##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938092_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2221954 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.51172526523951 34.0 31.0 34.0 31.0 34.0 2 32.72826800194783 34.0 31.0 34.0 31.0 34.0 3 32.78905008834566 34.0 31.0 34.0 31.0 34.0 4 36.242810607240294 37.0 35.0 37.0 35.0 37.0 5 36.21362728481328 37.0 37.0 37.0 35.0 37.0 6 36.24011523190849 37.0 37.0 37.0 35.0 37.0 7 36.19931555738778 37.0 37.0 37.0 35.0 37.0 8 36.18180619400761 37.0 37.0 37.0 35.0 37.0 9 37.87324580076815 39.0 38.0 39.0 35.0 39.0 10-11 37.8479824514819 39.0 38.0 39.0 35.0 39.0 12-13 37.773851978933855 39.0 38.0 39.0 35.0 39.0 14-15 39.06458054487177 40.0 38.0 41.0 36.0 41.0 16-17 38.94887292896252 40.0 38.0 41.0 36.0 41.0 18-19 39.19021770927751 40.5 39.0 41.0 36.0 41.0 20-21 39.16325270460145 40.0 39.0 41.0 36.0 41.0 22-23 39.07467301303267 40.0 39.0 41.0 35.5 41.0 24-25 38.985255320317165 40.0 38.5 41.0 35.0 41.0 26-27 38.82172943274253 40.0 38.0 41.0 35.0 41.0 28-29 38.71058446754523 40.0 38.0 41.0 34.5 41.0 30-31 38.487515268092864 40.0 38.0 41.0 34.0 41.0 32-33 38.28662317041667 40.0 38.0 41.0 34.0 41.0 34-35 38.145328616163965 40.0 38.0 41.0 34.0 41.0 36-37 38.151968042542734 40.0 38.0 41.0 34.0 41.0 38-39 38.012245753062395 40.0 38.0 41.0 33.0 41.0 40-41 37.85391214219557 40.0 37.5 41.0 33.0 41.0 42-43 37.64428809057253 40.0 37.0 41.0 33.0 41.0 44-45 37.39650033259014 40.0 37.0 41.0 32.5 41.0 46-47 37.042372839401715 40.0 36.5 41.0 31.0 41.0 48-49 36.6818883289213 39.5 36.0 41.0 30.5 41.0 50-51 36.31912654357381 39.0 35.5 41.0 30.0 41.0 52-53 35.81509698220575 39.0 35.0 40.0 28.5 41.0 54-55 35.51533875138729 38.0 35.0 40.0 28.0 41.0 56-57 35.17610760618807 38.0 34.0 40.0 27.0 41.0 58-59 34.7432563860458 38.0 34.0 40.0 26.0 41.0 60-61 34.26812976326242 37.0 33.5 40.0 26.0 41.0 62-63 33.805782657966816 37.0 33.0 39.5 24.5 41.0 64-65 33.31823003536527 36.0 32.5 39.0 23.0 40.5 66-67 32.770629139937185 35.5 32.0 39.0 22.0 40.0 68-69 32.17853654936151 35.0 31.0 38.0 19.5 40.0 70-71 31.565291855726986 35.0 30.5 37.5 18.0 40.0 72-73 30.973971558367097 34.5 30.0 37.0 14.0 39.0 74-75 30.372113239067957 34.0 29.0 36.0 9.0 39.0 76-77 29.020054645595724 32.5 28.0 35.0 7.5 37.0 78-79 29.692933787108103 34.0 29.0 35.0 7.0 37.0 80-81 29.370909568784953 34.0 29.0 35.0 2.0 37.0 82-83 28.802034830604054 34.0 28.5 35.0 2.0 36.5 84-85 28.2058507961911 33.0 27.0 35.0 2.0 36.0 86-87 27.564670330708918 33.0 26.0 35.0 2.0 35.5 88-89 26.819852931248803 32.0 25.0 35.0 2.0 35.0 90-91 26.275036071853876 32.0 24.5 34.5 2.0 35.0 92-93 25.457167430108814 32.0 21.5 34.0 2.0 35.0 94-95 24.631663166744225 31.0 18.0 34.0 2.0 35.0 96-97 23.572389437405093 31.0 4.5 34.0 2.0 35.0 98-99 22.127299215015253 30.5 2.0 34.0 2.0 35.0 100-101 17.72175436575195 23.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.10136985553396727 1101 2 0.11861460319055084 1101 3 0.15028046466009926 1101 4 0.0958441774561507 1101 5 0.07438019163631537 1101 6 0.0949526060915531 1101 7 0.15132653941664387 1101 8 0.0622052865473961 1101 9 0.08307722137699614 1101 10-11 0.18009316920139185 1101 12-13 0.2914916122284197 1101 14-15 0.2483449563964868 1101 16-17 0.33051326642479495 1101 18-19 0.3034213057146147 1101 20-21 0.28321768506582146 1101 22-23 0.2530191040939158 1101 24-25 0.31075124745118643 1101 26-27 0.3261142967749464 1101 28-29 0.3242031521915152 1101 30-31 0.4360055317157858 1101 