238985563283 1101 70-71 1.2471859844199322 1101 72-73 1.3455339461763423 1101 74-75 1.1950986583946666 1101 76-77 1.1824357537864394 1101 78-79 1.2971777188334848 1101 80-81 1.301318778878688 1101 82-83 1.1995426355407304 1101 84-85 1.3608616137704033 1101 86-87 1.1820771835858999 1101 88-89 1.3600602104091415 1101 90-91 1.5670389491469336 1101 92-93 1.641901998501421 1101 94-95 1.8313769567141804 1101 96-97 1.8418320930781213 1101 98-99 1.7970604888035453 1101 100-101 1.6655297757740328 1102 1 0.5962465224000688 1102 2 0.4043494429845822 1102 3 0.49817762255980824 1102 4 0.3796998788806789 1102 5 0.30032492781287345 1102 6 0.4807911677710237 1102 7 0.28922792713937895 1102 8 0.40617788633530694 1102 9 0.5011759718090474 1102 10-11 0.4849676576507065 1102 12-13 0.4038459509770149 1102 14-15 0.52277305028597 1102 16-17 0.5414208216961711 1102 18-19 0.5634898231299914 1102 20-21 0.5123698204253841 1102 22-23 0.46097383499531475 1102 24-25 0.520681242532099 1102 26-27 0.437476554833907 1102 28-29 0.4815416811708033 1102 30-31 0.4638039224941508 1102 32-33 0.6318506757404521 1102 34-35 0.6085597296554894 1102 36-37 0.6342793743347102 1102 38-39 0.546540251421419 1102 40-41 0.8011173334489285 1102 42-43 0.8057419463231028 1102 44-45 0.7145428645001672 1102 46-47 0.834757811589327 1102 48-49 0.7842218230243176 1102 50-51 1.0202987063588722 1102 52-53 0.8721579852434402 1102 54-55 0.8985315671922223 1102 56-57 0.7505063135919059 1102 58-59 0.916791684995637 1102 60-61 1.0226119362137354 1102 62-63 0.9746382485499439 1102 64-65 0.9597898156424236 1102 66-67 1.0648476881465285 1102 68-69 0.9347878851866724 1102 70-71 1.0677296227676472 1102 72-73 1.1228907425459802 1102 74-75 1.1282363816356415 1102 76-77 1.1742437862643307 1102 78-79 1.043517342722847 1102 80-81 1.186444272037427 1102 82-83 1.402762189427623 1102 84-85 1.3072691542978134 1102 86-87 1.4355982924331236 1102 88-89 1.5092707748803456 1102 90-91 1.5661651901517963 1102 92-93 1.4562345712701585 1102 94-95 1.5707099746294197 1102 96-97 1.5517949805781548 1102 98-99 1.6073320536079692 1102 100-101 1.4556444709504248 1103 1 0.5037584487590046 1103 2 0.4309233864099724 1103 3 0.3902879348474997 1103 4 0.2719184429396009 1103 5 0.33451009060114245 1103 6 0.17173236092951072 1103 7 0.3371200038562918 1103 8 0.3361651200919411 1103 9 0.372837897794696 1103 10-11 0.4138350454981392 1103 12-13 0.28886017302006195 1103 14-15 0.5213340482926512 1103 16-17 0.4900422913819398 1103 18-19 0.5318205808177296 1103 20-21 0.5375924831203278 1103 22-23 0.6151411549054373 1103 24-25 0.685231400430375 1103 26-27 0.5651464541224271 1103 28-29 0.723969656414738 1103 30-31 0.7167416515959673 1103 32-33 0.6626203085056517 1103 34-35 0.6855939297495581 1103 36-37 0.7931519134733236 1103 38-39 0.8529207601300897 1103 40-41 0.9388397161823931 1103 42-43 0.8461889141536645 1103 44-45 0.917989003644756 1103 46-47 0.8929562034906056 1103 48-49 0.8932108261374907 1103 50-51 0.805999393944262 1103 52-53 0.8147957449543668 1103 54-55 0.9029176303366029 1103 56-57 0.7842845990331639 1103 58-59 1.0452380107812118 1103 60-61 0.8980608077823433 1103 62-63 0.9815178352521414 1103 64-65 0.9483785858276477 1103 66-67 0.9648529140941093 1103 68-69 1.0424237411595527 1103 70-71 1.2707072930402212 1103 72-73 1.3660092894339293 1103 74-75 