##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938085_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1488648 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.50907199015482 34.0 31.0 34.0 31.0 34.0 2 32.728084812527875 34.0 31.0 34.0 31.0 34.0 3 32.78688447504044 34.0 31.0 34.0 31.0 34.0 4 36.24685889478238 37.0 35.0 37.0 35.0 37.0 5 36.20590562711937 37.0 37.0 37.0 35.0 37.0 6 36.23878109532945 37.0 37.0 37.0 35.0 37.0 7 36.202469623443555 37.0 37.0 37.0 35.0 37.0 8 36.19336203051359 37.0 37.0 37.0 35.0 37.0 9 37.903364663775456 39.0 38.0 39.0 35.0 39.0 10-11 37.88749422294592 39.0 38.0 39.0 35.0 39.0 12-13 37.840841488384086 39.0 38.0 39.0 35.0 39.0 14-15 39.17300832701888 40.5 39.0 41.0 36.0 41.0 16-17 39.120051886006635 40.0 38.5 41.0 36.0 41.0 18-19 39.14178771610213 40.0 39.0 41.0 36.0 41.0 20-21 39.059938279566424 40.0 39.0 41.0 35.5 41.0 22-23 38.92391149553151 40.0 38.0 41.0 35.0 41.0 24-25 38.891964050601615 40.0 38.0 41.0 35.0 41.0 26-27 38.85566198322236 40.0 38.0 41.0 35.0 41.0 28-29 38.79537137053219 40.0 38.0 41.0 35.0 41.0 30-31 38.596509047135385 40.0 38.0 41.0 34.5 41.0 32-33 38.42178036715194 40.0 38.0 41.0 34.0 41.0 34-35 38.23426222988913 40.0 38.0 41.0 34.0 41.0 36-37 38.10781763049425 40.0 38.0 41.0 33.5 41.0 38-39 38.1013137424025 40.0 38.0 41.0 33.5 41.0 40-41 38.02920771062064 40.0 38.0 41.0 33.0 41.0 42-43 37.87915007442996 40.0 37.5 41.0 33.0 41.0 44-45 37.67970164874436 40.0 37.0 41.0 33.0 41.0 46-47 37.358515915112235 40.0 37.0 41.0 32.5 41.0 48-49 37.01703827902902 40.0 36.5 41.0 31.5 41.0 50-51 36.71191913736491 40.0 36.0 41.0 31.0 41.0 52-53 36.29106477824173 39.0 35.0 41.0 30.0 41.0 54-55 36.06142956561928 39.0 35.0 41.0 29.5 41.0 56-57 35.81417568155803 39.0 35.0 40.5 29.0 41.0 58-59 35.47084099128874 38.0 35.0 40.0 28.0 41.0 60-61 35.10963807427948 38.0 34.5 40.0 28.0 41.0 62-63 34.73763240201848 37.0 34.0 40.0 27.0 41.0 64-65 34.33880507682139 37.0 34.0 40.0 26.0 41.0 66-67 33.91749157624905 36.0 33.5 39.0 26.0 41.0 68-69 33.46931678946265 36.0 33.0 39.0 25.5 40.0 70-71 32.959799428743395 35.0 32.5 38.5 24.5 40.0 72-73 32.546122387562406 35.0 32.0 37.5 23.5 39.5 74-75 32.1031610562067 35.0 32.0 37.0 22.5 39.0 76-77 30.828976695632548 34.0 30.5 35.5 21.0 38.0 78-79 31.536575133947046 35.0 31.5 36.0 22.0 38.5 80-81 31.379048640108337 35.0 32.0 36.0 21.5 37.0 82-83 31.02392002676254 35.0 31.0 35.0 20.0 37.0 84-85 30.655444739118984 34.0 31.0 35.0 18.5 36.5 86-87 30.238192977789243 34.0 31.0 35.0 15.5 36.0 88-89 29.79062276642967 34.0 30.0 35.0 8.5 36.0 90-91 29.46631406484273 34.0 30.0 35.0 4.5 35.0 92-93 29.07019355818165 34.0 29.0 35.0 2.0 35.0 94-95 28.665170006610026 34.0 29.0 35.0 2.0 35.0 96-97 28.1025883889274 34.0 29.0 35.0 2.0 35.0 98-99 27.422126990396656 33.5 27.5 35.0 2.0 35.0 100-101 25.00964768031126 31.0 22.