##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938088_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2417802 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.798954587679223 33.0 31.0 34.0 30.0 34.0 2 31.93130372131382 33.0 31.0 34.0 30.0 34.0 3 31.95360041889286 34.0 31.0 34.0 30.0 34.0 4 35.47606710557771 37.0 35.0 37.0 33.0 37.0 5 35.47209821151608 37.0 35.0 37.0 33.0 37.0 6 35.49123170549119 37.0 35.0 37.0 33.0 37.0 7 35.41674545723761 37.0 35.0 37.0 33.0 37.0 8 35.403046237863975 37.0 35.0 37.0 33.0 37.0 9 37.01762427196272 39.0 37.0 39.0 33.0 39.0 10-11 36.915065212122414 39.0 37.0 39.0 33.0 39.0 12-13 36.75921208601862 39.0 37.0 39.0 33.0 39.0 14-15 37.91055450363595 40.0 38.0 41.0 33.0 41.0 16-17 37.72566922353444 40.0 38.0 41.0 32.0 41.0 18-19 37.89422996589464 40.0 38.0 41.0 33.0 41.0 20-21 37.88307044993759 40.0 38.0 41.0 32.5 41.0 22-23 37.764584734399264 40.0 38.0 41.0 32.0 41.0 24-25 37.63492874933514 40.0 38.0 41.0 32.0 41.0 26-27 37.45028211573984 40.0 37.0 41.0 32.0 41.0 28-29 37.26446603154435 40.0 37.0 41.0 31.0 41.0 30-31 37.04577794211437 40.0 37.0 41.0 31.0 41.0 32-33 36.74633613505159 39.5 36.0 41.0 30.0 41.0 34-35 36.49819588204493 39.0 36.0 41.0 30.0 41.0 36-37 36.22217907008101 39.0 35.0 41.0 29.5 41.0 38-39 35.91597947226448 39.0 35.0 40.0 28.5 41.0 40-41 35.804958181025576 39.0 35.0 40.0 27.5 41.0 42-43 35.58519473472187 39.0 35.0 40.0 27.0 41.0 44-45 35.25419327140932 38.5 35.0 40.0 25.5 41.0 46-47 34.88900662667994 38.0 34.0 40.0 24.5 41.0 48-49 34.48312930504648 38.0 34.0 40.0 23.5 41.0 50-51 33.76248882249249 37.5 33.0 39.5 22.5 40.5 52-53 33.84934002039869 38.0 33.0 39.5 22.5 40.5 54-55 33.9233758595617 38.0 33.0 40.0 21.5 41.0 56-57 33.5966834753218 38.0 33.0 40.0 19.5 41.0 58-59 33.1707550907808 37.5 32.5 40.0 16.5 41.0 60-61 32.64543229759923 37.0 32.0 40.0 11.0 41.0 62-63 32.09338316371647 36.0 31.0 39.0 8.0 41.0 64-65 31.534093776082575 35.5 30.5 39.0 4.5 40.0 66-67 30.94915526581581 35.0 29.5 38.5 2.0 40.0 68-69 30.25191496243282 35.0 29.0 38.0 2.0 40.0 70-71 29.636658212707246 34.0 28.0 37.5 2.0 39.5 72-73 28.920302613696244 34.0 27.0 37.0 2.0 39.0 74-75 28.22636717150536 34.0 26.0 36.0 2.0 39.0 76-77 27.467122411181727 33.0 25.5 35.5 2.0 38.5 78-79 26.685957534984254 32.5 23.5 35.0 2.0 37.0 80-81 25.933844251927994 32.0 21.0 35.0 2.0 37.0 82-83 25.17075984716697 31.5 18.5 35.0 2.0 36.0 84-85 24.424685933753054 31.0 12.5 34.0 2.0 36.0 86-87 23.665546641122802 30.5 4.5 34.0 2.0 35.0 88-89 22.766231891610644 30.0 2.0 34.0 2.0 35.0 90-91 21.846057493541657 29.0 2.0 34.0 2.0 35.0 92-93 20.89218823543036 29.0 2.0 34.0 2.0 35.0 94-95 19.79630879617107 26.5 2.0 33.5 2.0 35.0 96-97 18.519058632592746 25.0 2.0 33.0 2.0 35.0 98-99 16.964597183723065 12.5 2.0 32.5 2.0 34.0 100-101 13.196863308078992 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2530453670905928 1101 2 0.2368196383877681 1101 3 0.19660511078045673 1101 4 0.11905631400238548 1101 5 0.1307479923333119 1101 6 0.12461179858706117 1101 7 0.16635992051666904 1101 8 0.187813062988603 1101 9 0.20835419463347904 1101 10-11 0.35907657322545816 1101 12-13 0.3440808639743764 1101 14-15 0.3606665427908098 1101 16-17 0.39823782003972497 1101 18-19 0.5327791856919788 1101 20-21 0.466951658443044 1101 22-23 0.3742390847718937 1101 24-25 0.35659637062121874 1101 26-27 0.45525612546935434 1101 28-29 0.3961935871634026 1101 30-31 0.4189917756949768 