##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938088_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2417802 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.4811051525311 33.0 31.0 34.0 31.0 34.0 2 32.69660129324072 34.0 31.0 34.0 31.0 34.0 3 32.75819814856634 34.0 31.0 34.0 31.0 34.0 4 36.222554617789214 37.0 35.0 37.0 35.0 37.0 5 36.19243387175625 37.0 35.0 37.0 35.0 37.0 6 36.22162443409344 37.0 37.0 37.0 35.0 37.0 7 36.17454696455707 37.0 37.0 37.0 35.0 37.0 8 36.15879712234501 37.0 37.0 37.0 35.0 37.0 9 37.84281343137279 39.0 38.0 39.0 35.0 39.0 10-11 37.81791705855153 39.0 38.0 39.0 35.0 39.0 12-13 37.74252254733845 39.0 38.0 39.0 35.0 39.0 14-15 39.020818495476476 40.0 38.0 41.0 35.5 41.0 16-17 38.906000780874535 40.0 38.0 41.0 35.0 41.0 18-19 39.14741715822883 40.0 38.5 41.0 36.0 41.0 20-21 39.12164685114828 40.0 39.0 41.0 36.0 41.0 22-23 39.03375855425713 40.0 39.0 41.0 35.0 41.0 24-25 38.93652354493875 40.0 38.0 41.0 35.0 41.0 26-27 38.77029756779091 40.0 38.0 41.0 35.0 41.0 28-29 38.65645470555488 40.0 38.0 41.0 34.5 41.0 30-31 38.423738172108386 40.0 38.0 41.0 34.0 41.0 32-33 38.219359980676664 40.0 38.0 41.0 34.0 41.0 34-35 38.07569519753892 40.0 38.0 41.0 33.0 41.0 36-37 38.075951628793426 40.0 38.0 41.0 33.5 41.0 38-39 37.929890247423074 40.0 38.0 41.0 33.0 41.0 40-41 37.76196024322918 40.0 37.0 41.0 33.0 41.0 42-43 37.538230177657226 40.0 37.0 41.0 33.0 41.0 44-45 37.2859568318663 40.0 37.0 41.0 32.0 41.0 46-47 36.92832953236038 40.0 36.0 41.0 31.0 41.0 48-49 36.54886256194676 39.0 36.0 41.0 30.0 41.0 50-51 36.17066016985675 39.0 35.0 40.5 29.5 41.0 52-53 35.649515551728385 39.0 35.0 40.0 28.0 41.0 54-55 35.33910675894883 38.0 34.5 40.0 27.5 41.0 56-57 34.99550004508227 38.0 34.0 40.0 26.5 41.0 58-59 34.55528637167146 38.0 34.0 40.0 26.0 41.0 60-61 34.066884509153354 37.0 33.0 40.0 25.0 41.0 62-63 33.60341003936634 36.5 33.0 39.5 23.0 41.0 64-65 33.10991057166798 36.0 32.0 39.0 22.0 40.0 66-67 32.553637766864284 35.5 31.5 39.0 20.5 40.0 68-69 31.968784251150424 35.0 31.0 38.0 18.5 40.0 70-71 31.344365047261935 35.0 30.5 37.5 15.0 40.0 72-73 30.749454049587186 34.5 29.5 37.0 10.0 39.0 74-75 30.142777820516322 34.0 29.0 36.0 7.5 39.0 76-77 28.801441970847904 32.5 27.5 35.0 7.0 37.0 78-79 29.475594362152073 34.0 29.0 35.0 3.5 37.5 80-81 29.137354299483583 34.0 29.0 35.0 2.0 37.0 82-83 28.553523820395547 34.0 27.5 35.0 2.0 36.5 84-85 27.938444711353533 33.0 26.5 35.0 2.0 36.0 86-87 27.28640889535206 33.0 26.0 35.0 2.0 36.0 88-89 26.527499977252063 32.0 25.0 35.0 2.0 35.0 90-91 25.961727014867222 32.0 23.5 34.0 2.0 35.0 92-93 25.136620161617866 31.5 19.5 34.0 2.0 35.0 94-95 24.292588474986786 31.0 14.0 34.0 2.0 35.0 96-97 23.220594986686255 31.0 2.0 34.0 2.0 35.0 98-99 21.75451050168707 30.0 2.0 34.0 2.0 35.0 100-101 17.396704113901798 23.0 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.0951669092365961 1101 2 0.10430693090474819 1101 3 0.1082108695189703 1101 4 0.06710148087324797 1101 5 0.09619435454654024 1101 6 0.012345388359108256 1101 7 0.07677180945399442 1101 8 0.04826902520467513 1101 9 0.03101755949142415 1101 10-11 0.06949564449275414 1101 12-13 0.1733563824125568 1101 14-15 0.17502554927052216 1101 16-17 0.1705704632536751 1101 18-19 0.18105857324072616 1101 20-21 0.20440473654679892 1101 22-23 0.24303906343708803 1101 24-25 0.25616239088993353 1101 26-27 0.24387365970361685 1101 28-29 0.2309076084868451 1101 30-31 0.244579674735796 1101 32-33 0.2469433979510356 1101 34-35 0.16611012663258862 1101 36-37 0.09877696423993143 1101 38-39 0.1265841520416089 