##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938084_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1440478 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.52701811482022 34.0 31.0 34.0 31.0 34.0 2 32.73967599644007 34.0 31.0 34.0 31.0 34.0 3 32.792391831044974 34.0 31.0 34.0 31.0 34.0 4 36.248076680102024 37.0 35.0 37.0 35.0 37.0 5 36.2217409776477 37.0 37.0 37.0 35.0 37.0 6 36.25121383318593 37.0 37.0 37.0 35.0 37.0 7 36.20598717925577 37.0 37.0 37.0 35.0 37.0 8 36.190712388526585 37.0 37.0 37.0 35.0 37.0 9 37.88161638011827 39.0 38.0 39.0 35.0 39.0 10-11 37.86125369495404 39.0 38.0 39.0 35.0 39.0 12-13 37.78094215947762 39.0 38.0 39.0 35.0 39.0 14-15 39.06952449117585 40.0 38.5 41.0 36.0 41.0 16-17 38.951733035839496 40.0 38.0 41.0 35.5 41.0 18-19 39.19116015655914 40.5 39.0 41.0 36.0 41.0 20-21 39.1675898555896 40.0 39.0 41.0 36.0 41.0 22-23 39.07721256416273 40.0 39.0 41.0 35.5 41.0 24-25 38.98037179325196 40.0 38.5 41.0 35.0 41.0 26-27 38.809663875463556 40.0 38.0 41.0 35.0 41.0 28-29 38.689432952117286 40.0 38.0 41.0 34.5 41.0 30-31 38.45452342902842 40.0 38.0 41.0 34.0 41.0 32-33 38.24541575782483 40.0 38.0 41.0 34.0 41.0 34-35 38.09731422486147 40.0 38.0 41.0 34.0 41.0 36-37 38.09965858555285 40.0 38.0 41.0 33.5 41.0 38-39 37.9583398705152 40.0 38.0 41.0 33.0 41.0 40-41 37.77516317500163 40.0 37.0 41.0 33.0 41.0 42-43 37.5514541006527 40.0 37.0 41.0 33.0 41.0 44-45 37.280307994984994 40.0 37.0 41.0 32.0 41.0 46-47 36.89164568983351 40.0 36.0 41.0 31.0 41.0 48-49 36.48519137397447 39.0 36.0 41.0 30.0 41.0 50-51 36.09654434153107 39.0 35.0 41.0 29.5 41.0 52-53 35.56731654353624 38.5 35.0 40.0 28.0 41.0 54-55 35.24560180717789 38.0 34.5 40.0 27.0 41.0 56-57 34.8892957754301 38.0 34.0 40.0 26.0 41.0 58-59 34.44392208697391 37.5 34.0 40.0 26.0 41.0 60-61 33.95997613292254 37.0 33.0 40.0 24.5 41.0 62-63 33.48653502517914 36.5 33.0 39.0 23.0 41.0 64-65 32.991728787249784 36.0 32.0 39.0 22.0 40.0 66-67 32.440821727232205 35.0 31.5 38.5 20.5 40.0 68-69 31.86541932608481 35.0 31.0 38.0 18.5 40.0 70-71 31.254450605979404 35.0 30.5 37.0 14.5 39.5 72-73 30.67168571821298 34.0 30.0 37.0 9.0 39.0 74-75 30.078074430848652 34.0 29.0 36.0 7.0 39.0 76-77 28.75962874823496 32.5 28.0 35.0 6.5 37.0 78-79 29.429572683512 34.0 29.0 35.0 2.0 37.0 80-81 29.11322179165527 34.0 29.0 35.0 2.0 37.0 82-83 28.561336931213113 34.0 28.5 35.0 2.0 36.0 84-85 27.99137855628479 33.0 27.0 35.0 2.0 36.0 86-87 27.369269089843787 33.0 26.0 35.0 2.0 35.5 88-89 26.642690134802475 32.5 25.0 35.0 2.0 35.0 90-91 26.12337432435622 32.0 24.0 34.5 2.0 35.0 92-93 25.32709524199606 32.0 21.0 34.0 2.0 35.0 94-95 24.523546003479403 31.0 16.5 34.0 2.0 35.0 96-97 23.515784690915098 31.0 2.0 34.0 2.0 35.0 98-99 22.12900127596534 30.5 2.0 34.0 2.0 35.0 100-101 17.80686063931556 23.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.09546967011327467 1101 2 0.08497260900259818 1101 3 0.09306141711358862 1101 4 0.014427641667850821 1101 5 0.06911857643207497 1101 6 0.03363888645997548 1101 7 -0.11093205663941319 1101 8 -0.08981960530604027 1101 9 -0.14291496600566234 1101 10-11 0.05605825424197519 1101 12-13 0.15022796138610062 1101 14-15 0.19288444800208993 1101 16-17 0.20010924786937068 1101 18-19 0.13882294967184095 1101 20-21 0.1311951319697755 1101 22-23 0.026314480017340713 1101 24-25 0.11266313309662479 1101 26-27 0.043140581657034716 1101 28-29 0.15408890589039714 1101 30-31 0.1685454750105535 