1101 32-33 0.43223050676733266 1101 34-35 0.32964048755078323 1101 36-37 0.382983953791026 1101 38-39 0.21064717098402497 1101 40-41 0.5275271178106422 1101 42-43 0.563578794842698 1101 44-45 0.6626104608749372 1101 46-47 0.6061146279446632 1101 48-49 0.7343118962091921 1101 50-51 0.7735120336175711 1101 52-53 0.5563025441122775 1101 54-55 0.5430833259475776 1101 56-57 0.34044101286696815 1101 58-59 0.48877924250975724 1101 60-61 0.4707842874140056 1101 62-63 0.32833434528351546 1101 64-65 0.13521733251919343 1101 66-67 0.1702247766981806 1101 68-69 0.2804442255289139 1101 70-71 0.2908833991170958 1101 72-73 0.13101201666340145 1101 74-75 0.07174624859399614 1101 76-77 0.10560566387689008 1101 78-79 0.0020081971933336717 1101 80-81 -0.18030359336205493 1101 82-83 -0.12024344278700028 1101 84-85 -0.06873062523299112 1101 86-87 -0.21519507837406238 1101 88-89 -0.17434607452092976 1101 90-91 -0.3994941011004265 1101 92-93 -0.5266952150298927 1101 94-95 -0.5168682207581803 1101 96-97 -0.5885894611752498 1101 98-99 -0.6873876455345567 1101 100-101 -1.0278000633051096 1102 1 0.16407299307723378 1102 2 0.18184088914672358 1102 3 0.14197548115083336 1102 4 0.19425364247840093 1102 5 0.15812352360228488 1102 6 0.1312298920904027 1102 7 0.16335445603579046 1102 8 0.21163668168927074 1102 9 0.26722638649747665 1102 10-11 0.2492860449923029 1102 12-13 0.1872995950004892 1102 14-15 0.17208123617271553 1102 16-17 0.35104595604458666 1102 18-19 0.30635234901923525 1102 20-21 0.3124055139378825 1102 22-23 0.3099441545958186 1102 24-25 0.40483179867221963 1102 26-27 0.2677067933503494 1102 28-29 0.23788681727876337 1102 30-31 0.1600323039281264 1102 32-33 0.11093421047103647 1102 34-35 0.11512925500675664 1102 36-37 0.21336874431926134 1102 38-39 -0.1705610864537377 1102 40-41 0.11298932181792765 1102 42-43 0.18583517007038353 1102 44-45 0.11145305346752821 1102 46-47 0.0712709728141192 1102 48-49 -0.07885219000817045 1102 50-51 0.052117073083273624 1102 52-53 -0.009438196628458684 1102 54-55 -0.06391045061587874 1102 56-57 -0.1233757752326099 1102 58-59 -0.1702902778302544 1102 60-61 0.018344244912483987 1102 62-63 -0.04887801051491181 1102 64-65 -0.16068051204668166 1102 66-67 -0.2814714892398875 1102 68-69 -0.48345604769513173 1102 70-71 -0.6743374569849117 1102 72-73 -0.7136145771435203 1102 74-75 -0.6837523852857856 1102 76-77 -0.7856777301728961 1102 78-79 -1.0292418028066663 1102 80-81 -0.8947579042303033 1102 82-83 -1.0010418641591912 1102 84-85 -0.838774644662255 1102 86-87 -0.5013023947067872 1102 88-89 -0.6101118607990088 1102 90-91 -0.8219591891392888 1102 92-93 -0.883692786371725 1102 94-95 -0.8610356463805253 1102 96-97 -0.8857206087162375 1102 98-99 -0.8366704324198047 1102 100-101 -1.0316707372577518 1103 1 0.4267529715908793 1103 2 0.43160078316891415 1103 3 0.4339685829000324 1103 4 0.36353360726016604 1103 5 0.3579108813864451 1103 6 0.34843129435778053 1103 7 0.35571690499560304 1103 8 0.25290401314052247 1103 9 0.24427334549165636 1103 10-11 0.4668494236812393 1103 12-13 0.4625619143151809 1103 14-15 0.4879125804026998 1103 16-17 0.5937826997309514 1103 18-19 0.515999601229467 1103 20-21 0.5202478315562828 1103 22-23 0.5057159468889978 1103 24-25 0.5022649466251607 1103 26-27 0.3970085070083229 1103 28-29 0.4725367956712532 1103 30-31 0.6830885359807581 1103 32-33 0.44798767191474553 1103 34-35 0.28264204786940894 1103 36-37 0.29475871358408057 1103 