FastQCFastQC Report
Thu 26 May 2016
SRR938089_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938089_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2521188
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86050.3413073519309151No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71210.2824462118652No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43990.17448123662336962No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT30210.11982446370520565No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA29310.1162547180138887No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA28200.11185203166126445No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA26380.10463321259660129No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA25220.10003220703890389No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79500.032.330231
GTATCAA113050.027.5953711
CAACGCA133100.023.1338145
ATCAACG133250.023.036453
TATCAAC134400.023.0161152
TCAACGC133200.023.0094224
AACGCAG136250.022.56416
TAAGGTG13550.021.7421595
GAGTACT89000.021.6057112-13
GTACATG72100.021.402321
TACATGG69150.021.164542
GTAAGGT12500.020.9075414
GTACTTT90850.020.81054114-15
ACGCAGA152550.019.9461737
ACATGGG69850.019.8643
GTATAGC11100.019.7983251
AGAGTAC120000.019.69076510-11
CGCAGAG153450.019.674278
GTGTAAG11850.018.9484271
AGTACTT92500.018.24427812-13
Produced by FastQC (version 0.11.5)