##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938089_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2521188 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.706721196515293 33.0 31.0 34.0 30.0 34.0 2 31.90788826537331 33.0 31.0 34.0 30.0 34.0 3 31.980281518077984 34.0 31.0 34.0 30.0 34.0 4 35.51017972479641 37.0 35.0 37.0 33.0 37.0 5 35.51960425005989 37.0 35.0 37.0 33.0 37.0 6 35.53581684507462 37.0 35.0 37.0 33.0 37.0 7 35.46542661634118 37.0 35.0 37.0 33.0 37.0 8 35.44624200971923 37.0 35.0 37.0 33.0 37.0 9 37.08435388396264 39.0 37.0 39.0 33.0 39.0 10-11 36.995281589472896 39.0 37.0 39.0 33.0 39.0 12-13 36.89431946368141 39.0 37.0 39.0 33.0 39.0 14-15 38.11452497790724 40.0 38.0 41.0 33.0 41.0 16-17 38.024240953074504 40.0 38.0 41.0 33.0 41.0 18-19 37.959160126099285 40.0 38.0 41.0 33.0 41.0 20-21 37.839981588045006 40.0 38.0 41.0 32.5 41.0 22-23 37.685162114050996 40.0 38.0 41.0 32.0 41.0 24-25 37.62852095916686 40.0 37.5 41.0 32.0 41.0 26-27 37.6396712581529 40.0 38.0 41.0 32.0 41.0 28-29 37.54956929035042 40.0 37.5 41.0 32.0 41.0 30-31 37.36195694252075 40.0 37.0 41.0 31.5 41.0 32-33 37.13684044981969 40.0 37.0 41.0 31.0 41.0 34-35 36.923642148066705 40.0 36.0 41.0 30.0 41.0 36-37 36.69852863015372 39.5 36.0 41.0 30.0 41.0 38-39 36.44776906759829 39.0 36.0 41.0 30.0 41.0 40-41 36.22003714122073 39.0 35.0 41.0 29.5 41.0 42-43 35.93756118147476 39.0 35.0 40.5 28.5 41.0 44-45 35.71667225926825 39.0 35.0 40.0 27.0 41.0 46-47 35.587734829770724 39.0 35.0 41.0 26.5 41.0 48-49 35.33849320241093 39.0 35.0 41.0 26.0 41.0 50-51 34.70181973736191 38.5 34.0 39.5 24.5 40.5 52-53 34.794341596104694 38.5 34.5 40.0 25.5 41.0 54-55 34.93519840646552 39.0 35.0 40.0 25.0 41.0 56-57 34.71905109813311 38.5 34.0 40.0 24.0 41.0 58-59 34.45475030025528 38.0 34.0 40.0 23.5 41.0 60-61 34.07163309519163 38.0 34.0 40.0 22.5 41.0 62-63 33.65301754569671 37.0 33.5 40.0 21.5 41.0 64-65 33.21810551216332 36.5 33.0 39.5 19.5 41.0 66-67 32.777635384588535 36.0 32.5 39.0 18.0 41.0 68-69 32.28016891243334 35.5 32.0 39.0 15.0 40.5 70-71 31.78083129857829 35.0 31.0 38.5 10.0 40.0 72-73 31.28844596277628 35.0 31.0 37.5 7.5 40.0 74-75 30.756187162559872 35.0 30.5 37.0 4.5 39.0 76-77 30.20771517237112 34.5 30.0 36.5 2.0 39.0 78-79 29.6253401174367 34.0 29.0 36.0 2.0 38.5 80-81 29.078310106188034 34.0 29.0 35.0 2.0 37.0 82-83 28.52635027613966 34.0 28.0 35.0 2.0 37.0 84-85 28.0226381372591 34.0 27.0 35.0 2.0 36.0 86-87 27.521564635402044 33.0 26.0 35.0 2.0 36.0 88-89 26.948396946201555 33.0 25.5 35.0 2.0 35.5 90-91 26.323142106023035 32.5 24.5 35.0 2.0 35.0 92-93 25.69871386029126 32.0 22.0 34.5 2.0 35.0 94-95 25.07904408556601 32.0 19.0 34.0 2.0 35.0 96-97 24.21855649003565 31.5 8.5 34.0 2.0 35.0 98-99 23.123586380706236 31.0 2.0 34.0 2.0 35.0 100-101 20.298311153313435 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5145795727827611 1101 2 0.4654073901261988 1101 3 0.4404665097663063 1101 4 0.3026764782068767 1101 5 0.34092085451457166 1101 6 0.33698167983150284 1101 7 0.4703163017426064 1101 8 0.3706631901633557 1101 9 0.4452792619895547 1101 10-11 0.47715520446509174 1101 12-13 0.38560842331903444 1101 14-15 0.5287925063017553 1101 16-17 0.6289549806482526 1101 18-19 0.6394788876223885 1101 20-21 0.6754558630525622 1101 22-23 0.6430558071436607 1101 24-25 0.6017383908263838 1101 26-27 0.5601749688330528 1101 28-29 0.7045028145009979 1101 30-31 0.6932726816192485 1101 32-33 