FastQCFastQC Report
Thu 26 May 2016
SRR938090_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938090_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2470771
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83160.33657510145618513No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68530.2773628150888933No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40340.16326887437160303No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA29840.12077201812713521No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT29670.12008397378793906No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA26360.10668734577182588No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA25560.10344949005796167No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70100.035.815911
GTATCAA103900.030.9636841
ATCAACG121450.026.1788163
TCAACGC121300.026.172014
TATCAAC122700.025.912122
CAACGCA122350.025.9085565
AACGCAG126500.025.243376
TACATGG61900.022.725972
TAAGGTG15500.022.6893165
GAGTACT86950.022.42587712-13
ACGCAGA139800.022.4252977
CGCAGAG140400.022.2279688
GTACATG66000.022.130221
GTACTTT90700.021.29220814-15
GTGTAGC17500.021.2747691
GTAAGGT13950.021.1221874
GTGTAAG11350.021.0272461
ACTAACG1604.9721406E-520.792153
ACATGGG64850.020.5929093
AGAGTAC113550.020.0993210-11
Produced by FastQC (version 0.11.5)