1101 40-41 0.045991459421266256 1101 42-43 0.16896170805601685 1101 44-45 0.07451711900145597 1101 46-47 -0.030516437986193523 1101 48-49 0.1486106418147628 1101 50-51 0.02967510953340735 1101 52-53 -0.15037753068952497 1101 54-55 -0.2244042717492789 1101 56-57 -0.016986631278840036 1101 58-59 0.06918312635513502 1101 60-61 -0.05562582660166271 1101 62-63 -0.16197373833960427 1101 64-65 -0.005031012148194236 1101 66-67 -0.24365305249440183 1101 68-69 -0.10177420275664417 1101 70-71 -0.17452623386933297 1101 72-73 -0.17133316697321632 1101 74-75 -0.44934486117113437 1101 76-77 -0.3483588737227379 1101 78-79 -0.43715718079079124 1101 80-81 -0.46951062132382404 1101 82-83 -0.5445253394663538 1101 84-85 -0.5514402723600718 1101 86-87 -0.5745101613151071 1101 88-89 -0.5364524337069163 1101 90-91 -0.9909073286176735 1101 92-93 -0.984696685527485 1101 94-95 -0.8533471998527133 1101 96-97 -0.884196367905691 1101 98-99 -0.730607117024693 1101 100-101 -1.2876693815936342 1102 1 0.14013503309816144 1102 2 0.07636977243480203 1102 3 0.08832623078187396 1102 4 0.06550767395157209 1102 5 0.056258106823406706 1102 6 0.05481654804338376 1102 7 0.020062641269419146 1102 8 0.102564714336431 1102 9 0.07662320612165985 1102 10-11 0.08280013751036819 1102 12-13 0.052667250657854936 1102 14-15 0.18929012121952127 1102 16-17 0.035643322998673455 1102 18-19 0.11074132976774109 1102 20-21 0.05824505227235477 1102 22-23 0.1960862704801869 1102 24-25 0.17570929435077431 1102 26-27 0.09304260323731484 1102 28-29 0.04029968498958425 1102 30-31 0.08549497528223782 1102 32-33 -0.04799436768345089 1102 34-35 -0.05785389886831638 1102 36-37 0.014779544689233148 1102 38-39 0.044734577056786407 1102 40-41 0.11688550920876395 1102 42-43 0.09361258844460707 1102 44-45 0.019804004247355067 1102 46-47 -0.11798987453749987 1102 48-49 -0.016712673303636905 1102 50-51 -0.01220026994443657 1102 52-53 -0.07347634671866388 1102 54-55 -0.07399823046208809 1102 56-57 -0.03540269078096969 1102 58-59 -0.057065355733513456 1102 60-61 -0.18311140644379975 1102 62-63 -0.10597039893462323 1102 64-65 -0.13323380510508542 1102 66-67 -0.3469431253541515 1102 68-69 -0.25731078442027666 1102 70-71 -0.34350012579404776 1102 72-73 -0.34803322768966893 1102 74-75 -0.4431024507279062 1102 76-77 -0.3706569915126572 1102 78-79 -0.380432833492673 1102 80-81 -0.4167745861082821 1102 82-83 -0.5647401239229453 1102 84-85 -0.694917048316352 1102 86-87 -0.9048532092811037 1102 88-89 -0.9107959370463199 1102 90-91 -1.1147347420967257 1102 92-93 -1.111309466341087 1102 94-95 -1.090555001917071 1102 96-97 -0.8233888390653732 1102 98-99 -1.0448830733088492 1102 100-101 -1.3921775783149464 1103 1 0.1789467811262213 1103 2 0.18082516199614673 1103 3 0.15408013124189068 1103 4 0.10758559025980219 1103 5 0.10008077977443008 1103 6 0.0784002458773827 1103 7 0.19155788155714504 1103 8 0.11811586761249515 1103 9 0.2487343616594444 1103 10-11 0.16541931206276672 1103 12-13 0.2114979607980345 1103 14-15 0.20240760150014125 1103 16-17 0.25703264605731846 1103 18-19 0.253889525035099 1103 20-21 0.33948316308863724 1103 22-23 0.3530194568016256 1103 24-25 0.3715710506583534 1103 26-27 0.34909200165976273 1103 28-29 0.37017066589015 1103 30-31 0.35297152001788135 1103 32-33 0.4086529733801214 1103 34-35 0.4986566282339737 1103 36-37 0.39750766556209527 1103 38-39 0.3615132191353112 1103 40-41 0.38493475415724987 1103 42-43 0.31507117672646245 1103 44-45 0.2550757541332658 1103 46-47 0.3545635283437676 1103 48-49 0.3516871384888205 1103 50-51 0.25821610895854974 1103 52-53 