0.6151259318624298 1101 34-35 0.670596304986752 1101 36-37 0.719037814092303 1101 38-39 0.588112853283107 1101 40-41 0.7737613294905259 1101 42-43 0.6005239960831759 1101 44-45 0.5781079100514077 1101 46-47 0.6455078575413182 1101 48-49 0.761737679040948 1101 50-51 0.7673985972775483 1101 52-53 0.8306371093493468 1101 54-55 0.77318966072432 1101 56-57 0.6280502155495782 1101 58-59 0.6482729861143284 1101 60-61 0.6569524801813884 1101 62-63 0.6336114547958545 1101 64-65 0.6334805346186059 1101 66-67 0.47540653428531954 1101 68-69 0.6270998775028431 1101 70-71 0.7681784118484067 1101 72-73 0.72929059358421 1101 74-75 0.731149276143011 1101 76-77 0.7232337227685832 1101 78-79 0.7322766942423868 1101 80-81 0.6681258153127274 1101 82-83 0.6967587115340059 1101 84-85 0.6708444197145234 1101 86-87 0.7090496605399963 1101 88-89 0.6109914298927848 1101 90-91 0.7603850263136387 1101 92-93 0.8477984173959676 1101 94-95 1.023561624189803 1101 96-97 1.0625334414028416 1101 98-99 1.1641989150746355 1101 100-101 1.198640182561494 1102 1 0.3306050003019614 1102 2 0.2855923867479184 1102 3 0.20241468406662122 1102 4 0.18376571243125284 1102 5 0.1849746025042549 1102 6 0.18093645843507744 1102 7 0.12597801676786702 1102 8 0.23314047002784122 1102 9 0.1981232043969854 1102 10-11 0.261353409693335 1102 12-13 0.2777684301707595 1102 14-15 0.30586396410178196 1102 16-17 0.20395331529801553 1102 18-19 0.1927772327400561 1102 20-21 0.24251999331150387 1102 22-23 0.2313569595660283 1102 24-25 0.23669266506674802 1102 26-27 0.26970553038916023 1102 28-29 0.32642500933746277 1102 30-31 0.3148299268444532 1102 32-33 0.42410026643604937 1102 34-35 0.35233550162179483 1102 36-37 0.4127389836439903 1102 38-39 0.31521333575886956 1102 40-41 0.40348354906933537 1102 42-43 0.36434735476155566 1102 44-45 0.41425267181116254 1102 46-47 0.5493162756489127 1102 48-49 0.4267953001594833 1102 50-51 0.43788335694372194 1102 52-53 0.3214971913797413 1102 54-55 0.34854866265612827 1102 56-57 0.2812657689692486 1102 58-59 0.24457543275174487 1102 60-61 0.29615482499453094 1102 62-63 0.3499138062704077 1102 64-65 0.41439371753115495 1102 66-67 0.37831566325956345 1102 68-69 0.2918943732823749 1102 70-71 0.30659566297370233 1102 72-73 0.4168689839994073 1102 74-75 0.39266683845090355 1102 76-77 0.4145049084027974 1102 78-79 0.4602874095816887 1102 80-81 0.524411322959061 1102 82-83 0.5743088385764388 1102 84-85 0.48209838086904 1102 86-87 0.4471871328238102 1102 88-89 0.6185226195238371 1102 90-91 0.7801080071954196 1102 92-93 0.7085846530382689 1102 94-95 0.6650740477026424 1102 96-97 0.7911555781899544 1102 98-99 0.975038917739198 1102 100-101 0.8192814851508743 1103 1 0.37796696880916514 1103 2 0.4026716567911244 1103 3 0.38992879010066517 1103 4 0.3296203891344902 1103 5 0.26085757252633357 1103 6 0.22073759269563453 1103 7 0.300777101703801 1103 8 0.3393003606823797 1103 9 0.39685081755672513 1103 10-11 0.4176722809939477 1103 12-13 0.3186242895238962 1103 14-15 0.47540775013423797 1103 16-17 0.42026350179558136 1103 18-19 0.4962203689470215 1103 20-21 0.415717535566408 1103 22-23 0.4387169238879238 1103 24-25 0.5736307032401626 1103 26-27 0.34017304723965935 1103 28-29 0.5285589250280651 1103 30-31 0.6307673529288351 1103 32-33 0.6778663292591318 1103 34-35 0.7364758957060218 1103 36-37 0.5722280788897294 1103 38-39 0.6771330925121646 1103 40-41 0.7644938390262013 1103 42-43 0.6454681999138643 1103 44-45 