32-33 0.3719413371251221 1101 34-35 0.30519417467758103 1101 36-37 0.31417339726914406 1101 38-39 0.3765109727338114 1101 40-41 0.3203764496309631 1101 42-43 0.3692124257431004 1101 44-45 0.4162835147498498 1101 46-47 0.4127997909144625 1101 48-49 0.42149532834402237 1101 50-51 0.11447450105403334 1101 52-53 -0.004183964115171079 1101 54-55 -0.028760413333287715 1101 56-57 0.07376598673037194 1101 58-59 0.21435711900021914 1101 60-61 0.12698657691309734 1101 62-63 0.05107915055203449 1101 64-65 0.1103388751044676 1101 66-67 0.16011143714231224 1101 68-69 0.14269959269421406 1101 70-71 0.18453350528793067 1101 72-73 0.029630145209029735 1101 74-75 0.09393016928420295 1101 76-77 0.02203670382946399 1101 78-79 -0.1407880858166699 1101 80-81 0.046350009379228396 1101 82-83 0.036156542382769885 1101 84-85 0.05693427888740388 1101 86-87 -0.15573793506587563 1101 88-89 -0.4008117064349044 1101 90-91 -0.4224068926375786 1101 92-93 -0.6744654503405201 1101 94-95 -0.9848420298714835 1101 96-97 -0.8434765970477827 1101 98-99 -0.7481784846306141 1101 100-101 -1.1808991638922066 1102 1 0.10680684129016527 1102 2 0.11547985627485247 1102 3 0.08213419498116536 1102 4 0.09428883579927572 1102 5 0.09570008020332921 1102 6 0.03963316177565446 1102 7 0.08049949192805173 1102 8 0.13484959874486435 1102 9 0.0732768567208737 1102 10-11 0.18750591550624307 1102 12-13 0.17710690001983664 1102 14-15 0.18887546626719143 1102 16-17 0.14643018221145354 1102 18-19 0.14568809701840024 1102 20-21 0.12573668142852057 1102 22-23 0.1602425809141863 1102 24-25 0.13166066650030217 1102 26-27 0.1764502616791077 1102 28-29 0.0439135559972641 1102 30-31 0.08664252302511954 1102 32-33 0.14419299612537628 1102 34-35 0.24447731072022805 1102 36-37 0.29247635801770855 1102 38-39 0.17690570835812025 1102 40-41 0.22679125059720917 1102 42-43 0.2884635386846952 1102 44-45 0.19040542465941712 1102 46-47 0.2641350894419574 1102 48-49 0.29987765270286104 1102 50-51 0.2533837257432836 1102 52-53 0.33174785259423345 1102 54-55 0.23536931724692778 1102 56-57 0.3139623827432132 1102 58-59 0.30728080036110583 1102 60-61 0.30176190616795395 1102 62-63 0.3312155726646324 1102 64-65 0.18042133921731107 1102 66-67 0.11211542614709913 1102 68-69 0.26645175610457983 1102 70-71 0.24805053954154488 1102 72-73 0.18257701092487721 1102 74-75 0.007166075880405742 1102 76-77 -0.009435479572200478 1102 78-79 0.167578412096276 1102 80-81 -0.003345030742199384 1102 82-83 -0.20176144701073184 1102 84-85 -0.1946483657024487 1102 86-87 -0.4734170469338643 1102 88-89 -0.3847420793281806 1102 90-91 -0.6152044714690135 1102 92-93 -0.48073193622166244 1102 94-95 -0.2742989328859089 1102 96-97 -0.6485058898555423 1102 98-99 -0.7524340400833367 1102 100-101 -1.227295870880834 1103 1 0.11187780214727638 1103 2 0.12597005213441292 1103 3 0.13107899129659728 1103 4 0.10606563672264713 1103 5 0.11220447939693656 1103 6 0.037445955492430016 1103 7 0.08348929151414097 1103 8 0.08425116999706717 1103 9 0.1715267791405779 1103 10-11 0.12535873842446676 1103 12-13 0.1390888701352324 1103 14-15 0.20726244349319245 1103 16-17 0.14240192289009457 1103 18-19 0.26345063656202683 1103 20-21 0.30390502732725366 1103 22-23 0.23360105993848634 1103 24-25 0.2357370367693008 1103 26-27 0.2301740043759537 1103 28-29 0.22746251418874408 1103 30-31 0.2601301537116427 1103 32-33 0.2462023816670822 1103 34-35 0.18093313013562096 1103 36-37 0.17644346843623993 1103 38-39 