##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938083_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2165693 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.685527450104885 33.0 31.0 34.0 30.0 34.0 2 31.857577689912652 33.0 31.0 34.0 30.0 34.0 3 31.935364800089395 34.0 31.0 34.0 30.0 34.0 4 35.473138159471354 37.0 35.0 37.0 33.0 37.0 5 35.478056677469986 37.0 35.0 37.0 33.0 37.0 6 35.50642773467892 37.0 35.0 37.0 33.0 37.0 7 35.42836865612993 37.0 35.0 37.0 33.0 37.0 8 35.39034849353071 37.0 35.0 37.0 33.0 37.0 9 36.99109430561026 39.0 37.0 39.0 33.0 39.0 10-11 36.8744729747014 39.0 37.0 39.0 33.0 39.0 12-13 36.72644368338449 39.0 37.0 39.0 32.5 39.0 14-15 37.82516958774859 40.0 38.0 41.0 32.5 41.0 16-17 37.63287455793596 40.0 37.5 41.0 32.0 41.0 18-19 37.828808376810564 40.0 38.0 41.0 32.0 41.0 20-21 37.81406275035289 40.0 38.0 41.0 32.0 41.0 22-23 37.69042680564604 40.0 38.0 41.0 32.0 41.0 24-25 37.541186123795015 40.0 37.5 41.0 32.0 41.0 26-27 37.31694173643264 40.0 37.0 41.0 31.5 41.0 28-29 37.0839405677536 40.0 37.0 41.0 31.0 41.0 30-31 36.80278252734806 39.5 36.0 41.0 30.0 41.0 32-33 36.44674891593591 39.0 35.5 41.0 30.0 41.0 34-35 36.125777753356545 39.0 35.0 40.5 29.5 41.0 36-37 35.7845144718111 38.5 35.0 40.0 28.0 41.0 38-39 35.42051412642512 38.0 35.0 40.0 26.5 41.0 40-41 35.21828671007387 38.0 35.0 40.0 25.5 41.0 42-43 34.91341247351309 38.0 34.0 40.0 24.5 41.0 44-45 34.50783883034207 38.0 33.5 40.0 23.5 41.0 46-47 33.99815509400455 38.0 33.0 40.0 21.5 41.0 48-49 33.461030487700704 38.0 33.0 40.0 16.5 41.0 50-51 32.62246472607151 37.5 31.5 39.5 14.5 40.5 52-53 32.58579701739812 37.0 31.5 39.5 11.0 40.5 54-55 32.52422203885777 37.0 31.5 40.0 8.5 41.0 56-57 32.01049271526481 37.0 31.0 40.0 4.5 41.0 58-59 31.49767995740855 36.0 30.0 39.5 2.0 41.0 60-61 30.894405855308207 36.0 29.0 39.0 2.0 41.0 62-63 30.231922760982282 35.0 28.0 39.0 2.0 40.0 64-65 29.575991610999345 35.0 27.5 38.5 2.0 40.0 66-67 28.846589982975427 34.0 26.0 38.0 2.0 40.0 68-69 28.104924382172356 34.0 25.5 37.5 2.0 40.0 70-71 27.231956468437584 33.5 22.5 37.0 2.0 39.0 72-73 26.390576365163483 33.0 19.0 36.0 2.0 39.0 74-75 25.523618075138074 32.0 14.5 35.5 2.0 38.5 76-77 24.659348300982643 31.5 7.5 35.0 2.0 37.5 78-79 23.793169438142897 31.0 2.0 35.0 2.0 37.0 80-81 22.967820231214674 30.0 2.0 35.0 2.0 36.0 82-83 22.122548994709774 29.5 2.0 34.0 2.0 36.0 84-85 21.294897291536707 29.0 2.0 34.0 2.0 35.0 86-87 20.512525782740212 28.0 2.0 34.0 2.0 35.0 88-89 19.66702136452397 26.5 2.0 34.0 2.0 35.0 90-91 18.797986372029648 25.5 2.0 33.5 2.0 35.0 92-93 17.89159682374187 23.0 2.0 33.0 2.0 35.0 94-95 16.95431485441381 18.5 2.0 32.5 2.0 35.0 96-97 15.867026628427944 4.0 2.0 32.0 2.0 34.0 98-99 14.585637484167885 2.0 2.0 31.5 2.0 34.0 100-101 11.768616789175567 2.0 2.0 27.