FastQCFastQC Report
Thu 26 May 2016
SRR938092_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938092_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2221954
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70090.31544307397902926No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62790.2825891085053966No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37630.16935544120175305No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT24200.10891314581670007No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA22660.10198230926472825No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61650.031.5970461
GTATCAA87750.030.8619541
TCAACGC102200.026.0223854
ATCAACG102650.025.958663
TATCAAC103600.025.7264142
CAACGCA104000.025.5719995
AACGCAG109050.024.4307826
TAAGGTG11900.022.747725
GTAAGGT10850.022.322894
ACGCAGA120600.022.0516417
CGCAGAG120400.022.0093848
GTACATG60050.021.836941
TACATGG60850.021.314652
GAGTACT73300.021.02473312-13
ACATGGG60300.020.8740623
GTACTTT73800.020.56006414-15
GTGTAAG10050.020.3281611
GCAGAGT128850.020.2711549
ACGACCG600.00476967919.78874646-47
AGAGTAC99900.019.70488510-11
Produced by FastQC (version 0.11.5)