0.6104764977828765 1103 46-47 0.6802215354617474 1103 48-49 0.8278393723751662 1103 50-51 0.795717193730809 1103 52-53 0.7411875898876161 1103 54-55 0.8469383629491674 1103 56-57 0.8446596852737542 1103 58-59 0.9138594872005896 1103 60-61 0.9142721185860125 1103 62-63 0.98155483681888 1103 64-65 0.7844320498323043 1103 66-67 0.811589111776378 1103 68-69 0.7412773189292281 1103 70-71 0.9459542514494608 1103 72-73 0.9057316720272013 1103 74-75 0.7322457970328777 1103 76-77 0.8290463562190489 1103 78-79 1.0084417535179462 1103 80-81 0.967826942243299 1103 82-83 0.8893987289962517 1103 84-85 0.9013425321687798 1103 86-87 0.9410870066142998 1103 88-89 0.9706902994175266 1103 90-91 0.9833436831385178 1103 92-93 1.1487357072216895 1103 94-95 1.1981392234480275 1103 96-97 1.2015020494696174 1103 98-99 1.2723710040238885 1103 100-101 1.259018792428595 1104 1 0.4677207405580752 1104 2 0.42102453720676536 1104 3 0.3895426368912105 1104 4 0.3792470620945352 1104 5 0.2959467229550725 1104 6 0.3951117611323127 1104 7 0.3870199233242744 1104 8 0.38503865209978727 1104 9 0.3621676581462481 1104 10-11 0.5075875769706286 1104 12-13 0.3635093841468304 1104 14-15 0.49054416927293687 1104 16-17 0.6317081218160325 1104 18-19 0.7296304027739069 1104 20-21 0.7207441113895925 1104 22-23 0.722822991179136 1104 24-25 0.7755373560887691 1104 26-27 0.7794284795499777 1104 28-29 0.7822190007537628 1104 30-31 0.7774559779976684 1104 32-33 0.8943757470878566 1104 34-35 0.9192289731316166 1104 36-37 0.8470126847500978 1104 38-39 1.0119857283754925 1104 40-41 1.0126194226169147 1104 42-43 0.9814737373987725 1104 44-45 0.9564523298592533 1104 46-47 0.9944731199212171 1104 48-49 1.103348913335111 1104 50-51 1.0288730983861925 1104 52-53 0.9331130886545935 1104 54-55 1.0138954996008849 1104 56-57 1.0081499938200835 1104 58-59 1.031735661650174 1104 60-61 1.0282939435960259 1104 62-63 1.0332419056458164 1104 64-65 0.9383216284840898 1104 66-67 1.0618810353939736 1104 68-69 1.1504553837851859 1104 70-71 1.1893166232305141 1104 72-73 1.1480267354910865 1104 74-75 1.17253878094715 1104 76-77 1.2526683125690283 1104 78-79 1.3276388524094216 1104 80-81 1.4217289195255844 1104 82-83 1.4640720892132393 1104 84-85 1.4644512194188835 1104 86-87 1.4150733116855996 1104 88-89 1.3479278674389796 1104 90-91 1.396730185219777 1104 92-93 1.5389477152525792 1104 94-95 1.6317101829481153 1104 96-97 1.5925408323858434 1104 98-99 1.5002299572032385 1104 100-101 1.3415633163382878 1105 1 0.39337727114473253 1105 2 0.39074022546684617 1105 3 0.3462313888321127 1105 4 0.28261578973198453 1105 5 0.25006962895281504 1105 6 0.23486284369651855 1105 7 0.13994356999846502 1105 8 0.22907755690165033 1105 9 0.18471055083495713 1105 10-11 0.2721298620732 1105 12-13 0.1660159181089611 1105 14-15 0.34727390916819445 1105 16-17 0.358919060295527 1105 18-19 0.3077758666128858 1105 20-21 0.36011689924558965 1105 22-23 0.24371599815191303 1105 24-25 0.2312573456730007 1105 26-27 0.25056379766472503 1105 28-29 0.24162983507563496 1105 30-31 0.3351236187310107 1105 32-33 0.3535331794095029 1105 34-35 0.27964039575117994 1105 36-37 0.37751784923234766 1105 38-39 0.22759876350940544 1105 40-41 0.26563300168508874 1105 42-43 0.23581387955464805 1105 44-45 0.23875132010893907 1105 46-47 0.24288953824909498 1105 48-49 0.07276225515535373 1105 50-51 0.04925640809117482 1105 52-53 0.0030525229243849594 1105 54-55 -0.08167948906714884 1105 56-57 