FastQCFastQC Report
Thu 26 May 2016
SRR938086_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938086_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1459537
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59020.4043748120123025No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51480.35271459373760305No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30350.20794265578741752No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA16470.11284400463982758No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA14860.10181310922573392No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA14680.10057984141546257No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28100.056.9214441
GTATCAA48700.041.961621
GTGGTAT12500.033.6132161
ATCAACG61750.032.7909663
TATCAAC62800.032.3172872
TCAACGC62700.032.2183274
CAACGCA64450.031.122265
TGGTATC13250.030.8495792
AACGCAG66150.030.3911696
ACGCAGA75900.026.541597
CGCAGAG77350.026.0440438
TACGCTC1309.972055E-625.5938874
GTACATG47500.023.4206921
GCAGAGT84050.023.401079
TACATGG48850.022.4757442
GAGTACT55150.022.39603412-13
ACGTCGT652.9414168E-421.92483122-23
CAGAGTA78600.021.66681110-11
TAGACCG1100.002866682421.605234
AACGTCG550.002877078321.5926422-23
Produced by FastQC (version 0.11.5)