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.07424386389615023 1101 2 0.06976636063347286 1101 3 0.13634995063232935 1101 4 0.0996987391955031 1101 5 0.10953707010598634 1101 6 0.033897516068421396 1101 7 1.205103715307132E-5 1101 8 0.04948325329011993 1101 9 -0.07946845399958846 1101 10-11 0.13243360013355954 1101 12-13 0.13472988262938657 1101 14-15 0.08707804039570988 1101 16-17 0.12741956920736897 1101 18-19 0.13997307173795548 1101 20-21 0.1533915651069364 1101 22-23 0.23848094367964023 1101 24-25 0.25111129498187523 1101 26-27 0.11608987567139906 1101 28-29 0.05492575733882887 1101 30-31 0.17007230671808315 1101 32-33 0.18038997472228857 1101 34-35 0.03839211832493561 1101 36-37 0.12667321225426065 1101 38-39 0.021094333902333062 1101 40-41 0.12866166288942082 1101 42-43 0.2648630610053644 1101 44-45 0.23048215553769325 1101 46-47 0.4195066775081173 1101 48-49 0.35580117999771943 1101 50-51 0.2834809345774474 1101 52-53 0.04504471731123516 1101 54-55 0.1541775427453871 1101 56-57 0.08690815959341336 1101 58-59 0.02817349499886035 1101 60-61 0.2929836088997959 1101 62-63 0.21898834116988297 1101 64-65 0.3332925739323187 1101 66-67 0.2725234761179749 1101 68-69 0.18529451064426183 1101 70-71 0.43614911112472043 1101 72-73 0.1676098786298965 1101 74-75 0.3232998950629806 1101 76-77 0.19529952873506673 1101 78-79 0.21371660198867204 1101 80-81 0.37152758726687196 1101 82-83 0.42993573710320376 1101 84-85 0.16184228198432749 1101 86-87 0.17152327517511168 1101 88-89 0.09973030036937303 1101 90-91 0.21125766582772343 1101 92-93 0.3299045337290245 1101 94-95 0.14075631773453168 1101 96-97 0.28767203453370627 1101 98-99 0.4412860848285547 1101 100-101 0.3736792168069911 1102 1 0.06403188049323916 1102 2 0.10843308870735058 1102 3 0.04601387468160567 1102 4 0.01659657544818316 1102 5 0.05355297314542895 1102 6 -0.0029527010346299676 1102 7 0.0189526052872111 1102 8 0.05220373552236879 1102 9 0.05114254060443102 1102 10-11 0.12537105688967642 1102 12-13 -0.0033110274254681826 1102 14-15 0.010823137636876368 1102 16-17 0.10926436676708562 1102 18-19 0.0705624845636379 1102 20-21 0.03493866265228718 1102 22-23 0.14904991004633672 1102 24-25 0.029190098997908365 1102 26-27 0.02701523592109112 1102 28-29 0.07692016000019208 1102 30-31 0.09521272215928178 1102 32-33 0.07947373986970518 1102 34-35 0.12276749988863855 1102 36-37 0.1293870003865294 1102 38-39 -0.01557621355723171 1102 40-41 0.28399503977903606 1102 42-43 0.20982256437812197 1102 44-45 0.12155549288392109 1102 46-47 0.013004778525832705 1102 48-49 0.05524251718971129 1102 50-51 -0.05655497793012643 1102 52-53 -0.14940041141898774 1102 54-55 -0.002604437380092861 1102 56-57 -0.04640055730292403 1102 58-59 -0.035723028139159396 1102 60-61 -0.2351696335080149 1102 62-63 -0.19904615325955888 1102 64-65 -0.0742083525311159 1102 66-67 -0.2719936275752346 1102 68-69 -0.08374672286950613 1102 70-71 0.053762841205760026 1102 72-73 -0.027784741241625 1102 74-75 0.07377838932961112 1102 76-77 0.06002649514032399 1102 78-79 0.08976248816969346 1102 80-81 0.054343993274212465 1102 82-83 0.15217771435825966 1102 84-85 -0.05701033856837867 1102 86-87 -0.036347272712873746 1102 88-89 -0.09376774709554425 1102 90-91 -0.0917352394973534 1102 92-93 -0.03277537690206245 1102 94-95 -0.29828854665906945 1102 96-97 0.010423834443312074 1102 98-99 -0.13207000487452447 1102 100-101 0.09021942746228362 1103 1 0.06576535285244489 1103 2 0.06943988109949828 1103 3 0.05579911729724785 1103 4 0.036533596099999954 1103 5 0.07916137234114018 1103 6 0.09312865938612447 1103 7 0.07828748447411016 1103 8 0.040241209853142834 1103 9 0.042508073012797354 1103 10-11 0.0261574372270843 1103 12-13 0.08551898837941252 1103 14-15 0.09869295595122196 1103 