1.1327661583383666 1103 76-77 1.1253587795079234 1103 78-79 1.2380588404794217 1103 80-81 1.3488405929703333 1103 82-83 1.2279747735093842 1103 84-85 1.4729223604166535 1103 86-87 1.336176506204243 1103 88-89 1.4553277974340446 1103 90-91 1.550050607518365 1103 92-93 1.6850209569301562 1103 94-95 1.731814739947044 1103 96-97 1.727422280928291 1103 98-99 2.0617785884314443 1103 100-101 1.8708049448692137 1104 1 0.37301564526798003 1104 2 0.295208643435771 1104 3 0.26300557278504044 1104 4 0.2524539044621932 1104 5 0.18136510770774095 1104 6 0.260419953842181 1104 7 0.33739029527311715 1104 8 0.35761061013739237 1104 9 0.3247011303377789 1104 10-11 0.4596120977568461 1104 12-13 0.3153789618299925 1104 14-15 0.3298343577051881 1104 16-17 0.38667182854345583 1104 18-19 0.5970212721947306 1104 20-21 0.4821987467682689 1104 22-23 0.35532175224945917 1104 24-25 0.553683057387893 1104 26-27 0.5140222552163891 1104 28-29 0.4956303126538444 1104 30-31 0.4576851108993196 1104 32-33 0.5025295474930473 1104 34-35 0.6556555988701831 1104 36-37 0.5145205838529421 1104 38-39 0.5082547478531652 1104 40-41 0.5335110631583362 1104 42-43 0.582491185312854 1104 44-45 0.5429721104312009 1104 46-47 0.5653149940076432 1104 48-49 0.7023779729270743 1104 50-51 0.6693030663510626 1104 52-53 0.6149401374744059 1104 54-55 0.6273733132136385 1104 56-57 0.7349137378704853 1104 58-59 0.7186904145965869 1104 60-61 0.6108583950758018 1104 62-63 0.5434021220740206 1104 64-65 0.6226098556994089 1104 66-67 0.6645129159434617 1104 68-69 0.6248217239320368 1104 70-71 0.7996533699025612 1104 72-73 0.6787933621565436 1104 74-75 0.6532087249687528 1104 76-77 0.7445875242888107 1104 78-79 0.8309821844110239 1104 80-81 0.8708871257716169 1104 82-83 0.9043203624245955 1104 84-85 0.9753656525661114 1104 86-87 1.0583631637663586 1104 88-89 1.1095379418976954 1104 90-91 0.9981279819928979 1104 92-93 0.994991865038191 1104 94-95 1.2452759637981288 1104 96-97 1.371574739513246 1104 98-99 1.4585717230294577 1104 100-101 1.1896759091694094 1105 1 0.37881409906276886 1105 2 0.31363994314587984 1105 3 0.3257297773310768 1105 4 0.2382706520949398 1105 5 0.16336019741167718 1105 6 0.26177245958940887 1105 7 0.24694672498225145 1105 8 0.3358603276590131 1105 9 0.40757472227610947 1105 10-11 0.5050513157063321 1105 12-13 0.33889008149100874 1105 14-15 0.46838235618094615 1105 16-17 0.5601023138018846 1105 18-19 0.42480361974669023 1105 20-21 0.5250912369340384 1105 22-23 0.44566895189921496 1105 24-25 0.46797546839338366 1105 26-27 0.4223776450807861 1105 28-29 0.6015707611255934 1105 30-31 0.6119927495716979 1105 32-33 0.48341738828212755 1105 34-35 0.5714742776010553 1105 36-37 0.5037423660501332 1105 38-39 0.6320029429766336 1105 40-41 0.6818835233404954 1105 42-43 0.6124408956849905 1105 44-45 0.635822367038763 1105 46-47 0.591881781625915 1105 48-49 0.5251785295550491 1105 50-51 0.533170783181319 1105 52-53 0.5449558884541474 1105 54-55 0.47055377285921907 1105 56-57 0.5643915084987796 1105 58-59 0.5213563250393278 1105 60-61 0.5761758359138085 1105 62-63 0.5897903052276163 1105 64-65 0.301479477830668 1105 66-67 0.3253066857197169 1105 68-69 0.5017158769059549 1105 70-71 0.5476620170946269 1105 72-73 0.6344726332923081 1105 74-75 0.5367788020873796 1105 76-77 0.5816509358021378 1105 78-79 0.6718534339715525 1105 