FastQCFastQC Report
Thu 26 May 2016
SRR938091_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938091_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2508558
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82660.32951201447205924No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69980.27896504685161755No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43880.17492120971490394No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT30990.1235371077726726No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA29760.11863389245933322No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA28980.11552453640697165No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA27030.10775114627606776No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA26140.10420329129324497No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79850.030.1955051
GTATCAA111800.028.074461
ATCAACG131400.023.5215843
TCAACGC132400.023.343934
CAACGCA133150.023.1767275
TATCAAC134300.023.1231212
AACGCAG138300.022.390466
GAGTACT87700.021.71612712-13
TAAGGTG14500.021.3154245
GTACATG69550.021.2653481
TACATGG66500.020.810412
GTACTTT92700.020.31426214-15
ACGCAGA151450.020.2895577
AGAGTAC117600.020.01123610-11
CGCAGAG153150.020.0023168
GTACGGG1456.0998445E-419.6785241
GTAAGGT14900.019.1475684
AGTACTT93400.019.01788912-13
ACATGGG69700.018.4877953
GCAGAGT162850.018.4609289
Produced by FastQC (version 0.11.5)