1101 32-33 0.03207407219078817 1101 34-35 0.06420749806554227 1101 36-37 0.17610449199803213 1101 38-39 0.08482236814725752 1101 40-41 0.10619592124688637 1101 42-43 0.052633086255411854 1101 44-45 0.17111826420542542 1101 46-47 0.07665348824261287 1101 48-49 0.13019730876031588 1101 50-51 0.1208897230945496 1101 52-53 0.019338498830734352 1101 54-55 -0.03289137668839004 1101 56-57 0.2269015236565508 1101 58-59 0.2233187371738552 1101 60-61 0.07160442243041842 1101 62-63 0.13883784513762976 1101 64-65 -0.059160994733701955 1101 66-67 -0.1912481275179303 1101 68-69 0.0027555995907881936 1101 70-71 -0.024033645256054115 1101 72-73 -0.14400520343979295 1101 74-75 0.01047332198602291 1101 76-77 -0.10947541053586818 1101 78-79 -0.009055350805386553 1101 80-81 0.04711619013945878 1101 82-83 -0.04948433478883629 1101 84-85 -0.1360869320659326 1101 86-87 -0.1689701515956763 1101 88-89 -0.09078070917688308 1101 90-91 -0.2465645032869226 1101 92-93 -0.47259458572792923 1101 94-95 -0.7280313371356222 1101 96-97 -0.5187707772495678 1101 98-99 -0.34726134534231434 1101 100-101 -0.9235301129001918 1102 1 0.08814137778115594 1102 2 0.09418093745346567 1102 3 0.04251929120803766 1102 4 0.11606562241829721 1102 5 0.03283784275271984 1102 6 0.013874669297138098 1102 7 0.04191321403293813 1102 8 0.14316931322228044 1102 9 0.015770881280033677 1102 10-11 0.11486208485145966 1102 12-13 0.1593242803523509 1102 14-15 0.10954852983449825 1102 16-17 0.12315183904508586 1102 18-19 0.21388194133374583 1102 20-21 0.0995070743635793 1102 22-23 0.11202700968549095 1102 24-25 0.18803029444313069 1102 26-27 0.07308473774885726 1102 28-29 0.011099895456858633 1102 30-31 0.16708676687625967 1102 32-33 0.08101072881681404 1102 34-35 0.039833372601137285 1102 36-37 0.08548049003125868 1102 38-39 0.04337713561557166 1102 40-41 0.03530454402422123 1102 42-43 -0.04307557190543321 1102 44-45 -0.14197035999643504 1102 46-47 -0.1353997087952834 1102 48-49 -0.12467567822820058 1102 50-51 -0.09088402463942202 1102 52-53 0.05820808762381091 1102 54-55 0.15226661986975643 1102 56-57 0.12015102794278221 1102 58-59 0.12103368624687505 1102 60-61 -0.05984990871786522 1102 62-63 0.03841847013906374 1102 64-65 0.052287580850304494 1102 66-67 -0.0699932651574855 1102 68-69 0.044803960116574615 1102 70-71 -0.1491870120888379 1102 72-73 -0.06491702957350043 1102 74-75 -0.32450023228745195 1102 76-77 -0.3692242565509716 1102 78-79 -0.20202902455564953 1102 80-81 -0.2735922382583347 1102 82-83 -0.41357087733750575 1102 84-85 -0.5509011341792913 1102 86-87 -0.5898834137484208 1102 88-89 -0.6553567618937137 1102 90-91 -1.0182920456724567 1102 92-93 -0.750339335067931 1102 94-95 -0.6919395835610835 1102 96-97 -0.7157095867553274 1102 98-99 -1.0073596609494295 1102 100-101 -1.2715553569659868 1103 1 0.13069653486559218 1103 2 0.12883178548459995 1103 3 0.12234151521114 1103 4 0.12108546806082643 1103 5 0.03998027057713216 1103 6 0.09567923145751678 1103 7 0.08028442438339312 1103 8 0.13148008507641862 1103 9 0.17265274461112057 1103 10-11 0.13653924755902125 1103 12-13 0.16669599602651886 1103 14-15 0.21258364426694243 1103 16-17 0.24219063321361034 1103 18-19 0.2812870039204256 1103 20-21 0.2568806265666339 1103 22-23 0.22775559965426595 1103 24-25 0.14017508192891626 1103 26-27 0.3031019120766274 1103 28-29 0.2656121912960643 1103 30-31 0.2543243936802284 1103 32-33 0.2976103900050546 1103 34-35 0.07458300362578996 1103 