FastQCFastQC Report
Thu 26 May 2016
SRR938084_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938084_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1440478
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57140.39667388186421454No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50940.353632613618535No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30330.21055510740184855No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA15590.1082279632177652No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA15380.10677011380944382No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA15030.10434036479557482No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28250.055.3231961
GTATCAA50500.041.6717871
TGGTATC12600.035.435772
GTGGTAT13400.034.387191
ATCAACG63600.032.7093123
TCAACGC64250.032.3011134
TATCAAC64500.032.2551422
CAACGCA64400.032.1521345
AACGCAG66650.031.1379816
ACGCAGA76950.026.9083487
CGCAGAG77850.026.5362668
TACATGG49400.024.518722
TTACACG1551.4134421E-624.5113624
GTACATG50950.024.1482331
GCAGAGT85250.024.121419
ACATGGG50100.023.3212473
TAAGGTG8250.023.0258275
GGACCGA4900.022.2915846
GTAAGGT8100.022.279624
GAGTACT54850.022.16909212-13
Produced by FastQC (version 0.11.5)