##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938093_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2320364 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.50008964110803 34.0 31.0 34.0 31.0 34.0 2 32.71751587250966 34.0 31.0 34.0 31.0 34.0 3 32.78143644704021 34.0 31.0 34.0 31.0 34.0 4 36.23754074791714 37.0 35.0 37.0 35.0 37.0 5 36.200157389099296 37.0 37.0 37.0 35.0 37.0 6 36.23195929604148 37.0 37.0 37.0 35.0 37.0 7 36.19625886283359 37.0 37.0 37.0 35.0 37.0 8 36.186238883209704 37.0 37.0 37.0 35.0 37.0 9 37.89748849749436 39.0 38.0 39.0 35.0 39.0 10-11 37.88122057573725 39.0 38.0 39.0 35.0 39.0 12-13 37.832059107967545 39.0 38.0 39.0 35.0 39.0 14-15 39.16739011637829 40.5 39.0 41.0 36.0 41.0 16-17 39.12329143186155 40.0 38.0 41.0 36.0 41.0 18-19 39.13842332496108 40.0 39.0 41.0 36.0 41.0 20-21 39.05534131713817 40.0 39.0 41.0 35.5 41.0 22-23 38.91982076949996 40.0 38.0 41.0 35.0 41.0 24-25 38.89143276658317 40.0 38.0 41.0 35.0 41.0 26-27 38.86650068696119 40.0 38.0 41.0 35.0 41.0 28-29 38.81470773551047 40.0 38.0 41.0 35.0 41.0 30-31 38.633995140417625 40.0 38.0 41.0 34.5 41.0 32-33 38.47914615982665 40.0 38.0 41.0 34.0 41.0 34-35 38.29285405220905 40.0 38.0 41.0 34.0 41.0 36-37 38.1744230646571 40.0 38.0 41.0 34.0 41.0 38-39 38.165697063047006 40.0 38.0 41.0 34.0 41.0 40-41 38.11881713386348 40.0 38.0 41.0 33.5 41.0 42-43 37.98086377826927 40.0 38.0 41.0 33.0 41.0 44-45 37.803095333318396 40.0 38.0 41.0 33.0 41.0 46-47 37.52226245537338 40.0 37.0 41.0 33.0 41.0 48-49 37.21961274179396 40.0 37.0 41.0 32.0 41.0 50-51 36.94517907535197 40.0 36.5 41.0 31.0 41.0 52-53 36.54818015621687 39.0 36.0 41.0 30.5 41.0 54-55 36.337468604063844 39.0 35.0 41.0 30.0 41.0 56-57 36.10572112823678 39.0 35.0 40.5 30.0 41.0 58-59 35.78255674540719 38.5 35.0 40.0 28.5 41.0 60-61 35.43064385587779 38.0 35.0 40.0 28.0 41.0 62-63 35.07361323482006 38.0 34.0 40.0 28.0 41.0 64-65 34.68912248250706 37.0 34.0 40.0 27.5 41.0 66-67 34.273745843324576 36.5 34.0 39.0 26.0 41.0 68-69 33.814027454313205 36.0 33.5 39.0 26.0 40.5 70-71 33.297163074414186 35.5 33.0 38.5 25.5 40.0 72-73 32.87827858042962 35.0 32.5 38.0 25.0 40.0 74-75 32.423814539442944 35.0 32.0 37.0 24.0 39.0 76-77 31.122005857701637 34.0 30.5 35.5 22.5 38.5 78-79 31.8279065267346 35.0 32.0 36.0 24.0 39.0 80-81 31.668439089729027 35.0 32.0 36.0 23.0 37.5 82-83 31.30124734739894 35.0 31.5 35.5 21.5 37.0 84-85 30.918092376885696 34.0 31.0 35.0 20.0 36.5 86-87 30.49798738473791 34.0 31.0 35.0 18.5 36.0 88-89 30.047650282455685 34.0 30.5 35.0 16.0 36.0 90-91 29.71322947606496 34.0 30.0 35.0 9.0 35.0 92-93 29.315888800205485 34.0 30.0 35.0 4.5 35.0 94-95 28.91193558424454 34.0 29.0 35.0 2.0 35.0 96-97 28.347053738120398 34.0 29.0 35.0 2.0 35.0 98-99 27.669468454087376 34.0 28.5 35.0 2.0 35.0 100-101 25.236657265842773 31.0 22.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.06057377475949721 1101 2 0.13609902806087604 1101 3 0.11773412718677889 1101 4 0.05642070030545909 1101 5 0.03361413258360102 1101 6 0.09398071271585451 1101 7 0.1416955039775445 1101 8 0.050838038992957024 1101 9 0.11375493308263884 1101 10-11 0.10592067548652295 1101 12-13 0.15507182055340252 1101 14-15 0.1582995311746771 1101 16-17 0.259095672302017 1101 18-19 0.17481259500818425 1101 20-21 0.2286857629081922 1101 22-23 0.1648821128033049 1101 24-25 0.2467308545112914 1101 26-27 0.23346854472695355 1101 28-29 