##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938084_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1440478 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.880183522414086 33.0 31.0 34.0 30.0 34.0 2 31.99681702879183 33.0 31.0 34.0 30.0 34.0 3 32.02056053615536 34.0 31.0 34.0 30.0 34.0 4 35.535159162444685 37.0 35.0 37.0 33.0 37.0 5 35.535935987915124 37.0 35.0 37.0 33.0 37.0 6 35.55744273775788 37.0 35.0 37.0 33.0 37.0 7 35.4798879260912 37.0 35.0 37.0 33.0 37.0 8 35.466022389790055 37.0 35.0 37.0 33.0 37.0 9 37.08436227418954 39.0 37.0 39.0 33.0 39.0 10-11 36.988554840823674 39.0 37.0 39.0 33.0 39.0 12-13 36.83273191260123 39.0 37.0 39.0 33.0 39.0 14-15 38.00820179134982 40.0 38.0 41.0 33.0 41.0 16-17 37.8164154537591 40.0 38.0 41.0 33.0 41.0 18-19 37.97425542077005 40.0 38.0 41.0 33.0 41.0 20-21 37.968848882107196 40.0 38.0 41.0 33.0 41.0 22-23 37.85062631987438 40.0 38.0 41.0 33.0 41.0 24-25 37.725914245132515 40.0 38.0 41.0 32.0 41.0 26-27 37.540855535454206 40.0 37.5 41.0 32.0 41.0 28-29 37.35041111353315 40.0 37.0 41.0 31.5 41.0 30-31 37.111465430225245 40.0 37.0 41.0 31.0 41.0 32-33 36.809580916890084 39.5 36.0 41.0 30.0 41.0 34-35 36.56470838152335 39.0 36.0 41.0 30.0 41.0 36-37 36.286643391985166 39.0 35.0 41.0 30.0 41.0 38-39 35.980307578456596 39.0 35.0 40.5 28.5 41.0 40-41 35.85728383217238 39.0 35.0 40.0 28.0 41.0 42-43 35.62333197730198 39.0 35.0 40.5 27.0 41.0 44-45 35.285269889578316 38.5 35.0 40.0 26.0 41.0 46-47 34.89478770241545 38.0 34.0 40.0 24.5 41.0 48-49 34.477603962018165 38.0 34.0 40.0 23.5 41.0 50-51 33.73402891262484 37.5 33.0 39.5 22.0 40.5 52-53 33.79333040837833 38.0 33.0 39.5 22.0 40.5 54-55 33.85441013330298 38.0 33.0 40.0 21.0 41.0 56-57 33.518966273695256 38.0 33.0 40.0 18.5 41.0 58-59 33.08820474870147 37.0 32.5 40.0 16.0 41.0 60-61 32.56669765175171 36.5 32.0 40.0 10.5 41.0 62-63 32.00391536698235 36.0 31.0 39.0 7.5 41.0 64-65 31.443845029219467 35.0 30.5 39.0 4.0 40.0 66-67 30.868034777344743 35.0 30.0 38.5 2.0 40.0 68-69 30.178869791832987 35.0 29.0 38.0 2.0 40.0 70-71 29.574811972137027 34.0 28.5 37.0 2.0 39.5 72-73 28.884655649027614 34.0 27.5 36.5 2.0 39.0 74-75 28.199255733166353 34.0 26.0 36.0 2.0 39.0 76-77 27.445991191812716 33.5 25.5 35.0 2.0 37.5 78-79 26.69533793643499 33.0 23.5 35.0 2.0 37.0 80-81 25.978471035309113 32.0 21.0 35.0 2.0 36.5 82-83 25.243906189473215 31.5 19.0 35.0 2.0 36.0 84-85 24.532060538238 31.0 14.0 34.5 2.0 35.5 86-87 23.78358572640471 31.0 7.0 34.0 2.0 35.0 88-89 22.9167488153238 30.0 2.0 34.0 2.0 35.0 90-91 22.02820244391098 29.5 2.0 34.0 2.0 35.0 92-93 21.103310498320695 29.0 2.0 34.0 2.0 35.0 94-95 20.03756843214544 27.5 2.0 34.0 2.0 35.0 96-97 18.79385939944935 25.5 2.0 33.0 2.0 35.0 98-99 17.26380687521781 21.0 2.0 33.0 2.0 34.5 100-101 13.451190507595395 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.16133327802175046 1101 2 0.19578823848046767 1101 3 0.1566808334440708 1101 4 -0.0609677781472584 1101 5 0.09198591153440816 1101 6 0.05747500903780889 1101 7 0.10258345067148866 1101 8 0.0752406135185879 1101 9 0.09692834561294461 1101 10-11 0.10699530088952969 1101 12-13 0.33629558057591424 1101 14-15 0.3745410851284703 1101 16-17 0.38894666706600844 1101 18-19 0.43998741021657395 1101 20-21 0.3569829831570388 1101 