##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938086_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1459537 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.49566814681642 33.0 31.0 34.0 31.0 34.0 2 32.72757045556228 34.0 31.0 34.0 31.0 34.0 3 32.68842447981792 34.0 31.0 34.0 31.0 34.0 4 36.2177293210107 37.0 35.0 37.0 35.0 37.0 5 36.17672179602162 37.0 35.0 37.0 35.0 37.0 6 36.23440036121044 37.0 37.0 37.0 35.0 37.0 7 36.192463774470944 37.0 37.0 37.0 35.0 37.0 8 36.18299433313441 37.0 37.0 37.0 35.0 37.0 9 37.88991097861856 39.0 38.0 39.0 35.0 39.0 10-11 37.853505255433745 39.0 38.0 39.0 35.0 39.0 12-13 37.79773037613983 39.0 38.0 39.0 35.0 39.0 14-15 39.086168421903665 40.0 38.5 41.0 36.0 41.0 16-17 39.013991080733135 40.0 38.0 41.0 36.0 41.0 18-19 39.00156727784222 40.0 38.5 41.0 35.5 41.0 20-21 38.99787158530411 40.0 38.5 41.0 35.5 41.0 22-23 39.0281637258939 40.0 39.0 41.0 35.0 41.0 24-25 38.9817469512592 40.0 38.5 41.0 35.0 41.0 26-27 38.8133421763203 40.0 38.0 41.0 35.0 41.0 28-29 38.732049615734304 40.0 38.0 41.0 35.0 41.0 30-31 38.516865965028636 40.0 38.0 41.0 34.0 41.0 32-33 38.31342062585601 40.0 38.0 41.0 34.0 41.0 34-35 38.112116719206156 40.0 38.0 41.0 34.0 41.0 36-37 38.037966834687985 40.0 38.0 41.0 33.0 41.0 38-39 37.996738006641834 40.0 38.0 41.0 33.0 41.0 40-41 37.86878784162375 40.0 37.5 41.0 33.0 41.0 42-43 37.68215982191613 40.0 37.0 41.0 33.0 41.0 44-45 37.435563812359675 40.0 37.0 41.0 32.5 41.0 46-47 37.07646979829905 40.0 36.5 41.0 31.5 41.0 48-49 36.69866128779195 40.0 36.0 41.0 31.0 41.0 50-51 36.38597993747332 39.0 35.5 41.0 30.0 41.0 52-53 35.91571128378383 39.0 35.0 40.0 29.0 41.0 54-55 35.58705534700388 38.5 35.0 40.0 28.0 41.0 56-57 35.30457329961487 38.0 35.0 40.0 28.0 41.0 58-59 34.91843954623967 38.0 34.0 40.0 26.5 41.0 60-61 34.49375315596659 37.5 34.0 40.0 26.0 41.0 62-63 34.04877197357793 37.0 33.5 40.0 25.5 41.0 64-65 33.59405414182717 36.0 33.0 39.0 24.5 41.0 66-67 33.07837211389639 35.5 32.5 39.0 23.0 40.0 68-69 32.546236237930245 35.0 32.0 38.5 21.5 40.0 70-71 31.952726104237165 35.0 31.0 37.5 19.5 40.0 72-73 31.482937739844896 35.0 31.0 37.0 18.5 39.0 74-75 30.93250085472311 34.5 30.0 36.5 17.0 39.0 76-77 29.752718841660062 33.5 29.0 35.0 16.0 37.0 78-79 30.435036932945174 34.0 30.0 35.0 15.0 37.5 80-81 30.253944572833714 34.0 30.0 35.0 10.0 37.0 82-83 29.807886336557416 34.0 30.0 35.0 7.0 37.0 84-85 29.312176738239593 34.0 29.0 35.0 2.0 36.0 86-87 28.781177524105246 34.0 29.0 35.0 2.0 36.0 88-89 28.1703920489854 33.5 27.5 35.0 2.0 35.0 90-91 27.662054816013573 33.0 27.0 35.0 2.0 35.0 92-93 27.043375741759203 33.0 25.5 35.0 2.0 35.0 94-95 26.31394990329125 32.0 24.5 34.5 2.0 35.0 96-97 25.504660724599653 32.0 21.5 34.0 2.0 35.0 98-99 24.52169660652659 32.0 10.0 34.0 2.0 35.0 100-101 21.070319217669713 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.049840639532398257 1101 2 0.054449172247750255 1101 3 0.143231982100815 1101 4 0.04534839745387842 1101 5 -0.03651078790700524 1101 6 0.06460139523426989 1101 7 0.013489916845927041 1101 8 0.13140005488057227 1101 9 0.11120350945369495 1101 10-11 0.1686516679921155 1101 12-13 0.14164037777151606 1101 14-15 0.29295647290143734 1101 16-17 0.18034732524568398 1101 18-19 0.20719918909041724 