FastQCFastQC Report
Thu 26 May 2016
SRR938088_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938088_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2417802
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78830.3260399321367093No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65220.26974913578531245No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39640.16395056336292219No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT29310.12122580757233223No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA28160.1164694214000981No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA26090.10790792629007669No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA25550.10567449278311458No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74400.032.132511
GTATCAA105550.028.0637741
ATCAACG120050.024.0110233
TCAACGC120700.023.8817164
TATCAAC122000.023.7437152
CAACGCA121600.023.6658425
GTGTAGC17150.023.6030831
AACGCAG125100.023.150066
GAGTACT76700.022.90048412-13
TAAGGTG13000.021.9537185
TACATGG64300.021.8223862
GTACTTT81100.021.53902414-15
GTACATG69150.021.280421
GTGTAAG9150.020.818661
ACGCAGA137850.020.7958457
CGCAGAG137500.020.7446278
TAGCCCT17200.020.741164
TAGGACG1150.003694429520.681044
AGAGTAC103250.019.93553510-11
ACATGGG67250.019.8045963
Produced by FastQC (version 0.11.5)