22-23 0.25730731172831867 1101 24-25 0.27618009394977605 1101 26-27 0.3097125907025742 1101 28-29 0.4158155107789838 1101 30-31 0.22239309319659384 1101 32-33 0.23879072046502614 1101 34-35 0.2681595098868499 1101 36-37 0.2602066417164224 1101 38-39 0.2554147087507843 1101 40-41 0.24125557400245157 1101 42-43 0.16523343855892136 1101 44-45 0.20608808639394027 1101 46-47 0.374595846986864 1101 48-49 0.47885678309318536 1101 50-51 0.6422798693057601 1101 52-53 0.523729465096352 1101 54-55 0.3589632181911284 1101 56-57 0.3612494966598021 1101 58-59 0.4721918395357392 1101 60-61 0.5476872123912884 1101 62-63 0.2523612757045939 1101 64-65 0.24417317422058105 1101 66-67 0.1283915657004684 1101 68-69 0.3157506665179497 1101 70-71 0.42468053666300776 1101 72-73 0.3348312956866266 1101 74-75 0.12078272255959632 1101 76-77 0.03283070958682188 1101 78-79 0.15531866639731007 1101 80-81 -0.18812094508217925 1101 82-83 -0.3485232166708485 1101 84-85 -0.03320603314964998 1101 86-87 -0.14976098892308798 1101 88-89 -0.21986248433836764 1101 90-91 -0.21254509443335579 1101 92-93 -0.2989718985997243 1101 94-95 -0.2175180876904328 1101 96-97 -0.3602002748309907 1101 98-99 -0.7543365095235011 1101 100-101 -1.371182970152459 1102 1 0.18812020266636154 1102 2 0.1799851362198268 1102 3 0.1944969586741223 1102 4 0.1937716590711389 1102 5 0.11616043035315471 1102 6 0.08625455369340074 1102 7 0.05176503470387672 1102 8 0.2494423592582109 1102 9 0.1774080219227656 1102 10-11 0.12359568091760309 1102 12-13 0.1229855796798418 1102 14-15 0.2164333791056947 1102 16-17 0.24322261478113205 1102 18-19 0.27279372456458617 1102 20-21 0.24707525599605162 1102 22-23 0.3504986285233045 1102 24-25 0.22368699258520763 1102 26-27 0.1765427912167894 1102 28-29 0.024400951613856137 1102 30-31 0.19478792490665597 1102 32-33 0.21730184444286493 1102 34-35 0.09960189335678393 1102 36-37 0.07473616741656741 1102 38-39 -0.0132828334739159 1102 40-41 0.042246886661700955 1102 42-43 0.10959516830703109 1102 44-45 0.2619440571313163 1102 46-47 0.225618349979527 1102 48-49 0.28806557107014186 1102 50-51 0.13256232850559257 1102 52-53 0.0937362545943401 1102 54-55 0.022708447735901416 1102 56-57 0.15879206600803997 1102 58-59 0.08430988719319288 1102 60-61 -0.036842324797014214 1102 62-63 -0.05755374643307931 1102 64-65 -0.14493772321089793 1102 66-67 -0.021668402454373847 1102 68-69 -0.25586503870525235 1102 70-71 -0.5670500041302731 1102 72-73 -0.7091240981157441 1102 74-75 -0.7342893541077373 1102 76-77 -0.8496655969722262 1102 78-79 -0.9815198745154454 1102 80-81 -0.9570761091215054 1102 82-83 -0.9571922338134655 1102 84-85 -0.8017800777374688 1102 86-87 -0.7500215402165402 1102 88-89 -0.799275485710055 1102 90-91 -0.6365314373179416 1102 92-93 -0.8938278486584288 1102 94-95 -1.1799167909078072 1102 96-97 -1.206082116887277 1102 98-99 -1.229201006767493 1102 100-101 -1.2060264211014946 1103 1 0.3998675319081286 1103 2 0.310329713562183 1103 3 0.3836811391481447 1103 4 0.30550051171918824 1103 5 0.39625334758468966 1103 6 0.38414788809526357 1103 7 0.40081961189040527 1103 8 0.3788297154802578 1103 9 0.2525504363906066 1103 10-11 0.3543249275845852 1103 12-13 0.15076808791083351 1103 14-15 0.30154842372778035 1103 16-17 0.35083291225428326 1103 18-19 0.32806795728507865 