0.2430604556998972 1101 30-31 0.16430278343257498 1101 32-33 0.17004431290105515 1101 34-35 0.09755055794534684 1101 36-37 0.019797574610606716 1101 38-39 0.04041992568741648 1101 40-41 0.15738432688909398 1101 42-43 0.22175566644854428 1101 44-45 0.13942017038961296 1101 46-47 0.3140570409125516 1101 48-49 0.20825166688958063 1101 50-51 0.2302601850761974 1101 52-53 0.3506128951001557 1101 54-55 0.34159328157700486 1101 56-57 0.3398505996732837 1101 58-59 0.28914824694329866 1101 60-61 0.1791323809087757 1101 62-63 0.24319281760805467 1101 64-65 0.3568569428302695 1101 66-67 0.24298822679848087 1101 68-69 0.1854453286017801 1101 70-71 0.3316376453035019 1101 72-73 0.28649571107222727 1101 74-75 0.3999306501360991 1101 76-77 0.21789396932256722 1101 78-79 0.1872842806584778 1101 80-81 0.4563159273572488 1101 82-83 0.5384232559071442 1101 84-85 0.5593031718998134 1101 86-87 0.5795305460738511 1101 88-89 0.5670787556375814 1101 90-91 0.673929473487707 1101 92-93 0.7728955166581777 1101 94-95 0.6056671722108398 1101 96-97 0.7082278579640509 1101 98-99 0.8330378107365526 1101 100-101 0.9421792391316579 1102 1 0.21125077899983324 1102 2 0.20298292622153014 1102 3 0.19952025845666554 1102 4 0.12320412254091195 1102 5 0.1383494739092228 1102 6 0.07745941817894675 1102 7 0.07461065396648081 1102 8 0.08167015771225294 1102 9 0.10539380988898017 1102 10-11 0.13284122828217448 1102 12-13 0.14430372661986013 1102 14-15 0.26252231809703375 1102 16-17 0.2787361719853365 1102 18-19 0.24510122800142398 1102 20-21 0.22701964313579737 1102 22-23 0.15882303295929034 1102 24-25 0.17913281417338567 1102 26-27 0.052208067886020615 1102 28-29 0.18392337855728158 1102 30-31 0.20365309846911117 1102 32-33 0.1459396297054525 1102 34-35 0.2250589584957936 1102 36-37 0.2209510127950196 1102 38-39 0.1260330450451903 1102 40-41 0.05460826610497094 1102 42-43 0.014452211348569222 1102 44-45 0.10482084039870188 1102 46-47 0.07637106506238212 1102 48-49 0.13724507617762072 1102 50-51 0.027014558322633775 1102 52-53 0.03228417857198451 1102 54-55 -0.011082303281909844 1102 56-57 0.07260308041115593 1102 58-59 0.12134645669124922 1102 60-61 0.018192841978809327 1102 62-63 -0.01621058432871081 1102 64-65 -0.0043667267540072885 1102 66-67 0.008397926922356191 1102 68-69 0.21634803483815546 1102 70-71 0.19767780006186797 1102 72-73 0.08256646647313204 1102 74-75 0.08931059921134477 1102 76-77 0.17415896122090047 1102 78-79 0.09459905519813461 1102 80-81 0.20785104687272238 1102 82-83 0.254748558797548 1102 84-85 0.1274519220556165 1102 86-87 0.032785711681068364 1102 88-89 -0.042707726662122525 1102 90-91 0.08767407131692195 1102 92-93 0.08108379582354175 1102 94-95 0.16187900089811436 1102 96-97 0.198907762015903 1102 98-99 0.22347962500107954 1102 100-101 0.49946132719944103 1103 1 0.10560451592563425 1103 2 0.10118146743559464 1103 3 0.09719247209340409 1103 4 0.054983109752271275 1103 5 0.10337668616481466 1103 6 0.07187885273142314 1103 7 0.08232639228089056 1103 8 0.044789899102504194 1103 9 0.014006918058626638 1103 10-11 0.10141835206993477 1103 12-13 0.11405482220243357 1103 14-15 0.15626200618262232 1103 16-17 0.2169132121579409 1103 18-19 0.18513534597345682 1103 20-21 0.20270778844700743 1103 22-23 0.25034430629874294 1103 24-25 0.26709484393143157 1103 26-27 0.19569019141248845 1103 28-29 0.20728709704295056 1103 30-31 0.2105177538925318 1103 32-33 0.1933053788450394 1103 34-35 