80-81 0.7852801455152516 1105 82-83 0.8317153110551736 1105 84-85 0.7462635247465741 1105 86-87 0.8155771419685323 1105 88-89 0.9454985268582696 1105 90-91 0.8555873275864911 1105 92-93 0.8854501396830905 1105 94-95 0.8891416684536217 1105 96-97 1.2374452335869144 1105 98-99 1.0560630644129674 1105 100-101 0.9708835972985348 1106 1 0.1418571975046774 1106 2 0.19712723232752438 1106 3 0.0317975405396993 1106 4 0.12043907760018868 1106 5 0.13724924309762088 1106 6 0.2776907798755772 1106 7 0.290006262357835 1106 8 0.1856152644674438 1106 9 0.24195564858597152 1106 10-11 -0.36484891892337146 1106 12-13 -0.0689128946350479 1106 14-15 0.056685566795238174 1106 16-17 0.22672936858017323 1106 18-19 0.37716643352059265 1106 20-21 0.20378188011085996 1106 22-23 0.10525729242281301 1106 24-25 0.14989182789107502 1106 26-27 0.21836651696956721 1106 28-29 0.010859359904557664 1106 30-31 0.12992066583782957 1106 32-33 0.155305396985419 1106 34-35 0.059359229115401035 1106 36-37 -0.10772389629232748 1106 38-39 0.312080355924202 1106 40-41 0.3986677170652584 1106 42-43 0.2067835045663955 1106 44-45 0.4007737328354537 1106 46-47 0.5908410853620936 1106 48-49 0.580629337602204 1106 50-51 0.5722441974502317 1106 52-53 0.38930044256221663 1106 54-55 0.005665998290474761 1106 56-57 0.11208581365320214 1106 58-59 0.3341528394760971 1106 60-61 0.11219276858138016 1106 62-63 0.15305049096493661 1106 64-65 0.22392417503668582 1106 66-67 0.02788948694514204 1106 68-69 -0.25578049613051235 1106 70-71 0.12295961908992936 1106 72-73 0.37554899440276657 1106 74-75 0.3557751075714357 1106 76-77 0.47078256539985475 1106 78-79 0.507504593291273 1106 80-81 0.34790141271371056 1106 82-83 0.45395883645664625 1106 84-85 0.4414498668504052 1106 86-87 0.41047414044842867 1106 88-89 0.33220587351959097 1106 90-91 0.15341390815389033 1106 92-93 0.38396504754654615 1106 94-95 0.09788058154584789 1106 96-97 0.06407602320039274 1106 98-99 0.01804555454359047 1106 100-101 0.0929674372749929 1107 1 0.3012303945975958 1107 2 0.2764127077291718 1107 3 0.23630719528078004 1107 4 0.06321537516283371 1107 5 0.16243967555282524 1107 6 0.09270784023905776 1107 7 0.07775149561043548 1107 8 0.07171245751477784 1107 9 0.22661546685799294 1107 10-11 0.2178130130756486 1107 12-13 0.08252144898300173 1107 14-15 0.23576861141084038 1107 16-17 0.215175102276838 1107 18-19 0.2929272893577419 1107 20-21 0.24667629176828854 1107 22-23 0.3157597976742892 1107 24-25 0.3534120623814019 1107 26-27 0.327582902193285 1107 28-29 0.40259501065865777 1107 30-31 0.3356094407552064 1107 32-33 0.28531425806848887 1107 34-35 0.3350318681722584 1107 36-37 0.3310335919290992 1107 38-39 0.4814505622752776 1107 40-41 0.5102135466475346 1107 42-43 0.43387427054994276 1107 44-45 0.33578647885175883 1107 46-47 0.4432409030885083 1107 48-49 0.4058682180543727 1107 50-51 0.5025937606099546 1107 52-53 0.3982309896557936 1107 54-55 0.41917697764728246 1107 56-57 0.40829605876268005 1107 58-59 0.4879214267850429 1107 60-61 0.3441154518426188 1107 62-63 0.34428317169010825 1107 64-65 0.3373212839188966 1107 66-67 0.4154699262810766 1107 68-69 0.37789884380467953 1107 70-71 0.48672678175496387 1107 72-73 0.6017571884291648 1107 74-75 0.650850942074289 1107 76-77 0.6563838611185453 1107 78-79 0.6503685923374682 1107 80-81 0.4752620527324609 