FastQCFastQC Report
Thu 26 May 2016
SRR938086_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938086_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1459537
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58180.3986195622310363No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52660.36079934938271524No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32200.22061790828187297No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA16250.11133667731616258No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA14890.10201865386077913No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA14860.10181310922573392No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28300.054.3876841
GTATCAA48700.041.8475761
GTGGTAT12600.035.8174861
TGGTATC12950.031.9147532
ATCAACG63000.031.8931333
TATCAAC64100.031.4231592
TCAACGC64650.031.223964
CAACGCA65600.030.844195
AACGCAG68000.029.7555716
TAAGGTG9600.025.7276295
ACGCAGA78050.025.680727
GTAAGGT8900.025.6164464
CGCAGAG79000.025.251658
GTACATG51650.023.9136051
TACATGG52550.022.7808442
GCAGAGT86600.022.7613419
GAGTACT54500.022.17986512-13
AGGTAAG8900.021.8844622
GTGTAAG6850.021.4987451
GGTAAGG9550.021.3876193
Produced by FastQC (version 0.11.5)