1101 20-21 0.1895347159389118 1101 22-23 0.1734387854946604 1101 24-25 0.18152960351638825 1101 26-27 0.18785718328936696 1101 28-29 0.21541396195929252 1101 30-31 0.2723212606491643 1101 32-33 0.20701282019253142 1101 34-35 0.07970786193310886 1101 36-37 0.1420830682688532 1101 38-39 0.1961122018574244 1101 40-41 0.09484551058179136 1101 42-43 0.06538037117578455 1101 44-45 0.1501233379273046 1101 46-47 0.07447951666621577 1101 48-49 0.13399761959364298 1101 50-51 0.30373682357235054 1101 52-53 0.10038260251418052 1101 54-55 0.13008697977747374 1101 56-57 0.2724717058939419 1101 58-59 0.3493073885014013 1101 60-61 0.38435171660879774 1101 62-63 0.4132002293872361 1101 64-65 0.3633630936833967 1101 66-67 0.31151171825712964 1101 68-69 0.44274006983866343 1101 70-71 0.466671068101963 1101 72-73 0.2693788190183568 1101 74-75 0.4033364248308473 1101 76-77 0.6537517271292685 1101 78-79 0.772056570804029 1101 80-81 0.9197816794022202 1101 82-83 0.8765284318725186 1101 84-85 0.9971714012479893 1101 86-87 1.1455061064415197 1101 88-89 1.5569688678746765 1101 90-91 1.4459419414963897 1101 92-93 1.5993004071641792 1101 94-95 1.6281208251929584 1101 96-97 1.7617636221214568 1101 98-99 2.1688943492503405 1101 100-101 2.594919526796975 1102 1 -0.0818204624895813 1102 2 -0.015190317948736265 1102 3 0.1343186088958106 1102 4 0.05791833058595586 1102 5 -0.05960898901834355 1102 6 -0.010358896683179353 1102 7 0.010825177785918072 1102 8 -0.0014974340068647507 1102 9 -0.11921833164564788 1102 10-11 0.01032907622157353 1102 12-13 0.03737853055530849 1102 14-15 0.11343554027530445 1102 16-17 0.14743150746524947 1102 18-19 0.009850154366922936 1102 20-21 0.057135848859715566 1102 22-23 0.03252484615462237 1102 24-25 -0.017925898215366942 1102 26-27 -0.02157482166919067 1102 28-29 -0.10885777635183302 1102 30-31 -0.10251219315958338 1102 32-33 -0.041014790278360636 1102 34-35 -0.038933489893246076 1102 36-37 0.0869581091428202 1102 38-39 0.1652396760546253 1102 40-41 0.021419585609869785 1102 42-43 0.0365652364990936 1102 44-45 0.11092201130588109 1102 46-47 0.13317074694164432 1102 48-49 0.23896849129518216 1102 50-51 0.3873192870514828 1102 52-53 0.3132035547164449 1102 54-55 0.40435934170478305 1102 56-57 0.4923829029496005 1102 58-59 0.4300849348482956 1102 60-61 0.4546967065035048 1102 62-63 0.49207872889078175 1102 64-65 0.4991351820329939 1102 66-67 0.3537462597609604 1102 68-69 0.4510550426462885 1102 70-71 0.6671154081886108 1102 72-73 0.6784715767738341 1102 74-75 0.6070295340889444 1102 76-77 0.5797509907199014 1102 78-79 0.8482944624824391 1102 80-81 0.843023203780465 1102 82-83 0.9065194897587396 1102 84-85 1.0032256298266375 1102 86-87 0.9938318054474742 1102 88-89 0.9995321906752217 1102 90-91 1.1010398953303522 1102 92-93 1.4337642029911564 1102 94-95 1.3160171942826366 1102 96-97 1.6783476370491996 1102 98-99 1.9171150096002272 1102 100-101 2.4030428596404505 1103 1 0.22158823034040864 1103 2 0.21906184830408648 1103 3 0.3295737167190538 1103 4 0.19675684815810257 1103 5 0.03520900453184339 1103 6 0.051165516805802724 1103 7 0.14914595835816158 1103 8 0.16670398371674366 1103 9 0.15771944718534314 1103 10-11 0.2659691253679526 1103 12-13 0.2723390434497972 1103 14-15 0.40317700663012346 1103 16-17 