FastQCFastQC Report
Thu 26 May 2016
SRR938089_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938089_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2521188
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83620.33166903856435936No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71900.2851830168952097No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45760.1815017364829596No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT31140.1235132009195665No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA29840.11835690158766422No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA29340.11637370953693259No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA26790.10625943007820121No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC25520.10122212226934285No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA25270.10023052624397705No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78800.029.6658421
GTATCAA110350.027.9010471
TCAACGC128650.023.5540224
ATCAACG129600.023.4212653
TATCAAC131100.023.3037682
TAAGGTG15950.023.2267325
CAACGCA130700.023.220925
AACGCAG134500.022.6001766
GTACATG69600.021.162661
GAGTACT85500.021.08141912-13
GTGTAGC18350.020.97331
GTAAGGT15600.020.7032474
TACATGG66900.020.6673472
ACGCAGA148950.020.407687
CGCAGAG148100.020.3965268
AGAGTAC116300.020.35821210-11
GTACTTT88850.020.12619614-15
GTGTAAG12250.019.7811681
GGTAAGG16350.019.4657673
AGTACTT88950.019.43612112-13
Produced by FastQC (version 0.11.5)