##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938087_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1488481 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.482637668871824 33.0 31.0 34.0 31.0 34.0 2 32.70924318147158 34.0 31.0 34.0 31.0 34.0 3 32.772509020941484 34.0 31.0 34.0 31.0 34.0 4 36.23606482044447 37.0 35.0 37.0 35.0 37.0 5 36.15870138752191 37.0 35.0 37.0 35.0 37.0 6 36.20804162095452 37.0 37.0 37.0 35.0 37.0 7 36.179970050003995 37.0 37.0 37.0 35.0 37.0 8 36.169829510756266 37.0 37.0 37.0 35.0 37.0 9 37.860275005189855 39.0 38.0 39.0 35.0 39.0 10-11 37.82996390279755 39.0 38.0 39.0 35.0 39.0 12-13 37.74908614889945 39.0 38.0 39.0 35.0 39.0 14-15 39.00311760781629 40.0 38.0 41.0 35.5 41.0 16-17 38.91146779837969 40.0 38.0 41.0 35.0 41.0 18-19 39.148370385648185 40.0 38.5 41.0 36.0 41.0 20-21 39.11472803482208 40.0 39.0 41.0 36.0 41.0 22-23 39.01495517913901 40.0 38.5 41.0 35.0 41.0 24-25 38.907427773683374 40.0 38.0 41.0 35.0 41.0 26-27 38.74373136103182 40.0 38.0 41.0 35.0 41.0 28-29 38.61683084970517 40.0 38.0 41.0 34.5 41.0 30-31 38.36940343880775 40.0 38.0 41.0 34.0 41.0 32-33 38.14994917637511 40.0 38.0 41.0 34.0 41.0 34-35 38.06478349404527 40.0 38.0 41.0 33.0 41.0 36-37 38.01250872533811 40.0 38.0 41.0 33.0 41.0 38-39 37.828764693670934 40.0 37.0 41.0 33.0 41.0 40-41 37.594215848237226 40.0 37.0 41.0 33.0 41.0 42-43 37.33704595490302 40.0 37.0 41.0 32.0 41.0 44-45 37.06134508938979 40.0 36.0 41.0 31.0 41.0 46-47 36.69107533115975 39.5 36.0 41.0 30.5 41.0 48-49 36.269508646734494 39.0 35.0 41.0 30.0 41.0 50-51 35.872887863533364 39.0 35.0 40.0 28.5 41.0 52-53 35.3268583878464 38.0 35.0 40.0 27.5 41.0 54-55 34.94264522019428 38.0 34.0 40.0 26.0 41.0 56-57 34.54744669229906 38.0 34.0 40.0 26.0 41.0 58-59 34.02861574988192 37.0 33.0 40.0 24.0 41.0 60-61 33.54003880466059 37.0 33.0 39.5 22.5 41.0 62-63 32.97828188603012 36.0 32.0 39.0 21.5 40.5 64-65 32.39776355895708 35.5 31.0 39.0 19.0 40.0 66-67 31.835591787869646 35.0 31.0 38.0 16.5 40.0 68-69 31.21329597085888 35.0 30.0 38.0 10.0 40.0 70-71 30.596949843498173 34.5 29.5 37.0 7.5 39.0 72-73 29.93024264333908 34.0 29.0 36.5 4.5 39.0 74-75 29.278173856434847 34.0 28.0 36.0 2.0 38.5 76-77 28.00687848887557 32.5 26.5 34.5 2.0 36.5 78-79 28.662912055981906 33.5 28.0 35.0 2.0 37.0 80-81 28.396664787793732 34.0 27.5 35.0 2.0 37.0 82-83 27.856261853527187 33.0 26.5 35.0 2.0 36.0 84-85 27.23219308812138 33.0 26.0 35.0 2.0 36.0 86-87 26.535253053280492 32.5 24.5 35.0 2.0 35.0 88-89 25.754632407131833 32.0 22.0 34.5 2.0 35.0 90-91 25.128392636520047 32.0 19.5 34.0 2.0 35.0 92-93 24.212441744301742 31.0 12.0 34.0 2.0 35.0 94-95 23.331332747949084 31.0 2.0 34.0 2.0 35.0 96-97 22.317274120395222 30.0 2.0 34.0 2.0 35.0 98-99 20.942989866850837 29.5 2.0 34.0 2.0 35.0 100-101 17.0963720732747 22.0 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.21975706394771777 1101 2 0.22800707135149167 1101 3 0.2253689400401555 1101 4 0.0948221724925915 1101 5 0.06567593386058945 1101 6 0.13913465990400908 1101 7 0.10507205990913349 1101 8 0.13911179371604732 1101 9 0.11159297532339707 1101 10-11 0.20225107901034534 1101 12-13 0.37381578866724396 1101 14-15 0.49714305887577126 1101 16-17 0.4898901951392034 1101 18-19 0.44489136983372646 1101 20-21 0.3567690668873027 1101 22-23 0.44835807864763666 