0.20057249930209764 1103 40-41 0.2330273670725873 1103 42-43 0.19647931371746097 1103 44-45 0.22427787595127313 1103 46-47 0.11757742216432376 1103 48-49 0.11416091120935334 1103 50-51 0.06147149975801369 1103 52-53 0.04566653951140154 1103 54-55 -0.05741426385964843 1103 56-57 0.10287855806799939 1103 58-59 0.16349481482340877 1103 60-61 0.08263674403071519 1103 62-63 0.0519477074288659 1103 64-65 0.03752334112388667 1103 66-67 0.005357679838027707 1103 68-69 0.04558265583511911 1103 70-71 -0.1577023465571834 1103 72-73 -0.45217449770495577 1103 74-75 -0.34706366896413243 1103 76-77 -0.1622434398689485 1103 78-79 -0.23375038771976264 1103 80-81 -0.13094586838616706 1103 82-83 -0.32210668491993033 1103 84-85 -0.46873476001575654 1103 86-87 -0.4522165280946844 1103 88-89 -0.4874928869446684 1103 90-91 -0.6182062150722665 1103 92-93 -0.6511514056393963 1103 94-95 -0.5081816537999977 1103 96-97 -0.6704439238479907 1103 98-99 -0.967556170449491 1103 100-101 -1.7532832615707505 1104 1 0.13504988697348352 1104 2 0.10447932394152559 1104 3 0.07718358271013415 1104 4 0.05224283413124198 1104 5 -0.007410342567560235 1104 6 0.059450958196300974 1104 7 -0.035327295400833236 1104 8 0.061388037683947516 1104 9 0.1434803974829606 1104 10-11 0.0670093721649252 1104 12-13 0.12523706823541403 1104 14-15 0.16195996519419253 1104 16-17 -0.018667475083397278 1104 18-19 0.0178772323319194 1104 20-21 0.05705143074511909 1104 22-23 0.07940574765729735 1104 24-25 0.12183859036523614 1104 26-27 0.17340710409177973 1104 28-29 -0.01601888941225127 1104 30-31 -0.013116260694076232 1104 32-33 -0.030748662071026445 1104 34-35 -0.05366136430474455 1104 36-37 -0.11363762621224538 1104 38-39 0.08059766363510334 1104 40-41 0.04897569401070001 1104 42-43 -0.11433564010125252 1104 44-45 -0.17510411129850922 1104 46-47 -0.10844934941118112 1104 48-49 -0.15760386691147232 1104 50-51 -0.19863743077937812 1104 52-53 -0.13741540184794587 1104 54-55 -0.16632594954679547 1104 56-57 -0.28442347209904284 1104 58-59 -0.1847405578694108 1104 60-61 -0.37644676148151746 1104 62-63 -0.2993446577383523 1104 64-65 -0.3328078456286434 1104 66-67 -0.37396717058716433 1104 68-69 -0.37344754782793643 1104 70-71 -0.5878785399778295 1104 72-73 -0.6920131055657919 1104 74-75 -0.56733625384528 1104 76-77 -0.6739549807749974 1104 78-79 -0.732849383947265 1104 80-81 -0.6550210037414104 1104 82-83 -0.6310264499091893 1104 84-85 -0.8830202588534135 1104 86-87 -0.853977277336849 1104 88-89 -0.8041501457128639 1104 90-91 -0.8067067827778942 1104 92-93 -0.7368579352236466 1104 94-95 -0.7478787122876795 1104 96-97 -1.057761035378988 1104 98-99 -0.951161219690146 1104 100-101 -1.5284248648059169 1105 1 0.18492956666314342 1105 2 0.1910240484490089 1105 3 0.10784332362904792 1105 4 0.09612091778980414 1105 5 0.0876752494409132 1105 6 0.09564304387606626 1105 7 0.15450894455753428 1105 8 0.14564912760756954 1105 9 0.10884994212795363 1105 10-11 0.16684082897383234 1105 12-13 0.17010206270862938 1105 14-15 0.2569221676017577 1105 16-17 0.1571956650439219 1105 18-19 0.22060377172533663 1105 20-21 0.2054327816532009 1105 22-23 0.22320777882728038 1105 24-25 0.2514535983309756 1105 26-27 0.286573907468906 1105 28-29 0.21165418458731722 1105 30-31 0.20136483762581037 1105 32-33 0.2671357727531003 1105 34-35 0.3108282209125619 1105 36-37 0.24573788233212213 1105 38-39 0.28815297987296873 1105 40-41 0.15180310800774066 1105 42-43 0.21183467627462704 1105 44-45 