FastQCFastQC Report
Thu 26 May 2016
SRR938085_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938085_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1488648
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60280.40493118588141724No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53790.36133458010221353No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31690.21287772529167406No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA16300.10949532730370108No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA15690.10539764941074048No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA14950.10042669590124731No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29050.056.08641
GTATCAA50900.043.208861
ATCAACG65100.033.56163
TCAACGC65700.033.25514
TATCAAC67000.032.613142
CAACGCA67250.032.5592545
AACGCAG69700.031.4147766
TGGTATC12500.028.8810632
TAAGGTG7900.028.8589135
GTGGTAT13400.028.0047631
ACGCAGA78700.027.5808287
CGCAGAG79800.027.141128
GTAAGGT7700.025.9074344
GCAGAGT87500.024.2098799
GTACATG49200.024.040521
TACATGG48600.023.8486162
GGACCGA4300.023.196196
GAGTACT57900.022.76408612-13
ACATGGG49950.022.0606483
GTACTTT59850.021.9033614-15
Produced by FastQC (version 0.11.5)