16-17 0.11277960853164615 1103 18-19 0.09339576159392493 1103 20-21 0.15722704457592585 1103 22-23 0.10873284124367188 1103 24-25 0.18022436066139136 1103 26-27 0.06312184655970299 1103 28-29 0.14625696584763404 1103 30-31 0.16801412313911612 1103 32-33 0.13172644463482186 1103 34-35 0.18890625808542438 1103 36-37 0.19565550794515474 1103 38-39 0.10442967607175291 1103 40-41 0.18838986212683295 1103 42-43 0.3796214032649132 1103 44-45 0.31422468004723925 1103 46-47 0.2522388448081827 1103 48-49 0.2112310115279925 1103 50-51 0.26199119221853806 1103 52-53 0.33963537590901893 1103 54-55 0.2496445080473748 1103 56-57 0.2613202868145805 1103 58-59 0.2970652882571372 1103 60-61 0.18055149882535915 1103 62-63 0.16714805516751596 1103 64-65 0.18419938000871383 1103 66-67 0.1727326221565164 1103 68-69 0.22623651152885316 1103 70-71 0.17602929365979492 1103 72-73 0.1758538798959819 1103 74-75 -0.010627617047681781 1103 76-77 0.07319696909359763 1103 78-79 0.07908309753744192 1103 80-81 0.24209915179033814 1103 82-83 0.22782341975880627 1103 84-85 0.3471427374835443 1103 86-87 0.4445586858895112 1103 88-89 0.26142308889238564 1103 90-91 0.29411612777897034 1103 92-93 0.32414214375210904 1103 94-95 0.2752331329712199 1103 96-97 0.3470934058944799 1103 98-99 0.3608919407080151 1103 100-101 0.5956984010169144 1104 1 0.18667972065183136 1104 2 0.18710933979033229 1104 3 0.2023323553244083 1104 4 0.08333526451629325 1104 5 0.06641179094771843 1104 6 0.10405996849782895 1104 7 0.12707984329691868 1104 8 0.14031958620643792 1104 9 0.07630842957347994 1104 10-11 0.14212759214230886 1104 12-13 0.13947329954683596 1104 14-15 0.23577718811666415 1104 16-17 0.14529150907693378 1104 18-19 0.24369337990847129 1104 20-21 0.245935184534126 1104 22-23 0.30898343867247746 1104 24-25 0.2951526174796655 1104 26-27 0.4072605416697783 1104 28-29 0.4175169827248979 1104 30-31 0.38385575265787963 1104 32-33 0.32312928283420206 1104 34-35 0.35856593919071855 1104 36-37 0.4548221175496252 1104 38-39 0.2928280052908576 1104 40-41 0.4058734048168233 1104 42-43 0.4462568211990643 1104 44-45 0.41042808220168325 1104 46-47 0.4283839105216529 1104 48-49 0.32569250785130066 1104 50-51 0.4305109432073593 1104 52-53 0.528849242858378 1104 54-55 0.5454657331126285 1104 56-57 0.42233640101251524 1104 58-59 0.38788850598329816 1104 60-61 0.4652598831246664 1104 62-63 0.38657450381229097 1104 64-65 0.5575402866029222 1104 66-67 0.36216915479847245 1104 68-69 0.39154977801977964 1104 70-71 0.611152294286903 1104 72-73 0.45167805084805934 1104 74-75 0.37295394091264455 1104 76-77 0.4780115728102423 1104 78-79 0.36446686826271346 1104 80-81 0.5353432185131979 1104 82-83 0.6255506533001451 1104 84-85 0.6070519765826532 1104 86-87 0.6025355611706118 1104 88-89 0.6600062552675006 1104 90-91 0.4656527033284057 1104 92-93 0.4946508609661855 1104 94-95 0.5463303378763662 1104 96-97 0.5724973446141242 1104 98-99 0.44336322446933707 1104 100-101 0.6295150141436459 1105 1 0.12451153300989404 1105 2 0.07215274887177969 1105 3 0.10031893250561552 1105 4 -0.044948862607327555 1105 5 -0.03568720118452973 1105 6 0.01612015379008369 1105 7 -0.014064195297152082 1105 8 -0.022427235009196522 1105 9 -0.037641218667261 1105 10-11 0.03562201163546064 1105 12-13 0.08543743444107577 1105 14-15 