36-37 0.1804671557184392 1103 38-39 0.30719117203894797 1103 40-41 0.2583991174709084 1103 42-43 0.06411343697001115 1103 44-45 -0.0015112389035181195 1103 46-47 0.0610240129249533 1103 48-49 0.06765870582226796 1103 50-51 -0.09202267255345475 1103 52-53 -0.002958898487577244 1103 54-55 0.15821252129379104 1103 56-57 -0.011572893903455395 1103 58-59 0.08434957812095689 1103 60-61 -0.08679679630226644 1103 62-63 -0.07748125093115021 1103 64-65 -0.16645887130978565 1103 66-67 -0.15683798608301203 1103 68-69 -0.06108862631715439 1103 70-71 -0.3326053266227973 1103 72-73 -0.4744571293471047 1103 74-75 -0.3763690952200669 1103 76-77 -0.3797019321509616 1103 78-79 -0.4855036593090105 1103 80-81 -0.5649731298986786 1103 82-83 -0.5842940467634179 1103 84-85 -0.8617019275597748 1103 86-87 -0.7046876175074068 1103 88-89 -1.0920599587896476 1103 90-91 -0.8624881691495325 1103 92-93 -1.0194634443514197 1103 94-95 -0.8345215650978446 1103 96-97 -1.4252806292581717 1103 98-99 -1.7291750641955197 1103 100-101 -2.511207629698319 1104 1 0.06173488563590723 1104 2 0.19206629604546777 1104 3 0.1373646719206576 1104 4 0.03735093342523754 1104 5 0.009485796916735012 1104 6 0.07821738460344818 1104 7 0.12242841874515165 1104 8 0.06864082327623322 1104 9 0.09759145988861917 1104 10-11 0.08273091728132442 1104 12-13 0.15349511071161714 1104 14-15 0.19827135330036327 1104 16-17 0.02090344504080832 1104 18-19 0.08882969422582221 1104 20-21 0.1968739808846678 1104 22-23 0.09616157251384294 1104 24-25 0.12242962953839509 1104 26-27 0.08077792343989643 1104 28-29 0.0322511323468504 1104 30-31 0.03368060911249415 1104 32-33 -0.011116194680560909 1104 34-35 -0.07161935421561338 1104 36-37 -0.09556859974373566 1104 38-39 0.1551071740051384 1104 40-41 0.17055944560674874 1104 42-43 0.20593816345733984 1104 44-45 -0.0065177232054765 1104 46-47 -0.19062007905847622 1104 48-49 -0.1871656307074403 1104 50-51 -0.09671962469343498 1104 52-53 -0.12609237986723798 1104 54-55 -0.09245561842850236 1104 56-57 0.00928507633807385 1104 58-59 -0.08313113167596953 1104 60-61 -0.1785778443622945 1104 62-63 -0.24633871745538727 1104 64-65 -0.22462128733265274 1104 66-67 -0.3890759988547856 1104 68-69 -0.3790776302532386 1104 70-71 -0.5188288702210606 1104 72-73 -0.46571906287018194 1104 74-75 -0.4705935954579026 1104 76-77 -0.7057390368558991 1104 78-79 -0.5727769137724863 1104 80-81 -0.423969060476697 1104 82-83 -0.7122265994407613 1104 84-85 -0.6747894764830527 1104 86-87 -0.833872584932454 1104 88-89 -0.7010287722338191 1104 90-91 -0.7819952563778081 1104 92-93 -0.9432830818125346 1104 94-95 -0.9822647052566538 1104 96-97 -1.1391080923272803 1104 98-99 -1.267606524515351 1104 100-101 -1.5137383858192521 1105 1 0.15868022648901814 1105 2 0.18587403900572497 1105 3 0.12834596295020617 1105 4 0.03702319971390722 1105 5 0.053545874728357035 1105 6 0.11806618475625896 1105 7 0.1292836830935542 1105 8 0.12002715698153565 1105 9 0.16116275202401908 1105 10-11 0.21222494066502406 1105 12-13 0.2645277232318506 1105 14-15 0.2218312350749514 1105 16-17 0.2859452662751423 1105 18-19 0.2796561662629031 1105 20-21 0.2781371128527823 1105 22-23 0.31835975087739143 1105 24-25 0.22336633470875 1105 26-27 0.2222271900603232 1105 28-29 0.19528231731533197 1105 30-31 0.19667057603752625 1105 32-33 0.3809684329998717 1105 34-35 0.3235822623359468 1105 36-37 0.1830802024197027 1105 38-39 0.15598657604191146 1105 40-41 0.17177939916104634 