##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938091_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2508558 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.43445078806231 33.0 31.0 34.0 31.0 34.0 2 32.664198316323564 34.0 31.0 34.0 31.0 34.0 3 32.7381073110528 34.0 31.0 34.0 31.0 34.0 4 36.21014662606964 37.0 35.0 37.0 35.0 37.0 5 36.129706389088874 37.0 35.0 37.0 35.0 37.0 6 36.17826735518972 37.0 37.0 37.0 35.0 37.0 7 36.143638297380406 37.0 36.0 37.0 35.0 37.0 8 36.13770779866361 37.0 36.0 37.0 35.0 37.0 9 37.82133959031444 39.0 38.0 39.0 35.0 39.0 10-11 37.790621544329454 39.0 38.0 39.0 35.0 39.0 12-13 37.71469625179087 39.0 37.5 39.0 35.0 39.0 14-15 38.95367717230377 40.0 38.0 41.0 35.5 41.0 16-17 38.86571309094707 40.0 38.0 41.0 35.0 41.0 18-19 39.106825913532795 40.0 38.5 41.0 36.0 41.0 20-21 39.07201886502126 40.0 39.0 41.0 36.0 41.0 22-23 38.96853690446862 40.0 38.5 41.0 35.0 41.0 24-25 38.86359454315985 40.0 38.0 41.0 35.0 41.0 26-27 38.702852594996806 40.0 38.0 41.0 34.5 41.0 28-29 38.58241408012093 40.0 38.0 41.0 34.0 41.0 30-31 38.34266658375051 40.0 38.0 41.0 34.0 41.0 32-33 38.12120210096796 40.0 38.0 41.0 34.0 41.0 34-35 38.033585430354805 40.0 38.0 41.0 33.0 41.0 36-37 37.982864458386054 40.0 38.0 41.0 33.0 41.0 38-39 37.79150731216899 40.0 37.0 41.0 33.0 41.0 40-41 37.57973923664511 40.0 37.0 41.0 33.0 41.0 42-43 37.32770300706621 40.0 37.0 41.0 32.0 41.0 44-45 37.06444299872676 40.0 36.0 41.0 31.0 41.0 46-47 36.723631265452106 39.0 36.0 41.0 30.5 41.0 48-49 36.320759775137745 39.0 35.5 41.0 30.0 41.0 50-51 35.93368241834552 39.0 35.0 40.0 29.0 41.0 52-53 35.397167217182144 38.0 35.0 40.0 27.5 41.0 54-55 35.016180411216325 38.0 34.0 40.0 26.5 41.0 56-57 34.63338419920927 38.0 34.0 40.0 26.0 41.0 58-59 34.12259034871826 37.5 33.0 40.0 24.5 41.0 60-61 33.62840205408844 37.0 33.0 40.0 23.0 41.0 62-63 33.061218636364 36.0 32.0 39.0 21.5 40.5 64-65 32.48589687780789 36.0 31.0 39.0 19.5 40.0 66-67 31.907782479017825 35.0 31.0 38.5 17.0 40.0 68-69 31.274277493284988 35.0 30.0 38.0 10.5 40.0 70-71 30.639226998139968 34.5 29.0 37.5 7.5 39.5 72-73 29.953412478403926 34.0 29.0 36.5 4.5 39.0 74-75 29.29623871562866 34.0 28.0 36.0 2.0 39.0 76-77 28.010535534757416 32.0 26.5 34.5 2.0 37.0 78-79 28.66044815387964 33.5 28.0 35.0 2.0 37.0 80-81 28.372142681173806 34.0 27.0 35.0 2.0 37.0 82-83 27.803131520180116 33.0 26.0 35.0 2.0 36.5 84-85 27.13036194498991 33.0 25.5 35.0 2.0 36.0 86-87 26.409599857766892 32.0 24.5 35.0 2.0 35.5 88-89 25.583641279173136 32.0 21.0 34.0 2.0 35.0 90-91 24.91569937788961 31.0 19.0 34.0 2.0 35.0 92-93 23.962607601658007 31.0 9.5 34.0 2.0 35.0 94-95 23.036834906747224 30.5 2.0 34.0 2.0 35.0 96-97 21.973370358588483 30.0 2.0 34.0 2.0 35.0 98-99 20.545768525184588 29.0 2.0 34.0 2.0 35.0 100-101 16.71396535380087 20.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1145133626951278 1101 2 0.12425991634183475 1101 3 0.1727398824483828 1101 4 0.025039866564959823 1101 5 -0.06133822915357712 1101 6 0.006760941489801553 1101 7 0.04351676585399389 1101 8 0.09652406095437982 1101 9 0.14563100853149535 1101 10-11 0.1529147972763809 1101 12-13 0.28934296456120734 1101 14-15 0.3547995173612932 1101 16-17 0.3991778834106299 1101 18-19 0.16733549516776947 1101 20-21 0.25074721339256456 1101 22-23 0.25804988356235725 1101 24-25 0.23668759674850293 1101 26-27 