0.16997747104179695 1103 36-37 0.18576358791801084 1103 38-39 0.20657236283506109 1103 40-41 0.21570183296351075 1103 42-43 0.25245679525115605 1103 44-45 0.17445659185263196 1103 46-47 0.07954022898542235 1103 48-49 0.2305599693816518 1103 50-51 0.1834327685102508 1103 52-53 0.150847864208842 1103 54-55 0.1354010072812244 1103 56-57 0.173066323335604 1103 58-59 0.19931572811949394 1103 60-61 0.13572440296935184 1103 62-63 0.2698764500953459 1103 64-65 0.24936795935318656 1103 66-67 0.2674910757050881 1103 68-69 0.37863610399983827 1103 70-71 0.3203195912180661 1103 72-73 0.30269644325498746 1103 74-75 0.16184488516026363 1103 76-77 0.11851755081230664 1103 78-79 0.18616698878380689 1103 80-81 0.2591045277270929 1103 82-83 0.3137737702617791 1103 84-85 0.36766446880918124 1103 86-87 0.4805057054099322 1103 88-89 0.36294152266783897 1103 90-91 0.4539850002664316 1103 92-93 0.5217375618653328 1103 94-95 0.5524681891591428 1103 96-97 0.5378589889937366 1103 98-99 0.6781277320882104 1103 100-101 0.8922883408690296 1104 1 0.1094561686153881 1104 2 0.04018096241741631 1104 3 0.07499656771966556 1104 4 -6.471315895097973E-4 1104 5 0.05729555606217218 1104 6 -0.058177552819508094 1104 7 0.04191071253354295 1104 8 0.03050713160291707 1104 9 0.08527494045014095 1104 10-11 0.018794533705772665 1104 12-13 0.04657265787948006 1104 14-15 0.10666886589426383 1104 16-17 0.09714032807739414 1104 18-19 0.13932984198666531 1104 20-21 0.13079071100850825 1104 22-23 0.16642771253963673 1104 24-25 0.09831207998830394 1104 26-27 0.026621131955486987 1104 28-29 0.07420413891677669 1104 30-31 0.1337034227570939 1104 32-33 0.21247650887654856 1104 34-35 0.19822114586700224 1104 36-37 0.2016094222293745 1104 38-39 0.16800248894734437 1104 40-41 0.13990287287416692 1104 42-43 0.23616106798942127 1104 44-45 0.1686968951480523 1104 46-47 0.23212329024910616 1104 48-49 0.3081976223986018 1104 50-51 0.34610018914771246 1104 52-53 0.31115556964810764 1104 54-55 0.275856519447899 1104 56-57 0.3513768597218103 1104 58-59 0.42334641330823786 1104 60-61 0.2437591709810718 1104 62-63 0.3618253223399748 1104 64-65 0.24926660007186996 1104 66-67 0.3425609602621975 1104 68-69 0.3569505524986525 1104 70-71 0.46193273307127214 1104 72-73 0.3879484900011789 1104 74-75 0.1957291150786773 1104 76-77 0.2119672517135136 1104 78-79 0.23597885173182576 1104 80-81 0.20781850961756732 1104 82-83 0.353849807505096 1104 84-85 0.3708866270150146 1104 86-87 0.22493627963693186 1104 88-89 0.1645636268153865 1104 90-91 0.1793351343451981 1104 92-93 0.40340326828617634 1104 94-95 0.35710795471690204 1104 96-97 0.4490451415376775 1104 98-99 0.5459961286199473 1104 100-101 0.4986551263216583 1105 1 0.04485835665274607 1105 2 0.06112047850781721 1105 3 0.029524366319392925 1105 4 0.03769327383790255 1105 5 0.04118304822169705 1105 6 0.03835929540723271 1105 7 -0.011739173480073362 1105 8 0.00898496930370385 1105 9 -0.061750173407261855 1105 10-11 0.00663875010373971 1105 12-13 0.03641817740015796 1105 14-15 0.13118017645390267 1105 16-17 0.07697048726031852 1105 18-19 0.0655889806137111 1105 20-21 0.053917711666706225 1105 22-23 0.027037806875988224 1105 24-25 0.07509596873346425 1105 26-27 0.08255074564005582 1105 28-29 -0.020788015075765998 1105 30-31 0.13301371853204103 1105 32-33 0.11184436906505368 1105 34-35 0.14558361224784022 1105 36-37 0.05206853712308401 1105 38-39 0.09942012871793793 1105 40-41 