1105 42-43 0.16672648367126897 1105 44-45 0.06996763433592434 1105 46-47 0.15514929090864626 1105 48-49 0.2884518859557019 1105 50-51 0.3039928945332804 1105 52-53 0.05009892212027722 1105 54-55 0.24542527147910675 1105 56-57 0.2091646894916579 1105 58-59 0.2142050266013129 1105 60-61 0.014055687018711183 1105 62-63 0.10152171422822676 1105 64-65 0.17466418280436358 1105 66-67 0.22892924890587096 1105 68-69 0.30879647375698127 1105 70-71 0.2419498994706082 1105 72-73 0.3137563621280606 1105 74-75 0.14082882916837036 1105 76-77 -0.09382350368543158 1105 78-79 0.03219463572435188 1105 80-81 0.0566725335557301 1105 82-83 0.06135087540114981 1105 84-85 -0.1380918460178897 1105 86-87 -0.07607382951630015 1105 88-89 -0.21075899511284746 1105 90-91 -0.3278699334193611 1105 92-93 -0.45921511225357037 1105 94-95 -0.37351341090955614 1105 96-97 -0.1928677308148785 1105 98-99 -0.552581291030215 1105 100-101 -1.3411742716553228 1106 1 0.1212191965732643 1106 2 0.16925011966550585 1106 3 0.20959610114199023 1106 4 0.17356110848521666 1106 5 0.17247041095671278 1106 6 0.15975367231899895 1106 7 0.12625894123581105 1106 8 0.19550972270554468 1106 9 0.21704751112809362 1106 10-11 0.21350625492420505 1106 12-13 0.16150463967557016 1106 14-15 0.2859649383937679 1106 16-17 0.08612933773687104 1106 18-19 0.3022831368777972 1106 20-21 0.29224317781397247 1106 22-23 0.38913771536763164 1106 24-25 0.32926159638871866 1106 26-27 0.2751115290613626 1106 28-29 0.2556030328171275 1106 30-31 0.3475445009054141 1106 32-33 0.28062792843400786 1106 34-35 0.30156665219156054 1106 36-37 0.1929951868316735 1106 38-39 0.33489353427482627 1106 40-41 0.30682704768958047 1106 42-43 0.20525380142394312 1106 44-45 0.19209545621223612 1106 46-47 0.18872602604473343 1106 48-49 0.023139130300890542 1106 50-51 0.04084974995149793 1106 52-53 0.03472149270864122 1106 54-55 -0.14638603592575805 1106 56-57 -0.016526269448803532 1106 58-59 0.07242522692413189 1106 60-61 -0.12572033173114505 1106 62-63 0.07915288587348357 1106 64-65 0.036134653983083354 1106 66-67 -0.35970585844150804 1106 68-69 -0.5193410325998471 1106 70-71 -0.7003682954129786 1106 72-73 -0.5119926373633952 1106 74-75 -0.3535738506467858 1106 76-77 -0.34462391052205277 1106 78-79 -0.29799253123344016 1106 80-81 -0.268941590750547 1106 82-83 -0.535801820997019 1106 84-85 -0.3749072438996883 1106 86-87 -0.41128841372484715 1106 88-89 -0.6019108513049467 1106 90-91 -1.0758877104109779 1106 92-93 -0.9638489592918411 1106 94-95 -1.0866482639840491 1106 96-97 -0.9653283185169137 1106 98-99 -1.2577391613289315 1106 100-101 -1.7528614275577716 1107 1 -0.04208768849441924 1107 2 -0.05190119118680059 1107 3 0.056120101320473736 1107 4 0.022309075173382098 1107 5 0.04352294646108135 1107 6 0.009335246153064247 1107 7 0.05170789243226892 1107 8 0.04801602039457009 1107 9 0.1034690445595956 1107 10-11 0.04417802870229792 1107 12-13 0.021081386295506377 1107 14-15 0.04870280038412034 1107 16-17 -0.03317891030891218 1107 18-19 0.01308230448759673 1107 20-21 -0.010800908037424506 1107 22-23 -0.08466095735131063 1107 24-25 0.004026523230059809 1107 26-27 0.07165307352649108 1107 28-29 0.013980683221362256 1107 30-31 -0.14004012745994743 1107 32-33 -0.10037414442852821 1107 34-35 -0.09926502812517413 1107 36-37 -0.05323380565977942 1107 38-39 -0.09451657204557762 1107 40-41 -0.1329509207428714 1107 42-43 -0.025057553887414485 1107 44-45 -0.0799723154402514 1107 46-47 -0.043336715569495254 1107 48-49 -0.03139926227736822 1107 50-51 -0.06879798511151591 1107 52-53 -0.20435389296142858 1107 54-55 -0.06868107200816809 1107 56-57 -0.09744447453644511 1107 58-59 -0.09336676071171013 1107 60-61 -0.19738570032342295 1107 62-63 -0.11518862532849994 1107 64-65 -0.22948838115821957 1107 66-67 -0.23303712296878842 1107 68-69 -0.1627087851868687 1107 70-71 -0.45241174845310894 1107 72-73 -0.5780813566913174 1107 74-75 -0.5083905179219244 1107 76-77 -0.6942556833265847 1107 78-79 -0.5995428174182784 1107 80-81 -0.42896272502417077 1107 82-83 -0.5046561486553358 1107 84-85 -0.5861602304849001 1107 86-87 -0.7049316014710278 1107 88-89 -0.6209793612372465 1107 90-91 -1.0194792794178333 1107 92-93 -0.8375660967132994 1107 94-95 -1.292129890146736 1107 96-97 -1.3633630541762365 1107 98-99 -1.3407104536025471 1107 100-101 -1.9234339797748419 1108 1 -0.04653501876943977 1108 2 -0.04607645729686283 1108 3 -0.032172216731225944 1108 4 0.03275445502497831 1108 5 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58-59 -0.36375815307018655 2316 60-61 -0.32462655480014746 2316 62-63 -0.046224555676062096 2316 64-65 0.2338932963580902 2316 66-67 -0.008889951160995224 2316 68-69 0.02611895463631697 2316 70-71 0.20809170579818925 2316 72-73 0.52067323428086 2316 74-75 0.808304902223913 2316 76-77 0.5232722458861865 2316 78-79 0.46628060554923323 2316 80-81 0.5655871488438997 2316 82-83 0.6537057464673097 2316 84-85 0.7513364149425286 2316 86-87 1.1309433477311615 2316 88-89 1.3504469646815238 2316 90-91 1.2392341749101412 2316 92-93 1.5140316382793024 2316 94-95 1.8236613289241035 2316 96-97 2.069991173351962 2316 98-99 2.049584672107663 2316 100-101 3.018610699297991 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 3.0 4 5.0 5 28.0 6 47.0 7 138.0 8 341.0 9 627.0 10 1099.0 11 1890.0 12 2791.0 13 4239.0 14 5924.0 15 7950.0 16 10454.0 17 14377.0 18 15093.0 19 12202.0 20 11455.0 21 12187.0 22 12816.0 23 14004.0 24 15829.0 25 17671.0 26 20396.0 27 23884.0 28 28938.0 29 34453.0 30 41668.0 31 50515.0 32 62735.0 33 80485.0 34 108527.0 35 154417.0 36 223125.0 37 280326.0 38 160158.0 39 9678.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.451238283156556 18.33683829037707 14.87585231552806 23.33607111093831 2 16.121647977104566 19.271367442738843 39.65265256160752 24.95433201854908 3 19.604716134891124 22.93839198184863 29.576306018334662 27.88058586492559 4 13.480733478748027 16.30951670209472 35.52036199095023 34.689387828207025 5 14.226794819728353 36.59707108468446 33.84330754305033 15.332826552536854 6 33.71527469231795 35.08554283111921 16.475013329186474 14.724169147376372 7 29.685840394646778 32.14731498849687 19.55170436480113 18.61514025205522 8 27.071846984126104 34.79345050740101 18.739473980164917 19.395228528307964 9 26.38318669219523 16.689460026463436 19.575029955334273 37.35232332600707 10-11 25.712714807168176 26.00872765845782 26.27488930757707 22.00366822679694 12-13 26.421276170350428 23.194716154355604 27.073113839549144 23.31089383574482 14-15 23.062830532642636 24.83231260734284 25.220065839256133 26.884791020758385 16-17 22.790685242678542 27.96648764530444 26.273965165812292 22.96886194620473 18-19 22.729642521440798 26.120044873993216 28.613349179924995 22.536963424640987 20-21 23.023329755817166 25.374493744437608 29.197807949861087 22.404368549884136 22-23 22.97931658796594 25.155087408485237 29.08944114384253 22.776154859706292 