1101 24-25 0.5439530928019991 1101 26-27 0.5399796650603932 1101 28-29 0.49663875173232697 1101 30-31 0.5486711245335698 1101 32-33 0.4072701083252568 1101 34-35 0.41018955218972764 1101 36-37 0.41743310202527084 1101 38-39 0.509218520590899 1101 40-41 0.7426235711529685 1101 42-43 0.7721042195136292 1101 44-45 0.7531233085025448 1101 46-47 0.7091500157842177 1101 48-49 0.8164741092260073 1101 50-51 0.6815307914669262 1101 52-53 0.7365952934246138 1101 54-55 1.0318982114475688 1101 56-57 1.0813502743506191 1101 58-59 1.2832211491730305 1101 60-61 1.3660189979812287 1101 62-63 1.3069537663078492 1101 64-65 1.4413553055672779 1101 66-67 1.6391271621998094 1101 68-69 1.8520256703898674 1101 70-71 2.0651315843507625 1101 72-73 2.0484395044333183 1101 74-75 1.8699378030318634 1101 76-77 1.962337845509115 1101 78-79 2.361540696239235 1101 80-81 2.1649759322788356 1101 82-83 2.1191097186092698 1101 84-85 2.4197735545174766 1101 86-87 2.649330257022548 1101 88-89 2.807972733175891 1101 90-91 2.9794640429586607 1101 92-93 3.171268289950209 1101 94-95 3.390454191365052 1101 96-97 3.4347754892351077 1101 98-99 3.9238643807876983 1101 100-101 3.952195589567406 1102 1 0.15626131523945475 1102 2 0.15958366212112907 1102 3 0.21009319242166669 1102 4 0.09403623432999808 1102 5 -0.10178752919498635 1102 6 0.0033420765069038794 1102 7 0.07580400702207157 1102 8 0.10384052363524177 1102 9 0.18427004535540448 1102 10-11 0.15467812949754745 1102 12-13 0.272586124150898 1102 14-15 0.356564366743207 1102 16-17 0.2299700208705957 1102 18-19 0.28922527095358674 1102 20-21 0.2701734288144877 1102 22-23 0.32003753433067317 1102 24-25 0.15152830477431678 1102 26-27 0.18137272416626615 1102 28-29 -0.030628623601423044 1102 30-31 0.17641480089999106 1102 32-33 0.15402182056882907 1102 34-35 0.22023090426395697 1102 36-37 0.2576007017097126 1102 38-39 0.2832544877745775 1102 40-41 0.3335874736705762 1102 42-43 0.327751543533374 1102 44-45 0.3128954752754254 1102 46-47 0.32929727702366307 1102 48-49 0.45062463267148445 1102 50-51 0.6768201967598984 1102 52-53 0.7706580429170415 1102 54-55 0.937665884099296 1102 56-57 0.9068314319717885 1102 58-59 0.93153988115332 1102 60-61 0.8841080133256725 1102 62-63 1.0378279877730208 1102 64-65 1.0809037494452554 1102 66-67 1.068780958512125 1102 68-69 1.2612142765963519 1102 70-71 1.2448212128098994 1102 72-73 1.3781399713765339 1102 74-75 1.5689957433171493 1102 76-77 1.5930002131078531 1102 78-79 1.7451401153438688 1102 80-81 1.6987369217909745 1102 82-83 1.8796722063197286 1102 84-85 2.083057390132616 1102 86-87 2.075869321762056 1102 88-89 2.105438482381661 1102 90-91 2.1677468717200874 1102 92-93 2.256908305396532 1102 94-95 2.4121625496723063 1102 96-97 2.5832713090154016 1102 98-99 2.7933885772592078 1102 100-101 2.5766557382974646 1103 1 0.21444920338897333 1103 2 0.2127275883030748 1103 3 0.23268343789296608 1103 4 0.13824394500090875 1103 5 0.08639279414403944 1103 6 0.1384464210636409 1103 7 0.20193136762797792 1103 8 0.18335596807918364 1103 9 0.31140642484616166 1103 10-11 0.3043543601381984 1103 12-13 0.31827055830761 1103 14-15 0.4271753044626223 1103 16-17 0.45469302197134454 1103 18-19 0.3938472795693144 1103 20-21 0.4021980574819466 1103 22-23 0.5088101325033421 1103 24-25 0.5471610989072815 1103 26-27 0.4152748527317982 