0.08050907553186448 1105 42-43 0.030115662123449738 1105 44-45 -0.07640146924131841 1105 46-47 0.04785893913124539 1105 48-49 0.059849190383943096 1105 50-51 -0.007353786224157943 1105 52-53 -0.10159675375039257 1105 54-55 0.024670220810207866 1105 56-57 0.13557019507468482 1105 58-59 0.007407225209696833 1105 60-61 0.04941201850318322 1105 62-63 0.03552759860346555 1105 64-65 -0.06665441948897666 1105 66-67 -0.04474822402573153 1105 68-69 0.06313655262690077 1105 70-71 0.10224193725984065 1105 72-73 0.055070475700738086 1105 74-75 0.02656860470860778 1105 76-77 -0.0050422954292344 1105 78-79 0.09318741254041285 1105 80-81 0.02299122865532155 1105 82-83 0.293146438181104 1105 84-85 0.2677073908896226 1105 86-87 0.3523987061428038 1105 88-89 0.15132786553154887 1105 90-91 0.05753775371028169 1105 92-93 0.15687076871551398 1105 94-95 0.02588998459585312 1105 96-97 0.18673715497281407 1105 98-99 0.16340542044499173 1105 100-101 0.1900787289105068 1106 1 -0.1484349262320137 1106 2 -0.0565473432698127 1106 3 0.00914617181511801 1106 4 -0.018231992675829645 1106 5 0.013901421135088299 1106 6 -0.029319384271936144 1106 7 0.013234353753453831 1106 8 -0.1793288870055889 1106 9 -0.12545139508033998 1106 10-11 -0.07968245138142294 1106 12-13 -0.12311976406903113 1106 14-15 -0.2737113258764694 1106 16-17 -0.10593952938375395 1106 18-19 -0.0911002648293362 1106 20-21 0.022422723631613906 1106 22-23 -0.09185179408375888 1106 24-25 -0.03354352559248497 1106 26-27 -0.07074460979530528 1106 28-29 0.05984888042737424 1106 30-31 0.033609983555379586 1106 32-33 -0.018242840751341305 1106 34-35 -0.05155131645430089 1106 36-37 -0.017977660241733417 1106 38-39 -0.06269647594904626 1106 40-41 -0.03792746445456885 1106 42-43 -0.06314133344213246 1106 44-45 -0.053246552097363065 1106 46-47 -0.18919274801688601 1106 48-49 -0.30885260778357804 1106 50-51 -0.21644071804997367 1106 52-53 -0.4013850069863665 1106 54-55 -0.6255744887037906 1106 56-57 -0.4077026098784984 1106 58-59 -0.26941656625634636 1106 60-61 -0.3573805007916988 1106 62-63 -0.4426388236719987 1106 64-65 -0.5492777097231496 1106 66-67 -0.5804282788033177 1106 68-69 -0.6504028261879498 1106 70-71 -0.5698156598405717 1106 72-73 -0.4735135690550436 1106 74-75 -0.4856900368724162 1106 76-77 -0.4614037878637198 1106 78-79 -0.4177247785133851 1106 80-81 -0.5855888478380642 1106 82-83 -0.6707215071007333 1106 84-85 -0.5084115211698617 1106 86-87 -0.3029566443269154 1106 88-89 -0.29693016630933755 1106 90-91 -0.3254646435163231 1106 92-93 -0.24947505580240303 1106 94-95 -0.5474694642896019 1106 96-97 -0.4909262316880252 1106 98-99 -0.5616385593614375 1106 100-101 -0.534232607799737 1107 1 0.11472762159711891 1107 2 0.09301551333321356 1107 3 0.04103849298441986 1107 4 -0.02565731759062828 1107 5 0.013295189681983288 1107 6 -0.027779051188169035 1107 7 0.04581929435792631 1107 8 0.024123904235580085 1107 9 -0.07587075568802248 1107 10-11 0.05482692045165294 1107 12-13 0.0037614479088858843 1107 14-15 0.1267810856213103 1107 16-17 0.09916726333863579 1107 18-19 0.06968444116356665 1107 20-21 0.08332473541069163 1107 22-23 0.10587312031131546 1107 24-25 0.06899218901799742 1107 26-27 0.054758183416119266 1107 28-29 0.14144990738345342 1107 30-31 0.08190642265286385 1107 32-33 0.0029765230895719696 1107 34-35 0.0811295097583411 1107 36-37 0.07131467567919714 1107 38-39 0.07689463103969274 1107 40-41 0.1593154959352816 1107 