1103 20-21 0.37840098498672603 1103 22-23 0.3860543844274673 1103 24-25 0.3998220787462472 1103 26-27 0.4095924643146418 1103 28-29 0.4296243737016141 1103 30-31 0.42903968964396455 1103 32-33 0.40367808354334755 1103 34-35 0.019715112580449556 1103 36-37 0.07567112758058414 1103 38-39 0.0377715567205712 1103 40-41 0.36566352049345596 1103 42-43 0.4197737679593203 1103 44-45 0.39115276917917186 1103 46-47 0.43958036610981566 1103 48-49 0.34933495758044586 1103 50-51 0.335722772121926 1103 52-53 0.33047401162490786 1103 54-55 0.4587427028954352 1103 56-57 0.30125606447648323 1103 58-59 0.30394371672323217 1103 60-61 0.3624560655132356 1103 62-63 0.3155373765653806 1103 64-65 0.2239828096273513 1103 66-67 0.1665841569990718 1103 68-69 -0.12135738427543075 1103 70-71 -0.24407775463837922 1103 72-73 0.04769888498596231 1103 74-75 -0.1377243866198654 1103 76-77 -0.23081268178869507 1103 78-79 -0.19686206993547728 1103 80-81 -0.3404835157868824 1103 82-83 -0.5389570045144865 1103 84-85 -0.7992729375357399 1103 86-87 -1.3456980172866402 1103 88-89 -1.0558352330036804 1103 90-91 -1.0602542706034086 1103 92-93 -1.0420296086096315 1103 94-95 -1.089643604452263 1103 96-97 -1.2627507202571415 1103 98-99 -1.5656135868791097 1103 100-101 -1.3848369959461682 1104 1 0.19490750388621336 1104 2 0.2621786787988114 1104 3 0.15353683366688387 1104 4 0.17319112135998438 1104 5 0.21660670103496926 1104 6 0.24598119682475783 1104 7 0.20519951843814965 1104 8 0.2604640876246904 1104 9 0.21466422887918668 1104 10-11 0.2234407079574936 1104 12-13 0.3035298820121568 1104 14-15 0.36670811851318774 1104 16-17 0.3346478194290299 1104 18-19 0.41804631119833147 1104 20-21 0.480057167243487 1104 22-23 0.5850817126781251 1104 24-25 0.4763355190560148 1104 26-27 0.3823200352702685 1104 28-29 0.3500129661980296 1104 30-31 0.3469644590425034 1104 32-33 0.21167925182883351 1104 34-35 0.27785937996544163 1104 36-37 0.1976117196283056 1104 38-39 0.13124392026053755 1104 40-41 0.25618834417917924 1104 42-43 0.30656515756824376 1104 44-45 0.20277943212087024 1104 46-47 -0.12243982596944392 1104 48-49 -0.16283018550013395 1104 50-51 -0.23835435905991886 1104 52-53 -0.21139087467625473 1104 54-55 -0.32601286458284306 1104 56-57 -0.11885871110740709 1104 58-59 -0.21481525507970645 1104 60-61 -0.06929420922962493 1104 62-63 -0.2618954007212011 1104 64-65 -0.30171682565381275 1104 66-67 -0.23692487948815355 1104 68-69 -0.1724537366108656 1104 70-71 -0.3599046896509108 1104 72-73 -0.17444970209231414 1104 74-75 -0.3609692995778353 1104 76-77 -0.35739334613466056 1104 78-79 -0.4610205207719815 1104 80-81 -0.3321420208576562 1104 82-83 -0.2666454236199769 1104 84-85 -0.5778489731853789 1104 86-87 -0.7335275301465138 1104 88-89 -0.7597380896416119 1104 90-91 -0.6985688494624185 1104 92-93 -0.6722116928880979 1104 94-95 -1.1462551849763933 1104 96-97 -1.3852302157355858 1104 98-99 -1.6103203488076083 1104 100-101 -1.7425913586300297 1105 1 0.23600541181917833 1105 2 0.29098204862518884 1105 3 0.3813831406307244 1105 4 0.24831000022919625 1105 5 0.2628211756054455 1105 6 0.15760971166827886 1105 7 0.3361050084804731 1105 8 0.37304765469448853 1105 9 0.42953338672418795 1105 10-11 0.3886466473770298 1105 12-13 0.37508239480482786 1105 14-15 0.3395765927418637 1105 16-17 