24-25 22.89432313258474 25.538353558147598 28.966245231036353 22.60107807823131 26-27 22.412380468322542 25.865710690985445 28.776791343448565 22.945117497243448 28-29 22.550463938460897 26.178729992328442 28.720758686045745 22.550047383164916 30-31 22.62943101345814 25.75592904755144 28.702237770054 22.912402168936417 32-33 22.337203345000756 25.771445311903413 28.77669773505739 23.114653608038445 34-35 23.052145514227753 25.96358256373086 28.42853215251251 22.55573976952888 36-37 23.165759148164845 25.508472428897168 28.405520017299953 22.920248405638034 38-39 22.6228129976771 25.855119470273706 28.449496751691495 23.072570780357697 40-41 23.060902453467698 25.647731161976356 28.529953465963093 22.761412918592857 42-43 22.76821355726522 25.779728110477507 28.23832056638328 23.21373776587399 44-45 22.84148039747917 25.95301698463982 28.387903182138153 22.817599435742856 46-47 23.24826316273597 26.1914538755378 27.72799297455184 22.83228998717439 48-49 23.07062439581457 26.144789296567623 27.684457703982545 23.100128603635266 50-51 23.102914427736355 26.413789033754043 27.602782521764485 22.880514016745117 52-53 23.366647956047053 26.208857031079415 27.50041048808335 22.924084524790185 54-55 22.96817739540835 26.440778810803156 27.540110200430373 23.05093359335812 56-57 23.01598736036533 26.595724367922795 27.37306467744942 23.015223594262448 58-59 23.03710547247825 26.223045299208508 27.333609003943753 23.40624022436949 60-61 22.77740944512904 26.426228370099103 27.642223649270182 23.154138535501676 62-63 22.788695016383382 26.527049762564236 27.767485847245375 22.916769373807007 64-65 22.53487288556333 26.42617933158769 27.621325145348536 23.417622637500443 66-67 22.549171742924223 26.574872238694674 27.40936072655141 23.466595291829698 68-69 22.900367635314655 26.227731119280783 27.687300262358562 23.184600983045996 70-71 22.651497346086373 26.39926289627744 27.341472534103023 23.60776722353316 72-73 22.785925759048933 26.517967695879875 27.11790333895791 23.578203206113283 74-75 22.70885241872238 26.931223305109903 26.897478776878884 23.46244549928883 76-77 22.846465483211432 26.390061114673596 27.262497990676742 23.500975411438233 78-79 22.872363141318647 26.55907844616602 27.216850063832837 23.351708348682497 80-81 22.942349223409124 26.743497802369852 27.021634012045393 23.292518962175627 82-83 22.80720699824266 26.739464909304868 27.025236438613113 23.428091653839356 84-85 22.946980746310057 26.643639220631236 26.940757850399834 23.46862218265887 86-87 22.96449047505292 26.69637946455853 26.86856600796662 23.47056405242193 88-89 22.75154575858565 26.873719100885634 26.60083095482035 23.773904185708368 90-91 22.857956311075696 26.77999468774901 26.719476691196764 23.642572309978522 92-93 22.9248575463955 26.372387540465436 26.90644099997361 23.79631391316545 94-95 22.670913110374208 26.341559602961677 26.954954091749844 24.032573194914267 96-97 22.92865092827183 26.047052713508766 26.915821273564372 24.10847508465503 98-99 22.649354274093827 26.274250737858402 26.60876043259921 24.46763455544856 100-101 23.199074655859878 25.58866758914109 26.552995878025087 24.659261876973943 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 551.0 1 366.5 2 269.5 3 357.0 4 278.0 5 255.5 6 486.0 7 1090.5 8 1794.0 9 2077.0 10 1764.0 11 1335.5 12 1244.0 13 1330.0 14 1503.5 15 1655.0 16 1707.0 17 1780.5 18 1931.0 19 2128.0 20 2446.5 21 2860.5 22 3398.5 23 4119.0 24 5045.5 25 