1103 28-29 0.46352110528845714 1103 30-31 0.5651894374946522 1103 32-33 0.60758826633095 1103 34-35 0.7748838289465354 1103 36-37 0.777986620290605 1103 38-39 0.6989906525515508 1103 40-41 0.9082809176524691 1103 42-43 0.9444313844458634 1103 44-45 0.8883793740066253 1103 46-47 0.9602387108949841 1103 48-49 0.9377197227923872 1103 50-51 1.213623339553621 1103 52-53 1.3223733750212787 1103 54-55 1.4445438596291709 1103 56-57 1.465143324125684 1103 58-59 1.7138890718997786 1103 60-61 1.64687532972048 1103 62-63 1.8077779105394782 1103 64-65 1.824435723226614 1103 66-67 1.9586195381396827 1103 68-69 2.0785654378174137 1103 70-71 1.9284026296490815 1103 72-73 2.1290578275001764 1103 74-75 2.2107600654965367 1103 76-77 2.2270185317267988 1103 78-79 2.2019912516508846 1103 80-81 2.4554365353967604 1103 82-83 2.571128726778692 1103 84-85 2.9688412086858804 1103 86-87 2.924342656937519 1103 88-89 2.8765820144686884 1103 90-91 3.1054413711515956 1103 92-93 3.42180871715777 1103 94-95 3.256778171232469 1103 96-97 3.4267776892762285 1103 98-99 3.7172942843877976 1103 100-101 3.6853862996788784 1104 1 0.24755574686783888 1104 2 0.24922315050599764 1104 3 0.2473588511501248 1104 4 0.16145391235086493 1104 5 0.051450621280565656 1104 6 0.1273952265681757 1104 7 0.16245304131144422 1104 8 0.194320566197149 1104 9 0.20568400793057862 1104 10-11 0.2942739003435051 1104 12-13 0.3220305516364803 1104 14-15 0.41092565458994557 1104 16-17 0.4112718877512336 1104 18-19 0.37760992424406226 1104 20-21 0.47428473083399325 1104 22-23 0.39607661628415514 1104 24-25 0.429015843640407 1104 26-27 0.5322980969100684 1104 28-29 0.6113705216716383 1104 30-31 0.5416993682816909 1104 32-33 0.6156850276264407 1104 34-35 0.673625936381633 1104 36-37 0.7813264608249781 1104 38-39 0.7845125935077917 1104 40-41 0.7308011074375216 1104 42-43 0.8903433939736018 1104 44-45 0.9268826892580577 1104 46-47 0.9678698830250667 1104 48-49 1.0700972530637003 1104 50-51 1.2303850840536796 1104 52-53 1.480274920285531 1104 54-55 1.432063824905093 1104 56-57 1.2563749598136553 1104 58-59 1.3997879743708808 1104 60-61 1.4066339931000442 1104 62-63 1.6603662869536109 1104 64-65 1.6307313867793738 1104 66-67 1.8844123226301654 1104 68-69 1.80293291007985 1104 70-71 2.0178476003239147 1104 72-73 2.105683220123879 1104 74-75 2.0530295748051124 1104 76-77 2.227546662720389 1104 78-79 2.2087575290971238 1104 80-81 2.2342266236395396 1104 82-83 2.5667625588073797 1104 84-85 2.6972364815927605 1104 86-87 3.0123497001095565 1104 88-89 3.21528343229728 1104 90-91 3.260041028614811 1104 92-93 3.5907598532808613 1104 94-95 3.767558885684654 1104 96-97 3.7751340686496135 1104 98-99 3.9911248591097817 1104 100-101 3.915787187452114 1105 1 0.22767820440022035 1105 2 0.24182045531737373 1105 3 0.23902273560251075 1105 4 0.16645939155845468 1105 5 0.011792788520217812 1105 6 0.016502682200631114 1105 7 0.13254849883279718 1105 8 0.08060821005768304 1105 9 0.17859777332424187 1105 10-11 0.17782043651178725 1105 12-13 0.2529784996657227 1105 14-15 0.2537821815643966 1105 16-17 0.3949971874533631 1105 18-19 0.392093480104414 1105 20-21 0.2969664445175866 1105 22-23 0.4265734445598852 1105 24-25 0.34551607875005175 1105 26-27 0.4038668246210193 1105 28-29 0.4348508060490275 1105 30-31 0.36677629274682744 1105 32-33 0.4247687316710227 