0.5714593021727481 1105 18-19 0.450288120368846 1105 20-21 0.4654654772031819 1105 22-23 0.6087378536639392 1105 24-25 0.526249061696582 1105 26-27 0.5101298994517833 1105 28-29 0.5385013195874606 1105 30-31 0.4469418393845146 1105 32-33 0.4221732651593513 1105 34-35 0.42853646172796545 1105 36-37 0.6663679400342559 1105 38-39 0.4975195181735046 1105 40-41 0.4814529941776726 1105 42-43 0.5285024558762927 1105 44-45 0.6420237847462573 1105 46-47 0.528201566999364 1105 48-49 0.6499094572098087 1105 50-51 0.3991771827964996 1105 52-53 0.52087430814084 1105 54-55 0.634928766646361 1105 56-57 0.6381908309701814 1105 58-59 0.6883766299923266 1105 60-61 0.6973337526889267 1105 62-63 0.6840325581813929 1105 64-65 0.7630487844234928 1105 66-67 0.8516471666358392 1105 68-69 0.472601103493286 1105 70-71 0.4159111260139561 1105 72-73 0.22753580122020978 1105 74-75 0.19261290025930577 1105 76-77 -0.136149968667862 1105 78-79 0.09463162910084932 1105 80-81 0.18842678814195324 1105 82-83 0.12518309926609206 1105 84-85 -0.12219732597161581 1105 86-87 0.09349768323226115 1105 88-89 -0.07014212699923306 1105 90-91 -0.36084730247887364 1105 92-93 -0.33895325575566915 1105 94-95 -0.4408111359570803 1105 96-97 -0.3700524252237827 1105 98-99 -0.6187084719791862 1105 100-101 -0.9325575296748578 1106 1 0.24668818980577356 1106 2 0.2520407082754019 1106 3 0.33180656026943467 1106 4 0.24862949964326475 1106 5 0.3956400669756448 1106 6 0.2981960870635092 1106 7 0.42000179579778063 1106 8 0.16741606294619515 1106 9 0.4245087333091888 1106 10-11 0.5978214765719798 1106 12-13 0.6044354361296982 1106 14-15 0.4662453967748519 1106 16-17 0.07214749727744163 1106 18-19 0.34275556238356586 1106 20-21 0.5191369566291826 1106 22-23 0.5248012671116626 1106 24-25 0.4426286579554528 1106 26-27 0.3512231623257307 1106 28-29 0.3649683636098331 1106 30-31 0.2844625681527262 1106 32-33 0.3033924483906105 1106 34-35 0.21353215014418225 1106 36-37 0.1999253283200133 1106 38-39 0.18132896828073797 1106 40-41 0.1824369197088771 1106 42-43 -0.0716539772278253 1106 44-45 0.34223549456561386 1106 46-47 0.2576242569854301 1106 48-49 0.10026709755738494 1106 50-51 0.04949004900473852 1106 52-53 -0.08455054869246226 1106 54-55 0.10996524927841733 1106 56-57 -0.28899481035026753 1106 58-59 -0.25856988425801575 1106 60-61 0.11158298434258285 1106 62-63 0.053495489616160796 1106 64-65 0.03432258104940544 1106 66-67 -0.05515518594116031 1106 68-69 -0.24428261640854387 1106 70-71 -0.44910258261142033 1106 72-73 -0.341539087673592 1106 74-75 -0.32141356623194994 1106 76-77 -0.6309838068618596 1106 78-79 -0.4987511953181958 1106 80-81 -0.6742216298384562 1106 82-83 -0.6323958911599803 1106 84-85 -0.72371024966764 1106 86-87 -0.6365568196089626 1106 88-89 -0.6840641204986895 1106 90-91 -0.8018781026646025 1106 92-93 -1.2274181108201176 1106 94-95 -1.0560205241543947 1106 96-97 -1.0343735166072463 1106 98-99 -1.5053994972516982 1106 100-101 -1.3232118954298269 1107 1 -0.04456015210701736 1107 2 -0.006847044798398372 1107 3 0.0858650709724671 1107 4 0.10850034124167962 1107 5 0.013621845256430731 1107 6 -0.059460929231384796 1107 7 -0.003109607380068269 1107 8 0.03156750766242311 1107 9 0.05088033144158999 1107 10-11 0.056709026821650355 1107 12-13 0.0448725829621921 1107 14-15 