6176.0 26 7492.0 27 9221.0 28 11009.5 29 12620.0 30 14340.0 31 16887.0 32 20653.0 33 22530.0 34 22830.0 35 24190.0 36 26605.0 37 29169.0 38 31365.5 39 33402.5 40 35592.5 41 38628.0 42 40499.5 43 41703.0 44 43990.5 45 47626.5 46 49604.5 47 50833.0 48 52407.0 49 52846.5 50 53687.5 51 53875.0 52 54738.0 53 56927.0 54 58301.5 55 55738.5 56 50976.5 57 47191.5 58 44038.0 59 39038.5 60 31926.5 61 26493.5 62 22254.5 63 16878.5 64 12085.0 65 8935.0 66 6464.5 67 5002.5 68 3673.0 69 2495.5 70 1875.5 71 1296.5 72 863.5 73 642.5 74 493.5 75 351.5 76 223.5 77 120.0 78 70.0 79 47.0 80 24.0 81 11.5 82 8.0 83 4.5 84 2.0 85 1.5 86 1.5 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.009510731854287257 2 0.013259487475685155 3 0.010135524457853574 4 0.0 5 9.024782051513456E-4 6 0.002082642011887721 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 8.330568047550882E-4 14-15 0.0 16-17 0.003748755621397897 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0030892523176334523 26-27 0.019021463708574514 28-29 0.006456190236851934 30-31 0.003366937919218482 32-33 0.0 34-35 0.008608253649135911 36-37 7.983461045569596E-4 38-39 0.0024991704142652647 40-41 0.0018743778106989485 42-43 0.002776856015850294 44-45 0.0 46-47 3.471070019812868E-5 48-49 3.818177021794154E-4 50-51 0.004026441222982927 52-53 0.008295857347352754 54-55 0.007115693540616379 56-57 0.01711237519767744 58-59 0.024540465040076977 60-61 0.00367933422100164 62-63 0.020756998718480947 64-65 0.022145426726406094 66-67 0.013502462377072054 68-69 0.032211529783863414 70-71 0.01711237519767744 72-73 0.005102472929124915 74-75 0.016973532396884924 76-77 0.021971873225415453 78-79 0.026970214053945982 80-81 0.012252877169939422 82-83 0.035856153304666925 84-85 0.02169418762383042 86-87 0.032628058186240955 88-89 0.022735508629774283 90-91 0.028497484862663642 92-93 0.02499170414265265 94-95 0.014856179684799074 96-97 0.015342129487572876 98-99 0.011176845463797434 100-101 0.010343788659042345 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1440478.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.16022764187314 #Duplication Level Percentage of deduplicated Percentage of total 1 80.25269573550898 47.477677485866934 2 11.50492563166651 13.612680387444238 3 3.4543086493892443 6.130730581294771 4 1.4964129147019616 3.5411251472002774 5 0.8061335149836422 2.3845521128087817 6 0.5052180316080447 1.793328825522659 7 0.34201668958290493 1.4163649649131165 8 0.24169682903720666 1.1439071540928032 9 0.19428200815099955 1.034439104604005 >10 1.0949397664539129 11.849930097173091 >50 0.06893074319883973 2.75545071547176 >100 0.033502389734406675 3.6463105935458713 >500 0.00352649713099736 1.4139440269387782 >1k 0.0011754990436657865 1.0479403178211908 >5k 2.3509980873315733E-4 0.7516184853017074 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5714 0.39667388186421454 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5094 0.353632613618535 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3033 0.21055510740184855 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 1559 0.1082279632177652 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 1538 0.10677011380944382 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 1503 0.10434036479557482 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 6.942140039625735E-5 10-11 0.0 0.0 0.0 3.471070019812868E-5 6.942140039625735E-5 12-13 0.0 0.0 0.0 6.942140039625735E-5 