0.06188110319523332 1105 58-59 0.0025047403374074406 1105 60-61 0.1414657843891618 1105 62-63 0.12349545230939896 1105 64-65 0.07006993351161839 1105 66-67 0.20677200743536872 1105 68-69 0.18482547125829996 1105 70-71 0.41821843538030734 1105 72-73 0.3872280972555906 1105 74-75 0.4645408255227572 1105 76-77 0.4258426608605532 1105 78-79 0.4189887062994231 1105 80-81 0.2314461990827219 1105 82-83 0.351676608496863 1105 84-85 0.447387210700505 1105 86-87 0.5049174610167704 1105 88-89 0.43698094925093756 1105 90-91 0.3207358656680377 1105 92-93 0.48477297154784793 1105 94-95 0.5997306576368509 1105 96-97 0.7215024387278852 1105 98-99 0.5789687819580323 1105 100-101 0.6231469462315218 1106 1 0.08474548760805334 1106 2 0.03722671882105644 1106 3 -0.02361522431122509 1106 4 0.03278390232186723 1106 5 0.13723555747350957 1106 6 0.1909479616573293 1106 7 0.28207943348050435 1106 8 0.06532830217497576 1106 9 0.19456628699146705 1106 10-11 -0.33585419992362375 1106 12-13 -0.12190648607771237 1106 14-15 0.0355648130530426 1106 16-17 0.1896973921476075 1106 18-19 0.32720348430409274 1106 20-21 0.21746499983672152 1106 22-23 0.1399277010193387 1106 24-25 0.12056915005874203 1106 26-27 0.12327707908613661 1106 28-29 0.047030912979472816 1106 30-31 0.09731828908159201 1106 32-33 0.15251793430909544 1106 34-35 -0.11754976887210233 1106 36-37 -0.1037984119188664 1106 38-39 -0.03795660921402089 1106 40-41 0.06042315378901719 1106 42-43 -0.012922177171653004 1106 44-45 0.04236358709093935 1106 46-47 0.38403431216323014 1106 48-49 0.28256591514461604 1106 50-51 0.37725313508624936 1106 52-53 0.24469972881566804 1106 54-55 -0.05419966567678358 1106 56-57 0.04988770312507995 1106 58-59 0.16977118934087088 1106 60-61 -0.13649885401846973 1106 62-63 0.06694160236065727 1106 64-65 0.10593733559727525 1106 66-67 -0.04950649423367537 1106 68-69 -0.45962980366543604 1106 70-71 -0.22956128537615683 1106 72-73 0.08974055153206706 1106 74-75 0.03694482626152151 1106 76-77 0.13430777277240935 1106 78-79 0.304858695924473 1106 80-81 0.28681512642213747 1106 82-83 0.23418798747253433 1106 84-85 0.1354126206473154 1106 86-87 0.2767325873692634 1106 88-89 0.14329894575791258 1106 90-91 -0.01716199562993026 1106 92-93 0.16002507845567848 1106 94-95 -0.006874571237982963 1106 96-97 0.2522946999088447 1106 98-99 0.1742140156174905 1106 100-101 -0.2025850640169935 1107 1 0.24510209880686773 1107 2 0.173978947937119 1107 3 0.11492556771333895 1107 4 0.042994639502303755 1107 5 0.1703173096907804 1107 6 0.12692519619148612 1107 7 0.12173140833107965 1107 8 0.07361369179805166 1107 9 0.12194740573593776 1107 10-11 0.06652567532403708 1107 12-13 0.054522987520485344 1107 14-15 0.19036402976847455 1107 16-17 0.1288516129902746 1107 18-19 0.24206333037103178 1107 20-21 0.27172935008595545 1107 22-23 0.3229789431542258 1107 24-25 0.33040649771557895 1107 26-27 0.29780163400309334 1107 28-29 0.2729821288232799 1107 30-31 0.34550048919457055 1107 32-33 0.3599731713847234 1107 34-35 0.3526228136812861 1107 36-37 0.46029156158873974 1107 38-39 0.35317517124839526 1107 40-41 0.4709585916716179 1107 42-43 0.592092830650536 1107 44-45 0.5731673715846526 1107 46-47 0.566648553991385 1107 48-49 0.5215136711065966 1107 50-51 0.47217361346539377 1107 52-53 0.442051110222188 1107 54-55 0.6217774859575869 1107 56-57 0.7549685274568745 1107 58-59 0.6919390365399849 1107 60-61 0.6911338359730337 1107 62-63 0.7644031220558674 1107 