0.026606470398263582 1105 16-17 -0.04381042941491842 1105 18-19 0.03300531012092023 1105 20-21 0.12534928913220966 1105 22-23 0.13716326374105137 1105 24-25 0.16996031779279974 1105 26-27 -0.07758004501194193 1105 28-29 0.02330193085139598 1105 30-31 0.10405496727034347 1105 32-33 0.08073292577427083 1105 34-35 0.15110984549925632 1105 36-37 0.2566211545479504 1105 38-39 0.23841656027955338 1105 40-41 0.14316844318424415 1105 42-43 0.15484219855972725 1105 44-45 0.17079145113493155 1105 46-47 0.2119041858235775 1105 48-49 0.1497520548427289 1105 50-51 0.10144674772079298 1105 52-53 0.16804900788967103 1105 54-55 0.12928480720641033 1105 56-57 0.2762432183428487 1105 58-59 0.2051175403451566 1105 60-61 0.10744462958511036 1105 62-63 0.28496926974553105 1105 64-65 0.30584763307538054 1105 66-67 0.3176331023652992 1105 68-69 0.180194036318845 1105 70-71 0.22368773617146331 1105 72-73 0.09675087727186593 1105 74-75 0.22109014875818644 1105 76-77 0.23203957478989778 1105 78-79 0.3032681578153955 1105 80-81 0.2152501561031066 1105 82-83 0.3076414585305116 1105 84-85 0.37288068069321767 1105 86-87 0.46394981378047717 1105 88-89 0.4411058440722613 1105 90-91 0.3605250200366221 1105 92-93 0.5509637948835469 1105 94-95 0.38966841992115064 1105 96-97 0.6301349840111605 1105 98-99 0.8282008046616554 1105 100-101 1.0533284915417838 1106 1 -0.13489644908093368 1106 2 -0.04038004911978277 1106 3 -0.05357581483089291 1106 4 -0.09229624715159446 1106 5 0.00391373959102026 1106 6 -0.040972142817047086 1106 7 -0.02432753088122297 1106 8 -0.14960549747470253 1106 9 -0.10521391804959279 1106 10-11 0.005528976365447136 1106 12-13 -0.08099282989147838 1106 14-15 -0.2610619379418324 1106 16-17 -0.15427837008623868 1106 18-19 -0.023187222586436462 1106 20-21 0.085740417926921 1106 22-23 0.06408027519258752 1106 24-25 0.01143759824451962 1106 26-27 -0.03831286203653406 1106 28-29 0.019329465303449922 1106 30-31 0.1274005663350124 1106 32-33 -0.04534346012496826 1106 34-35 -0.017224628305399392 1106 36-37 -0.09180408868966339 1106 38-39 -0.14090858280805918 1106 40-41 -0.05490174338477516 1106 42-43 -0.21447542194881208 1106 44-45 -0.08762862599416366 1106 46-47 -0.19536552582599853 1106 48-49 -0.14114449156841147 1106 50-51 -0.09917567772183844 1106 52-53 -0.26769797061659517 1106 54-55 -0.508245158488819 1106 56-57 -0.31909550164047573 1106 58-59 -0.20369656585009466 1106 60-61 -0.2676361475063729 1106 62-63 -0.3104294949201005 1106 64-65 -0.46267581154075543 1106 66-67 -0.5806754502633069 1106 68-69 -0.6594916467630583 1106 70-71 -0.48518188303196297 1106 72-73 -0.40078207932828747 1106 74-75 -0.5940003261159461 1106 76-77 -0.4859412018484157 1106 78-79 -0.47292786428104705 1106 80-81 -0.6137185708136812 1106 82-83 -0.6589684866815482 1106 84-85 -0.466217244253194 1106 86-87 -0.35484394519790285 1106 88-89 -0.20069681058244626 1106 90-91 -0.2820958283834081 1106 92-93 -0.26057883230921774 1106 94-95 -0.35676333986386766 1106 96-97 -0.2724462575769202 1106 98-99 -0.2910116810069212 1106 100-101 -0.3576651233293582 1107 1 0.18661520539463083 1107 2 0.18144769005673567 1107 3 0.13022609346482739 1107 4 0.1150802583720889 1107 5 0.08849170181203903 1107 6 0.10167682720636861 1107 7 0.09333618420948397 1107 8 0.15045502029604307 1107 9 0.0773942910984502 1107 10-11 