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4829475034590551 1101 2 0.5064568169323813 1101 3 0.4269041408774541 1101 4 0.40085244654089536 1101 5 0.4216673804578903 1101 6 0.3998637571319179 1101 7 0.4103537222292033 1101 8 0.320907071032309 1101 9 0.6075789674747867 1101 10-11 0.5366500007363797 1101 12-13 0.5914133623598943 1101 14-15 0.6836985970275222 1101 16-17 0.7749560519370604 1101 18-19 0.819405242138906 1101 20-21 0.7714212536653946 1101 22-23 0.9677020145681823 1101 24-25 1.030784876912385 1101 26-27 1.0609897772763972 1101 28-29 1.1657145656517756 1101 30-31 1.1209193669511635 1101 32-33 1.2549534071050772 1101 34-35 1.4589407835220314 1101 36-37 1.6168140072157229 1101 38-39 1.6176425803220553 1101 40-41 1.7291744731328365 1101 42-43 1.856309628344242 1101 44-45 1.8566378065992524 1101 46-47 2.0673278887931232 1101 48-49 2.072010921108216 1101 50-51 2.295442694612163 1101 52-53 2.286312449300034 1101 54-55 2.482252428007307 1101 56-57 2.774946526975171 1101 58-59 3.1126682114658166 1101 60-61 3.217135555427472 1101 62-63 3.329600951989317 1101 64-65 3.357925740337617 1101 66-67 3.5236498691944114 1101 68-69 3.8578809140451398 1101 70-71 4.231278563450161 1101 72-73 4.422864467871442 1101 74-75 4.640232051163377 1101 76-77 4.801805582441638 1101 78-79 5.039527865405031 1101 80-81 5.064379854488056 1101 82-83 5.530831440803908 1101 84-85 5.6202362395234715 1101 86-87 5.422979554212219 1101 88-89 5.459084782646617 1101 90-91 5.4360928016424275 1101 92-93 5.593864898016221 1101 94-95 5.506560682729734 1101 96-97 5.480414683201532 1101 98-99 5.246652049668453 1101 100-101 4.298464248951166 1102 1 0.5598263369327263 1102 2 0.5987552446617705 1102 3 0.4708933231771013 1102 4 0.3365869780576247 1102 5 0.3327302160665724 1102 6 0.4026734373862766 1102 7 0.4287026310264608 1102 8 0.42607366177721673 1102 9 0.5904171989119149 1102 10-11 0.6769447842213268 1102 12-13 0.6698109185995165 1102 14-15 0.8381355336535563 1102 16-17 0.9654008959293421 1102 18-19 0.6972364910748539 1102 20-21 0.7469928524856186 1102 22-23 0.9035478404818917 1102 24-25 0.835597511213372 1102 26-27 0.9130648569942466 1102 28-29 0.859701849907438 1102 30-31 0.8808239931170263 1102 32-33 1.109984834112062 1102 34-35 1.130541214205465 1102 36-37 1.332219567449279 1102 38-39 1.4471737864657328 1102 40-41 1.617098714720477 1102 42-43 1.7038342914126758 1102 44-45 1.7247496324485425 1102 46-47 1.8676128254559714 1102 48-49 2.0470099433279927 1102 50-51 2.0914737121017737 1102 52-53 2.2453138365976386 1102 54-55 2.479812003581671 1102 56-57 2.7063221717349535 1102 58-59 2.817944256080203 1102 60-61 2.9317077018046227 1102 62-63 3.1291341482033452 1102 64-65 3.060329699312259 1102 66-67 3.3893592314976466 1102 68-69 3.683225495302125 1102 70-71 3.816416188893612 1102 72-73 3.9782646330587816 1102 74-75 4.047847061125228 1102 76-77 4.1907027153599365 1102 78-79 4.490978201071236 1102 80-81 4.615996010178279 1102 82-83 4.771233089916546 1102 84-85 4.973414197826319 1102 86-87 5.219995541494178 1102 88-89 5.395265642080929 1102 90-91 5.728841640959775 1102 92-93 5.598214869718113 1102 94-95 5.477957735124949 1102 96-97 5.480585794742103 1102 98-99 