1105 34-35 0.43066970086292855 1105 36-37 0.369697105907683 1105 38-39 0.4264185556676665 1105 40-41 0.6265224629122699 1105 42-43 0.6456895064432331 1105 44-45 0.6966085885148274 1105 46-47 0.7456331231066002 1105 48-49 0.9060857211614746 1105 50-51 0.8675106506478585 1105 52-53 0.9146194255877802 1105 54-55 0.9448549924024121 1105 56-57 0.9613730197831529 1105 58-59 0.9561716773126037 1105 60-61 1.1768667065259564 1105 62-63 1.282758149052036 1105 64-65 1.2791053705192184 1105 66-67 1.1540840815009155 1105 68-69 1.5824609763557014 1105 70-71 1.5825656642637078 1105 72-73 1.5845892735203364 1105 74-75 1.445856675477156 1105 76-77 1.3779689577313547 1105 78-79 1.5528011341650654 1105 80-81 1.6874874289075983 1105 82-83 1.8322735287524452 1105 84-85 2.055723538531403 1105 86-87 2.284596135115528 1105 88-89 2.381619241049375 1105 90-91 2.48703387105526 1105 92-93 2.402128983435354 1105 94-95 2.4389600789788695 1105 96-97 2.7284720740977377 1105 98-99 2.7226168678885436 1105 100-101 2.7903010519026346 1106 1 0.12761353173505796 1106 2 0.16025910960508583 1106 3 0.1339144631439524 1106 4 0.06617769652696381 1106 5 0.011063593369364355 1106 6 0.10126727803987734 1106 7 0.12448394770015625 1106 8 0.13400439306830236 1106 9 0.13452921161827192 1106 10-11 0.14044050413595954 1106 12-13 0.04930300886963579 1106 14-15 -0.011844513922838473 1106 16-17 -0.018174334003639103 1106 18-19 -0.01776193959513961 1106 20-21 -0.10477884935240667 1106 22-23 0.024389765509916117 1106 24-25 -0.01374708691180615 1106 26-27 -0.19426417876019286 1106 28-29 -0.016499290091879004 1106 30-31 0.040135929190959985 1106 32-33 0.023262162403995035 1106 34-35 0.009283882840527724 1106 36-37 -0.019855871422159055 1106 38-39 -0.10650807775117244 1106 40-41 -0.12024715916498252 1106 42-43 0.06130035929772504 1106 44-45 0.1568414101094291 1106 46-47 0.3221096347758916 1106 48-49 0.39385217037173703 1106 50-51 0.48272999515350534 1106 52-53 0.38398454835732565 1106 54-55 0.452175156818555 1106 56-57 0.6000112478389426 1106 58-59 0.8026413744795988 1106 60-61 0.7939871669606191 1106 62-63 0.2903040167291593 1106 64-65 0.6993952255916724 1106 66-67 0.7703451333649234 1106 68-69 0.6445619264796214 1106 70-71 0.4334582772564026 1106 72-73 0.9183089066657288 1106 74-75 1.3362343377990342 1106 76-77 1.3390025918576889 1106 78-79 1.3964256681095328 1106 80-81 1.1291474763920952 1106 82-83 1.7561203804089764 1106 84-85 1.9202929010499012 1106 86-87 1.9605274866006113 1106 88-89 1.7387751195342105 1106 90-91 2.09972490499322 1106 92-93 2.1592657639519537 1106 94-95 2.4844023922636715 1106 96-97 3.040246405560339 1106 98-99 3.465675407501635 1106 100-101 3.3813853390492703 1107 1 0.20835887334996528 1107 2 0.14714661823172293 1107 3 0.14081577439309 1107 4 0.06341717202730734 1107 5 -0.0703718793704553 1107 6 -0.0208859310698557 1107 7 0.02165441008801139 1107 8 0.0442873468295204 1107 9 0.028939149506470585 1107 10-11 0.07522311283161542 1107 12-13 0.12487236704769344 1107 14-15 0.3207986882855778 1107 16-17 0.1795482332841445 1107 18-19 0.1834112833172057 1107 20-21 0.06602860406373878 1107 22-23 0.19519721892606867 1107 24-25 0.19191198831248357 1107 26-27 0.18047702206796856 1107 28-29 0.10944964020487191 1107 30-31 0.15642302373892392 1107 32-33 0.056043390837402285 1107 34-35 0.10555717476599114 