FastQCFastQC Report
Thu 26 May 2016
SRR938093_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938093_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2320364
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77420.3336545473037851No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66940.2884892197948253No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41650.17949769949887173No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT25060.10800029650520349No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA23420.10093243991028994No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71200.031.0991841
GTATCAA98950.028.3321341
ATCAACG114950.024.217993
TCAACGC115350.024.129334
TATCAAC116000.024.1268372
CAACGCA115600.024.0771485
AACGCAG119150.023.439516
GTACATG65050.021.8407231
GAGTACT74650.021.56925412-13
CGCAGAG132700.020.9387238
ACGCAGA133150.020.903637
TACATGG65950.020.8942262
GTACTTT77500.020.53091814-15
ACATGGG66300.020.4928323
AGAGTAC104400.019.97235510-11
AGGACCG10500.019.451115
TAAGGTG13750.019.344195
GGACCGA8150.019.2318736
AGTACTT78050.019.16915312-13
GTAAGGT11500.018.9987584
Produced by FastQC (version 0.11.5)