42-43 0.19756244065177242 1107 44-45 0.2144351986182471 1107 46-47 0.32953778311259185 1107 48-49 0.2586320671904758 1107 50-51 0.28436986392211594 1107 52-53 0.2913094493301145 1107 54-55 0.33959877729743226 1107 56-57 0.2866420589726175 1107 58-59 0.30198613207985403 1107 60-61 0.3445587325882187 1107 62-63 0.4999230195141138 1107 64-65 0.45902084108055874 1107 66-67 0.4660421406381161 1107 68-69 0.5134501687469779 1107 70-71 0.47801398103801773 1107 72-73 0.4829246594962413 1107 74-75 0.4651812187642719 1107 76-77 0.41687327203498015 1107 78-79 0.41350190052041214 1107 80-81 0.4771182180926381 1107 82-83 0.41633737110838 1107 84-85 0.5420698771231933 1107 86-87 0.4970177929134785 1107 88-89 0.5933492659751138 1107 90-91 0.575032062120922 1107 92-93 0.5486690676424857 1107 94-95 0.5366539907042807 1107 96-97 0.7640231429337128 1107 98-99 0.8272884715897142 1107 100-101 0.7626146855283835 1108 1 0.09219490034799094 1108 2 0.09239897220323456 1108 3 0.11603217900898954 1108 4 0.06588344097780663 1108 5 0.06468352145539313 1108 6 0.03775258554282601 1108 7 0.0583878195618297 1108 8 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-0.54308531154728 2316 60-61 -0.43541649593188936 2316 62-63 -0.5967061140196179 2316 64-65 -0.6803227609456144 2316 66-67 -0.49467456752069694 2316 68-69 -0.4631047129992609 2316 70-71 -0.6279773279107843 2316 72-73 -0.37622211920446347 2316 74-75 -0.3764402525566126 2316 76-77 -0.3715223353309476 2316 78-79 -0.2839158002429194 2316 80-81 -0.470495606380112 2316 82-83 -0.5154271664730103 2316 84-85 -0.4020724197088299 2316 86-87 -0.48614629748049865 2316 88-89 -0.6087804010717903 2316 90-91 -0.5501318591121525 2316 92-93 -0.7342347775159261 2316 94-95 -0.6867724959835435 2316 96-97 -0.6581247320310233 2316 98-99 -0.6526158406258098 2316 100-101 -0.7491191733672657 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 9.0 4 14.0 5 35.0 6 67.0 7 165.0 8 418.0 9 864.0 10 1487.0 11 2368.0 12 3560.0 13 5212.0 14 7201.0 15 9692.0 16 12037.0 17 16586.0 18 16834.0 19 12370.0 20 10575.0 21 10827.0 22 11257.0 23 12327.0 24 14291.0 25 16571.0 26 19901.0 27 24554.0 28 29862.0 29 36773.0 30 45865.0 31 56502.0 32 70828.0 33 92635.0 34 129493.0 35 194923.0 36 326079.0 37 553657.0 38 512320.0 39 62195.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.8712908836717 18.939183679801964 14.791213792318791 23.39831164420755 2 15.763314507240711 19.79675061325246 40.12091342827845 24.31902145122838 3 19.621395849594453 23.335288618644803 29.739243093396407 27.304072438364337 4 13.077732631604352 16.677469569429622 36.65709345602673 33.5877043429393 5 13.768615613757152 37.21187710204089 34.47359983175053 14.545907452451425 6 32.9593115562903 36.03245008110797 16.742416276067033 14.2658220865347 7 28.903956448212437 32.403278106366066 20.20027892175538 18.492486523666116 8 26.153777198939647 35.36516148161684 19.291988258703295 19.18907306074022 9 25.731082361753195 16.989899416513207 20.1718180920986 37.107200129635 10-11 25.153359559103656 26.348430677255806 27.02597523492004 21.472234528720495 12-13 26.002859896119745 23.57390909357325 27.446641992377057 22.976589017929946 14-15 22.680191556152394 25.18958232415259 25.76940514505483 26.360820974640188 16-17 22.290063735827083 28.350311309586147 26.682896913796526 22.676728040790238 18-19 22.25530132341305 27.050863571405177 28.68246102766635 22.011374077515423 20-21 22.645951238684965 