0.29158713251032964 1103 18-19 0.4036641631452724 1103 20-21 0.3588036188299455 1103 22-23 0.3393255534709496 1103 24-25 0.45867934406494015 1103 26-27 0.45059810248877596 1103 28-29 0.4508661487643266 1103 30-31 0.4553040256602827 1103 32-33 0.3941190099627292 1103 34-35 0.28356256912362454 1103 36-37 0.3940289541102189 1103 38-39 0.45783384752087386 1103 40-41 0.48745114438461457 1103 42-43 0.32362722069394323 1103 44-45 0.4764530265855669 1103 46-47 0.5165430822703598 1103 48-49 0.47068220076487677 1103 50-51 0.5399071349140812 1103 52-53 0.5869930547009901 1103 54-55 0.7292576617641302 1103 56-57 0.6617878660125456 1103 58-59 0.7327395604806526 1103 60-61 0.7566413756154731 1103 62-63 0.8741435207141492 1103 64-65 0.8918795873824266 1103 66-67 0.9386892571748504 1103 68-69 1.0554856220995958 1103 70-71 1.1841701414896626 1103 72-73 1.0439803757270276 1103 74-75 0.9887469140821459 1103 76-77 0.8101175536674461 1103 78-79 1.2542424492324962 1103 80-81 1.3160474318114126 1103 82-83 1.3153720197153653 1103 84-85 1.5647909342353898 1103 86-87 1.5903495089619035 1103 88-89 1.7533643460066273 1103 90-91 1.8222855293392328 1103 92-93 2.1641401032353436 1103 94-95 2.1470237162233516 1103 96-97 2.349513807443916 1103 98-99 2.7152481298285487 1103 100-101 2.8388270508888915 1104 1 0.14779079261196415 1104 2 0.18966607741457153 1104 3 0.1922392026298425 1104 4 0.11730247012204131 1104 5 0.021220805826601463 1104 6 0.05220024418273539 1104 7 0.049276561859080914 1104 8 0.03383018991993936 1104 9 0.1339594757489877 1104 10-11 0.15093439564203237 1104 12-13 0.1520587392094086 1104 14-15 0.2539020204891216 1104 16-17 0.16157968623159036 1104 18-19 0.17753424319864308 1104 20-21 0.15379282524924776 1104 22-23 0.28701808793054084 1104 24-25 0.1873353024973241 1104 26-27 0.1575394671544217 1104 28-29 0.16038023796934198 1104 30-31 0.18727939186685916 1104 32-33 0.1959290781772225 1104 34-35 0.36744008371081094 1104 36-37 0.4099515409991241 1104 38-39 0.2258945969406767 1104 40-41 0.22830537584408006 1104 42-43 0.3097083170116761 1104 44-45 0.25325757468805676 1104 46-47 0.16237651347802284 1104 48-49 0.22925657898885987 1104 50-51 0.23656552924269647 1104 52-53 0.5174731165262401 1104 54-55 0.34562132909164944 1104 56-57 0.5648811253685437 1104 58-59 0.581255942293879 1104 60-61 0.6675494105495616 1104 62-63 0.6761917171643645 1104 64-65 0.6895830446168247 1104 66-67 0.6500408389805656 1104 68-69 0.7311910806682249 1104 70-71 0.6116978242640876 1104 72-73 0.7358553581096565 1104 74-75 0.5063410965887947 1104 76-77 0.6078377474116365 1104 78-79 0.964169078722577 1104 80-81 1.1256476112737808 1104 82-83 1.1557021928824511 1104 84-85 1.2956822444046097 1104 86-87 1.2811589335548241 1104 88-89 1.4146743507985526 1104 90-91 1.2802698428252164 1104 92-93 1.4626093291981945 1104 94-95 1.738051537587829 1104 96-97 2.0178807469059024 1104 98-99 2.170882578616002 1104 100-101 2.586383814083135 1105 1 0.10121469083439649 1105 2 0.0633199736205654 1105 3 0.12454427241005561 1105 4 0.0646934960079193 1105 5 0.03147220289646668 1105 6 0.06027309168248962 1105 7 0.02283549906444904 1105 8 -0.0032220304240269115 1105 9 -0.047842151434331015 1105 10-11 0.06969737033710288 1105 12-13 0.07025520218650172 1105 14-15 0.08210861894046673 