1107 36-37 0.23514757923346252 1107 38-39 0.3082607283219829 1107 40-41 0.3379999077777356 1107 42-43 0.6140953903536328 1107 44-45 0.47016094082025717 1107 46-47 0.4677273021326869 1107 48-49 0.2861917148851916 1107 50-51 0.4226885872860109 1107 52-53 0.4861239548445653 1107 54-55 0.4728790905659679 1107 56-57 0.5013224969762788 1107 58-59 0.455505418647995 1107 60-61 0.5676241579889023 1107 62-63 0.6153557765635256 1107 64-65 0.7014656189664237 1107 66-67 0.9083713583476616 1107 68-69 1.0776192073629893 1107 70-71 1.0911532392991923 1107 72-73 1.0169976575283926 1107 74-75 1.0543157732717745 1107 76-77 1.1543925159432646 1107 78-79 1.018923942781722 1107 80-81 1.376904550236123 1107 82-83 1.52251099462568 1107 84-85 1.6580430735826859 1107 86-87 1.6962072657586518 1107 88-89 1.886808245828206 1107 90-91 2.1556255261112334 1107 92-93 2.2638206293763865 1107 94-95 2.319806118971748 1107 96-97 2.554049028058614 1107 98-99 2.7096367601586415 1107 100-101 2.7768304736248375 1108 1 0.14029793609410035 1108 2 0.1360721932960942 1108 3 0.14134280142673816 1108 4 0.146539033314383 1108 5 -4.599800698414924E-4 1108 6 0.0783224997953198 1108 7 0.12399561503050904 1108 8 0.07539712567898249 1108 9 0.20063143037269526 1108 10-11 0.14247612156331257 1108 12-13 0.1803409827347764 1108 14-15 0.30912670481738047 1108 16-17 0.3452803575900347 1108 18-19 0.14587358692494234 1108 20-21 0.253548594110967 1108 22-23 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2316 62-63 0.5845823436957929 2316 64-65 0.7972274362469136 2316 66-67 1.0317059553013728 2316 68-69 1.406454440986952 2316 70-71 1.4417684280160294 2316 72-73 1.1975956415678937 2316 74-75 1.708144251386841 2316 76-77 1.8199088992673644 2316 78-79 1.8868139883692585 2316 80-81 1.819978841393695 2316 82-83 2.217375447040652 2316 84-85 2.250865515480445 2316 86-87 2.318337535298596 2316 88-89 2.5782543135388174 2316 90-91 2.962355373796374 2316 92-93 2.841545918308043 2316 94-95 3.3564559697673424 2316 96-97 3.7258362732690067 2316 98-99 3.995167334911507 2316 100-101 4.2364202830125635 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 5.0 4 9.0 5 36.0 6 52.0 7 143.0 8 351.0 9 700.0 10 1243.0 11 2055.0 12 3129.0 13 4747.0 14 6480.0 15 8998.0 16 11745.0 17 16096.0 18 16749.0 19 13897.0 20 13755.0 21 14465.0 22 15665.0 23 17212.0 24 19091.0 25 21435.0 26 24178.0 27 28110.0 28 33320.0 29 40255.0 30 47725.0 31 56903.0 32 70033.0 33 87113.0 34 114469.0 35 156541.0 36 218901.0 37 270450.0 38 145312.0 39 7110.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.41802145946102 18.39546490684127 14.898141125079864 23.288372508617847 2 16.0572607305077 19.220442389872876 39.69656247480312 25.025734404816298 3 19.6105832248858 22.879999140034034 29.56811568692133 27.94130194815883 4 13.474951930118284 16.32756853065387 35.56344888468469 34.63403065454316 5 14.22263236944998 36.642387415594904 33.84483655057512 15.29014366437999 6 33.65592170810376 35.104646952161296 16.49547424522046 14.743957094514476 7 29.604140059564077 32.21975960727749 19.61637400813312 18.55972632502531 8 27.089446952595935 34.74826668816511 18.707204665161775 19.45508169407718 9 26.406584968165532 16.669006860013667 19.66434237319791 37.2600657986229 10-11 25.731131267379293 26.006579862289136 26.320490486610176 21.94179838372139 12-13 