38-39 0.3497310997021046 1103 40-41 0.40586691825409815 1103 42-43 0.5607409653280371 1103 44-45 0.6040189563992797 1103 46-47 0.49879652824851917 1103 48-49 0.4788266576446887 1103 50-51 0.42247832252288475 1103 52-53 0.51445315592143 1103 54-55 0.5258371856831445 1103 56-57 0.6537576138360066 1103 58-59 0.8099287456714563 1103 60-61 0.46583181554080255 1103 62-63 0.3924198137897079 1103 64-65 0.37088857748922166 1103 66-67 0.14932215289921302 1103 68-69 0.08635855171762685 1103 70-71 -0.04719710100608765 1103 72-73 -0.1317497473178726 1103 74-75 -0.012069038422509948 1103 76-77 -0.17238819750111745 1103 78-79 -0.24074094052532047 1103 80-81 -0.3731338265886315 1103 82-83 -0.3577732120240853 1103 84-85 -0.5072694689547603 1103 86-87 -0.6008037052936608 1103 88-89 -0.44247810622003314 1103 90-91 -0.5157132026853866 1103 92-93 -0.6813617995745211 1103 94-95 -0.9364753694811832 1103 96-97 -1.204011979036796 1103 98-99 -1.2042929793606234 1103 100-101 -1.4943312286167618 1104 1 0.296302842852306 1104 2 0.2088831528993076 1104 3 0.19887455247501507 1104 4 0.15059672161407178 1104 5 0.2233443169141509 1104 6 0.25997769057153874 1104 7 0.1603353129646976 1104 8 0.1888075396459925 1104 9 0.23054361464321715 1104 10-11 0.2778268678561062 1104 12-13 0.3415416388137018 1104 14-15 0.4305910387759795 1104 16-17 0.30846661056125413 1104 18-19 0.3854171121207344 1104 20-21 0.2528080122512364 1104 22-23 0.3196586584609875 1104 24-25 0.44838825531456195 1104 26-27 0.36264673892965504 1104 28-29 0.259332837465891 1104 30-31 0.29003223437597114 1104 32-33 0.4027835481413362 1104 34-35 0.40812852161793955 1104 36-37 0.3853342224941798 1104 38-39 0.2817855452513811 1104 40-41 0.33058980342794797 1104 42-43 0.4267649896289143 1104 44-45 0.5542416736532587 1104 46-47 0.19423531651614212 1104 48-49 0.2826249393116882 1104 50-51 0.24055327405189786 1104 52-53 0.38115365742330454 1104 54-55 0.35077446267190737 1104 56-57 0.3524241324948676 1104 58-59 0.34557244322001424 1104 60-61 0.2953848664614469 1104 62-63 0.340743107991905 1104 64-65 0.10312665306163282 1104 66-67 0.237291846419577 1104 68-69 0.08281276133243054 1104 70-71 -0.00630611910286305 1104 72-73 0.030335544688671945 1104 74-75 -0.04473627391578816 1104 76-77 -0.09922028797653226 1104 78-79 -0.054279515529998434 1104 80-81 -0.2545859713063976 1104 82-83 -0.33402115771023944 1104 84-85 -0.441500664045666 1104 86-87 -0.2830951671560946 1104 88-89 -0.5077030765165595 1104 90-91 -0.5596490375387795 1104 92-93 -0.673214252098358 1104 94-95 -0.9369815749906465 1104 96-97 -1.1324776429505796 1104 98-99 -1.2154426707549408 1104 100-101 -1.5471867601419547 1105 1 0.20238788187428014 1105 2 0.16616915597257886 1105 3 0.17162067256072433 1105 4 0.12005226793951351 1105 5 0.12203728582849038 1105 6 0.13877301863063707 1105 7 0.15081370202927502 1105 8 0.14726295405613854 1105 9 0.2691657414744384 1105 10-11 0.19887582626000722 1105 12-13 0.1809603379861997 1105 14-15 0.07496003796665462 1105 16-17 0.13694774845554036 1105 18-19 0.21084485640470518 1105 20-21 0.15905794540226736 1105 22-23 0.24933051023096908 1105 24-25 0.1444056610040363 1105 26-27 0.17125947125211383 1105 28-29 0.19676353736570462 1105 30-31 0.07505503433176841 1105 32-33 0.18990246443929237 1105 34-35 0.04840021366344871 1105 36-37 0.01787541037366225 1105 38-39 0.03693879874624173 1105 40-41 0.09018468083585418 1105 42-43 0.1855033980172962 1105 44-45 