1105 16-17 0.07134909308871329 1105 18-19 0.05300670642090921 1105 20-21 0.10687250819810856 1105 22-23 0.07966413958148166 1105 24-25 0.1583450480089823 1105 26-27 0.2114955443972164 1105 28-29 0.1472545323280272 1105 30-31 0.11927377960338248 1105 32-33 0.17891240749840875 1105 34-35 0.25460587288761616 1105 36-37 0.17768595604191262 1105 38-39 0.13221518883105432 1105 40-41 0.1624736495889394 1105 42-43 0.24409711060598482 1105 44-45 0.31706753587834413 1105 46-47 0.270360175910767 1105 48-49 0.15044838106907577 1105 50-51 0.026896899734694557 1105 52-53 0.22662964253692763 1105 54-55 0.42163671973379024 1105 56-57 0.46222413877561763 1105 58-59 0.36152090366626055 1105 60-61 0.33466703623699345 1105 62-63 0.3168478492151152 1105 64-65 0.3477863830164196 1105 66-67 0.4994240052103365 1105 68-69 0.5381900209293278 1105 70-71 0.4600552289786357 1105 72-73 0.2596939313617703 1105 74-75 0.36892369715072704 1105 76-77 0.43797172932056583 1105 78-79 0.6085054272336556 1105 80-81 0.7437529793442792 1105 82-83 0.5476210398012604 1105 84-85 0.9090203419734273 1105 86-87 0.9904598079060314 1105 88-89 1.0433966503520082 1105 90-91 1.0903565051273851 1105 92-93 1.2403137730839973 1105 94-95 1.2879156724882392 1105 96-97 1.5438860997865191 1105 98-99 1.7743870010050067 1105 100-101 2.2042664415604847 1106 1 -0.009298419344936804 1106 2 0.00840294809431441 1106 3 0.02238350095638708 1106 4 0.03040233811914561 1106 5 -0.004712706585927151 1106 6 0.012002346293364496 1106 7 -0.006036465975071792 1106 8 0.0031869774236668036 1106 9 0.032822458872487914 1106 10-11 0.019597946165070823 1106 12-13 -0.027649718911611387 1106 14-15 -0.06506261728357288 1106 16-17 -0.05343178418130634 1106 18-19 -0.03981987940792209 1106 20-21 -0.07179928048081763 1106 22-23 -0.12792609471605232 1106 24-25 -0.06027480636957705 1106 26-27 -0.11036608690289285 1106 28-29 -0.12155106729800735 1106 30-31 -0.0458150558500563 1106 32-33 -0.05845815515564112 1106 34-35 -0.22428973084641513 1106 36-37 0.021805587313686203 1106 38-39 -0.12296009164603561 1106 40-41 -0.07863085291835858 1106 42-43 -0.035757144004882946 1106 44-45 0.08364605175787432 1106 46-47 -0.18891841008804278 1106 48-49 0.008988501334258103 1106 50-51 -0.14322423308112775 1106 52-53 -0.23368700972813627 1106 54-55 -0.1814452525301462 1106 56-57 0.05658015699246022 1106 58-59 0.016382241032047773 1106 60-61 -0.03512049882264279 1106 62-63 0.04007193695628075 1106 64-65 0.043662626039903785 1106 66-67 -0.009341346660924899 1106 68-69 -0.29073665839186447 1106 70-71 -0.18414346354480315 1106 72-73 0.012013976266434412 1106 74-75 0.13278066445673176 1106 76-77 0.26182848414392623 1106 78-79 0.39767217653246334 1106 80-81 0.5103930266570806 1106 82-83 0.333075982855906 1106 84-85 0.5440574910229188 1106 86-87 0.5586078622732593 1106 88-89 0.8390326109152291 1106 90-91 0.8001614283503216 1106 92-93 0.6189835254708846 1106 94-95 1.1049574890732181 1106 96-97 1.1379605784957576 1106 98-99 1.4881073596757162 1106 100-101 1.7411485597291652 1107 1 0.142376976268892 1107 2 0.10171934034151064 1107 3 0.15678699639711624 1107 4 0.09356595391371769 1107 5 0.060650893534017314 1107 6 0.02588716508110167 1107 7 -0.04090014787565366 1107 8 0.06619171729308704 1107 9 0.07542974484574927 