1107 82-83 0.41872921113799677 1107 84-85 0.6044980895313437 1107 86-87 0.7769847530352081 1107 88-89 0.719756001282871 1107 90-91 0.821006845961616 1107 92-93 0.8825439399818897 1107 94-95 1.0166594596337113 1107 96-97 1.0918932060226219 1107 98-99 0.9276852408794376 1107 100-101 1.068739649848034 1108 1 0.2113164132275358 1108 2 0.1363835891601468 1108 3 0.14135688998150187 1108 4 0.15731440801036456 1108 5 0.25867951748059426 1108 6 0.25165808611268403 1108 7 0.20208035716352413 1108 8 0.03742979940540181 1108 9 0.15221158042041338 1108 10-11 0.23027085800440972 1108 12-13 0.06575970127460096 1108 14-15 0.14466537419850312 1108 16-17 0.2254683678649272 1108 18-19 0.23928821104959042 1108 20-21 0.28105328809875374 1108 22-23 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2604.0 5 2826.0 6 3214.0 7 3727.0 8 4637.0 9 5712.0 10 7092.0 11 8480.0 12 10136.0 13 12200.0 14 13880.0 15 16014.0 16 18859.0 17 23341.0 18 21666.0 19 18643.0 20 18534.0 21 19592.0 22 21137.0 23 23295.0 24 25804.0 25 28771.0 26 32480.0 27 37488.0 28 43379.0 29 50910.0 30 61005.0 31 74737.0 32 91620.0 33 118204.0 34 159057.0 35 228074.0 36 332548.0 37 435964.0 38 302268.0 39 31254.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.44698218193835 18.525430768824304 14.450998489715818 23.576588559521525 2 15.465120841603092 19.515193431508287 40.4860225086916 24.53366321819702 3 19.530138946264508 23.28365443078258 29.509587214632347 27.676619408320562 4 12.784171768346642 16.341202771725914 36.851206894469826 34.02341856545761 5 13.768323784786993 37.10161254913192 34.4041392225932 14.725924443487889 6 32.82872537738816 35.817198692402705 16.971627451259558 14.382448478949572 7 28.876991509963695 32.329934041138245 20.31855533963164 18.474519109266414 8 26.216496725724504 35.01916012796128 19.444586978184578 19.319756168129636 9 26.009496476334697 16.89897618809439 20.158754661138268 36.93277267443265 10-11 25.131949549608613 26.233867112166443 27.087379876041283 21.546803462183664 12-13 26.022796571907094 23.55020183588358 27.418314872607073 23.008686719602252 14-15 22.760483818805113 25.13233867316358 25.74049718634786 26.36668032168345 16-17 22.28730441562295 28.29625761336606 26.6639010447841 22.75253692622689 18-19 22.315263787823543 26.916390449144462 28.682342698316564 22.08600306471543 20-21 22.69636665776765 26.113415414155643 29.095224766228373 22.094993161848333 22-23 22.52434113708769 25.984593739387307 28.88631818402342 22.604746939501588 24-25 22.496416535139954 26.152758480723048 28.91283520979068 22.437989774346317 26-27 22.015636862872444 26.468729400851426 28.88334582893723 22.632287907338895 28-29 22.16256987432325 26.991800952731708 28.70099956615603 22.144629606789014 30-31 22.228676967664097 26.416484183537598 28.61536006589878 22.739478782899525 32-33 21.9829754659105 26.39208181264387 28.776681024490962 22.848261696954665 34-35 22.7772801237958 26.528395532413807 28.46421441377487 22.23010993001552 36-37 22.75184003922772 26.09096164623592 28.570877170360042 22.586321144176317 38-39 22.235486989785194 26.58571996753509 28.460342045612546 22.718450997067162 40-41 22.745594969596915 26.43736119416885 28.38140813516966 22.43563570106457 42-43 22.37222454445094 26.345262067762754 28.281799616835855 23.000713770950448 44-45 22.45155055258568 26.519133751877533 