64-65 0.6155022686320137 1107 66-67 0.6561045926654359 1107 68-69 0.6532033942649136 1107 70-71 0.7756650280821482 1107 72-73 0.7342048294216283 1107 74-75 0.7294174071569088 1107 76-77 0.8907060819720343 1107 78-79 0.8292537347274944 1107 80-81 0.9815419153699381 1107 82-83 0.863625272599247 1107 84-85 0.9657791531742319 1107 86-87 1.1015127845049584 1107 88-89 1.1698413851196783 1107 90-91 1.3068939386329035 1107 92-93 1.3982717390746124 1107 94-95 1.326990850431784 1107 96-97 1.3867536396442546 1107 98-99 1.2153704291595275 1107 100-101 1.2571438477575043 1108 1 0.26657438057511484 1108 2 0.2405061750755344 1108 3 0.20354711862168173 1108 4 0.26105901461041725 1108 5 0.25046549251634076 1108 6 0.1948108184873547 1108 7 0.13229651276442667 1108 8 0.2288753467309732 1108 9 0.2707912853847958 1108 10-11 0.31008817885592777 1108 12-13 0.10693587080348976 1108 14-15 0.23902278845547897 1108 16-17 0.34327682007704396 1108 18-19 0.32645013349969076 1108 20-21 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pass #Quality Count 2 8590.0 3 2570.0 4 2527.0 5 2894.0 6 3252.0 7 3823.0 8 4739.0 9 5864.0 10 7238.0 11 8658.0 12 10638.0 13 12683.0 14 14683.0 15 17427.0 16 20449.0 17 25391.0 18 23554.0 19 19733.0 20 19675.0 21 20568.0 22 22439.0 23 24497.0 24 27102.0 25 30351.0 26 33979.0 27 39230.0 28 45626.0 29 53966.0 30 64005.0 31 77982.0 32 96715.0 33 124445.0 34 169042.0 35 243165.0 36 358686.0 37 478277.0 38 353760.0 39 42957.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.39385450923482 18.497693637404648 14.50347937307114 23.604972480289398 2 15.21977053895409 19.420061650989368 40.84274870713875 24.51741910291779 3 19.407579731557952 23.331305393803238 29.735268567056337 27.52584630758247 4 12.751726864148061 16.257288866945334 37.034111817926366 33.956872450980235 5 13.641500468990833 37.100687945843966 34.59572513763085 14.662086447534357 6 32.78677979709423 35.95639324695683 16.998192184343992 14.258634771604944 7 28.59312797431226 32.64009779757014 20.411829378167802 18.354944849949792 8 26.12635743665957 35.29988114678471 19.322596198681303 19.251165217874412 9 25.858262142179132 16.980479179166785 20.3090823087204 36.85217636993368 10-11 25.036824813911146 26.33532868056622 27.140731320362583 21.487115185160047 12-13 25.986416706537728 23.61688441891814 27.537894053487445 22.858804821056687 14-15 22.490214683669286 25.31998966603925 25.91300186082727 26.276793789464204 16-17 22.083551941758746 28.370148182778603 26.920784244001133 22.625515631461514 18-19 22.121418671079798 26.950087359770453 29.059520726738043 21.868973242411705 20-21 22.54359236536474 26.1582771164369 29.351878442501384 21.946252075696975 22-23 22.227732301433743 26.06336260356204 29.3115642208662 22.397340874138017 24-25 22.204373120377426 26.25482020034883 29.391118901815094 22.14968777745864 26-27 21.845662736030185 26.52581307141526 29.253702968347927 22.374821224206627 28-29 21.882511886554276 27.08092342735216 28.988110369774585 22.048454316318974 30-31 22.127492364389774 26.498276406113252 28.91601126438561 22.458219965111365 32-33 21.753152743723042 26.543537086228746 29.035189472232336 22.668120697815876 34-35 22.486764611321348 26.628038145862753 28.821512577290783 22.063684665525116 36-37 22.548224835102946 26.228440145400334 28.878402350600403 22.34493266889632 38-39 21.989364781487588 26.71799569202823 28.796531560942405 22.496107965541782 40-41 22.52228765569218 