26.391804799658175 23.20802213800512 27.127991556492827 23.27218150584388 14-15 23.06710666780429 24.780867206232394 25.29252976692346 26.859496359039852 16-17 22.69726654219973 28.00126437623322 26.28122226618949 23.020246815377558 18-19 22.684535442508167 26.124619662595627 28.68135367532404 22.509491219572165 20-21 22.97738432670622 25.40764712482054 29.18371144811388 22.43125710035936 22-23 22.939818788233225 25.233602478233664 29.11854009410274 22.708038639430374 24-25 22.894510983575444 25.456331910733994 29.012427169464 22.63672993622657 26-27 22.434443227241868 25.86823104632503 28.7741314330192 22.923194293413903 28-29 22.587893295245287 26.142994099353633 28.755422474321136 22.51369013107994 30-31 22.635055469300582 25.691359177577677 28.737484724359934 22.936100628761803 32-33 22.372371565374365 25.742115619883627 28.813098722791892 23.072414091950115 34-35 23.006652191601663 25.931251392102368 28.519696715696668 22.542399700599304 36-37 23.169550270646493 25.506441836701104 28.436827540707828 22.88718035194458 38-39 22.659004160919338 25.84046472221681 28.481334987936492 23.01919612892736 40-41 23.066283818763377 25.616744254173916 28.57508082101995 22.741891106042754 42-43 22.74486947349326 25.703391908733902 28.319095401422338 23.2326432163505 44-45 22.744504175721257 25.955749884693592 28.48825822992148 22.81148770966367 46-47 23.242218073324416 26.20046880007202 27.74049517595455 22.816817950649018 48-49 23.04705266644317 26.200670347824396 27.70381348502265 23.048463500709783 50-51 23.10936451321851 26.42596714368541 27.62144763688619 22.843220706209888 52-53 23.38414128228711 26.194859054297638 27.516340484023644 22.904659179391608 54-55 22.984572863207525 26.46335425175061 27.51439218908404 23.037680695957828 56-57 23.05881736441962 26.53534930420654 27.399116078443754 23.006717252930088 58-59 23.033481784450053 26.1988900093451 27.37243538882928 23.395192817375566 60-61 22.767136429689057 26.43292054114228 27.6974647308229 23.102478298345762 62-63 22.7940094633388 26.503865349977595 27.77072733881051 22.9313978478731 64-65 22.526689961108 26.459390479287276 27.62524345288922 23.388676106715504 66-67 22.589446082766955 26.516571765636165 27.43670474688785 23.457277404709032 68-69 22.868615857896415 26.22775096601294 27.706154702483467 23.19747847360718 70-71 22.71557379637362 26.376386396601635 27.344386659957365 23.563653147067377 72-73 22.809905806626286 26.470921184389045 27.13861552099608 23.58055748798859 74-75 22.754842016794303 26.87672869186775 26.92278235328499 23.445646938052956 76-77 22.804758676798695 26.39593652858182 27.34663727652553 23.45266751809395 78-79 22.86267006431389 26.54608960409975 27.30518562212081 23.286054709465557 80-81 23.016649882195182 26.660100679955846 27.07646530451555 23.246784133333424 82-83 22.849445479266148 26.71478167688145 27.122085259977258 23.313687583875144 84-85 22.939642068311038 26.716854366649027 26.95821890772887 23.38528465731106 86-87 22.989531279183357 26.65223987089787 26.93612884414089 23.422100005777885 88-89 22.74550363760102 26.870816624464805 26.62220747191264 23.761472266021535 90-91 22.861055888978544 26.773828583483432 26.79804868610142 23.5670668414366 92-93 22.922067168498344 26.40293238641689 26.862360642279153 23.812639802805613 94-95 22.682333655809952 26.282684349134687 