28.480087108460765 22.549228587076023 46-47 22.81641761415257 26.886463135291912 27.790613536325843 22.50650571422967 48-49 22.56407526165089 26.6954822489518 27.91676402146145 22.823678467935856 50-51 22.622638394287012 27.029805148534315 27.88941734234516 22.458139114833518 52-53 22.842981391306747 26.85037262218421 27.69381612707434 22.612829859434704 54-55 22.45623526267464 26.90306019079313 27.91209549140224 22.72860905512999 56-57 22.59264152744959 26.988688839521362 27.766906336504384 22.651763296524663 58-59 22.679365613055 26.69276718274995 27.702669645272216 22.92519755892283 60-61 22.419691573914534 26.92162780481896 27.94498716004954 22.71369346121697 62-63 22.37478873002661 26.87925207088757 28.137324081781145 22.608635117304676 64-65 22.11917792157857 27.016896592748925 27.9537570111506 22.910168474521914 66-67 22.175981641316003 26.875480605105533 27.8297942406698 23.118743512908665 68-69 22.47167545891802 26.62510094547683 28.010071810031334 22.893151785573814 70-71 22.460833502558643 26.718218947802292 27.685821247197 23.13512630244206 72-73 22.41597216967822 26.64908948518518 27.80796767557619 23.12697066956041 74-75 22.300115794928303 27.275417762752312 27.506080742605032 22.91838569971435 76-77 22.568893117143464 26.780060810136547 27.590352538867386 23.060693533852607 78-79 22.518155109809967 26.8960063930814 27.688417453732917 22.89742104337571 80-81 22.717417541681755 26.923309022569352 27.418791703305228 22.94048173244366 82-83 22.641078777807593 26.9185797773345 27.470016886885883 22.97032455797202 84-85 22.515867901471378 27.06153405160058 27.507279298773458 22.915318748154583 86-87 22.668986052437457 27.03567801195998 27.261718993282557 23.033616942320005 88-89 22.59234803611206 27.078503133257147 27.133603792201534 23.19554503842926 90-91 22.63299247927721 26.96589727464081 27.151570710597596 23.24953953548439 92-93 22.74905473005729 26.703032516078913 27.419370483952775 23.128542269911026 94-95 22.57526075078893 26.572357926205214 27.48201311661037 23.370368206395483 96-97 22.683902984809173 26.381224730013336 27.48298806442816 23.451884220749335 98-99 22.580029781330538 26.49503745084227 27.179408805237514 23.745523962589683 100-101 23.034085238133887 25.853944089779606 27.350083835975042 23.76188683611146 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 567.0 1 386.5 2 298.0 3 402.0 4 352.5 5 361.5 6 669.0 7 1391.0 8 2287.5 9 2698.5 10 2294.0 11 1807.0 12 1811.5 13 1943.5 14 2131.5 15 2282.0 16 2367.0 17 2533.5 18 2776.0 19 3091.0 20 3542.5 21 4224.5 22 5127.0 23 6428.5 24 8042.0 25 10369.0 26 13366.5 27 16731.0 28 19863.5 29 22809.0 30 26002.5 31 29890.0 32 35669.0 33 38977.0 34 40421.5 35 44157.0 36 49176.5 37 53834.5 38 57145.0 39 60123.0 40 63388.0 41 68294.0 42 72235.0 43 74483.5 44 78056.5 45 81924.5 46 84339.0 47 88341.5 48 92340.0 49 91114.0 50 87392.5 51 84351.5 52 83029.5 53 83491.5 54 84246.5 55 80025.0 56 72765.0 57 66622.5 58 60741.5 59 52997.0 60 42814.5 61 34959.0 62 29031.0 63 22255.5 64 16514.0 65 12731.0 66 9384.5 67 6880.5 68 5108.5 69 3602.5 70 2591.0 71 1834.0 72 1291.5 73 998.5 74 736.5 75 485.0 76 330.0 77 209.5 78 129.5 79 83.5 80 45.5 81 20.5 82 17.0 83 16.0 84 12.0 85 8.5 86 4.5 87 4.0 88 4.5 89 2.0 90 3.5 91 3.5 92 1.0 93 1.5 94 1.0 95 0.0 96 0.5 97 1.0 98 1.5 