0.1932554454129729 1103 54-55 0.18052561656475774 1103 56-57 0.3440757198749793 1103 58-59 0.4428902175927121 1103 60-61 0.18340420953203562 1103 62-63 0.10119966233380495 1103 64-65 0.07260921122374242 1103 66-67 0.02878012070096503 1103 68-69 0.09723366042804571 1103 70-71 -0.00347835672389607 1103 72-73 -0.16702381327361593 1103 74-75 -0.12133560191187343 1103 76-77 -0.1777914104723486 1103 78-79 -0.24899712494780601 1103 80-81 -0.3301562031584311 1103 82-83 -0.2959445108550369 1103 84-85 -0.3246371804655155 1103 86-87 -0.3451761710055088 1103 88-89 -0.7331578003817931 1103 90-91 -0.8310757814385674 1103 92-93 -0.5738499047111922 1103 94-95 -0.5597316746823076 1103 96-97 -0.7090280177373351 1103 98-99 -0.9175835890621435 1103 100-101 -1.5884823897237172 1104 1 0.10947122734341974 1104 2 0.12738299275360987 1104 3 0.08729995011420755 1104 4 0.08769616337926323 1104 5 0.055654355489359375 1104 6 0.07649865915217191 1104 7 0.0656693408419855 1104 8 0.08539209439180695 1104 9 0.05550195585214368 1104 10-11 0.09551958421526052 1104 12-13 0.10186641556832399 1104 14-15 0.2747288562049519 1104 16-17 0.12462458101165197 1104 18-19 0.11871374538359447 1104 20-21 0.17355180045414187 1104 22-23 0.14140514005676863 1104 24-25 0.23516141271617386 1104 26-27 0.17929857602850774 1104 28-29 0.010178869034625393 1104 30-31 0.05748390798898839 1104 32-33 0.12113473502420646 1104 34-35 0.17903822496852229 1104 36-37 0.13841313467001015 1104 38-39 0.24325661311107893 1104 40-41 0.21075029441238513 1104 42-43 0.09955340497143084 1104 44-45 -0.059947006770478595 1104 46-47 -0.10235397078840691 1104 48-49 -0.1510446403235406 1104 50-51 0.0029368869026242805 1104 52-53 -0.058790119667975205 1104 54-55 -0.039624203709237804 1104 56-57 0.12752371222121894 1104 58-59 0.15471902022524375 1104 60-61 -0.22828282545456346 1104 62-63 -0.20040869960548235 1104 64-65 -0.1974432907823669 1104 66-67 -0.17446228504048378 1104 68-69 -0.29477485880089915 1104 70-71 -0.48727234867314806 1104 72-73 -0.6522595643081814 1104 74-75 -0.49668562312528053 1104 76-77 -0.5821416170090892 1104 78-79 -0.5210605262236214 1104 80-81 -0.5706132274301403 1104 82-83 -0.4838804322553685 1104 84-85 -0.5176847333038275 1104 86-87 -0.7316292235253883 1104 88-89 -0.9077871301980629 1104 90-91 -1.011074502049059 1104 92-93 -0.9847993152042562 1104 94-95 -1.0665294662302465 1104 96-97 -1.414913773898867 1104 98-99 -1.4062429299695864 1104 100-101 -1.618315683389703 1105 1 0.013292492803358869 1105 2 0.08020562371520157 1105 3 0.010934181065813675 1105 4 0.004006242778011426 1105 5 0.07670322476128888 1105 6 0.03782002265727158 1105 7 0.08716707867279894 1105 8 0.09639383068242324 1105 9 0.09293399273138903 1105 10-11 0.11748428439316427 1105 12-13 0.1273791026117479 1105 14-15 0.13410001238910496 1105 16-17 0.05913255475694967 1105 18-19 0.13804192213584798 1105 20-21 0.11357062512444571 1105 22-23 0.1363883475951937 1105 24-25 0.05116122375641652 1105 26-27 0.05417361301827128 1105 28-29 0.018483472165648607 1105 30-31 0.051360720487430456 1105 32-33 0.08013278481688957 1105 34-35 -0.04207731501072942 1105 36-37 0.044355473421376246 1105 38-39 0.1390822068190758 1105 40-41 0.055565844730942615 1105 42-43 0.03674104201181905 1105 44-45 -0.04185776428519006 1105 46-47 -0.0802149284935112 1105 48-49 0.09409383509207458 1105 50-51 0.006042600974424772 1105 52-53 -0.034195224433688054 1105 54-55 0.0035621926142468396 1105 56-57 -0.09421780152472081 1105 58-59 0.13275494397106513 1105 60-61 -0.1823162249832393 1105 62-63 -0.366218213907608 1105 64-65 -0.20161520137943967 