0.20375606993439277 1101 28-29 0.29770731208372325 1101 30-31 0.33888310161626123 1101 32-33 0.4139743111876655 1101 34-35 0.35537251608549525 1101 36-37 0.41629833955204987 1101 38-39 0.3718336213266724 1101 40-41 0.47920524663545905 1101 42-43 0.5608900141785327 1101 44-45 0.5612277353662094 1101 46-47 0.6156427911207274 1101 48-49 0.8300607908793083 1101 50-51 0.7396284823330177 1101 52-53 0.9363798275828898 1101 54-55 1.011136260407632 1101 56-57 1.0792804411005932 1101 58-59 1.2116195387163415 1101 60-61 1.2125296407026696 1101 62-63 1.1565126087054622 1101 64-65 1.471138503518283 1101 66-67 1.6167094459343225 1101 68-69 1.8597832269538266 1101 70-71 1.8693390672234393 1101 72-73 1.9715511060324538 1101 74-75 2.003056913612234 1101 76-77 1.9715740438794853 1101 78-79 2.167151336952333 1101 80-81 2.3696960554970268 1101 82-83 2.2258312076436972 1101 84-85 2.349948474680115 1101 86-87 2.619640390950284 1101 88-89 2.9533037420782406 1101 90-91 3.0667266149841517 1101 92-93 3.378919795069443 1101 94-95 3.6688302947079627 1101 96-97 3.855856595493883 1101 98-99 4.372541256523579 1101 100-101 4.43586759849509 1102 1 0.18922543439659734 1102 2 0.167089662772689 1102 3 0.2903716549145372 1102 4 0.17417904824846886 1102 5 0.043503865902003724 1102 6 0.11086277404884726 1102 7 0.10431100945713467 1102 8 0.13050255612683515 1102 9 0.10791356041048061 1102 10-11 0.22717093022676238 1102 12-13 0.29689955391818756 1102 14-15 0.2985492435641177 1102 16-17 0.3734336123108122 1102 18-19 0.2748904683239459 1102 20-21 0.3282509529834883 1102 22-23 0.31598791757317457 1102 24-25 0.4328596038955155 1102 26-27 0.37250253183692905 1102 28-29 0.2940095084580676 1102 30-31 0.34715156362682364 1102 32-33 0.3051213799310659 1102 34-35 0.3387471263365427 1102 36-37 0.38538956260349977 1102 38-39 0.5494368635956022 1102 40-41 0.7190140373217133 1102 42-43 0.7798740575981995 1102 44-45 0.7052697174578597 1102 46-47 0.7048343206974081 1102 48-49 0.7591865580962107 1102 50-51 0.9252983741709073 1102 52-53 0.9411092351644825 1102 54-55 0.9845423142704988 1102 56-57 1.0218593786867416 1102 58-59 1.1714042097322874 1102 60-61 1.0859469867053235 1102 62-63 1.2801300658857144 1102 64-65 1.1708979447891537 1102 66-67 1.4295941176733074 1102 68-69 1.6650407068799744 1102 70-71 1.6532287776810684 1102 72-73 1.9590287397273478 1102 74-75 2.1011309551732644 1102 76-77 1.9607096656680874 1102 78-79 1.9781704663900257 1102 80-81 2.1947224357218715 1102 82-83 2.313450662958509 1102 84-85 2.487628367398404 1102 86-87 2.4628853946569187 1102 88-89 2.563900894247375 1102 90-91 2.6638573056590573 1102 92-93 2.77346971079308 1102 94-95 3.3064040710844615 1102 96-97 3.5450031266378588 1102 98-99 3.776406762247696 1102 100-101 3.6936692761965766 1103 1 0.22160779150299703 1103 2 0.17011029229934138 1103 3 0.0904902931400926 1103 4 0.11371342815512264 1103 5 0.0337902070288294 1103 6 0.04338574170636633 1103 7 0.0924969278068275 1103 8 0.12174034622473329 1103 9 0.15666684161452338 1103 10-11 0.15588265516530697 1103 12-13 0.2282453886620388 1103 14-15 0.36649511477093455 1103 16-17 0.287458923854345 1103 18-19 0.23807520995180198 1103 20-21 0.2930985207457084 1103 22-23 0.28092006542863146 1103 24-25 0.22122191747245523 1103 26-27 0.3147460439888192 1103 28-29 0.37538737675607337 1103 30-31 0.40816577246586405 1103 32-33 0.33597533467218454 1103 34-35 