FastQCFastQC Report
Thu 26 May 2016
SRR938092_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938092_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2221954
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70390.3167932369436991No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60230.27106771787354733No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37820.1702105444127106No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT23880.10747297198771891No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA23540.10594278729442644No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA94300.031.1011091
GGTATCA65450.029.606811
GGACCGA6700.026.2582056
TCAACGC110700.025.8633654
CAACGCA111250.025.6927495
ATCAACG111250.025.6927493
TATCAAC112100.025.6252142
GTACATG62050.025.5510831
AACGCAG116400.024.591316
TACATGG62200.024.1619992
TAAGGTG12450.024.0661895
GTAAGGT11550.023.470864
ACATGGG65100.022.2820433
GAGTACT71400.022.2058412-13
CGCAGAG126600.022.1938748
ACGCAGA127700.022.1526177
AGAGTAC100600.021.14753310-11
AGGACCG9100.020.905225
GTACTTT74950.020.89774514-15
ACCGAGT8100.020.542938
Produced by FastQC (version 0.11.5)