1107 10-11 -0.01650458460488835 1107 12-13 -0.03710901259950816 1107 14-15 0.01247799191402521 1107 16-17 0.1539705135138405 1107 18-19 0.09030333675193702 1107 20-21 0.11388088737394497 1107 22-23 0.06533773643419494 1107 24-25 0.07732281926592321 1107 26-27 0.16866603925737422 1107 28-29 0.17280427979265056 1107 30-31 0.059593157311169875 1107 32-33 0.07823519812308177 1107 34-35 0.1291157085882091 1107 36-37 0.23976142065838246 1107 38-39 0.19924475692347698 1107 40-41 0.12527021125217885 1107 42-43 0.16461372266541474 1107 44-45 0.08504036902585455 1107 46-47 0.3598718328004509 1107 48-49 0.17588595693418085 1107 50-51 0.09356286977165951 1107 52-53 0.2043175171708711 1107 54-55 0.215332462587007 1107 56-57 0.5020305553323183 1107 58-59 0.4445688320743386 1107 60-61 0.41859826267775446 1107 62-63 0.5197666992472278 1107 64-65 0.44508756845270625 1107 66-67 0.6538755543299786 1107 68-69 0.6022073763921725 1107 70-71 0.5120683040073679 1107 72-73 0.5421639841037482 1107 74-75 0.7232196135073927 1107 76-77 0.6279951104881931 1107 78-79 0.7204531100584397 1107 80-81 0.8970429339723616 1107 82-83 0.8215255327216511 1107 84-85 0.9555150434647537 1107 86-87 0.961691631202072 1107 88-89 1.070479480761346 1107 90-91 1.0680149507105092 1107 92-93 1.2171360497665482 1107 94-95 1.2709466258785937 1107 96-97 1.3139991499924832 1107 98-99 1.5580800653880935 1107 100-101 1.8992605600153993 1108 1 0.1205936292525891 1108 2 2.242150868880799E-5 1108 3 0.12082359391501285 1108 4 0.009829003346688125 1108 5 0.00938666402079491 1108 6 -0.019289394016134054 1108 7 -0.013866357770702109 1108 8 -0.046679200614356375 1108 9 -0.026926475273945982 1108 10-11 0.05234954325893426 1108 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-4.092572071845151 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 3.0 4 12.0 5 22.0 6 50.0 7 159.0 8 297.0 9 594.0 10 1047.0 11 1696.0 12 2706.0 13 3901.0 14 5526.0 15 7386.0 16 9442.0 17 13306.0 18 13765.0 19 10403.0 20 9510.0 21 9765.0 22 10201.0 23 10874.0 24 12298.0 25 14339.0 26 16911.0 27 20332.0 28 24612.0 29 29525.0 30 36069.0 31 44361.0 32 55705.0 33 73029.0 34 100692.0 35 148746.0 36 229058.0 37 317434.0 38 210011.0 39 15746.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.53510736624012 18.341432933868752 14.90609693347959 23.21736276641154 2 16.13770590206329 19.2325323718232 39.76925675513629 24.860504970977225 3 19.82730326831511 22.981533289891924 29.445943881099357 27.745219560693613 4 13.440289626093755 16.37861869894357 35.56497711260489 34.616114562357794 5 14.24189397697344 36.59536070466424 33.87510517069855 15.287640147663772 6 33.66389546252434 35.13052796303477 16.495470472082186 14.710106102358697 7 29.58671140231457 32.19959480300945 19.601421546695974 18.61227224798001 8 27.076207781103033 34.72021245108034 18.77521349250721 19.428366275309415 9 26.379392917068905 16.69323902031946 19.662331273547707 37.26503678906393 10-11 25.709420179139002 26.032810404943486 26.319613685709918 21.938155730207594 12-13 26.40621649194231 23.23373782233681 27.110480926485593 23.24956475923529 14-15 23.07108350113769 24.803276655542135 25.280551298117143 26.845088545203033 16-17 22.67486972747855 28.0193061290348 26.34675460325932 22.959069540227325 18-19 22.68986671800715 