0.035484988854975086 1107 16-17 0.08078983316230648 1107 18-19 0.08684323919549541 1107 20-21 0.12607344022680422 1107 22-23 0.15513756979014914 1107 24-25 0.2499807281812778 1107 26-27 0.1779657257792593 1107 28-29 0.10122195866741635 1107 30-31 -0.11096841282643055 1107 32-33 0.08797193313620966 1107 34-35 0.02336577351601221 1107 36-37 0.13538111468941594 1107 38-39 -0.25011850635610955 1107 40-41 -0.26597121516104494 1107 42-43 -0.18049142991909406 1107 44-45 -0.06271199426892338 1107 46-47 -0.17903630467512954 1107 48-49 -0.20802354129804002 1107 50-51 -0.29420455465663764 1107 52-53 -0.3850943201664663 1107 54-55 -0.32694934959631183 1107 56-57 -0.5149718141511883 1107 58-59 -0.4064454029807081 1107 60-61 -0.37767443597795847 1107 62-63 -0.49630645262764617 1107 64-65 -0.6817768879570387 1107 66-67 -0.6784443644628944 1107 68-69 -0.5474793274125638 1107 70-71 -0.7377846923068212 1107 72-73 -0.6331585977316401 1107 74-75 -0.8084652742138907 1107 76-77 -0.9975285762305539 1107 78-79 -1.0079969329692489 1107 80-81 -1.1765921367770709 1107 82-83 -1.033550568892732 1107 84-85 -1.156448699847953 1107 86-87 -1.171061766489327 1107 88-89 -0.8569174299799052 1107 90-91 -0.9755477792412606 1107 92-93 -1.076269010750753 1107 94-95 -1.0921313310293215 1107 96-97 -1.0932163441377831 1107 98-99 -1.2197293257841864 1107 100-101 -1.1307505638488937 1108 1 0.10276398637304496 1108 2 0.047072377504761675 1108 3 0.01773707941103453 1108 4 0.10056099012234654 1108 5 0.07168591732921925 1108 6 0.14009176702997195 1108 7 0.13757131595303917 1108 8 0.026337354381702482 1108 9 0.0030883235337455517 1108 10-11 -0.02811283118884944 1108 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-0.21516748335243108 2316 60-61 -0.28488925897153905 2316 62-63 -0.3597924094284224 2316 64-65 -0.07192843796125814 2316 66-67 -0.11718929426606906 2316 68-69 0.047246901127753205 2316 70-71 -0.0737731219068003 2316 72-73 0.05852589609211023 2316 74-75 0.16141488128492298 2316 76-77 0.22072719938496377 2316 78-79 0.3895463697703825 2316 80-81 0.4360131968016283 2316 82-83 0.3837873956676461 2316 84-85 0.4577029958405596 2316 86-87 0.3253894791113936 2316 88-89 0.05370122987793735 2316 90-91 0.2079076605336212 2316 92-93 0.2330971475543997 2316 94-95 0.22938048856751436 2316 96-97 0.2461113228432552 2316 98-99 0.3663074096608021 2316 100-101 0.3722748772294775 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5154.0 3 1777.0 4 1665.0 5 1838.0 6 2240.0 7 2736.0 8 3279.0 9 4208.0 10 5244.0 11 6322.0 12 7769.0 13 9486.0 14 10749.0 15 13066.0 16 15150.0 17 18220.0 18 18134.0 19 16317.0 20 16574.0 21 17764.0 22 19357.0 23 21644.0 24 23657.0 25 26510.0 26 29756.0 27 33734.0 28 38536.0 29 44022.0 30 51197.0 31 60232.0 32 73079.0 33 90126.0 34 115504.0 35 150011.0 36 187115.0 37 194343.0 38 97770.0 39 6193.