FastQCFastQC Report
Thu 26 May 2016
SRR938084_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938084_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1440478
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55700.38667720020715346No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49480.3434970891606814No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28250.19611545611942705No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA16410.11392051805025832No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA15170.1053122644011224No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29750.056.504451
GTATCAA50550.041.450181
ATCAACG63850.032.9205063
TATCAAC63700.032.7740552
TCAACGC64300.032.6161544
CAACGCA65750.031.8245325
AACGCAG67900.030.8814986
GTGGTAT13150.030.4192451
TGGTATC13250.029.4308682
ACGCAGA77050.027.0215427
CGCAGAG77750.026.778268
GCAGAGT84800.024.2717299
GTACATG51650.024.1560461
TACATGG51700.023.6400132
GAGTACT52250.023.56090412-13
ACATGGG53050.022.5005653
CAGAGTA81850.022.29967510-11
GTACTTT55050.022.05994814-15
AGAGTAC76750.020.25141510-11
ACTTTTT63450.019.40301716-17
Produced by FastQC (version 0.11.5)