26.09023842810869 29.203370678048792 22.060439655157552 22-23 22.5329086298529 25.967865386637612 29.01665428355206 22.482571699957422 24-25 22.555715165549756 26.242921526618595 28.88428735143233 22.317075956399318 26-27 22.014882749743844 26.52703797243549 28.971682428346107 22.48639684947456 28-29 22.18380870066263 26.989435060498064 28.756378041630114 22.070378197209187 30-31 22.288442675373346 26.364591934713694 28.701746794899417 22.645218595013542 32-33 22.05134083979804 26.307757582835904 28.881759713112924 22.75914186425313 34-35 22.792558591030705 26.485503701178093 28.5070025608454 22.2149351469458 36-37 22.77087440233558 26.100361938764337 28.631605985824137 22.497157673075947 38-39 22.189620937869552 26.557991887323407 28.597777667143188 22.654609507663853 40-41 22.777662118004866 26.351872373679054 28.487799474401765 22.38266603391432 42-43 22.358813694977844 26.268835292449417 28.438387727843413 22.933963284729327 44-45 22.480469145624514 26.451157456590803 28.624903730245578 22.44346966753911 46-47 22.84316659868163 26.84771157622502 27.858436835435484 22.45068498965787 48-49 22.560986121143063 26.67482343287519 28.028425712517517 22.73576473346423 50-51 22.59509714855083 26.954714863702417 28.006317112315138 22.443870875431614 52-53 22.871152974274725 26.832708144067052 27.781804923710247 22.514333957947976 54-55 22.504141591577874 26.85910486458159 27.915814070550997 22.720939473289537 56-57 22.60854331475579 26.978504234680422 27.832206498635557 22.580745951928233 58-59 22.69441346271533 26.69600545431665 27.766096181461187 22.843484901506834 60-61 22.359035047949373 26.990916080408073 27.960095915985594 22.68995295565696 62-63 22.335008645195323 26.97964629687411 28.09356635424442 22.591778703686145 64-65 22.048695808071493 27.09204245540786 27.930466943979482 22.928794792541172 66-67 22.196582087982748 26.969044511981743 27.751766533181865 23.08260686685365 68-69 22.42404457071809 26.761802868738975 27.977348318093227 22.83680424244971 70-71 22.457834201875222 26.883777717633954 27.554620740538983 23.103767339951837 72-73 22.415319320589354 26.820835006921328 27.70362753430065 23.060218138188663 74-75 22.279543209599872 27.402941952210945 27.3541780513747 22.963336786814484 76-77 22.54866046878852 26.88623422876756 27.536994195738256 23.028111106705673 78-79 22.478692998167528 27.083617053186483 27.573626379309452 22.864063569336533 80-81 22.641512279969866 27.144081704422234 27.338964921021013 22.875441094586886 82-83 22.618723880703147 27.131832390018285 27.37050194301495 22.878941786263614 84-85 22.466153644257005 27.282984560411656 27.308196801148473 22.942664994182866 86-87 22.630655541367126 27.284206697503876 27.135738164429007 22.94939959669999 88-89 22.50188871310222 27.44641678909722 26.91684447648924 23.13485002131132 90-91 22.486848476216068 27.30157861382196 27.06730447040834 23.144268439553628 92-93 22.568921447069183 27.0627519276112 27.27649040514851 23.091836220171103 94-95 22.323544926571863 27.06512857465467 27.311857105178326 23.299469393595142 96-97 22.385194736687865 26.820511781772172 27.391650620333706 23.40264286120626 98-99 22.227288477152722 27.083099892947832 27.039270562722056 23.65034106717739 100-101 22.404803729070093 26.8099099968798 27.238549641349373 23.546736632700732 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 602.0 1 398.5 2 297.5 3 390.5 4 306.0 5 301.5 6 610.0 7 1396.5 8 2321.0 