5.445048547604765 1102 100-101 4.217151182126216 1103 1 0.5520256389069935 1103 2 0.3600656056556737 1103 3 0.34929880320002127 1103 4 0.24597386142482236 1103 5 0.3283219751955997 1103 6 0.25412158032527543 1103 7 0.3144660315577994 1103 8 0.33432755561479155 1103 9 0.3326946380951199 1103 10-11 0.3255943127934984 1103 12-13 0.3550416838232451 1103 14-15 0.5171511350496161 1103 16-17 0.6591421054933377 1103 18-19 0.6831587705976929 1103 20-21 0.685384918954334 1103 22-23 0.6960805278809659 1103 24-25 0.7981893224338776 1103 26-27 0.9173200791643694 1103 28-29 0.8400308075570777 1103 30-31 0.7620293897935895 1103 32-33 0.9532090358102394 1103 34-35 0.9627903937647702 1103 36-37 1.0854850516395231 1103 38-39 1.2598919195073535 1103 40-41 1.2786441384846015 1103 42-43 1.172931025277343 1103 44-45 1.2825255870247716 1103 46-47 1.6759617862310918 1103 48-49 1.7486071981073792 1103 50-51 1.7610542455184515 1103 52-53 1.876053665737608 1103 54-55 1.993287416406595 1103 56-57 2.3068619696200443 1103 58-59 2.543998457257473 1103 60-61 2.69276086115244 1103 62-63 2.650950708082501 1103 64-65 2.7203840046724785 1103 66-67 3.082377671878959 1103 68-69 3.2692447951927335 1103 70-71 3.4927555908835117 1103 72-73 3.9125121717367044 1103 74-75 4.069552926625047 1103 76-77 4.389780045151046 1103 78-79 4.375204903160103 1103 80-81 4.669633948397792 1103 82-83 4.901103642607069 1103 84-85 5.0004366492510215 1103 86-87 4.947999917177874 1103 88-89 5.011146378654352 1103 90-91 5.156454028311963 1103 92-93 5.141108941708524 1103 94-95 5.158073768880225 1103 96-97 5.019224644562383 1103 98-99 4.8885325555226675 1103 100-101 4.049263621569876 1104 1 0.638944455227179 1104 2 0.531352697916919 1104 3 0.41659359412525276 1104 4 0.38328988299089417 1104 5 0.44225974773731025 1104 6 0.48506411442111386 1104 7 0.48750802650171465 1104 8 0.6027519884417032 1104 9 0.6375772764215384 1104 10-11 0.7384617583260606 1104 12-13 0.7057854721222583 1104 14-15 1.0305097692526246 1104 16-17 1.0812621629265209 1104 18-19 0.8971766182443233 1104 20-21 0.9638289666025983 1104 22-23 0.9235454707341333 1104 24-25 1.0094740830062463 1104 26-27 1.076558578211241 1104 28-29 1.0705627394226696 1104 30-31 1.1467217500354323 1104 32-33 1.1944202050090453 1104 34-35 1.2833899446078618 1104 36-37 1.328557992594618 1104 38-39 1.4712293476136153 1104 40-41 1.7512017966508324 1104 42-43 1.75696539928024 1104 44-45 1.7999723467953714 1104 46-47 1.927854749439497 1104 48-49 2.0677499547363283 1104 50-51 2.1762058257490082 1104 52-53 2.232229368721349 1104 54-55 2.3873331215993048 1104 56-57 2.519175536776771 1104 58-59 2.8066591188025996 1104 60-61 2.950289250487206 1104 62-63 3.0619717219171854 1104 64-65 3.213068330814391 1104 66-67 3.241852919160703 1104 68-69 3.449251176657036 1104 70-71 3.6786101139508673 1104 72-73 3.8553413080300523 1104 74-75 4.172275071124307 1104 76-77 4.553802597082257 1104 78-79 4.637729549520625 1104 80-81 4.804133535238883 1104 82-83 4.928314860894055 1104 84-85 5.0147668204869795 