26.182515414134755 28.640041328174622 22.487576539683474 20-21 23.050357750437296 25.3393028063009 29.196176595728645 22.414162847533156 22-23 22.922988591587607 25.184938785381938 29.149723508208425 22.74234911482203 24-25 22.838787387963293 25.55010738189277 29.020962850215877 22.590142379928064 26-27 22.428717200866487 25.885659696975722 28.806426433793874 22.879196668363917 28-29 22.512280456542822 26.15895684548738 28.789692878106 22.539069819863798 30-31 22.585278756208304 25.770227133673213 28.724109083908118 22.920385026210365 32-33 22.370813296320318 25.732440827647256 28.80646720610297 23.09027866992946 34-35 22.98127154495278 25.937099445043476 28.54228061537576 22.539348394627986 36-37 23.180185840823174 25.505011919110387 28.418290541248894 22.896511698817545 38-39 22.627268275985184 25.769095423923773 28.50420135818159 23.099434941909454 40-41 23.0545951706249 25.6072697617522 28.61718276172821 22.720952305894688 42-43 22.750790299446912 25.72411850688398 28.349529932013347 23.175561261655762 44-45 22.75835180267514 25.959666315038266 28.464153602797857 22.817828279488737 46-47 23.270768828104284 26.160237669295178 27.8028128676327 22.766180634967835 48-49 23.07966894901693 26.19326654715359 27.687408339719504 23.03965616410997 50-51 23.049535571897113 26.508886037147395 27.627494198502678 22.814084192452814 52-53 23.364327180468873 26.179466502048253 27.608960923909432 22.84724539357344 54-55 22.890032935102024 26.45780819533866 27.62221855286985 23.02994031668947 56-57 23.008426644888072 26.537559513736202 27.50139256490243 22.95262127647329 58-59 22.978554157928166 26.185598583660436 27.46346957973659 23.372377678674813 60-61 22.670785324387115 26.435951949145515 27.816286945791713 23.076975780675653 62-63 22.74375366982817 26.463803247194146 27.905356287644643 22.88708679533304 64-65 22.46846088862427 26.458493344122143 27.705327100306466 23.36771866694712 66-67 22.546279341995234 26.542282174407095 27.534654052133785 23.37678443146389 68-69 22.81017584367624 26.22920998811265 27.805670984251833 23.154943183959276 70-71 22.61278747986519 26.385422226363563 27.4748773069816 23.526912986789647 72-73 22.73183208099555 26.495662665626153 27.26268672873658 23.50981852464172 74-75 22.68996949032467 26.86060716514895 27.020555148653308 23.428868195873076 76-77 22.821072710044348 26.38864242564594 27.392488165767638 23.397796698542074 78-79 22.782704378169242 26.580895174291573 27.313490511031922 23.322909936507262 80-81 22.91408664885962 26.694881141898325 27.1464634785954 23.244568730646655 82-83 22.782243836530604 26.72694646964393 27.18846585229324 23.30234384153222 84-85 22.851407933927106 26.66835219965481 27.08209573416267 23.398144132255414 86-87 22.997455678202044 26.638744330773484 26.966145480942593 23.397654510081882 88-89 22.707063952472602 26.92586758677581 26.734539788987878 23.632528671763716 90-91 22.78712410152475 26.83577408903748 26.80929217228114 23.567809637156632 92-93 22.871809066779214 26.420872255787152 26.957039477200134 23.7502792002335 94-95 22.617229933770027 26.30192962075948 27.10797373547954 23.972866709990953 96-97 22.90704529906302 26.025884297962325 27.08934053243725 23.977729870537402 98-99 22.638793396113623 26.336993061828963 26.749316305986596 