FastQCFastQC Report
Thu 26 May 2016
SRR938087_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938087_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1488481
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60880.40900757214905664No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51950.34901352452601003No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29470.19798707541446614No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA16710.11226209807179266No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA15430.10366272730387557No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA15310.10285653629438334No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26450.061.502531
GTATCAA48750.044.1332781
TATCAAC61750.034.477642
ATCAACG62300.034.325823
TCAACGC62600.034.0854034
CAACGCA64300.033.110335
AACGCAG66050.032.444556
GTGGTAT11800.031.1295641
TGGTATC10700.029.7568872
ACGCAGA76650.027.7042147
CGCAGAG78200.027.155098
GTATAGG3550.026.8760811
GTACATG46500.026.0582371
TAAGGTG8950.025.4867635
GTGTAAG5900.025.0653631
TACATGG47600.024.759462
GCAGAGT83950.024.5029099
CAGAGTA80200.022.39033510-11
GAGTACT56650.022.38964512-13
ACATGGG50900.022.2205923
Produced by FastQC (version 0.11.5)