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.1958779944189 17.908463834774953 14.467132824351062 23.428525346455086 2 15.805832768411813 19.03873509440672 40.21683262394008 24.938599513241392 3 19.592185514082374 22.98602748336175 29.352088507438197 28.069698495117684 4 13.21721745936744 15.998048052118676 35.82470840693108 34.960026081582804 5 14.264617396536552 36.521805667143155 33.788819443719845 15.424757492600438 6 33.487649597502376 34.9295733497319 16.738155328189304 14.844621724576424 7 29.520750421239217 32.20917108883818 19.658711046237915 18.611367443684678 8 27.028897156136345 34.610866219957416 18.89260567661168 19.467630947294563 9 26.55428376404873 16.709380232759393 19.700650539080655 37.035685464111225 10-11 25.72793352947124 25.916959790570022 26.34970483757814 22.005401842380593 12-13 26.467623018069258 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22.979181121084974 38-39 22.67605071274643 25.887012890039607 28.304672400589716 23.132263996624243 40-41 23.080489871905304 25.609491789157797 28.459112506599237 22.85090583233766 42-43 22.810462306457644 25.71460923695937 28.221676743587494 23.253251712995493 44-45 22.844950618407136 25.92757597875977 28.364361914537657 22.86311148829544 46-47 23.28209135345853 26.22821868569791 27.626042941855722 22.863647018987837 48-49 23.096393290514712 26.204551515690284 27.58724386612143 23.11181132767357 50-51 23.10690057683378 26.495838107287817 27.4889827144355 22.9082786014429 52-53 23.3391467263121 26.21415484226238 27.49159673639845 22.955101695027068 54-55 22.950511093754177 26.46178231465927 27.537696051243778 23.05001054034277 56-57 23.056157476061003 26.57464082387862 27.416754712369183 22.952446987691193 58-59 23.053226629325128 26.184366216602466 27.384491105163043 23.377916048909363 60-61 22.82215167587697 26.347427863429623 27.70598518063746 23.12443528005595 62-63 22.814611119832076 26.452492119875142 27.826078802637777 22.906817957655008 64-65 22.56799623579089 26.420719818178977 27.63929570397482 23.371988242055313 66-67 22.650770511852667 26.432213083387797 27.506394599020616 23.410621805738923 68-69 22.875239449877473 26.17385580732165 27.810468583655062 23.140436159145814 70-71 22.736152686422074 26.34086672758398 27.427549557828552 23.4954310281654 72-73 22.8405943874521 26.397840611752578 27.266340063361877 23.49522493743345 74-75 22.71382527671772 26.74194208210354 27.134814740729396 23.409417900449338 76-77 22.950718360026123 26.35279478349208 27.17935756070412 23.517129295777682 78-79 22.960880626820984 26.505149442143765 27.180840131208008 23.35312979982724 80-81 23.162384170892917 26.610184732865243 26.913113717631298 23.314317378610543 82-83 23.034553201180984 26.619789800549388 26.989784440018997 23.355872558250635 84-85 23.158699089218025 26.56119917427619 26.87952500744693 23.40057672905885 86-87 23.238162959835535 26.49815130065174 26.800672052297585 23.46301368721514 88-89 23.05518417824999 26.63954541333686 26.584246956240232 23.72102345217291 90-91 23.08738578665209 26.630384355955393 26.622912901256214 23.659316956136305 92-93 23.239732402013967 26.142844685959375 26.819934153136167 23.797488758890488 94-95 23.07670662682809 26.030486167489457 26.90316217822656 23.989645027455893 96-97 23.41034820299023 25.692032688188775 26.844059999208014 24.053559109612976 98-99 23.141978134340643 25.98222850787957 26.471805886181592 24.40398747159819 100-101 24.058885727374918 24.75889497323913 26.502505073059705 24.679714226326244 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 549.0 1 366.0 2 267.0 3 364.5 4 291.5 5 285.5 6 518.5 7 1044.5 8 1725.0 9 2065.0 10 1788.5 11 1384.0 12 1306.0 13 1395.5 14 1527.0 15 1635.5 16 1782.5 17 1926.5 18 2069.0 19 2268.0 20 2537.0 21 2943.5 