0.07169332087943303 1105 46-47 0.15460986400173482 1105 48-49 0.21180430794828453 1105 50-51 0.18511535166642545 1105 52-53 0.14161112629827244 1105 54-55 0.3054659311290848 1105 56-57 0.19629695425631866 1105 58-59 0.2821090440085854 1105 60-61 0.050247765005217104 1105 62-63 0.049271751389433405 1105 64-65 0.05064470252111164 1105 66-67 -0.049523278660799974 1105 68-69 -0.11533370310272772 1105 70-71 -0.09981653536441826 1105 72-73 -0.20332673721219763 1105 74-75 -0.24864501582529996 1105 76-77 -0.13968171668540563 1105 78-79 -0.11356068482797 1105 80-81 -0.19140558955815834 1105 82-83 -0.04994176674031081 1105 84-85 -0.26992040308947196 1105 86-87 -0.5898884096331578 1105 88-89 -0.6591196773391239 1105 90-91 -0.8356832547087194 1105 92-93 -0.581743199374209 1105 94-95 -0.856751907740513 1105 96-97 -0.9380520082452222 1105 98-99 -0.9270394796784203 1105 100-101 -1.2712764046628209 1106 1 0.2541394799789103 1106 2 0.16520901878473637 1106 3 0.22590074513475145 1106 4 0.12872719594540172 1106 5 0.14677049795075447 1106 6 0.10216767846806363 1106 7 0.09588513052073466 1106 8 0.03148352843795976 1106 9 0.11525654452493228 1106 10-11 0.1536142800616318 1106 12-13 0.05547081495789996 1106 14-15 0.146201257041227 1106 16-17 0.25602379049595214 1106 18-19 0.20130018481950884 1106 20-21 0.26756955782365566 1106 22-23 0.3228360931308245 1106 24-25 0.2880598950014246 1106 26-27 0.21124136333122578 1106 28-29 0.27212844257560675 1106 30-31 0.07923889264501582 1106 32-33 0.2070426893240196 1106 34-35 0.24567421782752774 1106 36-37 0.21620044124696847 1106 38-39 0.1624960207757482 1106 40-41 0.09280564107121592 1106 42-43 0.06799381607450528 1106 44-45 0.12018707963783015 1106 46-47 0.08394514602346703 1106 48-49 0.041442770706233034 1106 50-51 0.035629891034204775 1106 52-53 0.004144351782578326 1106 54-55 0.015663334189348177 1106 56-57 -0.09527392696973891 1106 58-59 0.03665773865350985 1106 60-61 -0.11152600430663995 1106 62-63 -0.16378249366160702 1106 64-65 -0.19296761494846493 1106 66-67 -0.24970831148416295 1106 68-69 -0.5459785638999541 1106 70-71 -0.7420181366678662 1106 72-73 -0.6630030049879032 1106 74-75 -0.5975238835493961 1106 76-77 -0.5922284927285766 1106 78-79 -0.33254130324723974 1106 80-81 -0.34992329033728353 1106 82-83 -0.3509304599503338 1106 84-85 -0.4838035350159089 1106 86-87 -0.5994228651980862 1106 88-89 -0.8419277095457112 1106 90-91 -1.0141971136556194 1106 92-93 -1.0487768073059733 1106 94-95 -0.8699151589951875 1106 96-97 -1.2171413320017486 1106 98-99 -1.2173676670394897 1106 100-101 -1.6816398628209868 1107 1 -0.0781561771300403 1107 2 -0.056054637395526186 1107 3 -0.05006523748680536 1107 4 -0.04561423328323144 1107 5 -0.01298714112623145 1107 6 -0.05489312028681326 1107 7 -0.06958520961006087 1107 8 -0.03631174816820959 1107 9 -0.0608390670096739 1107 10-11 -0.05185722092509337 1107 12-13 -0.07725586366128567 1107 14-15 -0.09402132633617555 1107 16-17 -0.17300029511502402 1107 18-19 -0.07546420871926074 1107 20-21 -0.14647110223042858 1107 22-23 -0.12113723727888015 1107 24-25 -0.10052405423048327 1107 26-27 -0.1018274023349548 1107 28-29 -0.16936420318705814 1107 30-31 -0.12848649613424357 1107 32-33 -0.11878366812502605 1107 34-35 0.037350128347029 1107 36-37 -0.1463749606456588 1107 38-39 -0.11736452603038572 1107 40-41 -0.08351794967116888 1107 42-43 0.014272842847283584 1107 44-45 -0.06530340349321762 1107 46-47 0.04837318800345969 1107 48-49 -0.08523617093710811 1107 50-51 -0.11829046994898818 1107 52-53 -0.2057884905516829 1107 54-55 -0.1341155657491626 1107 56-57 0.02023263616577964 1107 58-59 -0.04205614045409334 1107 60-61 -0.05803999692015793 1107 62-63 -0.04449820434190599 1107 64-65 -0.29113722459990754 1107 66-67 -0.23204524347090683 1107 68-69 -0.25605006402678754 1107 70-71 -0.4863439158386669 1107 72-73 -0.5900542804246207 1107 74-75 -0.5615857184756798 1107 76-77 -0.5877786524537534 1107 78-79 -0.5946828889764504 1107 80-81 -0.6251679299775041 1107 82-83 -0.435220854512373 1107 84-85 -0.5589385382620051 1107 86-87 -0.8720093597141165 1107 88-89 -1.1125789067321428 1107 90-91 -1.3006506772683721 1107 92-93 -1.250240453167521 1107 94-95 -1.603389192367647 1107 96-97 -1.81652919546789 1107 98-99 -1.773687345267419 1107 100-101 -2.2825396336594235 1108 1 -0.005856288193903936 1108 2 -0.023199621836951678 1108 3 -0.07506423970120579 1108 4 0.03300198259077547 1108 5 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-0.17091192593818505 2316 52-53 0.08499271568874178 2316 54-55 -0.03948952993427213 2316 56-57 0.05794939536249899 2316 58-59 -0.15537132471794024 2316 60-61 0.18086430449167779 2316 62-63 0.2317116604049616 2316 64-65 0.45197878666844105 2316 66-67 0.4950518389494789 2316 68-69 0.69876418944515 2316 70-71 0.7183251999913836 2316 72-73 0.849373488475397 2316 74-75 1.0366092041779815 2316 76-77 0.8658322435776142 2316 78-79 0.9407398388204555 2316 80-81 1.0271161345354365 2316 82-83 1.2773326454299685 2316 84-85 1.371238806427538 2316 86-87 1.4987622091232993 2316 88-89 1.7122580811263752 2316 90-91 1.738030481196894 2316 92-93 1.7037951475639304 2316 94-95 2.161911791141687 2316 96-97 2.5044970643960305 2316 98-99 2.848652237276525 2316 100-101 3.080151013941112 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 7.0 4 16.0 5 23.0 6 68.0 7 177.0 8 433.0 9 887.0 10 1559.0 11 2600.0 12 4068.0 13 6209.0 14 8250.0 15 11165.0 16 14491.0 17 19715.0 18 20442.0 19 17102.0 20 16444.0 21 17493.0 22 18983.0 23 21108.0 24 23457.0 25 27145.0 26 31305.0 27 36795.0 28 44727.0 29 53677.0 30 64800.0 31 78494.0 32 97641.0 33 125711.0 34 169611.0 35 239912.0 36 343197.0 37 439175.0 38 251286.0 39 13778.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.82714067288611 18.98351879582832 14.757962554624903 23.431377976660666 2 15.75596076719136 19.78443561201111 40.102538249287676 24.35706537150985 3 19.620263989622615 23.27845477042013 29.69406153876622 27.407219701191043 4 13.05675682608948 16.662818700485563 36.64974011601505 33.630684357409905 5 13.768022668642132 37.21927482668174 34.504735535508864 14.507966969167263 6 32.92191534396773 36.07451970355221 16.795692352837133 14.20787259964292 7 28.937502756582717 32.39765539700642 20.221840776181686 18.443001070229176 8 26.22691558871156 35.25162987172551 19.36043680472233 19.161017734840595 9 25.804044548176964 16.947740592289488 20.131424862980964 37.11678999655258 10-11 25.129098082138512 26.36008666246016 27.001144038085396 21.50967121731593 12-13 25.972264321328282 23.554984282875388 27.461008726835274 23.01174266896106 14-15 22.655802055308076 25.16760022934768 25.781856870124226 26.394740845220017 16-17 22.265408668879047 28.370727540353585 26.663532698843618 22.700331091923747 18-19 22.219136849817772 27.033210408496306 28.740716504482087 22.00693623720383 20-21 22.65649963950649 26.06381590257944 29.17587402799518 22.10381042991889 22-23 22.540385624544882 