24.274897236070817 100-101 22.902258173700822 25.847003558757876 26.934432249992806 24.3163060175485 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 552.0 1 377.0 2 283.5 3 362.5 4 277.5 5 243.5 6 503.5 7 1157.5 8 1940.0 9 2266.5 10 1881.0 11 1403.5 12 1313.0 13 1413.5 14 1544.5 15 1670.5 16 1808.5 17 1911.5 18 2064.5 19 2255.5 20 2462.5 21 2830.5 22 3395.0 23 4149.0 24 5150.5 25 6349.5 26 7711.5 27 9379.0 28 11094.5 29 12767.5 30 14682.5 31 17114.0 32 20784.5 33 22809.5 34 23097.0 35 24525.0 36 26913.0 37 29540.5 38 31905.0 39 33995.5 40 36358.0 41 39659.0 42 41620.5 43 42784.0 44 44750.5 45 47988.5 46 50521.0 47 52231.5 48 53539.0 49 53669.0 50 54289.0 51 54705.0 52 55576.0 53 57743.5 54 59084.0 55 56133.5 56 51505.0 57 47694.5 58 44186.5 59 38788.0 60 31627.5 61 26486.5 62 22311.0 63 16975.5 64 12054.0 65 8825.5 66 6338.5 67 4891.0 68 3558.0 69 2345.5 70 1782.0 71 1234.0 72 832.5 73 610.5 74 465.0 75 320.0 76 199.5 77 113.0 78 57.0 79 35.0 80 19.0 81 10.0 82 4.0 83 1.5 84 2.0 85 2.5 86 1.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.011373469805835687 3 0.0068514878348407745 4 0.0 5 3.4257439174203877E-4 6 2.0554463504522327E-4 7 0.0 8 6.851487834840775E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.4257439174203874E-5 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0018156442762328052 26-27 0.02151367180140003 28-29 1.7128719587101938E-4 30-31 0.0 32-33 0.0034600013565945914 34-35 0.015998224094353208 36-37 0.007947725888415299 38-39 0.020999810213786975 40-41 0.02343208839515545 42-43 0.01918416593755417 44-45 0.009352280894557656 46-47 0.002055446350452232 48-49 2.398020742194271E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.398020742194271E-4 68-69 1.370297566968155E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.7128719587101938E-4 82-83 6.851487834840775E-4 84-85 0.0039738629442076494 86-87 8.564359793550968E-4 88-89 0.0 90-91 0.0034600013565945914 92-93 7.536636618324852E-4 94-95 1.0277231752261164E-4 96-97 2.398020742194271E-4 98-99 1.7128719587101938E-4 100-101 0.0026720802555879023 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1459537.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.250085428403864 #Duplication Level Percentage of deduplicated Percentage of total 1 80.26165070589761 47.55509660949145 2 11.624747702555153 13.775345889200688 3 3.360367777957073 5.973062337444368 4 1.4883356320065255 3.5273605337009637 5 0.7669867883746947 2.272201636682889 6 0.5182894413262082 1.8425216205130532 7 0.339620250577749 1.408577020196329 8 0.24561814602849255 1.1642316907963488 9 0.18664249606794614 0.9952725452936708 >10 1.102509289283625 11.927347381762777 >50 0.0674290593568305 2.7207595392195243 >100 0.03328038249956167 3.7353815992289943 >500 0.003130842508949053 1.2844343396215037 >1k 0.0011595712996107603 1.0568843779832893 >5k 2.3191425992215208E-4 0.7615228788641213 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5818 0.3986195622310363 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5266 0.36079934938271524 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3220 0.22061790828187297 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 1625 0.11133667731616258 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 1489 