3.123963017831581E-4 14-15 0.0 0.0 0.0 6.942140039625735E-5 4.1652840237754415E-4 16-17 0.0 0.0 0.0 1.388428007925147E-4 4.859498027738015E-4 18-19 0.0 0.0 0.0 1.388428007925147E-4 4.859498027738015E-4 20-21 0.0 0.0 0.0 1.388428007925147E-4 5.900819033681874E-4 22-23 0.0 0.0 0.0 2.0826420118877207E-4 6.247926035663162E-4 24-25 0.0 0.0 0.0 3.818177021794154E-4 6.247926035663162E-4 26-27 0.0 0.0 0.0 0.001076031706141989 6.942140039625735E-4 28-29 0.0 0.0 0.0 0.0044776803255585996 6.942140039625735E-4 30-31 0.0 0.0 0.0 0.014092544280440242 6.942140039625735E-4 32-33 6.942140039625735E-5 0.0 0.0 0.03405119689436423 6.942140039625735E-4 34-35 6.942140039625735E-5 0.0 0.0 0.06393710976495302 6.942140039625735E-4 36-37 6.942140039625735E-5 0.0 0.0 0.11510068185699468 6.942140039625735E-4 38-39 6.942140039625735E-5 0.0 0.0 0.18538984975820527 6.942140039625735E-4 40-41 6.942140039625735E-5 0.0 0.0 0.2602261193853707 6.942140039625735E-4 42-43 6.942140039625735E-5 0.0 0.0 0.3362425528192725 6.942140039625735E-4 44-45 6.942140039625735E-5 0.0 0.0 0.4303779717565974 7.289247041607021E-4 46-47 6.942140039625735E-5 0.0 0.0 0.5414522123906091 7.636354043588308E-4 48-49 6.942140039625735E-5 0.0 0.0 0.659017353961671 8.67767504953217E-4 50-51 6.942140039625735E-5 0.0 0.0 0.7785610054440262 9.024782051513456E-4 52-53 6.942140039625735E-5 0.0 0.0 0.889669956778236 9.024782051513456E-4 54-55 6.942140039625735E-5 0.0 0.0 1.0117127786748565 9.024782051513456E-4 56-57 6.942140039625735E-5 0.0 0.0 1.1543737564891654 9.024782051513456E-4 58-59 6.942140039625735E-5 0.0 0.0 1.3027619998361655 9.024782051513456E-4 60-61 6.942140039625735E-5 0.0 0.0 1.4650692339626152 9.024782051513456E-4 62-63 6.942140039625735E-5 0.0 0.0 1.6236624231678651 9.71899605547603E-4 64-65 6.942140039625735E-5 0.0 0.0 1.7835051975802476 9.71899605547603E-4 66-67 6.942140039625735E-5 0.0 0.0 1.9627512534033844 0.0010066103057457315 68-69 6.942140039625735E-5 0.0 0.0 2.1539725007948753 0.0010413210059438602 70-71 6.942140039625735E-5 0.0 0.0 2.357064807654126 0.0010413210059438602 72-73 6.942140039625735E-5 0.0 0.0 2.5579009190004984 0.0011107424063401177 74-75 6.942140039625735E-5 0.0 0.0 2.758980005248258 0.0011107424063401177 76-77 6.942140039625735E-5 0.0 0.0 2.984530135135698 0.0011107424063401177 78-79 6.942140039625735E-5 0.0 0.0 3.231635609846176 0.001180163806736375 80-81 6.942140039625735E-5 0.0 0.0 3.490785697525405 0.0012148745069345038 82-83 6.942140039625735E-5 0.0 0.0 3.7545870190311827 0.0013190066075288898 84-85 6.942140039625735E-5 6.942140039625735E-5 0.0 4.017347019531017 0.0013190066075288898 86-87 6.942140039625735E-5 6.942140039625735E-5 0.0 4.289895437486723 0.0013537173077270183 88-89 6.942140039625735E-5 6.942140039625735E-5 0.0 4.586671924180724 0.001388428007925147 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2825 0.0 55.323196 1 GTATCAA 5050 0.0 41.671787 1 TGGTATC 1260 0.0 35.43577 2 GTGGTAT 1340 0.0 34.38719 1 ATCAACG 6360 0.0 32.709312 3 TCAACGC 6425 0.0 32.301113 4 TATCAAC 6450 0.0 32.255142 2 CAACGCA 6440 0.0 32.152134 5 AACGCAG 6665 0.0 31.137981 6 ACGCAGA 7695 0.0 26.908348 7 CGCAGAG 7785 0.0 26.536266 8 TACATGG 4940 0.0 24.51872 2 TTACACG 155 1.4134421E-6 24.511362 4 GTACATG 5095 0.0 24.148233 1 GCAGAGT 8525 0.0 24.12141 9 ACATGGG 5010 0.0 23.321247 3 TAAGGTG 825 0.0 23.025827 5 GGACCGA 490 0.0 22.291584 6 GTAAGGT 810 0.0 22.27962 4 GAGTACT 5485 0.0 22.169092 12-13 >>END_MODULE