0.17049926628271805 1107 12-13 0.17138677412498993 1107 14-15 0.2670561900133279 1107 16-17 0.22749288299036863 1107 18-19 0.22055354439622477 1107 20-21 0.12845050363835497 1107 22-23 0.25941130996105244 1107 24-25 0.21748758156689263 1107 26-27 0.11511954619063403 1107 28-29 0.2570058562405606 1107 30-31 0.16099433535357832 1107 32-33 0.19816497328476856 1107 34-35 0.25017551203432475 1107 36-37 0.07179568939761083 1107 38-39 0.023329685520984356 1107 40-41 0.17209648549881962 1107 42-43 0.16593257972193953 1107 44-45 0.17985970714484978 1107 46-47 0.19993189744946704 1107 48-49 0.24557175098271244 1107 50-51 0.2380048262455503 1107 52-53 0.178770495012337 1107 54-55 0.10690452796762884 1107 56-57 0.13922688767115687 1107 58-59 0.14747370775258872 1107 60-61 0.10157713428649373 1107 62-63 0.24311720092492806 1107 64-65 0.24650918022293666 1107 66-67 0.18747230022240302 1107 68-69 0.4958718207471833 1107 70-71 0.33227354330228565 1107 72-73 0.45464771429201534 1107 74-75 0.4014653254580125 1107 76-77 0.47949106458694146 1107 78-79 0.6395331162776721 1107 80-81 0.6788417152641166 1107 82-83 0.6493020760141377 1107 84-85 0.7071674501747722 1107 86-87 0.8770606220786838 1107 88-89 0.897700514848129 1107 90-91 0.8421227684670178 1107 92-93 1.0574935165362298 1107 94-95 1.0086405682089143 1107 96-97 1.2404924588911364 1107 98-99 1.119007319950171 1107 100-101 1.3399980023857374 1108 1 0.06560640714940291 1108 2 0.04720493913764301 1108 3 0.06158685403906361 1108 4 0.03295869592341205 1108 5 0.015024484259129167 1108 6 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100-101 -0.3746355237180019 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 0.0 4 12.0 5 26.0 6 42.0 7 138.0 8 309.0 9 564.0 10 998.0 11 1561.0 12 2492.0 13 3641.0 14 5042.0 15 6739.0 16 8567.0 17 12070.0 18 12370.0 19 8699.0 20 7640.0 21 7661.0 22 7944.0 23 8324.0 24 9350.0 25 11150.0 26 13305.0 27 16105.0 28 19543.0 29 24185.0 30 29511.0 31 36805.0 32 45915.0 33 59918.0 34 84593.0 35 128760.0 36 215608.0 37 352283.0 38 308553.0 39 38216.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.46527856148666 18.36780756767214 14.917428431704472 23.24948543913672 2 16.12103931110333 19.26029740481279 39.662753987647214 24.955909296436666 3 19.625985521500088 22.945251492725816 29.567593204671383 27.861169781102706 4 13.444951392135682 16.368543806191926 35.595721755579554 34.59078304609283 5 14.220151439426918 36.633106012972846 33.83788511454689 15.308857433053348 6 33.60653425121318 35.16909302937968 16.49463137020975 14.729741349197392 7 29.626076816010233 32.21708557026241 19.556873082152396 18.599964531574958 8 27.08077087656342 34.75064202767218 18.771055249646825 19.397531846117573 9 26.388278713082126 16.66371095266693 19.683577381264268 37.26443295298668 10-11 25.704128847114966 25.97538168861947 26.34642306307468 21.97406640119088 12-13 26.407854643945377 23.169681482795127 27.066304458810947 23.356159414448545 14-15 23.019847539512366 24.760285843261805 25.32385090363874 26.896015713587097 16-17 22.682607337545218 27.992570424782272 26.307010737952968 23.017811499719542 18-19 22.660393860738065 26.158702393043892 28.681897936919942 22.4990058092981 20-21 23.019847539512366 25.37225052530887 29.18003450110436 22.427867434074408 22-23 22.934971867090137 