FastQCFastQC Report
Thu 26 May 2016
SRR938083_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938083_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2165693
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99980.46165361387786724No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80980.3739218808944758No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50570.23350493352474241No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA22620.10444693684654288No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51650.050.7116171
GTATCAA89750.040.985041
ATCAACG108500.033.4683
TATCAAC110700.032.8887442
TCAACGC111450.032.6247754
CAACGCA111550.032.46775
AACGCAG116400.031.1076896
ACGCAGA131150.027.422887
CGCAGAG131850.027.2412578
GTGTAGC14300.025.356131
GCAGAGT142350.024.7284039
TGGTATC24950.024.0030352
GAGTACT89800.023.35498812-13
GTGGTAT27450.022.7684971
GTACATG80150.022.6196291
TACATGG77850.022.4675312
GTACTTT93450.022.26486614-15
CAGAGTA133650.022.16194310-11
TAGCCCT14800.021.5168764
ACCGAGT5900.020.9366328
Produced by FastQC (version 0.11.5)