1105 66-67 -0.21862387415639972 1105 68-69 -0.32216208012981795 1105 70-71 -0.30826417348962565 1105 72-73 -0.30568579691408004 1105 74-75 -0.4417137531723796 1105 76-77 -0.3248139001777659 1105 78-79 -0.46555742977927395 1105 80-81 -0.5994145273306692 1105 82-83 -0.45575510915200823 1105 84-85 -0.7557878447223452 1105 86-87 -0.8580457977919203 1105 88-89 -1.1272884903517983 1105 90-91 -1.0334551810919983 1105 92-93 -0.9574694057266768 1105 94-95 -0.9384526309140249 1105 96-97 -1.3205253439405453 1105 98-99 -1.5051052496110628 1105 100-101 -1.928915880193074 1106 1 0.17562656687450584 1106 2 0.21058857069390058 1106 3 0.24173295430925634 1106 4 0.16294068550766383 1106 5 0.14373493176066887 1106 6 0.14645547307980422 1106 7 0.16329565128549461 1106 8 0.09227464425726595 1106 9 0.17823521514613816 1106 10-11 0.20397170698080203 1106 12-13 0.1307898844914277 1106 14-15 0.2413050362492868 1106 16-17 0.2096837663171911 1106 18-19 0.23798301177038184 1106 20-21 0.34072938905274697 1106 22-23 0.3591193610903005 1106 24-25 0.31046442366484683 1106 26-27 0.33173409235326545 1106 28-29 0.303500701227307 1106 30-31 0.47468705430888036 1106 32-33 0.4201687428356067 1106 34-35 0.4949170130163907 1106 36-37 0.5191583161178812 1106 38-39 0.4769180551086052 1106 40-41 0.4173749554113826 1106 42-43 0.5077875348221923 1106 44-45 0.32965320271316045 1106 46-47 0.35789415732024565 1106 48-49 0.30026047741833395 1106 50-51 0.4233877071709671 1106 52-53 0.3213603091935795 1106 54-55 0.10807391745448314 1106 56-57 0.2769266778734334 1106 58-59 0.4519005167140193 1106 60-61 0.2612444872302504 1106 62-63 0.29153229822773596 1106 64-65 0.25457226186325244 1106 66-67 0.1529999797064221 1106 68-69 0.009764969694337822 1106 70-71 -0.3189854652942792 1106 72-73 -0.16906249564430098 1106 74-75 -0.11330366203399933 1106 76-77 0.007330634479689735 1106 78-79 0.1219398565941745 1106 80-81 0.07835929238979489 1106 82-83 0.12241716962329008 1106 84-85 -0.06426156300252117 1106 86-87 0.019860348226846014 1106 88-89 -0.1498232346393671 1106 90-91 -0.3043607626764384 1106 92-93 -0.37391447600466776 1106 94-95 -0.615309540892671 1106 96-97 -0.5648502133904394 1106 98-99 -0.5736587583795334 1106 100-101 -1.1062853202189693 1107 1 0.06415265274765858 1107 2 0.04200191875796122 1107 3 0.07514817506387317 1107 4 -0.0017036591344208318 1107 5 0.034108304169350845 1107 6 0.09081148391381078 1107 7 0.025228954746587817 1107 8 0.05654055910911637 1107 9 0.06881163953234193 1107 10-11 0.1004310534344981 1107 12-13 6.014064930255358E-4 1107 14-15 0.13885450336847072 1107 16-17 0.10087988660107072 1107 18-19 0.11808442245011719 1107 20-21 0.06255799913043347 1107 22-23 0.052314038930411755 1107 24-25 0.12447926213402383 1107 26-27 0.09170242201022205 1107 28-29 0.12274768648199341 1107 30-31 -0.00726312030320031 1107 32-33 -0.05353980965242755 1107 34-35 0.08774978268679945 1107 36-37 0.05930657469042444 1107 38-39 -0.04738683419203227 1107 40-41 -0.11347291001145976 1107 42-43 -0.008797956096053383 1107 44-45 -0.15896804913561624 1107 46-47 -0.08137816709159296 1107 48-49 -0.09598563743340804 1107 50-51 -0.1211621129019207 1107 52-53 -0.08282302998628666 1107 54-55 -0.030536533182171866 1107 56-57 0.03847915527872203 1107 58-59 0.1318187121710963 1107 60-61 -0.003084325088643425 1107 62-63 -0.13903815891667648 1107 64-65 -0.3084118671113245 1107 66-67 -0.3604355930902372 1107 68-69 -0.07510535179982014 1107 70-71 -0.2674150375176332 1107 72-73 -0.5297900991643232 1107 74-75 -0.3885776425716365 1107 76-77 -0.30087560218883524 1107 78-79 -0.2593847305499857 