25.14278056330308 29.113329679010754 22.80891789059603 24-25 22.849727288056233 25.547724758106288 28.989827129044244 22.612720824793243 26-27 22.4249401762177 25.92621459159413 28.79762176319155 22.85122346899662 28-29 22.52871231393339 26.203828039824085 28.755876980909512 22.511582665333016 30-31 22.5507305958158 25.737850720922612 28.72499072984345 22.986427953418133 32-33 22.371157566638004 25.72603181427343 28.81529449699054 23.087516122098023 34-35 23.05543601790814 25.90703879100239 28.475849643923386 22.561675547166086 36-37 23.10029047505166 25.506651905553955 28.44623390093753 22.946823718456855 38-39 22.661754860044752 25.84449670834827 28.467572099048198 23.02617633255878 40-41 23.039064355459466 25.616883847628557 28.59838413467627 22.745667662235707 42-43 22.769355208202473 25.715220483068162 28.36004990168133 23.15537440704804 44-45 22.79499168852997 25.889733713868452 28.47964084507279 22.835633752528793 46-47 23.206769903553393 26.222460320525848 27.745251982084408 22.82551779383635 48-49 23.08621648636884 26.146980347268123 27.699093405559942 23.06770976080309 50-51 23.059010592161478 26.474391528420423 27.62412605263299 22.84247182678511 52-53 23.359148704058985 26.20159366082512 27.60383918831047 22.835418446805424 54-55 22.92885893777441 26.414437798593088 27.618516936172956 23.03818632745955 56-57 23.01292850962753 26.54945964391851 27.50979412191465 22.927817724539313 58-59 22.98851037988833 26.248851306689026 27.43301304270721 23.32962527071544 60-61 22.692973758739473 26.442046743085 27.735770981454312 23.129208516721214 62-63 22.718030051429217 26.502873748528867 27.887519413588706 22.89157678645321 64-65 22.477980019453895 26.492999016557306 27.717465781030842 23.31155518295796 66-67 22.516336971533903 26.566992331296586 27.560612045292103 23.356058651877408 68-69 22.804677014352677 26.23599226677737 27.84346404306744 23.11586667580251 70-71 22.61169195135452 26.421994991428466 27.474224934302804 23.492088122914215 72-73 22.739794766795104 26.50603097575787 27.303197263557266 23.450976993889757 74-75 22.647630601727204 26.918888817235505 27.028887957394897 23.404592623642394 76-77 22.757092341507192 26.481545670971247 27.304742289648054 23.456619697873506 78-79 22.80784308983722 26.5817372542065 27.35613120092863 23.25428845502765 80-81 22.96073349777785 26.788905100467 27.027208581209262 23.22315282054589 82-83 22.770960994084557 26.785145696156103 27.152224235983574 23.29166907377577 84-85 22.818774137596307 26.771176426725496 27.01355165017355 23.396497785504643 86-87 22.903807521669865 26.73725880338872 26.969752032972256 23.38918164196916 88-89 22.65427017092372 26.940143277803024 26.740129372554193 23.665457178719056 90-91 22.76378554159728 26.86191383696427 26.83017352044782 23.544127100990632 92-93 22.844403542153337 26.45416881487664 27.01179094376252 23.6896366992075 94-95 22.57686168926435 26.4016073645348 27.146847340674224 23.874683605526627 96-97 22.890804273407817 26.10771653204787 27.088001999129414 23.9134771954149 98-99 22.56349385482666 26.384813602678403 26.80576603737082 24.245926505124114 100-101 22.710775146307252 26.04480710013381 27.114267442672812 24.13015031088612 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 594.0 1 404.0 2 289.5 3 384.5 4 314.0 5 285.0 6 565.0 