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4967755059120034 2 0.05046621995514497 3 0.05766336660972158 4 0.06550696356261346 5 0.08020293367764712 6 0.10881913354973616 7 0.07093714606846167 8 0.05279344102907992 9 0.05119886362656893 10-11 0.07625958685792401 12-13 0.10840971502746984 14-15 0.056629046132417156 16-17 0.061024908160960956 18-19 0.0598397492807163 20-21 0.07563468490288593 22-23 0.06470967486135795 24-25 0.08951181797338693 26-27 0.06673521912941245 28-29 0.06714463765167879 30-31 0.07138966127728236 32-33 0.0711741778445106 34-35 0.07356604394827708 36-37 0.06876076339746695 38-39 0.04036004695814967 40-41 0.04426029709131843 42-43 0.04277346140519332 44-45 0.0214406015607896 46-47 0.02898252170780102 48-49 0.02887477999141514 50-51 0.025728721872947523 52-53 0.025340851693958364 54-55 0.02473749808219745 56-57 0.024802143112028975 58-59 0.020966538008691738 60-61 0.02805594294688247 62-63 0.020039959247773194 64-65 0.021031183038523266 66-67 0.016031967398218556 68-69 0.01825144675576763 70-71 0.041846882644274774 72-73 0.037903535824551665 74-75 0.03215012816954581 76-77 0.022431825351539675 78-79 0.015342420413348939 80-81 0.017411061367957784 82-83 0.022259438605322267 84-85 0.01848847853181656 86-87 0.009028755833136526 88-89 0.0419115276741063 90-91 0.053375246297563655 92-93 0.03398173734810573 94-95 0.05359072973033541 96-97 0.044669715613584765 98-99 0.03404638237793726 100-101 0.16962855827792536 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2320364.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.58657738791888 #Duplication Level Percentage of deduplicated Percentage of total 1 74.74609299639665 40.0538729679599 2 13.77184225766518 14.75971781829173 3 4.867928584036201 7.825668953619546 4 2.2538629573973354 4.831072071533441 5 1.1959027496511956 3.204216762130438 6 0.7077344549125998 2.2755040282962367 7 0.487776114405729 1.8296776751806676 8 0.3288209987421614 1.4096313516695687 9 0.23950110364603103 1.1550639982518018 >10 1.2742570167191838 12.284882085267657 >50 0.08054798675492844 2.9009845039430346 >100 0.040243394107658016 3.9418090592958483 >500 0.0035519553656460628 1.368221163390241 >1k 0.001775977682822569 1.5490414379155837 >5k 1.6145251662023357E-4 0.610636123254388 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7696 0.33167209972228495 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6420 0.27668072767893315 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4068 0.1753173209030997 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2375 0.10235463056658353 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2372 0.10222534050692046 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.309668655435096E-5 2 0.0 0.0 0.0 0.0 4.309668655435096E-5 3 0.0 0.0 0.0 0.0 4.309668655435096E-5 4 0.0 0.0 0.0 4.309668655435096E-5 4.309668655435096E-5 5 0.0 0.0 0.0 4.309668655435096E-5 4.309668655435096E-5 6 0.0 0.0 0.0 4.309668655435096E-5 4.309668655435096E-5 7 0.0 0.0 0.0 4.309668655435096E-5 4.309668655435096E-5 8 0.0 0.0 0.0 4.309668655435096E-5 4.309668655435096E-5 9 0.0 0.0 0.0 4.309668655435096E-5 4.309668655435096E-5 10-11 0.0 0.0 0.0 8.619337310870191E-5 4.309668655435096E-5 12-13 0.0 0.0 0.0 1.7238674621740383E-4 2.5858011932610575E-4 14-15 0.0 0.0 0.0 1.7238674621740383E-4 3.016768058804567E-4 16-17 0.0 0.0 0.0 