0.44242926879655187 1103 36-37 0.40266856653570215 1103 38-39 0.42656346714886695 1103 40-41 0.5355967096868 1103 42-43 0.47263270670919866 1103 44-45 0.5693612234583085 1103 46-47 0.5835241658038228 1103 48-49 0.8137647665233416 1103 50-51 0.8406280484645805 1103 52-53 0.8225972117393994 1103 54-55 0.9096048655144244 1103 56-57 0.8791836550644803 1103 58-59 1.1026895126718728 1103 60-61 1.1725188743377402 1103 62-63 1.3888207269896213 1103 64-65 1.3406905807097758 1103 66-67 1.5031839373131994 1103 68-69 1.637431524051113 1103 70-71 1.7818193979028827 1103 72-73 1.8002923560432684 1103 74-75 1.880915531833967 1103 76-77 2.127844246264896 1103 78-79 2.0256243178262743 1103 80-81 2.0829865829500207 1103 82-83 2.1826931274682693 1103 84-85 2.5246676653856888 1103 86-87 2.7402443827871323 1103 88-89 2.891767044348576 1103 90-91 3.152206731819607 1103 92-93 2.986022876524224 1103 94-95 3.306978455217031 1103 96-97 3.72223624150174 1103 98-99 4.063027733054511 1103 100-101 3.993347131894925 1104 1 0.1761282577680987 1104 2 0.14717387048504804 1104 3 0.19763011800572627 1104 4 0.12705882635182775 1104 5 0.017485195497037864 1104 6 0.09013980109480002 1104 7 0.10362668673054287 1104 8 0.12583375431989907 1104 9 0.11875847487213775 1104 10-11 0.19232129513885354 1104 12-13 0.2565131076701377 1104 14-15 0.42298102559252015 1104 16-17 0.41662788272874707 1104 18-19 0.3266535798073278 1104 20-21 0.3288049142316112 1104 22-23 0.3199136689575255 1104 24-25 0.3669623267396105 1104 26-27 0.4628703492996422 1104 28-29 0.44338075193649473 1104 30-31 0.36797198253638186 1104 32-33 0.3719690052711755 1104 34-35 0.3086960542509516 1104 36-37 0.3551774145544684 1104 38-39 0.42174004177442725 1104 40-41 0.49823081595451413 1104 42-43 0.5269067582198019 1104 44-45 0.49352655938450596 1104 46-47 0.6077222226319705 1104 48-49 0.7848355541627541 1104 50-51 0.9617092455378469 1104 52-53 0.905049329312881 1104 54-55 0.9512299041378824 1104 56-57 0.9975764268466563 1104 58-59 1.0610272559608873 1104 60-61 1.0562491812202524 1104 62-63 1.232689492911497 1104 64-65 1.3891813872606278 1104 66-67 1.4482973381120736 1104 68-69 1.691938091686655 1104 70-71 1.6743903574820997 1104 72-73 1.9129234964946384 1104 74-75 1.8914104509834857 1104 76-77 1.8689004684506756 1104 78-79 1.89897271673129 1104 80-81 2.118087794825229 1104 82-83 2.1532569155872494 1104 84-85 2.2478037661164443 1104 86-87 2.5540145711765625 1104 88-89 2.647610671778459 1104 90-91 2.8012982227955767 1104 92-93 3.1002256088746165 1104 94-95 3.2376385412605977 1104 96-97 3.5167295928393223 1104 98-99 3.7072253362872125 1104 100-101 3.3713143536150874 1105 1 0.19808028205048345 1105 2 0.18966988628748993 1105 3 0.1902566767146041 1105 4 0.1262173283968835 1105 5 -0.044592994426096766 1105 6 -0.008281135374318183 1105 7 0.11352941990113408 1105 8 0.08235709813751413 1105 9 0.13048223914346835 1105 10-11 0.14522509051607102 1105 12-13 0.2326060769547169 1105 14-15 0.36440557090681835 1105 16-17 0.2909389062781429 1105 18-19 0.3103556467182216 1105 20-21 0.2762779106360469 1105 22-23 0.32676634111951586 1105 24-25 0.21992569131416673 1105 26-27 0.21229735562637586 1105 28-29 0.2719000772322957 1105 30-31 0.3966788732973967 1105 32-33 0.5089100642446809 1105 34-35 0.4610547951633279 1105 36-37 0.40734715059723214 1105 38-39 0.3441282794045861 1105 40-41 0.5464931226775747 1105 42-43 