26.529819845665898 28.788841946623904 22.159050552018023 42-43 22.16240924969164 26.538839336465532 28.59655725674116 22.702194157101673 44-45 22.236746683001066 26.753792272792914 28.738001120345054 22.27145992386097 46-47 22.70171211469881 26.93388301710052 28.11568195654835 22.248722911652326 48-49 22.362594570317786 27.027144443576002 28.089581385125605 22.520679600980607 50-51 22.3126065623038 27.23630820074726 28.20493288620559 22.246152350743344 52-53 22.707158077737766 27.014113183982392 27.981937282760793 22.296791455519053 54-55 22.315497349795283 27.11262576570286 28.162471831656454 22.4094050528454 56-57 22.391313791771662 27.183022569882848 27.985075737123022 22.440587901222468 58-59 22.460618496006806 26.902346723857967 27.946437108014837 22.69059767212039 60-61 22.138585586232534 27.121162407513133 28.26500609206215 22.475245914192186 62-63 22.13769030838324 27.077907793477035 28.426850151655596 22.35755174648413 64-65 21.902163348051875 27.24798958990411 28.241630068673306 22.608216993370707 66-67 21.98825602531278 27.15645383059701 28.02608476258558 22.82920538150463 68-69 22.34919855351053 26.840171085801355 28.267142350202235 22.54348801048588 70-71 22.26611657641232 26.87784762473501 27.934433579974545 22.92160221887812 72-73 22.224151000741777 26.946852276440296 28.064752467508402 22.764244255309524 74-75 22.102777559559662 27.471716948088687 27.811621424099375 22.61388406825228 76-77 22.293030902444695 27.011491516843723 27.851356916214204 22.844120664497378 78-79 22.394200253887657 27.14503332275468 27.85109885750555 22.60966756585211 80-81 22.5224263834438 27.199312902224438 27.61807757421162 22.660183140120143 82-83 22.377052676053825 27.20713072163149 27.86061490274801 22.55520169956668 84-85 22.38224934676363 27.353252653955607 27.726737436171472 22.537760563109288 86-87 22.45037118722394 27.35847185502377 27.463233325333753 22.727923632418538 88-89 22.38571298195771 27.416801907792664 27.237390831643648 22.96009427860598 90-91 22.345768913298837 27.32920092701356 27.386623384869363 22.938406774818247 92-93 22.610580968920324 27.0510987770558 27.570918503582575 22.767401750441298 94-95 22.375643578658465 26.80592913828915 27.798768519628208 23.019658763424175 96-97 22.52459679899371 26.55990032319569 27.817843814542414 23.097659063268186 98-99 22.42750203243081 26.73750516290989 27.42256031925332 23.412432485405976 100-101 22.804426358306177 26.10397233973025 27.672427236346913 23.41917406561666 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 541.0 1 377.0 2 319.5 3 432.5 4 381.0 5 365.0 6 663.5 7 1435.0 8 2332.0 9 2776.0 10 2425.5 11 1965.5 12 1983.0 13 2134.5 14 2271.5 15 2417.5 16 2677.0 17 2856.5 18 3050.5 19 3535.5 20 4195.5 21 5097.5 22 6236.5 23 7895.0 24 10030.0 25 12689.5 26 16310.0 27 20539.5 28 23856.0 29 26926.0 30 30442.0 31 35346.5 32 42650.0 33 46570.0 34 47731.0 35 50987.0 36 55343.5 37 59797.5 38 63977.0 39 67069.0 40 71138.5 41 76621.5 42 79209.5 43 81233.5 44 84535.5 45 88416.5 46 91147.5 47 95055.0 48 100301.0 49 98732.5 50 93566.5 51 90059.0 52 87600.5 53 86995.0 54 86537.5 55 81502.5 56 74482.0 57 68379.0 58 63506.5 59 56201.0 60 44904.0 61 36513.0 62 30720.5 63 22937.5 64 15857.5 65 12154.0 66 9258.0 67 6942.5 68 5236.5 69 3888.5 70 2881.5 71 1942.0 72 1299.0 73 944.0 74 689.0 75 490.5 76 328.5 77 202.0 78 120.5 79 78.0 80 60.0 81 42.5 82 27.5 83 18.0 84 16.0 85 12.0 86 7.5 87 6.0 88 4.5 89 7.0 90 7.0 91 5.0 92 4.5 93 3.5 94 1.