0.13073409921916124 1105 46-47 0.17995138486965345 1105 48-49 0.0352242055773857 1105 50-51 0.07187415624439808 1105 52-53 0.07543575295137117 1105 54-55 0.1784844308339757 1105 56-57 0.1905907950020449 1105 58-59 0.1452023673152354 1105 60-61 -0.036344953171116856 1105 62-63 -0.09877622116529494 1105 64-65 -0.09172205434666125 1105 66-67 0.07496917445728357 1105 68-69 -0.001039201175103699 1105 70-71 -0.2606280387914026 1105 72-73 -0.4050057237661022 1105 74-75 -0.48388517064036307 1105 76-77 -0.554954949257251 1105 78-79 -0.48888232568248924 1105 80-81 -0.393342420003826 1105 82-83 -0.5761413575084404 1105 84-85 -0.46410488604842826 1105 86-87 -0.4025919815797856 1105 88-89 -0.2636220626939725 1105 90-91 -0.45793636689563755 1105 92-93 -0.6395881499816518 1105 94-95 -0.7665900539358965 1105 96-97 -0.9544391343778678 1105 98-99 -0.8882688313422129 1105 100-101 -0.9621371314657061 1106 1 0.2779642561534459 1106 2 0.3898483599228335 1106 3 0.3944153329330966 1106 4 0.37026797658088384 1106 5 0.4528917130855348 1106 6 0.37559643752378946 1106 7 0.4503430111695579 1106 8 0.2510689386387952 1106 9 0.5510061526036623 1106 10-11 0.48595669430473265 1106 12-13 0.5398044909597459 1106 14-15 0.544394340574506 1106 16-17 0.09665039419208199 1106 18-19 0.479457768925279 1106 20-21 0.5691238223871622 1106 22-23 0.6337041657523557 1106 24-25 0.6341892024149658 1106 26-27 0.49619355479628524 1106 28-29 0.477053036913766 1106 30-31 0.41366096049743817 1106 32-33 0.397479797862097 1106 34-35 0.39645676366719584 1106 36-37 0.4129109539475948 1106 38-39 0.46539161716811606 1106 40-41 0.5038276109999273 1106 42-43 0.3417320097062202 1106 44-45 0.4695958956991433 1106 46-47 0.5994476568401694 1106 48-49 0.8286566372630659 1106 50-51 0.762619900291206 1106 52-53 0.702691911403484 1106 54-55 0.7910702307716093 1106 56-57 0.559874910715024 1106 58-59 0.7322270879597212 1106 60-61 1.0420789527933323 1106 62-63 0.7785536492516414 1106 64-65 0.6310883943983576 1106 66-67 0.4267311568453529 1106 68-69 0.45008927276026967 1106 70-71 0.3934273034018396 1106 72-73 0.42000789370531066 1106 74-75 0.5457993382030963 1106 76-77 0.25357560716447125 1106 78-79 0.4807720273692091 1106 80-81 0.36066101791432104 1106 82-83 0.4119017131721563 1106 84-85 0.6774662938561313 1106 86-87 0.7453372746694491 1106 88-89 0.2589127802709221 1106 90-91 0.2308453035931315 1106 92-93 0.30191454785652994 1106 94-95 0.031655364475714265 1106 96-97 -0.22704778798491887 1106 98-99 -0.5437681188919008 1106 100-101 -0.7737031998846202 1107 1 0.16881327826989434 1107 2 0.04172736544739308 1107 3 0.1528436565759641 1107 4 0.234702224585007 1107 5 0.13814421191307247 1107 6 0.09739234668406738 1107 7 0.23647883114190904 1107 8 0.1819692876827972 1107 9 0.26235038011700595 1107 10-11 0.21116070568933765 1107 12-13 0.2471156954111109 1107 14-15 0.2446210669352027 1107 16-17 0.3218132366531137 1107 18-19 0.31833197092830545 1107 20-21 0.2879426868707782 1107 22-23 0.396612875420999 1107 24-25 0.3388980264197059 1107 26-27 0.2178538857728114 1107 28-29 0.29102454441525083 1107 30-31 0.28869913408904324 1107 32-33 0.3490111827252278 1107 34-35 0.3968654800922238 1107 36-37 0.38068964999356325 1107 38-39 0.33168774550561153 1107 40-41 0.39490580979376233 1107 42-43 0.3101033760982901 1107 44-45 0.3279694835915521 1107 46-47 0.3712347106280447 1107 48-49 0.4145784328839568 1107 50-51 0.38999916228824816 1107 52-53 0.3269158501686249 