1107 80-81 -0.22387573874274125 1107 82-83 -0.28392434002041966 1107 84-85 -0.25512825982970355 1107 86-87 -0.3304578235918818 1107 88-89 -0.542771691687566 1107 90-91 -0.8184493628843903 1107 92-93 -0.9118386217675223 1107 94-95 -1.0567528278627698 1107 96-97 -1.233577308109414 1107 98-99 -1.4140826955952086 1107 100-101 -1.6056594084444207 1108 1 -0.027860297330214223 1108 2 -0.013712659515832115 1108 3 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38-39 -0.3596678925897763 2316 40-41 -0.32062993326479017 2316 42-43 -0.43456382062723975 2316 44-45 -0.4231651452817502 2316 46-47 -0.43207254851312626 2316 48-49 -0.21314452818172924 2316 50-51 -0.045665905772118265 2316 52-53 0.060661288097719535 2316 54-55 0.0032471770181032866 2316 56-57 0.14047846394256425 2316 58-59 0.11132224891617426 2316 60-61 0.2658522958425422 2316 62-63 0.3082958609126507 2316 64-65 0.535403033885224 2316 66-67 0.5139683109826407 2316 68-69 0.6904189564909231 2316 70-71 0.8369412272826686 2316 72-73 0.8146844752747135 2316 74-75 1.0918435077972042 2316 76-77 0.8951625656977562 2316 78-79 0.9211177345030563 2316 80-81 1.0550461318015394 2316 82-83 1.238408560521961 2316 84-85 1.3461434114815027 2316 86-87 1.5760448100600506 2316 88-89 1.6358669085059567 2316 90-91 1.625091181722322 2316 92-93 1.5777193385407493 2316 94-95 2.178554161287611 2316 96-97 2.6127863403546208 2316 98-99 2.9960797038655933 2316 100-101 3.1006937449398393 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 7.0 4 19.0 5 37.0 6 80.0 7 194.0 8 536.0 9 1068.0 10 1807.0 11 3014.0 12 4673.0 13 6935.0 14 9506.0 15 13169.0 16 17020.0 17 22792.0 18 23316.0 19 19785.0 20 19204.0 21 20087.0 22 21571.0 23 24067.0 24 26982.0 25 30512.0 26 35652.0 27 42187.0 28 49907.0 29 59857.0 30 71999.0 31 88101.0 32 108811.0 33 139571.0 34 186240.0 35 260450.0 36 369921.0 37 460697.0 38 262756.0 39 15270.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.720036068604806 18.8738074507314 14.892073386374587 23.514083094289205 2 15.516919011622953 19.66710486800598 40.35180755984179 24.464168560529284 3 19.482434558742018 23.23832679513631 30.036093825746985 27.243144820374688 4 12.958014327895473 16.56054406462732 36.87400245098749 33.60743915648972 5 13.614895703521961 37.08796242489466 34.7460532862707 14.551088585312671 6 32.79044768316445 36.19029026466244 16.76349876062916 14.255763291543946 7 28.67364655997472 32.597913311346424 20.275481615119848 18.452958513559008 8 26.134149942799283 35.57780992819098 19.162735410095618 19.125304718914123 9 25.638286344373938 16.997049386178027 20.235197092235012 37.12946717721302 10-11 25.05236160777433 26.388120284456708 27.123705745962656 21.435812361806303 12-13 25.94284839811728 23.601258178990644 27.567334496935203 22.888558925956872 14-15 22.380637455010792 25.351352178548947 25.876560611662992 26.391449754777273 16-17 22.075464050222234 28.411644953497778 26.923557347499766 22.589333648780222 18-19 22.035882177283334 26.964635648411246 29.083026649824923 21.9164555244805 20-21 22.43086902897756 26.140395284642832 29.407329467011774 22.02140621936784 22-23 22.192636121568267 26.066712658852957 29.3418360974141 22.398815122164677 24-25 22.240115613319936 26.224911998501067 29.417286449661447 22.117685938517553 26-27 21.848332595951202 26.52776166154843 29.293389353691285 22.330516388809084 28-29 21.844764504506777 27.041823698475763 29.02437980234001 22.08903199467745 30-31 22.096033492725994 26.519810614881216 28.93551732025103 22.448638572141757 32-33 21.806624363781648 26.465380539845697 29.05792120281148 