FastQCFastQC Report
Thu 26 May 2016
SRR938090_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938090_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2470771
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82130.3324063622245849No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70480.2852550883914373No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43880.17759638590545218No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT30090.12178384803771779No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA28950.11716990364546127No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA27970.11320353039597762No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA26700.10806343445021818No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA25420.10288286530803542No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC24730.10009021475482754No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA80950.028.6945291
GTATCAA112850.027.1918071
CCCGTAC1652.4328165E-623.0291563
TCAACGC132300.023.0119044
ATCAACG132250.022.985623
CAACGCA133450.022.81365
GTGTAGC17350.022.7240491
TATCAAC135000.022.589592
AACGCAG138750.021.9421626
GAGTACT86500.020.94752912-13
AGAGTAC115700.020.68957510-11
GTAAGGT13650.020.5292824
GTACTTT88850.020.36675814-15
GTGTAAG12250.020.1638931
TAAGGTG15900.020.0139165
ACGCAGA150950.020.0114427
CGCAGAG151700.019.8811978
GTAGCCC15100.019.8168933
ACCGTAC1200.00476751219.7898798
AGTACTT90600.019.65882112-13
Produced by FastQC (version 0.11.5)