FastQCFastQC Report
Thu 26 May 2016
SRR938087_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938087_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1488481
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59350.3987286367780308No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52900.3553958700178236No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31200.2096096624679791No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA16590.11145590706230042No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA15840.106417213252974No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA15690.10540947449110873No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29100.057.0423361
GTATCAA51350.043.7186161
ATCAACG67300.033.282223
TATCAAC67550.033.2339172
TCAACGC68200.032.84084
CAACGCA69700.032.134045
GTGGTAT13400.031.2350881
AACGCAG72900.030.6853086
TGGTATC13000.030.3668382
ACGCAGA81750.027.2472177
CGCAGAG83600.026.7010698
GCAGAGT89900.024.4601129
GTACATG50000.023.4958861
GAGTACT58150.022.62388412-13
TACATGG50000.022.5446362
GTACTTT59000.022.05645414-15
CAGAGTA86350.021.69810310-11
ACATGGG50650.021.5950813
AGAGTAC82600.020.75698310-11
CATGGGG33300.020.1350214
Produced by FastQC (version 0.11.5)