FastQCFastQC Report
Thu 26 May 2016
SRR938088_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938088_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2417802
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78930.32645353093429486No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67050.2773179937811285No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42320.17503501113821562No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT28810.11915781358440435No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA28700.1187028549070602No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA26560.1098518406387289No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA24940.10315154011784258No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC24930.10311018023808402No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA24740.10232434252267142No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75200.029.6301611
GTATCAA107050.027.9597241
TCAACGC126200.023.332844
ATCAACG127150.023.1245043
CAACGCA127800.023.0778855
TATCAAC128500.022.9204252
AACGCAG131500.022.4271536
GGACCGA5500.021.5866686
TACATGG62300.021.3503062
GAGTACT83750.021.265812-13
GTACATG66700.021.1548331
AGAGTAC112050.021.15022510-11
TAAGGTG14900.020.3999465
GTACTTT87700.020.17218614-15
AGTACTT88150.020.15044212-13
ACGCAGA145850.020.0903287
CGCAGAG146400.019.852668
ACATGGG64500.019.7366393
GTAAGGT13850.019.5461124
GCAGAGT157550.018.327099
Produced by FastQC (version 0.11.5)