22 3541.5 23 4330.0 24 5161.0 25 6288.5 26 7795.5 27 9572.5 28 11232.0 29 12738.5 30 14425.5 31 17007.0 32 20540.0 33 22235.0 34 22827.0 35 24475.5 36 26647.5 37 29062.0 38 31303.0 39 33293.0 40 35416.5 41 38345.5 42 40405.0 43 41753.0 44 43762.0 45 46812.0 46 49029.0 47 50409.5 48 51874.0 49 52389.0 50 52874.0 51 53116.0 52 54105.0 53 56017.0 54 56927.0 55 54344.0 56 50242.5 57 46558.5 58 43361.0 59 38571.5 60 31806.5 61 27034.0 62 23114.5 63 18205.0 64 13497.5 65 9998.5 66 7446.5 67 5793.0 68 4343.5 69 2995.0 70 2218.0 71 1585.0 72 1075.5 73 828.0 74 639.0 75 470.0 76 336.5 77 199.0 78 116.5 79 76.0 80 45.0 81 25.0 82 18.5 83 12.0 84 10.5 85 9.0 86 4.0 87 4.0 88 2.5 89 1.0 90 4.0 91 3.5 92 2.0 93 2.5 94 2.5 95 2.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1424527136131201 2 0.05206605029719301 3 0.15453203728206885 4 0.13294198175883282 5 0.20083611134637253 6 0.2050708167705442 7 0.17070722357439685 8 0.07379494862122157 9 0.1266940557231697 10-11 0.10583292490409432 12-13 0.15508740848523891 14-15 0.14141139260717622 16-17 0.14047420370182676 18-19 0.1387386686919203 20-21 0.14037007160123238 22-23 0.2043418920663835 24-25 0.223085670173373 26-27 0.15474030148325765 28-29 0.1324213212558609 30-31 0.10461805039715982 32-33 0.1303733899441713 34-35 0.0988907848644686 36-37 0.09215690902603164 38-39 0.05557183101720402 40-41 0.06372884556376425 42-43 0.06716520488337899 44-45 0.03950077682547043 46-47 0.051406546993428566 48-49 0.04206936864013196 50-51 0.056127202220374074 52-53 0.02863632766345616 54-55 0.053558610405712546 56-57 0.0285669062630599 58-59 0.055155302614826464 60-61 0.0419999472397357 62-63 0.05414869230908073 64-65 0.04168755093795254 66-67 0.041028047634188096 68-69 0.06806768308853034 70-71 0.05702968042552542 72-73 0.05630075572136471 74-75 0.04849084817678576 76-77 0.024818150641662005 78-79 0.022353690927594868 80-81 0.02579005024720961 82-83 0.022353690927594868 84-85 0.01992394191372586 86-87 0.0285669062630599 88-89 0.0705668535027956 90-91 0.1158296065611554 92-93 0.08535361178719843 94-95 0.08118832776342297 96-97 0.07351726301963653 98-99 0.06032719694434764 100-101 0.13863453659132594 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1440478.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.7495726677638 #Duplication Level Percentage of deduplicated Percentage of total 1 82.06263776673715 50.67332814085513 2 10.520151168576666 12.992296781197698 3 3.146384900089068 5.828637690864139 4 1.309331283829412 3.2340258902800305 5 0.7354553421193327 2.270702654604641 6 0.4663328540113237 1.7277512673682751 7 0.3021999348913749 1.306250178783791 8 0.21863300818300052 1.0800395861094392 9 0.1694111508806893 0.9414959554832958 >10 0.9757786774287059 11.036722174023836 >50 0.05942627311705642 2.4818513213106552 >100 0.030088200361834037 3.49469448926996 >500 0.0028171890367998716 1.182233649320981 >1k 0.0012395631761919434 1.362386852269305 >5k 1.1268756147199486E-4 0.3875833682588502 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5570 0.38667720020715346 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4948 0.3434970891606814 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2825 0.19611545611942705 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 1641 