FastQCFastQC Report
Thu 26 May 2016
SRR938091_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938091_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2508558
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83160.3315051914287013No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70530.28115754150392375No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43100.17181185366254237No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT30140.12014870694638115No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA29840.11895280077239594No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA26790.10679442133687958No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA25390.10121352585828194No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73650.032.120151
GTATCAA105150.028.8026961
TAAGGTG14900.023.922545
ATCAACG124000.023.878043
TCAACGC124700.023.7058894
CAACGCA124850.023.6393415
TATCAAC126100.023.6311452
GTACATG64900.023.516571
TACATGG61450.023.51172
AACGCAG128800.023.0578356
GTGTAAG11500.022.8104341
GGACCGA5300.022.4139546
GAGTACT84300.021.38539512-13
GTAAGGT15600.021.325824
AGAGTAC109400.020.86538910-11
ACATGGG67000.020.7128733
TATACCG1856.5260756E-620.5518345
GTATAGA8650.020.4011331
ACGCAGA143950.020.3629747
GTGTAGC15300.020.262391
Produced by FastQC (version 0.11.5)