7 1211.0 8 1949.5 9 2309.5 10 1948.0 11 1444.0 12 1409.0 13 1519.5 14 1613.0 15 1698.5 16 1786.0 17 1893.5 18 2083.5 19 2283.0 20 2509.0 21 2874.0 22 3437.0 23 4208.0 24 5099.5 25 6267.0 26 7743.0 27 9542.0 28 11322.0 29 13004.5 30 14780.0 31 17284.0 32 21425.0 33 23485.0 34 23457.5 35 24959.5 36 27596.0 37 30203.0 38 32450.5 39 34404.0 40 36932.0 41 40593.5 42 42478.5 43 43645.5 44 45920.0 45 49452.0 46 51711.0 47 53318.0 48 54850.5 49 54785.5 50 55303.0 51 55501.5 52 56679.0 53 58989.0 54 60513.5 55 57876.0 56 52933.0 57 48516.5 58 44711.5 59 39545.5 60 32170.5 61 26987.5 62 22794.0 63 17036.0 64 12097.5 65 8854.5 66 6203.5 67 4874.5 68 3691.5 69 2404.5 70 1720.0 71 1202.5 72 810.5 73 600.0 74 430.5 75 314.0 76 206.5 77 109.5 78 65.0 79 36.5 80 16.5 81 10.5 82 6.5 83 4.5 84 4.0 85 2.0 86 1.0 87 0.5 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.016054836334714453 3 0.006448804552856014 4 0.0 5 0.0 6 0.0 7 0.0 8 3.358752371279174E-4 9 4.030502845535009E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.0152514227675045E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.005441178841472262 26-27 0.021395252605048338 28-29 6.717504742558349E-5 30-31 0.0 32-33 5.374003794046679E-4 34-35 0.01843955051832267 36-37 0.003493102466130341 38-39 0.01756627490179008 40-41 0.014744922909915574 42-43 0.0081281807384956 44-45 0.002854939515587298 46-47 3.358752371279174E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 4.030502845535009E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.3435009485116697E-4 84-85 0.0031572272290024237 86-87 1.679376185639587E-4 88-89 1.0076257113837522E-4 90-91 3.358752371279174E-4 92-93 3.6946276084070915E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1488648.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.71523566932251 #Duplication Level Percentage of deduplicated Percentage of total 1 79.87968272398167 46.90154396329294 2 11.795634422828416 13.85166910011087 3 3.433087984129199 6.047237101849958 4 1.5405680639116734 3.6181926774882323 5 0.8152659940266272 2.3934267486228946 6 0.516326182408985 1.8189728089431094 7 0.3501140954714697 1.438992213873132 8 0.24809199389041653 1.165342391115834 9 0.19339411621814723 1.0219662999727974 >10 1.1200319705250692 12.067386834211808 >50 0.06949795820725031 2.7749489960320015 >100 0.03337319981741233 3.64977284671451 >500 0.0034404380816454093 1.368052866492793 >1k 0.0012614939632699833 1.1144004991118632 >5k 2.293625387763606E-4 0.7680946521673125 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6028 0.40493118588141724 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5379 0.36133458010221353 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3169 0.21287772529167406 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 1630 0.10949532730370108 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 1569 0.10539764941074048 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 1495 0.10042669590124731 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 6.717504742558349E-5 6.717504742558349E-5 9 0.0 0.0 0.0 1.3435009485116697E-4 6.717504742558349E-5 10-11 0.0 0.0 0.0 1.679376185639587E-4 6.717504742558349E-5 12-13 0.0 0.0 0.0 2.6870018970233395E-4 1.3435009485116697E-4 14-15 0.0 0.0 0.0 2.6870018970233395E-4 1.679376185639587E-4 