9 2748.0 10 2341.5 11 1806.0 12 1773.5 13 1875.5 14 1992.5 15 2133.5 16 2297.5 17 2485.0 18 2657.0 19 2933.5 20 3411.0 21 4060.5 22 4877.5 23 6041.5 24 7821.0 25 10169.0 26 12994.0 27 16483.0 28 19764.0 29 22656.0 30 25869.0 31 29847.0 32 35915.0 33 39400.0 34 40498.5 35 43819.0 36 49084.5 37 53945.0 38 57259.5 39 60460.0 40 63733.0 41 68998.5 42 73265.5 43 75529.5 44 79171.0 45 83227.5 46 85298.5 47 89815.5 48 94207.5 49 92173.5 50 88690.5 51 85187.5 52 83289.0 53 84207.0 54 85572.0 55 81336.0 56 73560.5 57 66811.0 58 60558.5 59 52478.0 60 41313.5 61 33615.5 62 28073.5 63 20910.0 64 14841.0 65 11072.0 66 8094.0 67 5894.0 68 4286.0 69 2964.0 70 2073.0 71 1391.0 72 943.0 73 712.5 74 506.0 75 344.0 76 229.0 77 117.0 78 55.5 79 32.0 80 18.0 81 6.0 82 6.0 83 6.0 84 4.0 85 3.0 86 0.5 87 1.5 88 1.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.015385517099903292 3 0.0067661797890331 4 0.0 5 0.0 6 0.0 7 0.0 8 3.016768058804567E-4 9 1.2929005966305285E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.1548343277175478E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0053870858192938695 26-27 0.021419053217512425 28-29 2.154834327717548E-5 30-31 0.0 32-33 3.6632183571198314E-4 34-35 0.018402285158707857 36-37 0.0035339282974567784 38-39 0.01786357657677847 40-41 0.014695970115033676 42-43 0.007972887012554927 44-45 0.0026288978798154083 46-47 2.154834327717548E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.801284626032812E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 4.309668655435096E-5 84-85 0.0026935429096469348 86-87 3.016768058804567E-4 88-89 4.309668655435096E-5 90-91 2.3703177604893026E-4 92-93 2.1548343277175478E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2320364.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.103753959981034 #Duplication Level Percentage of deduplicated Percentage of total 1 72.52774139691181 37.064398516209124 2 14.880712491004347 15.209205397790054 3 5.331430522492974 8.173683410286424 4 2.476150442111715 5.061623318463013 5 1.3298890508175272 3.3981161423525807 6 0.7808134562153081 2.3941499253041716 7 0.4983263703011666 1.7826443753748855 8 0.3461397090074539 1.4151230819917084 9 0.2593746597947665 1.1929516917844978 >10 1.426332927838223 13.113226194236557 >50 0.09274542743714022 3.189557435868482 >100 0.04409787615700904 4.198140620374689 >500 0.0037980425132537504 1.3388619994150872 >1k 0.0022788255079519326 1.845663600301697 >5k 1.6880188947792092E-4 0.6226542902469048 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7742 0.3336545473037851 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6694 0.2884892197948253 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4165 0.17949769949887173 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2506 0.10800029650520349 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2342 0.10093243991028994 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.619337310870191E-5 2 0.0 0.0 0.0 0.0 8.619337310870191E-5 3 0.0 0.0 0.0 0.0 8.619337310870191E-5 4 0.0 0.0 0.0 0.0 8.619337310870191E-5 5 0.0 0.0 0.0 0.0 8.619337310870191E-5 6 0.0 0.0 0.0 0.0 8.619337310870191E-5 7 0.0 0.0 0.0 0.0 8.619337310870191E-5 8 0.0 0.0 0.0 0.0 8.619337310870191E-5 9 0.0 0.0 0.0 0.0 8.619337310870191E-5 10-11 0.0 0.0 0.0 8.619337310870191E-5 1.2929005966305288E-4 12-13 0.0 0.0 0.0 1.2929005966305288E-4 2.1548343277175478E-4 14-15 0.0 0.0 0.0 1.2929005966305288E-4 