0.6055288717526466 1105 44-45 0.6374764105353279 1105 46-47 0.6279065475918983 1105 48-49 0.8244808111038182 1105 50-51 0.9381242354214123 1105 52-53 0.9033975204982667 1105 54-55 1.1038069547170721 1105 56-57 0.9938265938544788 1105 58-59 1.1517379674178798 1105 60-61 1.0283361773456718 1105 62-63 1.2493943345511767 1105 64-65 1.239270741056636 1105 66-67 1.2039866762591664 1105 68-69 1.6229293541328822 1105 70-71 1.9105045339914604 1105 72-73 1.938757668250279 1105 74-75 1.7647581808213069 1105 76-77 1.8202338367234 1105 78-79 1.8675197007361852 1105 80-81 2.0012055742745183 1105 82-83 2.0412692236020753 1105 84-85 2.103072761271921 1105 86-87 2.467198740385559 1105 88-89 2.60759486047478 1105 90-91 2.7203542392476017 1105 92-93 2.9922807978536454 1105 94-95 3.2627522726618317 1105 96-97 3.6699839016410216 1105 98-99 3.7790746830559705 1105 100-101 3.7657094974337326 1106 1 0.14112855884077646 1106 2 0.10270149318650112 1106 3 0.07683832553369285 1106 4 0.047361662456459896 1106 5 -0.0510292688946663 1106 6 0.01734759572308775 1106 7 0.030558856489655284 1106 8 0.08100976714273145 1106 9 0.11838500138230756 1106 10-11 0.12912739429786058 1106 12-13 0.09198997095159456 1106 14-15 0.06723426499117835 1106 16-17 -0.03678165783435361 1106 18-19 -0.02338426932945481 1106 20-21 -0.019983841707407635 1106 22-23 -0.07663363672873658 1106 24-25 0.012262868957165551 1106 26-27 -0.18383215218422322 1106 28-29 0.06683219894913606 1106 30-31 0.1824733990116556 1106 32-33 0.19697875429125844 1106 34-35 0.1872470989731454 1106 36-37 0.11410672842416858 1106 38-39 0.12507395574792923 1106 40-41 0.2843389282861679 1106 42-43 0.25226001682977284 1106 44-45 0.2745121351358293 1106 46-47 0.31294321714670303 1106 48-49 0.2839441639811682 1106 50-51 0.4234196107533421 1106 52-53 0.4335354641991813 1106 54-55 0.570274220602542 1106 56-57 0.558069992493401 1106 58-59 0.7428629677683816 1106 60-61 0.7443181727414725 1106 62-63 0.7135970048182045 1106 64-65 0.7228595204953265 1106 66-67 1.0524632879390232 1106 68-69 1.0862443772239807 1106 70-71 1.0291770904994486 1106 72-73 1.380255497939789 1106 74-75 1.414734802315234 1106 76-77 1.2576851168981449 1106 78-79 1.3749824213073047 1106 80-81 1.35398863295422 1106 82-83 1.768177253190082 1106 84-85 1.8936767048438519 1106 86-87 1.9373965779263251 1106 88-89 2.0618809432886707 1106 90-91 2.1984632651034595 1106 92-93 2.156065809532386 1106 94-95 2.280014162402207 1106 96-97 2.830640154182163 1106 98-99 2.9922062181720968 1106 100-101 2.7913016010366185 1107 1 0.19156527190651929 1107 2 0.16504383625877495 1107 3 0.15393830230714656 1107 4 0.11310561410203235 1107 5 0.05749878931213459 1107 6 0.10646730965847695 1107 7 0.12147390134929736 1107 8 0.15686064191924487 1107 9 0.12043352865729418 1107 10-11 0.18572150501825746 1107 12-13 0.20842551998931214 1107 14-15 0.4143185233351758 1107 16-17 0.2830448317704253 1107 18-19 0.278506803574885 1107 20-21 0.28134884884479305 1107 22-23 0.39199466022534324 1107 24-25 0.4093767006349296 1107 26-27 0.30968095731003586 1107 28-29 0.38598349828529166 1107 30-31 0.4725435782881675 1107 32-33 0.5580921160365975 1107 34-35 0.5100734589413847 1107 36-37 0.7072458500286487 1107 38-39 0.6806147696492815 1107 40-41 0.6230855995981415 1107 42-43 0.5950709959636882 1107 44-45 0.7487735445009847 1107 46-47 0.9236605797995026 1107 48-49 1.0376063959981465 