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.48623902699838334 2 0.04743795385350081 3 0.055093075169324934 4 0.06314483489529539 5 0.0773048261375193 6 0.10625943007820121 7 0.06734920204284647 8 0.050492069611627534 9 0.04807257530973493 10-11 0.07246583753373409 12-13 0.10522817021182077 14-15 0.05318921080062257 16-17 0.05798853556339313 18-19 0.05693744377650536 20-21 0.07351692932062187 22-23 0.061994583505871045 24-25 0.08850986122415305 26-27 0.06431491820522706 28-29 0.06502886734349045 30-31 0.06913407488850494 32-33 0.06962987290118786 34-35 0.07163289687242681 36-37 0.06590147184581237 38-39 0.03873174075078891 40-41 0.04247997372667171 42-43 0.04035795823238886 44-45 0.021259818783843174 46-47 0.028101831358867325 48-49 0.028121663279374643 50-51 0.025999647785091787 52-53 0.025265866726321085 54-55 0.02490889215718939 56-57 0.02358015348319919 58-59 0.02082351653268221 60-61 0.02756636950516978 62-63 0.020208726996955403 64-65 0.022132423286165093 66-67 0.016103519451940912 68-69 0.01842385415129693 70-71 0.03948535373006694 72-73 0.03500333969541343 74-75 0.030699812945325776 76-77 0.021656457193989502 78-79 0.015389570313677521 80-81 0.017471921966945744 82-83 0.021636625273482184 84-85 0.018622173356370092 86-87 0.008845036546263112 88-89 0.039584513332603516 90-91 0.05029375040655437 92-93 0.03153275360663307 94-95 0.05132501027293482 96-97 0.041369386178261996 98-99 0.032008719698808655 100-101 0.16740124100225764 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2521188.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.97640454802527 #Duplication Level Percentage of deduplicated Percentage of total 1 76.29205574303329 43.46847031815559 2 13.407290090584572 15.277983681877542 3 4.539243273900179 7.758892833469178 4 2.0377792205620757 4.644213330012178 5 1.0751476517837224 3.062902377844438 6 0.629818740606529 2.1530884414035234 7 0.4231201319744038 1.687550466825123 8 0.28309845479466983 1.2903945669841563 9 0.20653699257902822 1.0590961718983685 >10 1.0156160263811589 10.18209094890398 >50 0.054042373498467765 2.0802151669577102 >100 0.030801962731391432 3.260998772630204 >500 0.0029342586918695963 1.162243841202351 >1k 0.002375352274363134 2.285876252645059 >5k 1.3972660437430203E-4 0.6259828291905444 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8605 0.3413073519309151 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7121 0.2824462118652 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4399 0.17448123662336962 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3021 0.11982446370520565 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2931 0.1162547180138887 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2820 0.11185203166126445 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2638 0.10463321259660129 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2522 0.10003220703890389 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 3.966384101463278E-5 0.0 0.0 0.0 8 0.0 3.966384101463278E-5 0.0 0.0 0.0 9 0.0 3.966384101463278E-5 0.0 0.0 0.0 10-11 0.0 3.966384101463278E-5 0.0 0.0 0.0 12-13 0.0 5.949576152194918E-5 0.0 0.0 2.1815112558048032E-4 14-15 0.0 9.915960253658197E-5 0.0 1.983192050731639E-5 2.379830460877967E-4 16-17 0.0 1.1899152304389835E-4 0.0 3.966384101463278E-5 2.974788076097459E-4 18-19 0.0 1.1899152304389835E-4 0.0 3.966384101463278E-5 3.9663841014632786E-4 20-21 0.0 1.5865536405853113E-4 