22.670073893561177 34-35 22.438490092150076 26.662614401686312 28.8482729409351 22.050622565228505 36-37 22.51389184728998 26.23140601831842 28.929040423362707 22.325661711028896 38-39 21.9705563082118 26.683337927856815 28.864430260820722 22.48167550311066 40-41 22.52215959041181 26.45086093635579 28.845323049923987 22.181656423308407 42-43 22.162703324351728 26.50212317847459 28.667097237706855 22.668076259466822 44-45 22.20420079977616 26.676817761921317 28.850879611084697 22.268101827217826 46-47 22.714471833898585 26.89686905451237 28.146993134254284 22.24166597733477 48-49 22.30849707966719 26.957226838812893 28.145336887268307 22.58893919425161 50-51 22.290631811072345 27.215439743873176 28.182781340264967 22.311147104789512 52-53 22.71790398215099 26.989596763419126 27.99991148293792 22.292587771491963 54-55 22.356605185771464 27.034318049316386 28.137648598247583 22.471428166664566 56-57 22.41108468034085 27.15677188117198 27.96653796919989 22.46560546928728 58-59 22.472829521237372 26.920942454618995 27.864170669265725 22.742057354877904 60-61 22.095911811164314 27.203428352568388 28.163423323038515 22.53723651322878 62-63 22.132875478672815 27.168139140161525 28.27893723775638 22.42004814340928 64-65 21.883296174744032 27.341544419768578 28.054228167596513 22.72093123789088 66-67 21.9657451983981 27.226943696599026 27.850335501481798 22.956975603521073 68-69 22.336489042038377 26.904047451885543 28.070010314008393 22.68945319206769 70-71 22.30860188832886 26.977199118815715 27.69461433916246 23.019584653692963 72-73 22.25473495208537 27.026488763737056 27.79009097750721 22.928685306670367 74-75 22.195060709049937 27.515086256821284 27.507764437634645 22.782088596494134 76-77 22.241621563370863 27.001869631325416 27.783995633183256 22.972513172120465 78-79 22.332436295488815 27.229187308534826 27.70136293213471 22.737013463841652 80-81 22.546444882262694 27.21310437911549 27.468446269621676 22.772004469000137 82-83 22.460829702814635 27.252142109465826 27.6318467859049 22.655181401814637 84-85 22.402344759970298 27.354595303447432 27.556493698561003 22.686566238021268 86-87 22.53688770362991 27.36591935913271 27.257111260831422 22.84008167640596 88-89 22.372005399392464 27.475841938287576 26.984551830154935 23.16760083216502 90-91 22.338671242805656 27.387103793848688 27.182795129523047 23.09142983382261 92-93 22.55441786116304 27.103377492018883 27.326770274032615 23.015434372785464 94-95 22.284130225077725 26.906720066714207 27.507036264269015 23.302113443939053 96-97 22.38763894470382 26.715749793119937 27.569020173417304 23.327591088758943 98-99 22.236256149810924 26.91038131930042 27.13684989042644 23.71651264046222 100-101 22.709857632043036 26.264951200035902 27.172886367260396 23.85230480066066 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 429.0 1 303.0 2 274.5 3 371.5 4 307.0 5 300.0 6 582.0 7 1310.5 8 2126.0 9 2459.5 10 2150.0 11 1755.0 12 1735.0 13 1862.0 14 1997.0 15 2163.0 16 2345.0 17 2582.5 18 2878.0 19 3250.0 20 3820.0 21 4658.0 22 5774.0 23 7337.5 24 9455.0 25 11932.5 26 15253.0 27 19407.0 28 22929.5 29 25941.5 30 29282.5 31 34298.5 32 41161.0 33 44634.5 34 45548.0 35 48389.5 36 52849.0 37 57309.0 38 60923.0 39 63841.5 40 67891.5 41 73378.0 42 75782.0 43 77510.5 44 80889.0 45 84548.0 46 87006.0 47 90914.0 48 95521.0 49 94293.0 50 90317.5 51 87297.0 52 85187.0 53 84817.5 54 84572.5 55 79656.0 56 72766.5 57 66606.0 58 61401.0 59 54397.0 60 43560.0 61 35596.5 62 30002.0 63 22004.0 64 15052.0 65 11226.5 66 8297.5 67 6259.0 68 4707.0 69 3406.0 70 2433.0 71 1573.0 72 1039.5 