1107 50-51 1.0673154856108766 1107 52-53 0.9992584849464308 1107 54-55 0.9200595776161506 1107 56-57 1.0371124241506493 1107 58-59 1.2110074908950068 1107 60-61 1.146167788963325 1107 62-63 1.2669326473022622 1107 64-65 1.511597886966996 1107 66-67 1.6387461899608162 1107 68-69 1.816328965060837 1107 70-71 1.9531138683834577 1107 72-73 1.9897406383523553 1107 74-75 1.940897412438762 1107 76-77 1.929915688973356 1107 78-79 2.015830824887434 1107 80-81 2.1577297271945746 1107 82-83 2.472367281440043 1107 84-85 2.4537166491001408 1107 86-87 2.688576810515869 1107 88-89 2.7305610273363534 1107 90-91 2.8430410821322987 1107 92-93 3.128949830749562 1107 94-95 3.0696896929667226 1107 96-97 3.331480090876326 1107 98-99 3.636549406582592 1107 100-101 3.493282406727662 1108 1 0.24749233356154576 1108 2 0.19625605573071425 1108 3 0.23351099536258602 1108 4 0.1877627328794702 1108 5 0.13394316729002753 1108 6 0.1527809084822067 1108 7 0.18196572433271285 1108 8 0.20993542657460296 1108 9 0.17768172951797112 1108 10-11 0.23506435968526063 1108 12-13 0.3084157713027693 1108 14-15 0.36419280371439555 1108 16-17 0.4079496161043181 1108 18-19 0.39204301208357606 1108 20-21 0.32515958595730154 1108 22-23 0.2916443364571393 1108 24-25 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2316 54-55 0.05460112192139377 2316 56-57 0.23288955239247144 2316 58-59 0.53204478051628 2316 60-61 0.557973875310438 2316 62-63 0.622512645735469 2316 64-65 0.8074640999700833 2316 66-67 0.8419324919745641 2316 68-69 1.2441398724656132 2316 70-71 1.4052409986367032 2316 72-73 1.4432982592016863 2316 74-75 1.8350916585053767 2316 76-77 1.8092092780464704 2316 78-79 1.7267608316839187 2316 80-81 1.841460827780427 2316 82-83 2.0587821464737175 2316 84-85 2.3452732875292845 2316 86-87 2.3014463561253606 2316 88-89 2.685227576140825 2316 90-91 2.967942363724834 2316 92-93 3.1338256731642886 2316 94-95 3.694761151331374 2316 96-97 4.114748723512577 2316 98-99 4.401351345168628 2316 100-101 4.7993909853308026 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 6.0 4 10.0 5 36.0 6 78.0 7 225.0 8 612.0 9 1213.0 10 2056.0 11 3499.0 12 5399.0 13 7882.0 14 10825.0 15 14634.0 16 18897.0 17 25303.0 18 26932.0 19 23221.0 20 22964.0 21 24429.0 22 26942.0 23 29699.0 24 33410.0 25 37264.0 26 42363.0 27 49437.0 28 59026.0 29 70278.0 30 83568.0 31 100646.0 32 121323.0 33 150733.0 34 193728.0 35 262082.0 36 361477.0 37 443814.0 38 242564.0 39 11976.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.75081540869296 18.859998453294683 14.899077477977388 23.49010866003497 2 15.542365907608497 19.708946190808057 40.32531747917627 24.42337042240718 3 19.442522073163147 23.276403680960073 30.068495771934835 27.212578473941946 4 12.968233884335078 16.620185112986853 36.79206321491301 33.61951778776507 5 13.634974026264285 37.12675725811859 34.69305653557939 14.545212180037739 6 32.80931340579999 36.204812649189414 16.734687206504457 14.251186738506135 7 28.61325111877023 32.59832939880202 20.350894816862915 18.43752466556484 8 26.131260636120214 35.56794340637284 19.15376645720976 19.147029500297183 9 25.65023411856533 17.001121759991197 20.275672318519245 37.07297180292423 10-11 25.04347118942436 26.413521234111393 27.093234439865455 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22.486390985671147 26.261216935192767 28.981086405057603 22.27130567407848 