0.0 3.966384101463278E-5 4.164703306536443E-4 22-23 0.0 1.5865536405853113E-4 0.0 7.932768202926556E-5 4.3630225116096064E-4 24-25 0.0 1.5865536405853113E-4 0.0 2.379830460877967E-4 4.759660921755934E-4 26-27 0.0 1.5865536405853113E-4 0.0 6.742852972487573E-4 4.759660921755934E-4 28-29 0.0 1.5865536405853113E-4 0.0 0.003093779599141357 4.759660921755934E-4 30-31 0.0 1.5865536405853113E-4 0.0 0.011720665019823988 4.759660921755934E-4 32-33 0.0 1.5865536405853113E-4 0.0 0.03135426632206721 5.156299331902262E-4 34-35 0.0 1.5865536405853113E-4 0.0 0.05882147622470042 5.55293774204859E-4 36-37 0.0 1.5865536405853113E-4 0.0 0.10711220266001584 5.55293774204859E-4 38-39 0.0 1.5865536405853113E-4 0.0 0.1774956885404817 5.949576152194918E-4 40-41 0.0 1.5865536405853113E-4 0.0 0.2522620288530645 5.949576152194918E-4 42-43 0.0 1.5865536405853113E-4 0.0 0.3280992928730424 5.949576152194918E-4 44-45 0.0 1.5865536405853113E-4 0.0 0.4201987317090197 5.949576152194918E-4 46-47 0.0 1.5865536405853113E-4 0.0 0.5256648849669283 6.147895357268081E-4 48-49 0.0 1.5865536405853113E-4 0.0 0.6346809519956464 6.742852972487573E-4 50-51 0.0 1.5865536405853113E-4 0.0 0.7504002081558376 6.742852972487573E-4 52-53 0.0 1.5865536405853113E-4 0.0 0.8615382906788387 7.536129792780229E-4 54-55 0.0 1.5865536405853113E-4 0.0 0.9807083010073029 7.536129792780229E-4 56-57 0.0 1.5865536405853113E-4 0.0 1.1193135934329372 7.932768202926557E-4 58-59 0.0 2.379830460877967E-4 0.0 1.262599219098298 7.932768202926557E-4 60-61 0.0 2.379830460877967E-4 0.0 1.4164354264735515 7.932768202926557E-4 62-63 0.0 2.379830460877967E-4 0.0 1.5658292836551657 7.932768202926557E-4 64-65 0.0 2.379830460877967E-4 0.0 1.7171270052054823 7.932768202926557E-4 66-67 0.0 2.379830460877967E-4 0.0 1.889982024347252 8.13108740799972E-4 68-69 0.0 2.379830460877967E-4 0.0 2.0842555176369233 8.329406613072884E-4 70-71 0.0 2.379830460877967E-4 0.0 2.2865807706525656 8.329406613072884E-4 72-73 1.983192050731639E-5 2.379830460877967E-4 0.0 2.490829719957417 9.12268343336554E-4 74-75 3.966384101463278E-5 2.379830460877967E-4 0.0 2.694305224362483 9.915960253658197E-4 76-77 3.966384101463278E-5 2.379830460877967E-4 0.0 2.915312146496017 9.915960253658197E-4 78-79 3.966384101463278E-5 2.379830460877967E-4 0.0 3.160970145820145 9.915960253658197E-4 80-81 3.966384101463278E-5 2.379830460877967E-4 0.0 3.414580745267707 0.001011427945873136 82-83 3.966384101463278E-5 2.379830460877967E-4 0.0 3.676897557817981 0.001110587548409718 84-85 3.966384101463278E-5 2.379830460877967E-4 0.0 3.943418737515806 0.001110587548409718 86-87 3.966384101463278E-5 2.379830460877967E-4 0.0 4.215711006081261 0.0011304194689170342 88-89 3.966384101463278E-5 2.379830460877967E-4 0.0 4.506169313831416 0.0011502513894243508 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7950 0.0 32.33023 1 GTATCAA 11305 0.0 27.595371 1 CAACGCA 13310 0.0 23.133814 5 ATCAACG 13325 0.0 23.03645 3 TATCAAC 13440 0.0 23.016115 2 TCAACGC 13320 0.0 23.009422 4 AACGCAG 13625 0.0 22.5641 6 TAAGGTG 1355 0.0 21.742159 5 GAGTACT 8900 0.0 21.60571 12-13 GTACATG 7210 0.0 21.40232 1 TACATGG 6915 0.0 21.16454 2 GTAAGGT 1250 0.0 20.907541 4 GTACTTT 9085 0.0 20.810541 14-15 ACGCAGA 15255 0.0 19.946173 7 ACATGGG 6985 0.0 19.864 3 GTATAGC 1110 0.0 19.798325 1 AGAGTAC 12000 0.0 19.690765 10-11 CGCAGAG 15345 0.0 19.67427 8 GTGTAAG 1185 0.0 18.948427 1 AGTACTT 9250 0.0 18.244278 12-13 >>END_MODULE