73 734.0 74 511.0 75 360.0 76 243.0 77 146.5 78 77.5 79 49.5 80 25.0 81 15.0 82 11.0 83 4.0 84 2.5 85 3.0 86 1.5 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.007734297514850264 2 0.014227798636943803 3 0.00885101426833132 4 4.1359879758557563E-5 5 0.0012821562725152844 6 0.0017784748296179754 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 9.099173546882664E-4 14-15 0.0 16-17 0.0028124718235819145 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.003618989478873787 26-27 0.019294383907367107 28-29 0.006845060100041277 30-31 0.003432870019960278 32-33 0.0 34-35 0.009264613065916895 36-37 7.031179558954786E-4 38-39 0.002833151763461193 40-41 0.0023368332063585023 42-43 0.003412190080080999 44-45 2.0679939879278782E-5 46-47 8.271975951711513E-5 48-49 5.169984969819695E-4 50-51 0.003867148757425132 52-53 0.00781701727436738 54-55 0.006927779859558393 56-57 0.015530634849338367 58-59 0.021445097654812097 60-61 0.003350150260443163 62-63 0.018239706973523888 64-65 0.019046224628815757 66-67 0.012470003747205107 68-69 0.028993275710748855 70-71 0.01544791508982125 72-73 0.004818425991871956 74-75 0.015303155510666301 76-77 0.01987342222398691 78-79 0.024278249418273293 80-81 0.01071220885746641 82-83 0.03275702476877759 84-85 0.02055586024000311 86-87 0.02905531553038669 88-89 0.02039042072096888 90-91 0.025891284728857034 92-93 0.022127535670828296 94-95 0.013483320801289765 96-97 0.014000319298271734 98-99 0.010257250180122275 100-101 0.009450732524830403 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2417802.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.52760833494488 #Duplication Level Percentage of deduplicated Percentage of total 1 74.854779850818 41.56506897554746 2 14.278468396474029 15.856984014845965 3 4.806841230627958 8.00737191547721 4 2.1294761079565956 4.729788611249465 5 1.1336149742472423 3.1473464146314747 6 0.6775529694050947 2.257373754678299 7 0.4329685580216936 1.6829195957822123 8 0.29907456897649987 1.3285516423255632 9 0.2136508358223396 1.0677167938778835 >10 1.0762470815151586 10.526303385487374 >50 0.058057103140989434 2.2021033078532946 >100 0.03345244642224629 3.4774698515019824 >500 0.0030570633263660866 1.1750524171448749 >1k 0.002609688205432114 2.3715514764812036 >5k 1.491250403104065E-4 0.6043978431157928 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7893 0.32645353093429486 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6705 0.2773179937811285 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4232 0.17503501113821562 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2881 0.11915781358440435 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2870 0.1187028549070602 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2656 0.1098518406387289 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2494 0.10315154011784258 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2493 0.10311018023808402 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2474 0.10232434252267142 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.1359879758557563E-5 2 0.0 0.0 0.0 0.0 4.1359879758557563E-5 3 0.0 0.0 0.0 0.0 4.1359879758557563E-5 4 0.0 0.0 0.0 0.0 4.1359879758557563E-5 5 0.0 1.240796392756727E-4 0.0 0.0 4.1359879758557563E-5 6 0.0 1.240796392756727E-4 0.0 0.0 4.1359879758557563E-5 7 0.0 1.240796392756727E-4 0.0 0.0 4.1359879758557563E-5 8 0.0 1.240796392756727E-4 0.0 0.0 4.1359879758557563E-5 9 0.0 1.240796392756727E-4 0.0 0.0 4.1359879758557563E-5 10-11 0.0 1.240796392756727E-4 0.0 0.0 4.1359879758557563E-5 12-13 0.0 