38-39 21.969970748139993 26.71156270711762 28.89031299988539 22.428153544857004 40-41 22.467130010034722 26.51976983531815 28.872567018739055 22.140533135908075 42-43 22.16484416532136 26.534262819318123 28.679106279013634 22.621786736346888 44-45 22.196837070312508 26.720180126057148 28.830773235669376 22.25220956796097 46-47 22.688158433681508 26.916106168584935 28.16491150790843 22.23082388982513 48-49 22.35852230644059 26.99853860265539 28.13921384317205 22.503725247731964 50-51 22.315031185246664 27.243340596470162 28.241483752817352 22.20014446546582 52-53 22.719646107444994 27.010118960773482 27.998236437028762 22.27199849475276 54-55 22.327109837603913 27.087215045456393 28.14547241881591 22.44020269812378 56-57 22.417110713450004 27.17709822818457 27.98874014024796 22.417050918117468 58-59 22.445982911298046 26.876895012991525 27.956499311556684 22.72062276415375 60-61 22.075351656210458 27.21017413191164 28.251389842291868 22.463084369586035 62-63 22.134808124827092 27.157155624864966 28.310587197904134 22.39744905240381 64-65 21.882790830429276 27.33367137614518 28.088347967238548 22.695189826186997 66-67 21.946769376485914 27.227377024870087 27.916319189206856 22.909534409437143 68-69 22.375208936856723 26.90648279293117 28.08226959741012 22.63603867280199 70-71 22.319157061546914 26.977530517532383 27.72531071635577 22.97800170456493 72-73 22.279601380395185 27.012920176818785 27.852944940059167 22.854533502726866 74-75 22.187647245947673 27.552522205984474 27.50191544305533 22.757915105012522 76-77 22.245309855303326 27.024928265561332 27.836410399918996 22.89335147921635 78-79 22.323023824842796 27.220438993238343 27.770874741584606 22.685662440334248 80-81 22.501072830276755 27.239644073482243 27.529353094803916 22.729930001437086 82-83 22.44810501559994 27.24439092869514 27.73308867219041 22.574415383514516 84-85 22.36852333852512 27.384012989700008 27.592289099758453 22.655174572016413 86-87 22.492100291072855 27.360509567851587 27.390368304930657 22.757021836144897 88-89 22.359379372531947 27.509529379029708 26.99730283294227 23.133788415496074 90-91 22.31296302792758 27.36050561134835 27.29969208543154 23.026839275292527 92-93 22.53731027945138 27.09173955714797 27.43163602356414 22.93931413983651 94-95 22.269969893056068 26.899403342359108 27.560779451936025 23.269847312648803 96-97 22.377599379403804 26.707202611223867 27.59440642234146 23.32079158703086 98-99 22.237317215707193 26.897982825192802 27.18115347542293 23.683546483677077 100-101 22.602826006016205 26.262996510345783 27.416049379763198 23.718128103874818 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 445.0 1 322.5 2 294.5 3 392.5 4 335.0 5 328.5 6 608.5 7 1326.0 8 2174.5 9 2575.5 10 2254.5 11 1876.5 12 1912.0 13 2011.5 14 2155.5 15 2326.0 16 2568.5 17 2810.5 18 3068.0 19 3452.0 20 4042.0 21 4901.5 22 6052.5 23 7759.0 24 9889.0 25 12506.5 26 16053.5 27 20396.5 28 23996.0 29 27119.5 30 30421.5 31 35344.5 32 42588.5 33 46384.5 34 47663.0 35 50608.0 36 54860.5 37 59464.5 38 63729.0 39 66915.5 40 70670.0 41 76113.0 42 78621.0 43 80598.5 44 84084.0 45 87809.5 46 90370.5 47 94807.0 48 99423.0 49 97314.5 50 93198.5 51 89961.0 52 87751.0 53 87553.5 54 87032.5 55 82169.5 56 75259.5 57 69072.5 58 63625.5 59 56012.0 60 45021.5 61 36554.0 62 30556.5 63 22580.5 64 15569.0 65 11803.0 66 8742.5 67 6470.5 68 4850.5 69 3544.0 70 2572.5 71 1715.0 72 1120.0 73 788.0 74 551.5 75 372.5 76 236.0 77 139.5 78 86.5 79 53.5 80 33.5 81 17.5 82 10.0 83 4.0 84 1.5 85 3.0 86 2.5 87 1.