1107 54-55 0.4015479830672959 1107 56-57 0.37339049716138817 1107 58-59 0.21304137863898376 1107 60-61 0.47423040470506095 1107 62-63 0.35659506428789456 1107 64-65 0.05150174840804311 1107 66-67 0.1314565565217336 1107 68-69 0.1913179948678696 1107 70-71 -0.05834064512277237 1107 72-73 0.12403720525820816 1107 74-75 -0.05746277199160943 1107 76-77 -0.1311764505159836 1107 78-79 -0.13244152151271749 1107 80-81 -0.07404506930289756 1107 82-83 -0.19760780369566433 1107 84-85 -0.2562221011738366 1107 86-87 -0.20347849268595652 1107 88-89 -0.16701991205236055 1107 90-91 -0.1426940329079578 1107 92-93 -0.3568330916867559 1107 94-95 -0.42901047665043635 1107 96-97 -0.5630172837043368 1107 98-99 -0.7457217779984369 1107 100-101 -0.8176287297160556 1108 1 0.07907319423227932 1108 2 0.015509943315695551 1108 3 -0.04758193612935813 1108 4 0.06267708633603064 1108 5 0.05219978494847055 1108 6 0.1719010146881601 1108 7 0.07702252523867514 1108 8 -0.0013258188483007416 1108 9 0.07856272189646063 1108 10-11 0.0019893260392009893 1108 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-0.8434287329060624 2316 54-55 -0.6990119830838495 2316 56-57 -0.7242460124864678 2316 58-59 -0.39392171554609234 2316 60-61 -0.6426043970117448 2316 62-63 -0.46995619683589496 2316 64-65 -0.49113723577149315 2316 66-67 -0.21482823767265913 2316 68-69 -0.3263227741205803 2316 70-71 -0.3666810067714472 2316 72-73 -0.4135551056639706 2316 74-75 -0.37259085270288494 2316 76-77 -0.5918086076501545 2316 78-79 -0.4863483573106997 2316 80-81 -0.5746260901490636 2316 82-83 -0.8918405919866714 2316 84-85 -0.9294284287147043 2316 86-87 -0.6239278450524104 2316 88-89 -0.6926608601267219 2316 90-91 -0.6221953512464751 2316 92-93 -0.6685137685992331 2316 94-95 -0.5044653407488333 2316 96-97 -0.44768514992393804 2316 98-99 -0.5685237303575512 2316 100-101 -0.358942720157545 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9222.0 3 3033.0 4 2798.0 5 3173.0 6 3673.0 7 4507.0 8 5516.0 9 6841.0 10 8598.0 11 10627.0 12 13197.0 13 15791.0 14 17989.0 15 21652.0 16 24845.0 17 29531.0 18 29745.0 19 27334.0 20 28262.0 21 30206.0 22 33497.0 23 36689.0 24 41021.0 25 45613.0 26 51430.0 27 58237.0 28 66049.0 29 76104.0 30 88668.0 31 103762.0 32 124721.0 33 154064.0 34 193794.0 35 248892.0 36 308432.0 37 318726.0 38 161378.0 39 10185.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.425290517547026 18.531821902057395 14.461138411274103 23.581749169121473 2 15.296094217157139 19.374478853073292 40.83404198970017 24.4953849400694 3 19.38905273971468 23.27373949927924 29.74549732407668 27.5917104369294 4 12.743318927317816 16.211032942786197 37.06738470356131 33.978263426334685 5 13.688129889935022 37.09720196260443 34.61999403262167 14.594674114838883 6 32.81641881845111 35.90543199521267 16.989676076113245 14.288473110222972 7 28.612910616166086 32.656400891381615 20.380059334297517 18.35062915815478 8 26.20374639602464 35.21130491431996 19.36635428764421 19.218594402011192 9 25.903420379828713 16.929592302385462 20.291294218589925 36.8756930991959 10-11 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26.689051145657256 28.71760574254827 22.03759615452287 36-37 22.544688781616216 26.223738445661233 28.816745465301075 22.41482730742148 38-39 21.980279350011017 26.727636325640052 28.74050409631275 22.55158022803618 40-41 22.522717702478023 26.520764337009346 28.782240100482444 22.174277860030188 