25.967346758753784 28.977827623794195 22.514439992907143 24-25 22.55177319551902 26.193443970946205 28.93997129005929 22.31481154347549 26-27 22.035035866152214 26.501115113119443 28.978829168361266 22.48501985236708 28-29 22.204261390487936 26.99134939531283 28.730562019254574 22.073827194944663 30-31 22.28973521036493 26.421737116861106 28.651293478441875 22.63723419433209 32-33 22.070776442716635 26.33717889749293 28.843463906093465 22.74858075369697 34-35 22.813877150160256 26.47242965352134 28.538658437729126 22.175034758589277 36-37 22.752540407882943 26.10902696814243 28.624670416998864 22.513762206975763 38-39 22.22196218463379 26.503498580719842 28.605122370438647 22.66941686420772 40-41 22.766041618284824 26.381065282630967 28.4704182171359 22.38247488194831 42-43 22.36863157747873 26.294590124940903 28.35934084060398 22.97743745697639 44-45 22.46353884913909 26.487744570769696 28.551738694860468 22.49697788523075 46-47 22.8686514097977 26.81923535633115 27.86291320686784 22.449200027003307 48-49 22.595931847005907 26.671623291697085 27.915355126281423 22.817089735015585 50-51 22.646751914659294 26.978304991222483 27.945778118031633 22.42916497608659 52-53 22.925148466901597 26.826302108006082 27.67444649959278 22.574102925499535 54-55 22.53092087496624 26.861891259339277 27.876699072823836 22.730488792870645 56-57 22.650753553960513 26.99574091895683 27.737659152883094 22.615846374199567 58-59 22.766850622801694 26.669430622788187 27.639268613995295 22.924450140414823 60-61 22.427824023680863 26.992880123733137 27.86371982086329 22.715576031722705 62-63 22.418154445398 26.965250989583016 28.030311726246467 22.586282838772522 64-65 22.09148020173684 27.120136411842765 27.834561027914372 22.953822358506024 66-67 22.189529090623065 27.02172847941604 27.636941498274158 23.151800931686733 68-69 22.465395491830492 26.72647756686071 27.883829065108223 22.924297876200576 70-71 22.469481955648543 26.863193592172834 27.482793334790244 23.18453111738838 72-73 22.48079634789677 26.81253805961987 27.54013222356395 23.166533368919406 74-75 22.337794686527445 27.4198347315761 27.260647515887214 22.98172306600924 76-77 22.584512792719146 26.86385493032179 27.485304960886754 23.066327316072307 78-79 22.53041791888675 27.096707103846786 27.53697237943124 22.83590259783523 80-81 22.667376032090683 27.127758693327287 27.264545592032064 22.94031968254997 82-83 22.656471235476825 27.097872267625796 27.357125073890536 22.888531423006846 84-85 22.509550495919054 27.261649489316024 27.271800372859285 22.956999641905636 86-87 22.684157771088444 27.277092075002653 27.07520411078019 22.96354604312871 88-89 22.484148443181418 27.444716211498736 26.792197687161583 23.27893765815826 90-91 22.505675595008988 27.241865655751248 26.96615531165015 23.286303437589613 92-93 22.625660412521913 27.037811395209445 27.11143502607663 23.22509316619201 94-95 22.393704997962086 26.974001810828103 27.14772517814016 23.48456801306965 96-97 22.458618147248906 26.785719108436794 27.16566604820073 23.589996696113566 98-99 22.267462690866665 27.022047901884495 26.85082828166438 23.85966112558446 100-101 22.835272443954537 26.436396436459454 26.755810692872156 23.97252042671386 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 552.0 1 366.0 2 281.0 3 371.5 4 300.5 5 284.5 6 538.5 7 1226.5 8 2060.0 9 2448.5 10 2089.5 11 1633.0 12 1622.0 13 1724.0 14 1865.0 15 2000.0 16 2143.5 17 2373.5 18 2543.5 19 2791.0 