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.014430601166088247 3 0.0032688102088929178 4 0.0014350874087822566 5 0.11759744044187936 6 7.972707826568093E-5 7 0.0 8 2.790447739298832E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 3.189083130627237E-4 24-25 4.185671608948248E-4 26-27 0.005820076713394707 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.010942541491964707 36-37 7.374754739575486E-4 38-39 0.006796733422149299 40-41 0.010643564948468404 42-43 0.0058400084829611275 44-45 0.0036873773697877425 46-47 1.9931769566420232E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 3.9863539132840464E-5 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 7.972707826568093E-5 68-69 9.965884783210116E-4 70-71 0.0 72-73 3.9863539132840464E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.59113004363463E-4 82-83 0.002172562882739805 84-85 0.00434512576547961 86-87 0.0031691513610608165 88-89 0.0 90-91 0.0027904477392988324 92-93 0.0 94-95 1.9931769566420232E-5 96-97 9.965884783210116E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2508558.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.99217604638417 #Duplication Level Percentage of deduplicated Percentage of total 1 74.38107486725832 40.9037716361955 2 14.436614655603439 15.878017093095082 3 4.951041755026948 8.16805679416322 4 2.2193558529299224 4.881888310955815 5 1.14253232011917 3.141516924333857 6 0.6834466312530351 2.2550530478505064 7 0.4564040220030564 1.7569055228388912 8 0.30999250020638935 1.3637729715526834 9 0.227909564817228 1.1279918619975424 >10 1.0902886549221007 10.569403980603566 >50 0.06168321900135476 2.2821857121224425 >100 0.03377201297806157 3.4642157930126887 >500 0.0031235751467665485 1.1867461279523548 >1k 0.002615086169379449 2.410722880353272 >5k 1.4528256496552496E-4 0.6097513429726312 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8266 0.32951201447205924 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6998 0.27896504685161755 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4388 0.17492120971490394 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3099 0.1235371077726726 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2976 0.11863389245933322 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2898 0.11552453640697165 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2703 0.10775114627606776 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2614 0.10420329129324497 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.9863539132840464E-5 2 0.0 0.0 0.0 0.0 3.9863539132840464E-5 3 0.0 0.0 0.0 0.0 3.9863539132840464E-5 4 0.0 0.0 0.0 0.0 3.9863539132840464E-5 5 0.0 3.9863539132840464E-5 0.0 0.0 3.9863539132840464E-5 6 0.0 3.9863539132840464E-5 0.0 0.0 7.972707826568093E-5 7 0.0 3.9863539132840464E-5 0.0 0.0 7.972707826568093E-5 8 0.0 3.9863539132840464E-5 0.0 0.0 7.972707826568093E-5 9 0.0 3.9863539132840464E-5 0.0 0.0 7.972707826568093E-5 10-11 0.0 3.9863539132840464E-5 0.0 0.0 7.972707826568093E-5 12-13 0.0 3.9863539132840464E-5 0.0 0.0 2.9897654349630347E-4 14-15 0.0 3.9863539132840464E-5 0.0 0.0 3.189083130627237E-4 16-17 0.0 3.9863539132840464E-5 0.0 0.0 3.189083130627237E-4 18-19 0.0 3.9863539132840464E-5 0.0 0.0 