42-43 22.193125420069993 26.50879622425364 28.5696844384554 22.728393917220966 44-45 22.26348534026074 26.716923034274227 28.731715279382524 22.287876346082513 46-47 22.75953363200494 26.86842345379718 28.08534506998396 22.286697844213922 48-49 22.36279107102884 26.984033065114428 28.049116044433838 22.6040598194229 50-51 22.345439027544288 27.243450148713304 28.114356653304025 22.296754170438383 52-53 22.758098844072805 27.050363640989673 27.894022320910512 22.297515194027017 54-55 22.344678847224948 27.121914825472416 28.06059492567774 22.472811401624902 56-57 22.449285231425822 27.207251858183163 27.920655185909332 22.422807724481682 58-59 22.521039908168426 26.890364057240802 27.837294411399828 22.751301623190948 60-61 22.159765266112352 27.11676637229766 28.20739433261567 22.516074028974316 62-63 22.18280943686086 27.072514444438696 28.324983059789886 22.41969305891055 64-65 21.942497242287303 27.265275825058815 28.132765764476027 22.65946116817786 66-67 21.972659825865115 27.156676091197905 27.936284183234072 22.934379899702904 68-69 22.400079295905336 26.80031917015759 28.154371248860844 22.645230285076227 70-71 22.341491352907557 26.83060918850045 27.83508802277034 22.992811435821654 72-73 22.279993006537232 26.889491908377362 27.949104971255416 22.881410113829986 74-75 22.17091517054049 27.385517563418254 27.718304217690676 22.72526304835058 76-77 22.324983178271435 26.929224218904004 27.755759050379552 22.99003355244501 78-79 22.45292147383954 27.0767845223392 27.718791220406374 22.75150278341489 80-81 22.63117384344217 27.107912990470105 27.43687022520218 22.82404294088554 82-83 22.521856330899865 27.109358807874855 27.652266220100998 22.71651864112428 84-85 22.50483796639113 27.234337937019976 27.51923810406323 22.74158599252566 86-87 22.608338252894544 27.19606523358849 27.301268688299494 22.89432782521747 88-89 22.537016278243467 27.227872852439187 27.07324992276096 23.16186094655638 90-91 22.49417893660087 27.192931789165193 27.17158737246918 23.141301901764756 92-93 22.77608088882671 26.801853916143287 27.38649068509379 23.035574509936218 94-95 22.498394532864424 26.636722781614868 27.510245912241636 23.354636773279065 96-97 22.644993067483394 26.393518614324442 27.564865591954142 23.39662272623802 98-99 22.579422343080687 26.566565290676238 27.084191180578514 23.76982118566456 100-101 23.390880060929636 25.419300224448182 27.176597585882647 24.013222128739535 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 478.0 1 352.5 2 298.0 3 370.5 4 329.0 5 335.5 6 615.5 7 1298.0 8 2087.0 9 2459.0 10 2165.0 11 1783.5 12 1760.5 13 1902.0 14 2076.0 15 2247.0 16 2432.0 17 2636.0 18 2965.0 19 3452.0 20 4057.5 21 4902.5 22 6091.5 23 7752.5 24 9780.0 25 12312.5 26 15641.5 27 19671.0 28 23138.5 29 26022.5 30 28986.0 31 33866.5 32 40722.0 33 44204.5 34 45403.0 35 48386.0 36 52631.0 37 57118.5 38 60950.5 39 63837.0 40 67756.0 41 72602.0 42 75216.0 43 77124.0 44 80187.5 45 84219.0 46 86937.5 47 90293.5 48 94246.5 49 93432.0 50 89935.5 51 86477.0 52 83820.5 53 83038.0 54 82375.0 55 78066.0 56 71834.5 57 66061.5 58 61282.0 59 54435.0 60 44272.5 61 36502.5 62 30626.0 63 23289.0 64 16888.5 65 12832.5 66 9665.0 67 7326.5 68 5589.5 69 4146.5 70 3043.0 71 2163.0 72 1456.0 73 1022.5 74 763.0 75 529.0 76 362.0 77 245.0 78 141.5 79 94.5 80 68.0 81 40.5 82 26.0 83 19.5 84 14.0 85 9.5 86 10.5 87 10.5 88 6.5 89 5.5 90 4.0 91 3.0 92 3.0 93 2.0 94 1.0 95 1.0 96 0.5 