26.99754111576911 24.03744087928625 96-97 22.91723492050269 25.999578092521354 26.966135878393903 24.117051108582054 98-99 22.671703568940416 26.271514382783522 26.604236130659377 24.452545917616682 100-101 23.07970340232761 25.61221809347919 26.78146378757942 24.526614716613782 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 612.0 1 411.0 2 295.5 3 375.5 4 300.5 5 272.5 6 508.5 7 1128.5 8 1846.0 9 2184.0 10 1845.0 11 1375.5 12 1348.0 13 1463.0 14 1595.5 15 1685.5 16 1791.0 17 1931.0 18 2063.0 19 2251.0 20 2523.5 21 2944.0 22 3487.5 23 4280.5 24 5305.5 25 6477.0 26 7973.0 27 9782.0 28 11344.5 29 12956.0 30 14841.0 31 17470.5 32 21338.5 33 23221.0 34 23516.5 35 24980.0 36 27366.0 37 30018.5 38 32383.0 39 34418.5 40 36737.5 41 39890.0 42 42125.0 43 43697.0 44 45860.0 45 49178.5 46 51384.5 47 52770.5 48 54172.5 49 54728.5 50 55244.5 51 55211.0 52 56306.5 53 58483.0 54 59747.0 55 57022.5 56 52318.5 57 48443.5 58 45159.5 59 40125.5 60 32578.5 61 27329.0 62 23327.5 63 17656.5 64 12706.5 65 9497.5 66 6860.5 67 5209.0 68 3909.5 69 2706.0 70 1981.0 71 1401.0 72 972.0 73 691.5 74 508.0 75 361.0 76 238.5 77 145.0 78 85.5 79 46.5 80 24.5 81 13.5 82 9.5 83 5.5 84 3.0 85 1.0 86 1.0 87 0.5 88 1.5 89 1.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.013503699408994808 3 0.003359129206217614 4 0.0015451994348601025 5 0.1177038873858652 6 0.0 7 0.0 8 6.718258412435228E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 1.0077387618652842E-4 24-25 5.710519650569943E-4 26-27 0.006415936783875642 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.011723360929699472 36-37 5.374606729948182E-4 38-39 0.007255719085430045 40-41 0.010849987336082893 42-43 0.005777702234694296 44-45 0.0037286334189015513 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 3.359129206217614E-5 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 3.359129206217614E-5 68-69 0.0011756952221761649 70-71 0.0 72-73 3.359129206217614E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.3436516824870455E-4 82-83 0.0022506165681658013 84-85 0.004602007012518131 86-87 0.003157581453844557 88-89 0.0 90-91 0.0032247640379689095 92-93 3.359129206217614E-5 94-95 6.718258412435228E-5 96-97 2.0154775237305684E-4 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1488481.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.05144279152782 #Duplication Level Percentage of deduplicated Percentage of total 1 80.12381498515263 47.314268768347 2 11.65233561809239 13.761744602789298 3 3.4594618357698677 6.128586380533146 4 1.489372744205415 3.517984375988275 5 0.804114428357945 2.3742058582010643 6 0.48846354955490673 1.7306686411372914 7 0.3384088258013186 1.3988470591868267 8 0.25086420280089583 1.1851114496111466 9 0.19122611554259394 1.0162960219988617 >10 1.0954369635645211 11.943803601537528 >50 0.06931997684887908 2.8062523354687694 >100 0.03250474048068672 3.649415788369025 >500 0.003307424414940488 1.3628470358134435 >1k 0.0011404911775656854 1.0539907150860046 >5k 2.2809823551313708E-4 0.755977365932294 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5935 0.3987286367780308 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5290 0.3553958700178236 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3120 0.2096096624679791 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 