2.801284626032812E-4 3.4477349243480765E-4 18-19 0.0 0.0 0.0 4.3096686554350955E-4 3.4477349243480765E-4 20-21 0.0 0.0 0.0 4.7406355209786053E-4 4.5251520882068504E-4 22-23 0.0 0.0 0.0 6.679986415924398E-4 5.171602386522115E-4 24-25 0.0 0.0 0.0 7.326436714239663E-4 5.171602386522115E-4 26-27 0.0 0.0 0.0 0.001249803910076178 5.38708581929387E-4 28-29 0.0 0.0 0.0 0.004869925580641658 5.602569252065624E-4 30-31 4.309668655435096E-5 0.0 0.0 0.014933001891082605 5.602569252065624E-4 32-33 4.309668655435096E-5 0.0 0.0 0.03740792392917663 5.602569252065624E-4 34-35 4.309668655435096E-5 0.0 0.0 0.06705844427857008 6.464502983152643E-4 36-37 4.309668655435096E-5 0.0 0.0 0.11618866695053018 6.464502983152643E-4 38-39 4.309668655435096E-5 0.0 0.0 0.19003483936141052 6.464502983152643E-4 40-41 4.309668655435096E-5 0.0 0.0 0.26797519699495426 6.464502983152643E-4 42-43 4.309668655435096E-5 0.0 0.0 0.345182910957074 6.464502983152643E-4 44-45 4.309668655435096E-5 0.0 0.0 0.43842259231741226 6.464502983152643E-4 46-47 4.309668655435096E-5 0.0 0.0 0.5484915297772246 6.895469848696153E-4 48-49 4.309668655435096E-5 0.0 0.0 0.6621805889076025 7.326436714239663E-4 50-51 4.309668655435096E-5 0.0 0.0 0.7816015073497089 7.326436714239663E-4 52-53 4.309668655435096E-5 0.0 0.0 0.8956568883157987 8.188370445326681E-4 54-55 4.309668655435096E-5 0.0 0.0 1.0210036011591286 8.188370445326681E-4 56-57 4.309668655435096E-5 0.0 0.0 1.1628563449527747 8.188370445326681E-4 58-59 4.309668655435096E-5 0.0 0.0 1.3086955322526983 8.188370445326681E-4 60-61 4.309668655435096E-5 0.0 0.0 1.464727085922726 8.619337310870191E-4 62-63 4.309668655435096E-5 0.0 0.0 1.6213619932045145 8.619337310870191E-4 64-65 4.309668655435096E-5 0.0 0.0 1.7788157375308358 8.619337310870191E-4 66-67 4.309668655435096E-5 0.0 0.0 1.9600157561486042 8.619337310870191E-4 68-69 4.309668655435096E-5 0.0 0.0 2.163690696804467 8.619337310870191E-4 70-71 4.309668655435096E-5 0.0 0.0 2.3692187949821664 8.834820743641946E-4 72-73 4.309668655435096E-5 0.0 0.0 2.573755669369116 9.91223790750072E-4 74-75 4.309668655435096E-5 0.0 0.0 2.7845846599930013 0.001077417163858774 76-77 4.309668655435096E-5 0.0 0.0 3.00773930297143 0.0011205138504131248 78-79 4.309668655435096E-5 0.0 0.0 3.2612555616274 0.0011205138504131248 80-81 4.309668655435096E-5 0.0 0.0 3.5253520568324626 0.0011205138504131248 82-83 4.309668655435096E-5 0.0 0.0 3.791000032753482 0.0011205138504131248 84-85 4.309668655435096E-5 0.0 0.0 4.065827602910578 0.0011205138504131248 86-87 4.309668655435096E-5 0.0 0.0 4.348347931617626 0.0011636105369674757 88-89 4.309668655435096E-5 0.0 0.0 4.645844358902311 0.0012067072235218267 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 9470 0.0 31.31009 1 GGTATCA 6560 0.0 30.715054 1 GTACATG 6605 0.0 25.87932 1 ATCAACG 11260 0.0 25.576332 3 TATCAAC 11360 0.0 25.560354 2 TACATGG 6565 0.0 25.480734 2 TCAACGC 11365 0.0 25.381847 4 CAACGCA 11455 0.0 25.057968 5 AACGCAG 11785 0.0 24.436953 6 ACATGGG 6685 0.0 24.312447 3 GAGTACT 7395 0.0 22.086025 12-13 CATGGGG 4240 0.0 21.744022 4 ACGCAGA 13165 0.0 21.601988 7 CGCAGAG 13185 0.0 21.461016 8 GTACTTT 7795 0.0 20.783583 14-15 AGAGTAC 10395 0.0 20.653791 10-11 AGTACTT 7890 0.0 20.218296 12-13 GTCCTAC 595 0.0 20.061605 1 TAAGGTG 1355 0.0 19.991232 5 GCAGAGT 14055 0.0 19.622921 9 >>END_MODULE