97 0.0 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1443459803573659 2 0.05198936885650686 3 0.15228707727100896 4 0.12697483085877173 5 0.19364695702956652 6 0.19712118692928535 7 0.16630807650916 8 0.07113899318471902 9 0.12093628841402232 10-11 0.10207618324412006 12-13 0.15073608178006306 14-15 0.13698392176034266 16-17 0.13464708855398416 18-19 0.13338561222134815 20-21 0.13355105174038237 22-23 0.1964801087930277 24-25 0.21546429360220562 26-27 0.1471584521809478 28-29 0.12852582634971765 30-31 0.10073198715196696 32-33 0.12416235903518981 34-35 0.09467276476733827 36-37 0.08896510136065731 38-39 0.05374716374624555 40-41 0.060364744507614775 42-43 0.06361149506866154 44-45 0.03836128847606214 46-47 0.05014885420725105 48-49 0.04115308035976478 50-51 0.0512655709607321 52-53 0.026945961662700253 54-55 0.04814290003896101 56-57 0.027132081121613763 58-59 0.04979729522930331 60-61 0.038774887273647723 62-63 0.05023157396676816 64-65 0.039043726492078344 66-67 0.03650009388692705 68-69 0.06352877530914443 70-71 0.054801840680088774 72-73 0.05209276855590325 74-75 0.04502022911718991 76-77 0.022396374889258924 78-79 0.02223093537022469 80-81 0.025581085630667855 82-83 0.022292975189862528 84-85 0.01919098420797071 86-87 0.024030090139721946 88-89 0.06623784743332994 90-91 0.10962436130005683 92-93 0.08036224637087734 94-95 0.07604013893610809 96-97 0.0683265213611371 98-99 0.05728343346560223 100-101 0.13290997360412474 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2417802.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.987241088343055 #Duplication Level Percentage of deduplicated Percentage of total 1 76.94420282984996 44.617820398448764 2 13.218587322672464 15.330188198542466 3 4.309749263859341 7.497314087811617 4 1.9971808583694777 4.632440317251793 5 1.0205393407299888 2.9589130395524266 6 0.5905058082732525 2.0545081601044783 7 0.3997114032589244 1.6224713054574613 8 0.2808947362808222 1.3030648634530062 9 0.20637050701013096 1.0770170709168048 >10 0.9439002821114003 9.677504715534875 >50 0.054239547831609204 2.142082432059363 >100 0.028617646601052295 3.0937592300586485 >500 0.0030716816301102567 1.2197792681736568 >1k 0.002285902608451857 2.1764404628088068 >5k 1.4286891302824105E-4 0.5966964498260603 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7883 0.3260399321367093 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6522 0.26974913578531245 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3964 0.16395056336292219 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2931 0.12122580757233223 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2816 0.1164694214000981 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2609 0.10790792629007669 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2555 0.10567449278311458 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.271975951711513E-5 2 0.0 0.0 0.0 0.0 8.271975951711513E-5 3 0.0 0.0 0.0 0.0 8.271975951711513E-5 4 0.0 0.0 0.0 0.0 8.271975951711513E-5 5 0.0 0.0 0.0 4.1359879758557563E-5 8.271975951711513E-5 6 0.0 0.0 0.0 4.1359879758557563E-5 8.271975951711513E-5 7 0.0 0.0 0.0 4.1359879758557563E-5 8.271975951711513E-5 8 0.0 0.0 0.0 4.1359879758557563E-5 1.240796392756727E-4 9 0.0 0.0 0.0 4.1359879758557563E-5 1.240796392756727E-4 10-11 0.0 0.0 0.0 4.1359879758557563E-5 1.240796392756727E-4 12-13 0.0 0.0 0.0 4.1359879758557563E-5 2.2747933867206662E-4 