FastQCFastQC Report
Thu 26 May 2016
SRR938085_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938085_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1488648
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61920.41594789365921286No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53320.3581773528732111No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31520.21173574948543913No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA16390.11009990273053133No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA15820.10627092502727307No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA15200.1021060720868869No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28950.058.365741
GTATCAA49800.043.705771
TATCAAC64550.033.273532
ATCAACG65200.032.9418143
TCAACGC65250.032.916574
CAACGCA65500.032.7909365
GTGGTAT11450.032.5156481
AACGCAG67700.031.7253516
TGGTATC12500.028.8908462
ACGCAGA77300.027.3697557
CGCAGAG77650.027.1851588
TCGCACG458.9418265E-426.39347622-23
GCAGAGT84350.024.6304389
TAAGGTG9150.023.888755
TATTACG1000.001650263223.7589192
GTGTAGC6900.023.5197731
GAGTACT55650.022.97797612-13
GTACATG50550.022.8505951
GTAAGGT8550.022.7863314
TACATGG50200.022.6230352
Produced by FastQC (version 0.11.5)