1659 0.11145590706230042 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 1584 0.106417213252974 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 1569 0.10540947449110873 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 6.718258412435228E-5 0.0 10-11 0.0 0.0 0.0 1.679564603108807E-4 0.0 12-13 0.0 0.0 0.0 2.0154775237305684E-4 3.359129206217614E-5 14-15 0.0 0.0 0.0 2.3513904443523299E-4 1.3436516824870455E-4 16-17 0.0 0.0 0.0 2.687303364974091E-4 1.3436516824870455E-4 18-19 0.0 0.0 0.0 3.695042126839375E-4 1.3436516824870455E-4 20-21 0.0 0.0 0.0 5.374606729948182E-4 2.0154775237305684E-4 22-23 0.0 0.0 0.0 6.718258412435228E-4 2.0154775237305684E-4 24-25 0.0 0.0 0.0 9.069648856787557E-4 2.0154775237305684E-4 26-27 0.0 0.0 0.0 0.001444425558673574 2.0154775237305684E-4 28-29 0.0 0.0 0.0 0.003963772463336785 2.0154775237305684E-4 30-31 0.0 0.0 0.0 0.012999830028062164 2.0154775237305684E-4 32-33 0.0 0.0 0.0 0.034061570151046605 2.0154775237305684E-4 34-35 0.0 0.0 0.0 0.06436091559112948 2.0154775237305684E-4 36-37 0.0 0.0 0.0 0.11461348851614497 2.0154775237305684E-4 38-39 0.0 0.0 0.0 0.18717067937044546 2.0154775237305684E-4 40-41 0.0 0.0 0.0 0.2624823561738443 2.0154775237305684E-4 42-43 0.0 0.0 0.0 0.3347372253995852 2.0154775237305684E-4 44-45 0.0 0.0 0.0 0.4244259752055955 2.3513904443523299E-4 46-47 0.0 0.0 0.0 0.5283910241380306 2.687303364974091E-4 48-49 0.0 0.0 0.0 0.6339684550894502 3.359129206217614E-4 50-51 0.0 0.0 0.0 0.748716308773844 3.359129206217614E-4 52-53 0.0 0.0 0.0 0.8569810430902376 3.359129206217614E-4 54-55 0.0 0.0 0.0 0.9754575301935329 3.359129206217614E-4 56-57 0.0 0.0 0.0 1.105926108563025 3.359129206217614E-4 58-59 0.0 0.0 0.0 1.2462369355067346 3.359129206217614E-4 60-61 0.0 0.0 0.0 1.4008240615768692 3.359129206217614E-4 62-63 0.0 0.0 0.0 1.5513802325995427 3.359129206217614E-4 64-65 0.0 0.0 0.0 1.7061017238379261 3.359129206217614E-4 66-67 0.0 0.0 0.0 1.8779883653200813 3.695042126839375E-4 68-69 0.0 0.0 0.0 2.0632107497509207 4.030955047461137E-4 70-71 0.0 0.0 0.0 2.261768877130444 4.030955047461137E-4 72-73 0.0 0.0 0.0 2.4684225058969513 4.702780888704659E-4 74-75 0.0 0.0 0.0 2.667618867825656 4.702780888704659E-4 76-77 0.0 0.0 0.0 2.8809235724204743 4.702780888704659E-4 78-79 0.0 0.0 0.0 3.1228144665602047 4.702780888704659E-4 80-81 0.0 0.0 0.0 3.370785384563189 5.038693809326421E-4 82-83 0.0 0.0 3.359129206217614E-5 3.628867281476888 5.374606729948182E-4 84-85 0.0 0.0 6.718258412435228E-5 3.885538344123976 5.374606729948182E-4 86-87 0.0 0.0 6.718258412435228E-5 4.148860482599375 5.374606729948182E-4 88-89 0.0 0.0 6.718258412435228E-5 4.437208133661095 5.374606729948182E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2910 0.0 57.042336 1 GTATCAA 5135 0.0 43.718616 1 ATCAACG 6730 0.0 33.28222 3 TATCAAC 6755 0.0 33.233917 2 TCAACGC 6820 0.0 32.8408 4 CAACGCA 6970 0.0 32.13404 5 GTGGTAT 1340 0.0 31.235088 1 AACGCAG 7290 0.0 30.685308 6 TGGTATC 1300 0.0 30.366838 2 ACGCAGA 8175 0.0 27.247217 7 CGCAGAG 8360 0.0 26.701069 8 GCAGAGT 8990 0.0 24.460112 9 GTACATG 5000 0.0 23.495886 1 GAGTACT 5815 0.0 22.623884 12-13 TACATGG 5000 0.0 22.544636 2 GTACTTT 5900 0.0 22.056454 14-15 CAGAGTA 8635 0.0 21.698103 10-11 ACATGGG 5065 0.0 21.595081 3 AGAGTAC 8260 0.0 20.756983 10-11 CATGGGG 3330 0.0 20.135021 4 >>END_MODULE