20 3231.0 21 3891.0 22 4821.0 23 6004.0 24 7637.5 25 9841.0 26 12568.5 27 15999.0 28 18974.0 29 21753.0 30 24981.0 31 28710.5 32 34364.0 33 37750.0 34 39106.0 35 42153.0 36 46709.5 37 51282.5 38 54734.0 39 57782.5 40 61024.5 41 65848.5 42 69170.0 43 71594.0 44 75388.0 45 79172.0 46 81483.0 47 85349.5 48 89263.5 49 87809.5 50 84519.0 51 81412.0 52 79652.0 53 80292.0 54 81531.0 55 77402.5 56 70052.5 57 64012.5 58 58344.0 59 50903.5 60 40466.0 61 32786.5 62 27447.0 63 20712.5 64 14968.5 65 11224.0 66 8226.0 67 6032.0 68 4390.0 69 3115.5 70 2176.5 71 1500.0 72 1065.0 73 824.5 74 578.0 75 378.5 76 261.0 77 147.0 78 81.5 79 53.0 80 31.0 81 13.5 82 8.0 83 7.5 84 4.5 85 1.0 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.009496146184844512 2 0.01548186866154745 3 0.009001086431132238 4 4.5005432155661185E-5 5 0.0013501629646698356 6 0.0020252444470047534 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 6.525787662570872E-4 14-15 0.0 16-17 0.0028578449418844853 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.00339791012775242 26-27 0.019374838543012144 28-29 0.006480782230415211 30-31 0.0034654182759859117 32-33 0.0 34-35 0.009046091863287899 36-37 7.875950627240708E-4 38-39 0.002385287904250043 40-41 0.0020702498791604147 42-43 0.0028578449418844853 44-45 0.0 46-47 1.8002172862264474E-4 48-49 5.400651858679343E-4 50-51 0.0037579535849977096 52-53 0.007313382725294943 54-55 0.006930836551971823 56-57 0.015661890390170092 58-59 0.022525218793908425 60-61 0.00339791012775242 62-63 0.01968987656810177 64-65 0.02007242274142489 66-67 0.012804045448285608 68-69 0.03040116942114913 70-71 0.016134447427804538 72-73 0.004995602969278392 74-75 0.016021933847415383 76-77 0.0205224770629815 78-79 0.025473074600104235 80-81 0.011251358038915298 82-83 0.03433914473476949 84-85 0.02126506669354991 86-87 0.03026615312468215 88-89 0.021805131879417846 90-91 0.027048264725552378 92-93 0.023425327437021647 94-95 0.01433423014157809 96-97 0.01476178174705687 98-99 0.011071336310292652 100-101 0.010396254827957734 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2221954.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.9392385598093 #Duplication Level Percentage of deduplicated Percentage of total 1 73.35988118996968 38.102563698451014 2 14.437843382108676 14.997811834250127 3 5.205864691576621 8.111659443776587 4 2.391325429189849 4.968144877633197 5 1.2416611188593931 3.224546653143887 6 0.7515179471801697 2.34199619643414 7 0.5199208612335438 1.8903005550681375 8 0.3461808831569808 1.4384297180108716 9 0.26451602691841175 1.2364884922507493 >10 1.3432226768423916 12.71528220614025 >50 0.08992122673778834 3.177142026813791 >100 0.04207608355720251 4.087260993679816 >500 0.0039011674302116377 1.4255700577435466 >1k 0.0019939300198858778 1.6844780751795536 >5k 1.733852191205111E-4 0.598325171424376 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7009 0.31544307397902926 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6279 0.2825891085053966 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3763 0.16935544120175305 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2420 0.10891314581670007 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2266 0.10198230926472825 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.500543215566119E-5 0.0 5 0.0 0.0 0.0 4.500543215566119E-5 0.0 6 0.0 0.0 0.0 4.500543215566119E-5 0.0 7 0.0 0.0 0.0 4.500543215566119E-5 0.0 8 0.0 0.0 