3.5877185219556414E-4 20-21 0.0 3.9863539132840464E-5 0.0 5.97953086992607E-5 4.584307000276653E-4 22-23 0.0 3.9863539132840464E-5 0.0 1.195906173985214E-4 4.982942391605058E-4 24-25 0.0 3.9863539132840464E-5 0.0 4.7836246959408554E-4 5.18226008726926E-4 26-27 0.0 3.9863539132840464E-5 0.0 9.367931696217508E-4 5.18226008726926E-4 28-29 0.0 3.9863539132840464E-5 0.0 0.003288741978459338 5.18226008726926E-4 30-31 0.0 3.9863539132840464E-5 0.0 0.011759744044187935 5.18226008726926E-4 32-33 0.0 3.9863539132840464E-5 0.0 0.03045574389749011 5.580895478597665E-4 34-35 0.0 3.9863539132840464E-5 0.0 0.05889837906877178 5.580895478597665E-4 36-37 0.0 3.9863539132840464E-5 0.0 0.10474144907153832 5.580895478597665E-4 38-39 0.0 3.9863539132840464E-5 0.0 0.17336653168872318 5.580895478597665E-4 40-41 0.0 3.9863539132840464E-5 0.0 0.2441442454190814 5.580895478597665E-4 42-43 0.0 3.9863539132840464E-5 0.0 0.31729383972784364 5.780213174261866E-4 44-45 0.0 3.9863539132840464E-5 0.0 0.40658816738540626 6.178848565590272E-4 46-47 0.0 3.9863539132840464E-5 0.0 0.5114093435352103 6.577483956918676E-4 48-49 0.0 3.9863539132840464E-5 0.0 0.6217516198549127 6.776801652582878E-4 50-51 0.0 3.9863539132840464E-5 0.0 0.7380534952749747 7.574072435239688E-4 52-53 0.0 3.9863539132840464E-5 0.0 0.8508473792513468 8.769978609224901E-4 54-55 0.0 9.965884783210116E-5 0.0 0.9685843420801912 9.567249391881711E-4 56-57 0.0 1.1959061739852138E-4 0.0 1.1064125286319868 9.567249391881711E-4 58-59 0.0 1.395223869649416E-4 0.0 1.2489246810318917 9.567249391881711E-4 60-61 0.0 1.5945415653136186E-4 0.0 1.3954231873450804 9.965884783210116E-4 62-63 0.0 1.5945415653136186E-4 0.0 1.5428385550583243 0.0010763155565866925 64-65 0.0 1.5945415653136186E-4 0.0 1.6980073811329057 0.0010763155565866925 66-67 0.0 1.5945415653136186E-4 0.0 1.8747623136479206 0.0010763155565866925 68-69 0.0 1.5945415653136186E-4 0.0 2.0657684614029255 0.0010763155565866925 70-71 0.0 1.5945415653136186E-4 0.0 2.26707933402377 0.001116179095719533 72-73 0.0 1.5945415653136186E-4 0.0 2.4729545818753245 0.0011759744044187936 74-75 0.0 1.5945415653136186E-4 0.0 2.6762386996832443 0.0012557014826844745 76-77 0.0 1.5945415653136186E-4 0.0 2.8940530775050846 0.0013154967913837352 78-79 0.0 1.5945415653136186E-4 0.0 3.1339319242369523 0.0013553603305165756 80-81 0.0 1.5945415653136186E-4 0.0 3.3900152996263193 0.0013553603305165756 82-83 0.0 1.5945415653136186E-4 0.0 3.64797226135493 0.0014350874087822566 84-85 0.0 1.5945415653136186E-4 0.0 3.9091581697533004 0.0014350874087822566 86-87 0.0 1.5945415653136186E-4 0.0 4.182223412813258 0.0014948827174815172 88-89 0.0 1.5945415653136186E-4 0.0 4.472709022474266 0.001554678026180778 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7985 0.0 30.195505 1 GTATCAA 11180 0.0 28.07446 1 ATCAACG 13140 0.0 23.521584 3 TCAACGC 13240 0.0 23.34393 4 CAACGCA 13315 0.0 23.176727 5 TATCAAC 13430 0.0 23.123121 2 AACGCAG 13830 0.0 22.39046 6 GAGTACT 8770 0.0 21.716127 12-13 TAAGGTG 1450 0.0 21.315424 5 GTACATG 6955 0.0 21.265348 1 TACATGG 6650 0.0 20.81041 2 GTACTTT 9270 0.0 20.314262 14-15 ACGCAGA 15145 0.0 20.289557 7 AGAGTAC 11760 0.0 20.011236 10-11 CGCAGAG 15315 0.0 20.002316 8 GTACGGG 145 6.0998445E-4 19.678524 1 GTAAGGT 1490 0.0 19.147568 4 AGTACTT 9340 0.0 19.017889 12-13 ACATGGG 6970 0.0 18.487795 3 GCAGAGT 16285 0.0 18.460928 9 >>END_MODULE