2.1548343277175478E-4 16-17 0.0 0.0 0.0 2.1548343277175478E-4 2.5858011932610575E-4 18-19 0.0 0.0 0.0 3.4477349243480765E-4 3.4477349243480765E-4 20-21 0.0 0.0 0.0 3.4477349243480765E-4 3.878701789891586E-4 22-23 0.0 0.0 0.0 4.0941852226633406E-4 3.878701789891586E-4 24-25 0.0 0.0 0.0 4.7406355209786053E-4 3.878701789891586E-4 26-27 0.0 4.309668655435096E-5 0.0 0.0010127721340272475 4.5251520882068504E-4 28-29 0.0 4.309668655435096E-5 0.0 0.004589797118038377 4.7406355209786053E-4 30-31 0.0 4.309668655435096E-5 0.0 0.014782163488142378 5.171602386522115E-4 32-33 0.0 4.309668655435096E-5 0.0 0.037537213988839684 5.171602386522115E-4 34-35 0.0 4.309668655435096E-5 0.0 0.06742476611428208 5.171602386522115E-4 36-37 0.0 4.309668655435096E-5 0.0 0.11670582718918238 5.171602386522115E-4 38-39 0.0 4.309668655435096E-5 0.0 0.19089677309249756 5.602569252065625E-4 40-41 0.0 4.309668655435096E-5 0.0 0.26911725918864454 6.033536117609134E-4 42-43 0.0 4.309668655435096E-5 0.0 0.3469714234490795 6.249019550380888E-4 44-45 0.0 4.309668655435096E-5 0.0 0.4408360067644559 6.464502983152643E-4 46-47 0.0 4.309668655435096E-5 0.0 0.5515729428658607 6.679986415924398E-4 48-49 0.0 4.309668655435096E-5 0.0 0.666059290697494 6.895469848696153E-4 50-51 0.0 4.309668655435096E-5 0.0 0.7859327243484211 6.895469848696153E-4 52-53 0.0 4.309668655435096E-5 0.0 0.9003544271502231 7.326436714239663E-4 54-55 0.0 4.309668655435096E-5 0.0 1.02671391212758 7.757403579783172E-4 56-57 0.0 4.309668655435096E-5 0.0 1.1692562029060958 7.757403579783172E-4 58-59 0.0 4.309668655435096E-5 0.0 1.3152246802656826 7.757403579783172E-4 60-61 0.0 4.309668655435096E-5 0.0 1.4723982961294002 7.972887012554926E-4 62-63 0.0 4.309668655435096E-5 0.0 1.6298520404557215 8.619337310870191E-4 64-65 0.0 4.309668655435096E-5 0.0 1.7882539118862386 8.834820743641946E-4 66-67 0.0 4.309668655435096E-5 0.0 1.9704020576082026 9.265787609185456E-4 68-69 0.0 4.309668655435096E-5 0.0 2.17526215714431 9.481271041957211E-4 70-71 0.0 4.309668655435096E-5 0.0 2.382061607575363 9.696754474728965E-4 72-73 0.0 4.309668655435096E-5 0.0 2.587740544156003 0.0010558688205815985 74-75 0.0 4.309668655435096E-5 0.0 2.8001425638391217 0.0011636105369674757 76-77 0.0 4.309668655435096E-5 0.0 3.0253012027423285 0.0012067072235218267 78-79 0.0 4.309668655435096E-5 0.0 3.2822220996360914 0.0012498039100761777 80-81 0.0 4.309668655435096E-5 0.0 3.5501326516012144 0.0012498039100761777 82-83 0.0 4.309668655435096E-5 0.0 3.8198748127448967 0.0012498039100761777 84-85 0.0 4.309668655435096E-5 0.0 4.099119793273815 0.0012498039100761777 86-87 0.0 4.309668655435096E-5 0.0 4.386962562770324 0.0013575456264620551 88-89 0.0 4.309668655435096E-5 0.0 4.6929490373062155 0.0014652873428479326 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7120 0.0 31.099184 1 GTATCAA 9895 0.0 28.332134 1 ATCAACG 11495 0.0 24.21799 3 TCAACGC 11535 0.0 24.12933 4 TATCAAC 11600 0.0 24.126837 2 CAACGCA 11560 0.0 24.077148 5 AACGCAG 11915 0.0 23.43951 6 GTACATG 6505 0.0 21.840723 1 GAGTACT 7465 0.0 21.569254 12-13 CGCAGAG 13270 0.0 20.938723 8 ACGCAGA 13315 0.0 20.90363 7 TACATGG 6595 0.0 20.894226 2 GTACTTT 7750 0.0 20.530918 14-15 ACATGGG 6630 0.0 20.492832 3 AGAGTAC 10440 0.0 19.972355 10-11 AGGACCG 1050 0.0 19.45111 5 TAAGGTG 1375 0.0 19.34419 5 GGACCGA 815 0.0 19.231873 6 AGTACTT 7805 0.0 19.169153 12-13 GTAAGGT 1150 0.0 18.998758 4 >>END_MODULE