0.10201865386077913 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 1486 0.10181310922573392 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 6.851487834840775E-5 0.0 7 0.0 0.0 0.0 6.851487834840775E-5 0.0 8 0.0 0.0 0.0 6.851487834840775E-5 0.0 9 0.0 0.0 0.0 1.370297566968155E-4 0.0 10-11 0.0 0.0 0.0 2.0554463504522324E-4 0.0 12-13 0.0 0.0 0.0 3.4257439174203877E-4 6.851487834840775E-5 14-15 0.0 0.0 0.0 3.4257439174203877E-4 6.851487834840775E-5 16-17 0.0 0.0 0.0 3.4257439174203877E-4 1.370297566968155E-4 18-19 0.0 0.0 0.0 3.4257439174203877E-4 1.370297566968155E-4 20-21 0.0 0.0 0.0 4.1108927009044654E-4 2.0554463504522324E-4 22-23 0.0 0.0 0.0 6.166339051356698E-4 2.0554463504522324E-4 24-25 0.0 0.0 0.0 9.592082968777085E-4 2.0554463504522324E-4 26-27 0.0 0.0 0.0 0.0015415847628391743 2.0554463504522324E-4 28-29 0.0 0.0 0.0 0.004624754288517523 2.0554463504522324E-4 30-31 0.0 0.0 0.0 0.012949312007849064 2.0554463504522324E-4 32-33 0.0 0.0 0.0 0.03384634990411343 2.0554463504522324E-4 34-35 0.0 0.0 0.0 0.06341051991145137 2.74059513393631E-4 36-37 0.0 0.0 0.0 0.11239865793056292 2.74059513393631E-4 38-39 0.0 0.0 0.0 0.18420225043969424 2.74059513393631E-4 40-41 0.0 0.0 0.0 0.2622406968785307 2.74059513393631E-4 42-43 0.0 0.0 0.0 0.3348664679278429 2.74059513393631E-4 44-45 0.0 0.0 0.0 0.4283207619950711 3.4257439174203877E-4 46-47 0.0 0.0 0.0 0.533011496111438 4.110892700904465E-4 48-49 0.0 0.0 0.0 0.6399632212133026 4.7960414843885426E-4 50-51 0.0 0.0 0.0 0.7524646514613881 4.7960414843885426E-4 52-53 0.0 0.0 0.0 0.8645892498785575 4.7960414843885426E-4 54-55 0.0 0.0 0.0 0.9807562261182827 4.7960414843885426E-4 56-57 0.0 0.0 0.0 1.114908357924465 4.7960414843885426E-4 58-59 0.0 0.0 0.0 1.2610163360024447 4.7960414843885426E-4 60-61 0.0 0.0 0.0 1.4183264966903888 4.7960414843885426E-4 62-63 0.0 0.0 0.0 1.5702239819888089 6.166339051356698E-4 64-65 0.0 0.0 0.0 1.7244852305902487 6.166339051356698E-4 66-67 0.0 0.0 0.0 1.9009110423373987 6.166339051356698E-4 68-69 0.0 0.0 0.0 2.0940887418407343 6.166339051356698E-4 70-71 0.0 0.0 0.0 2.293467037834601 6.166339051356698E-4 72-73 0.0 0.0 0.0 2.493975829321216 6.166339051356698E-4 74-75 0.0 0.0 0.0 2.692223629822334 6.166339051356698E-4 76-77 0.0 0.0 0.0 2.9075658924713794 6.166339051356698E-4 78-79 0.0 0.0 0.0 3.1584331195440747 7.194062226582814E-4 80-81 0.0 0.0 0.0 3.4083068808807173 7.536636618324852E-4 82-83 0.0 0.0 0.0 3.666984804085131 8.906934185293008E-4 84-85 0.0 0.0 0.0 3.9324799576852114 8.906934185293008E-4 86-87 0.0 0.0 0.0 4.203593331309861 8.906934185293008E-4 88-89 0.0 0.0 0.0 4.496974040397743 8.906934185293008E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2830 0.0 54.387684 1 GTATCAA 4870 0.0 41.847576 1 GTGGTAT 1260 0.0 35.817486 1 TGGTATC 1295 0.0 31.914753 2 ATCAACG 6300 0.0 31.893133 3 TATCAAC 6410 0.0 31.423159 2 TCAACGC 6465 0.0 31.22396 4 CAACGCA 6560 0.0 30.84419 5 AACGCAG 6800 0.0 29.755571 6 TAAGGTG 960 0.0 25.727629 5 ACGCAGA 7805 0.0 25.68072 7 GTAAGGT 890 0.0 25.616446 4 CGCAGAG 7900 0.0 25.25165 8 GTACATG 5165 0.0 23.913605 1 TACATGG 5255 0.0 22.780844 2 GCAGAGT 8660 0.0 22.761341 9 GAGTACT 5450 0.0 22.179865 12-13 AGGTAAG 890 0.0 21.884462 2 GTGTAAG 685 0.0 21.498745 1 GGTAAGG 955 0.0 21.387619 3 >>END_MODULE