1.240796392756727E-4 0.0 0.0 2.481592785513454E-4 14-15 0.0 1.240796392756727E-4 0.0 0.0 2.481592785513454E-4 16-17 0.0 1.240796392756727E-4 0.0 0.0 2.8951915830990296E-4 18-19 0.0 1.240796392756727E-4 0.0 2.0679939879278782E-5 3.722389178270181E-4 20-21 0.0 1.240796392756727E-4 0.0 4.1359879758557563E-5 3.722389178270181E-4 22-23 0.0 1.240796392756727E-4 0.0 1.4475957915495148E-4 3.722389178270181E-4 24-25 0.0 1.240796392756727E-4 0.0 2.8951915830990296E-4 3.9291885770629685E-4 26-27 0.0 1.240796392756727E-4 0.0 6.824380160161998E-4 4.7563861722341204E-4 28-29 0.0 1.240796392756727E-4 0.0 0.0029779113426161453 4.963185571026908E-4 30-31 0.0 1.6543951903423025E-4 0.0 0.012015045069860973 4.963185571026908E-4 32-33 0.0 1.6543951903423025E-4 0.0 0.03228138615155418 5.376784368612483E-4 34-35 0.0 1.6543951903423025E-4 0.0 0.061853700178922846 5.790383166198059E-4 36-37 0.0 1.8611945891350903E-4 0.0 0.11036883913571086 5.790383166198059E-4 38-39 0.0 2.0679939879278782E-4 0.0 0.1848993424606316 5.790383166198059E-4 40-41 0.0 2.0679939879278782E-4 0.0 0.26104288109613605 5.790383166198059E-4 42-43 0.0 2.0679939879278782E-4 0.0 0.3360697029781595 5.997182564990847E-4 44-45 0.0 2.0679939879278782E-4 0.0 0.4276818366433645 6.61758076136921E-4 46-47 0.0 2.0679939879278782E-4 0.0 0.535279563835252 7.031179558954786E-4 48-49 0.0 2.0679939879278782E-4 0.0 0.6457724826102385 7.031179558954786E-4 50-51 0.0 2.0679939879278782E-4 0.0 0.7620971444311817 7.031179558954786E-4 52-53 0.0 2.2747933867206662E-4 0.0 0.8751957356309574 7.031179558954786E-4 54-55 0.0 2.481592785513454E-4 0.0 0.9984068174317003 7.237978957747574E-4 56-57 0.0 2.481592785513454E-4 0.0 1.1369003748032305 7.651577755333149E-4 58-59 0.0 2.8951915830990296E-4 0.0 1.2832729892687658 7.858377154125937E-4 60-61 0.0 2.8951915830990296E-4 0.0 1.4373178614295132 7.858377154125937E-4 62-63 0.0 2.8951915830990296E-4 0.0 1.5882814225482482 7.858377154125937E-4 64-65 0.0 2.8951915830990296E-4 0.0 1.7446631279153544 7.858377154125937E-4 66-67 0.0 2.8951915830990296E-4 0.0 1.9287973126004527 7.858377154125937E-4 68-69 0.0 2.8951915830990296E-4 0.0 2.1246777031369817 7.858377154125937E-4 70-71 0.0 3.1019909818918176E-4 0.0 2.3283751109478774 7.858377154125937E-4 72-73 0.0 3.308790380684605E-4 0.0 2.532920396293824 9.926371142053815E-4 74-75 0.0 3.308790380684605E-4 0.0 2.742139348052487 0.001033996993963939 76-77 0.0 3.308790380684605E-4 0.0 2.962422067646565 0.0010546769338432179 78-79 0.0 3.308790380684605E-4 0.0 3.2128147797048725 0.0011167167534810543 80-81 0.0 3.308790380684605E-4 0.0 3.467984557875293 0.0011167167534810543 82-83 0.0 3.308790380684605E-4 0.0 3.732873907788975 0.0011580766332396119 84-85 0.0 3.308790380684605E-4 0.0 3.9985490954180696 0.0011580766332396119 86-87 0.0 3.308790380684605E-4 0.0 4.276652926914611 0.0011787565731188907 88-89 0.0 3.308790380684605E-4 0.0 4.578538689272323 0.0012201164528774483 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7520 0.0 29.630161 1 GTATCAA 10705 0.0 27.959724 1 TCAACGC 12620 0.0 23.33284 4 ATCAACG 12715 0.0 23.124504 3 CAACGCA 12780 0.0 23.077885 5 TATCAAC 12850 0.0 22.920425 2 AACGCAG 13150 0.0 22.427153 6 GGACCGA 550 0.0 21.586668 6 TACATGG 6230 0.0 21.350306 2 GAGTACT 8375 0.0 21.2658 12-13 GTACATG 6670 0.0 21.154833 1 AGAGTAC 11205 0.0 21.150225 10-11 TAAGGTG 1490 0.0 20.399946 5 GTACTTT 8770 0.0 20.172186 14-15 AGTACTT 8815 0.0 20.150442 12-13 ACGCAGA 14585 0.0 20.090328 7 CGCAGAG 14640 0.0 19.85266 8 ACATGGG 6450 0.0 19.736639 3 GTAAGGT 1385 0.0 19.546112 4 GCAGAGT 15755 0.0 18.32709 9 >>END_MODULE