0.11392051805025832 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 1517 0.1053122644011224 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 6.942140039625735E-5 10-11 0.0 0.0 0.0 1.0413210059438604E-4 6.942140039625735E-5 12-13 0.0 0.0 0.0 1.388428007925147E-4 1.388428007925147E-4 14-15 0.0 0.0 0.0 1.388428007925147E-4 2.0826420118877207E-4 16-17 0.0 0.0 0.0 1.388428007925147E-4 2.776856015850294E-4 18-19 0.0 0.0 0.0 1.735535009906434E-4 3.4710700198128675E-4 20-21 0.0 0.0 0.0 2.0826420118877207E-4 4.1652840237754415E-4 22-23 0.0 0.0 0.0 2.776856015850294E-4 4.1652840237754415E-4 24-25 0.0 0.0 0.0 5.206605029719302E-4 4.1652840237754415E-4 26-27 0.0 0.0 0.0 0.0012495852071326323 4.1652840237754415E-4 28-29 0.0 0.0 0.0 0.0046859445267473715 4.1652840237754415E-4 30-31 0.0 0.0 0.0 0.014266097781430885 4.512391025756728E-4 32-33 0.0 0.0 0.0 0.034085907594562356 5.553712031700588E-4 34-35 0.0 0.0 0.0 0.06386768836455677 5.553712031700588E-4 36-37 0.0 0.0 0.0 0.11471886415481528 5.553712031700588E-4 38-39 0.0 0.0 0.0 0.1854245604584034 6.247926035663162E-4 40-41 0.0 0.0 0.0 0.2600872765845782 6.247926035663162E-4 42-43 0.0 0.0 0.0 0.3360342886180837 6.595033037644449E-4 44-45 0.0 0.0 0.0 0.42982260055342736 6.942140039625735E-4 46-47 0.0 0.0 0.0 0.5406191555858542 6.942140039625735E-4 48-49 0.0 0.0 0.0 0.6575247938531515 7.636354043588308E-4 50-51 0.0 0.0 0.0 0.7764436527319404 7.636354043588308E-4 52-53 0.0 0.0 0.0 0.8873096291647633 7.983461045569596E-4 54-55 0.0 0.0 0.0 1.0089359226590062 8.330568047550883E-4 56-57 0.0 0.0 0.0 1.1499654975640032 8.330568047550883E-4 58-59 0.0 0.0 0.0 1.2970347343034743 8.330568047550883E-4 60-61 0.0 0.0 0.0 1.4568427980156586 8.330568047550883E-4 62-63 0.0 0.0 0.0 1.614429376915163 8.330568047550883E-4 64-65 0.0 0.0 0.0 1.7726060377180353 9.024782051513456E-4 66-67 0.0 0.0 0.0 1.9503595334326522 9.024782051513456E-4 68-69 0.0 0.0 0.0 2.140470038417803 9.024782051513456E-4 70-71 0.0 0.0 0.0 2.3419309423677417 9.024782051513456E-4 72-73 0.0 0.0 0.0 2.541378625706189 0.0010413210059438602 74-75 0.0 0.0 0.0 2.739021352634334 0.0010413210059438602 76-77 0.0 0.0 0.0 2.9619681800069144 0.0010413210059438602 78-79 0.0 6.942140039625735E-5 0.0 3.205081924194608 0.0010413210059438602 80-81 0.0 6.942140039625735E-5 0.0 3.460101438550259 0.0011801638067363749 82-83 0.0 6.942140039625735E-5 0.0 3.7182449159237416 0.0013190066075288898 84-85 0.0 6.942140039625735E-5 0.0 3.9761801290960364 0.0013190066075288898 86-87 0.0 6.942140039625735E-5 0.0 4.24265417451707 0.0014231387081232757 88-89 0.0 6.942140039625735E-5 0.0 4.530405879159557 0.0014578494083214045 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2975 0.0 56.50445 1 GTATCAA 5055 0.0 41.45018 1 ATCAACG 6385 0.0 32.920506 3 TATCAAC 6370 0.0 32.774055 2 TCAACGC 6430 0.0 32.616154 4 CAACGCA 6575 0.0 31.824532 5 AACGCAG 6790 0.0 30.881498 6 GTGGTAT 1315 0.0 30.419245 1 TGGTATC 1325 0.0 29.430868 2 ACGCAGA 7705 0.0 27.021542 7 CGCAGAG 7775 0.0 26.77826 8 GCAGAGT 8480 0.0 24.271729 9 GTACATG 5165 0.0 24.156046 1 TACATGG 5170 0.0 23.640013 2 GAGTACT 5225 0.0 23.560904 12-13 ACATGGG 5305 0.0 22.500565 3 CAGAGTA 8185 0.0 22.299675 10-11 GTACTTT 5505 0.0 22.059948 14-15 AGAGTAC 7675 0.0 20.251415 10-11 ACTTTTT 6345 0.0 19.403017 16-17 >>END_MODULE