14-15 0.0 0.0 0.0 4.1359879758557563E-5 3.1019909818918176E-4 16-17 0.0 0.0 0.0 4.1359879758557563E-5 3.9291885770629685E-4 18-19 0.0 0.0 0.0 6.203981963783634E-5 4.1359879758557565E-4 20-21 0.0 0.0 0.0 8.271975951711513E-5 4.1359879758557565E-4 22-23 0.0 0.0 0.0 1.8611945891350903E-4 4.1359879758557565E-4 24-25 0.0 0.0 0.0 3.3087903806846056E-4 4.1359879758557565E-4 26-27 0.0 0.0 0.0 7.237978957747574E-4 4.1359879758557565E-4 28-29 0.0 0.0 0.0 0.003039951162253981 4.1359879758557565E-4 30-31 0.0 0.0 0.0 0.011787565731188906 4.1359879758557565E-4 32-33 0.0 0.0 0.0 0.031867787353968606 4.1359879758557565E-4 34-35 0.0 0.0 0.0 0.061274661862303036 4.963185571026908E-4 36-37 0.0 0.0 0.0 0.10968640111969466 4.963185571026908E-4 38-39 0.0 0.0 0.0 0.18390670534642622 4.963185571026908E-4 40-41 0.0 0.0 0.0 0.2593471260260352 4.963185571026908E-4 42-43 0.0 0.0 0.0 0.3339396691705938 4.963185571026908E-4 44-45 0.0 0.0 0.0 0.42451780584183485 5.376784368612483E-4 46-47 0.0 0.0 0.0 0.5313296953183098 5.790383166198059E-4 48-49 0.0 4.1359879758557563E-5 0.0 0.6408299769790908 5.790383166198059E-4 50-51 0.0 4.1359879758557563E-5 0.0 0.7563688010846215 5.790383166198059E-4 52-53 0.0 4.1359879758557563E-5 0.0 0.8691158333064495 5.790383166198059E-4 54-55 0.0 4.1359879758557563E-5 0.0 0.991334277992987 5.790383166198059E-4 56-57 0.0 4.1359879758557563E-5 0.0 1.1288351982503118 6.203981963783635E-4 58-59 0.0 8.271975951711513E-5 0.0 1.2736154573451426 6.203981963783635E-4 60-61 0.0 8.271975951711513E-5 0.0 1.4260266142554272 6.203981963783635E-4 62-63 0.0 8.271975951711513E-5 0.0 1.5754391798832161 6.203981963783635E-4 64-65 0.0 8.271975951711513E-5 0.0 1.7304146493385315 6.203981963783635E-4 66-67 0.0 8.271975951711513E-5 0.0 1.912935798713046 6.203981963783635E-4 68-69 0.0 8.271975951711513E-5 0.0 2.1076167527365763 6.410781362576423E-4 70-71 0.0 8.271975951711513E-5 0.0 2.309411606078579 6.824380160161998E-4 72-73 2.0679939879278782E-5 8.271975951711513E-5 0.0 2.512943574370441 7.031179558954786E-4 74-75 4.1359879758557563E-5 8.271975951711513E-5 0.0 2.7196188935239527 7.031179558954786E-4 76-77 4.1359879758557563E-5 8.271975951711513E-5 0.0 2.9373373005729997 7.031179558954786E-4 78-79 4.1359879758557563E-5 8.271975951711513E-5 0.0 3.184938220747605 7.444778356540362E-4 80-81 4.1359879758557563E-5 8.271975951711513E-5 0.0 3.435703171723739 7.444778356540362E-4 82-83 4.1359879758557563E-5 8.271975951711513E-5 0.0 3.694781458531344 7.444778356540362E-4 84-85 4.1359879758557563E-5 8.271975951711513E-5 0.0 3.9541906243770164 7.444778356540362E-4 86-87 4.1359879758557563E-5 8.271975951711513E-5 0.0 4.226359313128205 7.651577755333149E-4 88-89 4.1359879758557563E-5 8.271975951711513E-5 0.0 4.520303978572274 7.858377154125937E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7440 0.0 32.13251 1 GTATCAA 10555 0.0 28.063774 1 ATCAACG 12005 0.0 24.011023 3 TCAACGC 12070 0.0 23.881716 4 TATCAAC 12200 0.0 23.743715 2 CAACGCA 12160 0.0 23.665842 5 GTGTAGC 1715 0.0 23.603083 1 AACGCAG 12510 0.0 23.15006 6 GAGTACT 7670 0.0 22.900484 12-13 TAAGGTG 1300 0.0 21.953718 5 TACATGG 6430 0.0 21.822386 2 GTACTTT 8110 0.0 21.539024 14-15 GTACATG 6915 0.0 21.28042 1 GTGTAAG 915 0.0 20.81866 1 ACGCAGA 13785 0.0 20.795845 7 CGCAGAG 13750 0.0 20.744627 8 TAGCCCT 1720 0.0 20.74116 4 TAGGACG 115 0.0036944295 20.68104 4 AGAGTAC 10325 0.0 19.935535 10-11 ACATGGG 6725 0.0 19.804596 3 >>END_MODULE