0.0 4.500543215566119E-5 0.0 9 0.0 0.0 0.0 4.500543215566119E-5 0.0 10-11 0.0 0.0 0.0 4.500543215566119E-5 4.500543215566119E-5 12-13 0.0 0.0 0.0 1.1251358038915298E-4 3.150380250896283E-4 14-15 0.0 0.0 0.0 1.3501629646698357E-4 3.375407411674589E-4 16-17 0.0 0.0 0.0 1.5751901254481415E-4 4.275516054787813E-4 18-19 0.0 0.0 0.0 2.2502716077830594E-4 4.950597537122731E-4 20-21 0.0 0.0 0.0 2.7003259293396714E-4 5.400651858679343E-4 22-23 0.0 0.0 0.0 3.8254617332312006E-4 5.400651858679343E-4 24-25 0.0 0.0 0.0 7.875950627240708E-4 5.400651858679343E-4 26-27 0.0 0.0 0.0 0.0012601521003585132 6.07573334101426E-4 28-29 0.0 0.0 0.0 0.004838083956733578 6.300760501792566E-4 30-31 0.0 0.0 0.0 0.014919300759601684 6.300760501792566E-4 32-33 0.0 0.0 0.0 0.03798458473937804 6.300760501792566E-4 34-35 0.0 0.0 0.0 0.06930836551971822 6.300760501792566E-4 36-37 0.0 0.0 0.0 0.11867932459447855 6.300760501792566E-4 38-39 0.0 0.0 0.0 0.1901479508576685 6.300760501792566E-4 40-41 0.0 0.0 0.0 0.26557705515055663 6.300760501792566E-4 42-43 0.0 0.0 0.0 0.34399902068179633 6.525787662570872E-4 44-45 0.0 0.0 0.0 0.44312348500464005 6.975841984127485E-4 46-47 0.0 0.0 0.0 0.5607676846595384 7.200869144905791E-4 48-49 0.0 0.0 0.0 0.6791094685128496 7.650923466462402E-4 50-51 0.0 4.500543215566119E-5 0.0 0.7961235921175686 7.650923466462402E-4 52-53 0.0 4.500543215566119E-5 0.0 0.9130927102901321 7.650923466462402E-4 54-55 0.0 4.500543215566119E-5 0.0 1.0396479855118512 8.100977788019014E-4 56-57 0.0 4.500543215566119E-5 0.0 1.1844529634726912 8.100977788019014E-4 58-59 0.0 4.500543215566119E-5 0.0 1.3360987671211917 8.100977788019014E-4 60-61 0.0 4.500543215566119E-5 0.0 1.4943153638644184 8.100977788019014E-4 62-63 0.0 4.500543215566119E-5 0.0 1.6525319606076454 8.551032109575626E-4 64-65 0.0 4.500543215566119E-5 0.0 1.815969187480929 8.551032109575626E-4 66-67 0.0 4.500543215566119E-5 0.0 2.003551828705725 9.001086431132237E-4 68-69 0.0 4.500543215566119E-5 0.0 2.212152006747214 9.001086431132237E-4 70-71 0.0 4.500543215566119E-5 0.0 2.425522760597204 9.226113591910543E-4 72-73 0.0 4.500543215566119E-5 0.0 2.639681109509918 9.901195074245462E-4 74-75 0.0 4.500543215566119E-5 0.0 2.8610403275675376 0.0010351249395802074 76-77 0.0 4.500543215566119E-5 0.0 3.092053210822546 0.0010351249395802074 78-79 0.0 4.500543215566119E-5 0.0 3.353782301523794 0.0010351249395802074 80-81 0.0 4.500543215566119E-5 0.0 3.6212495848248887 0.0010351249395802074 82-83 0.0 4.500543215566119E-5 0.0 3.9005757994989994 0.0011251358038915297 84-85 0.0 4.500543215566119E-5 0.0 4.187980489245052 0.0011251358038915297 86-87 0.0 4.500543215566119E-5 0.0 4.481325896035651 0.0011701412360471909 88-89 4.500543215566119E-5 4.500543215566119E-5 0.0 4.79199839420618 0.001215146668202852 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6165 0.0 31.597046 1 GTATCAA 8775 0.0 30.861954 1 TCAACGC 10220 0.0 26.022385 4 ATCAACG 10265 0.0 25.95866 3 TATCAAC 10360 0.0 25.726414 2 CAACGCA 10400 0.0 25.571999 5 AACGCAG 10905 0.0 24.430782 6 TAAGGTG 1190 0.0 22.74772 5 GTAAGGT 1085 0.0 22.32289 4 ACGCAGA 12060 0.0 22.051641 7 CGCAGAG 12040 0.0 22.009384 8 GTACATG 6005 0.0 21.83694 1 TACATGG 6085 0.0 21.31465 2 GAGTACT 7330 0.0 21.024733 12-13 ACATGGG 6030 0.0 20.874062 3 GTACTTT 7380 0.0 20.560064 14-15 GTGTAAG 1005 0.0 20.328161 1 GCAGAGT 12885 0.0 20.271154 9 ACGACCG 60 0.004769679 19.788746 46-47 AGAGTAC 9990 0.0 19.704885 10-11 >>END_MODULE