16-17 0.0 0.0 0.0 3.358752371279174E-4 2.0152514227675045E-4 18-19 0.0 0.0 0.0 3.358752371279174E-4 2.0152514227675045E-4 20-21 0.0 0.0 0.0 4.702253319790844E-4 2.6870018970233395E-4 22-23 0.0 0.0 0.0 6.381629505430431E-4 2.6870018970233395E-4 24-25 0.0 0.0 0.0 9.06863140245377E-4 2.6870018970233395E-4 26-27 0.0 0.0 0.0 0.0012763259010860862 3.6946276084070915E-4 28-29 0.0 0.0 0.0 0.0036610400846943 4.030502845535009E-4 30-31 0.0 0.0 0.0 0.012796846534573653 4.030502845535009E-4 32-33 6.717504742558349E-5 0.0 0.0 0.034057749044770824 4.030502845535009E-4 34-35 6.717504742558349E-5 0.0 0.0 0.06650329695132765 4.7022533197908436E-4 36-37 6.717504742558349E-5 0.0 0.0 0.11312277986468258 4.7022533197908436E-4 38-39 6.717504742558349E-5 0.0 0.0 0.18281689156872544 5.038128556918762E-4 40-41 6.717504742558349E-5 0.0 0.0 0.2541567919346951 5.374003794046679E-4 42-43 6.717504742558349E-5 0.0 0.0 0.3299638329544661 5.374003794046679E-4 44-45 6.717504742558349E-5 0.0 0.0 0.4229676861151864 5.709879031174596E-4 46-47 6.717504742558349E-5 0.0 0.0 0.5295744863795875 6.045754268302514E-4 48-49 6.717504742558349E-5 0.0 0.0 0.6388011134935861 6.717504742558348E-4 50-51 6.717504742558349E-5 0.0 0.0 0.7575665973420177 6.717504742558348E-4 52-53 6.717504742558349E-5 0.0 0.0 0.8626955465630559 6.717504742558348E-4 54-55 6.717504742558349E-5 0.0 0.0 0.9775312901370907 6.717504742558348E-4 56-57 6.717504742558349E-5 0.0 0.0 1.11070582165831 6.717504742558348E-4 58-59 6.717504742558349E-5 0.0 0.0 1.2549978235284636 6.717504742558348E-4 60-61 6.717504742558349E-5 0.0 0.0 1.40859356946706 6.717504742558348E-4 62-63 6.717504742558349E-5 0.0 0.0 1.5552031104733959 7.389255216814183E-4 64-65 6.717504742558349E-5 0.0 0.0 1.7060782669912564 7.7251304539421E-4 66-67 6.717504742558349E-5 0.0 0.0 1.8788524889698572 8.061005691070018E-4 68-69 6.717504742558349E-5 0.0 0.0 2.0715441125101437 8.061005691070018E-4 70-71 6.717504742558349E-5 0.0 0.0 2.2744127557354057 8.061005691070018E-4 72-73 6.717504742558349E-5 0.0 0.0 2.485140879509461 8.061005691070018E-4 74-75 6.717504742558349E-5 0.0 0.0 2.687237009689329 8.061005691070018E-4 76-77 6.717504742558349E-5 0.0 0.0 2.9022643364986216 8.061005691070018E-4 78-79 6.717504742558349E-5 0.0 0.0 3.1441616822781477 8.061005691070018E-4 80-81 6.717504742558349E-5 0.0 0.0 3.396807035645767 8.061005691070018E-4 82-83 6.717504742558349E-5 0.0 0.0 3.6554981432816893 8.061005691070018E-4 84-85 6.717504742558349E-5 0.0 0.0 3.923996807841746 8.396880928197935E-4 86-87 6.717504742558349E-5 0.0 0.0 4.196559562771052 8.732756165325853E-4 88-89 6.717504742558349E-5 0.0 0.0 4.489509944594021 8.732756165325853E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2905 0.0 56.0864 1 GTATCAA 5090 0.0 43.20886 1 ATCAACG 6510 0.0 33.5616 3 TCAACGC 6570 0.0 33.2551 4 TATCAAC 6700 0.0 32.61314 2 CAACGCA 6725 0.0 32.559254 5 AACGCAG 6970 0.0 31.414776 6 TGGTATC 1250 0.0 28.881063 2 TAAGGTG 790 0.0 28.858913 5 GTGGTAT 1340 0.0 28.004763 1 ACGCAGA 7870 0.0 27.580828 7 CGCAGAG 7980 0.0 27.14112 8 GTAAGGT 770 0.0 25.907434 4 GCAGAGT 8750 0.0 24.209879 9 GTACATG 4920 0.0 24.04052 1 TACATGG 4860 0.0 23.848616 2 GGACCGA 430 0.0 23.19619 6 GAGTACT 5790 0.0 22.764086 12-13 ACATGGG 4995 0.0 22.060648 3 GTACTTT 5985 0.0 21.90336 14-15 >>END_MODULE