1104 86-87 5.221609801084316 1104 88-89 5.235449224132921 1104 90-91 5.267156582533957 1104 92-93 5.199345428139729 1104 94-95 5.244182118780824 1104 96-97 5.273051989136915 1104 98-99 5.307596361984308 1104 100-101 4.003129929892779 1105 1 0.6533192391587583 1105 2 0.5572206272140043 1105 3 0.6473868666382501 1105 4 0.4845021418294877 1105 5 0.4937503656469957 1105 6 0.45601798205796484 1105 7 0.44885683574077717 1105 8 0.5420051688236072 1105 9 0.6459965610062852 1105 10-11 0.6551882624285739 1105 12-13 0.7131003262996742 1105 14-15 0.8276376207505507 1105 16-17 0.8057196072522856 1105 18-19 0.7548388523409599 1105 20-21 0.7663676071412553 1105 22-23 0.7836452229272552 1105 24-25 0.7459697461438353 1105 26-27 0.776437846198057 1105 28-29 0.9134061901648778 1105 30-31 0.9512081535820016 1105 32-33 0.9266735432358857 1105 34-35 1.2217553682375453 1105 36-37 1.247162657832611 1105 38-39 1.2209710338817814 1105 40-41 1.3810564969923789 1105 42-43 1.56908307316872 1105 44-45 1.6197882298700321 1105 46-47 1.56853393298411 1105 48-49 1.7211513921905137 1105 50-51 1.9409707433064938 1105 52-53 1.8354293359903124 1105 54-55 2.0022010257186693 1105 56-57 2.228637590562194 1105 58-59 2.4515196197036744 1105 60-61 2.615026956738742 1105 62-63 2.8476262797140954 1105 64-65 2.9800442880277593 1105 66-67 3.0797896194654477 1105 68-69 3.1476604530977568 1105 70-71 3.331208527367103 1105 72-73 3.588051355586895 1105 74-75 3.8525769665224985 1105 76-77 3.866882834605004 1105 78-79 4.16642331483909 1105 80-81 4.49658973326747 1105 82-83 4.743616305998394 1105 84-85 4.685121337321675 1105 86-87 4.8485854048412165 1105 88-89 4.79523142232226 1105 90-91 4.727651528106506 1105 92-93 4.725499269824162 1105 94-95 4.6400818058992215 1105 96-97 4.591377786907524 1105 98-99 4.3830495125403015 1105 100-101 3.776646163228275 1106 1 0.47055517332774954 1106 2 0.37219293374858253 1106 3 0.3780552258644576 1106 4 0.2338366977049091 1106 5 0.40415662650295303 1106 6 0.33246726720612685 1106 7 0.2944721689890457 1106 8 0.26078657252420356 1106 9 0.37939026084767846 1106 10-11 0.34427905340833576 1106 12-13 0.4478678725571257 1106 14-15 0.531364107848205 1106 16-17 0.5488144020728996 1106 18-19 0.4727309404943796 1106 20-21 0.538817847846687 1106 22-23 0.5037905775312126 1106 24-25 0.5351531824060274 1106 26-27 0.5404881936353334 1106 28-29 0.5636619833472665 1106 30-31 0.6771553054904658 1106 32-33 0.775662198554457 1106 34-35 0.7787338467780174 1106 36-37 0.7963112246363977 1106 38-39 0.8544904882269151 1106 40-41 0.8915836926553098 1106 42-43 0.9058450658315991 1106 44-45 1.241818877553463 1106 46-47 1.3327613518898076 1106 48-49 1.683436333595111 1106 50-51 1.8639826067456227 1106 52-53 1.816348459150099 1106 54-55 1.9633649801611242 1106 56-57 1.8118240434423285 1106 58-59 2.2540707294446776 1106 60-61 2.68243984595836 1106 62-63 2.328013140708453 1106 64-65 2.7297303092306997 1106 66-67 2.796990069490082 1106 68-69 2.68535083112031 1106 70-71 3.0144279032000725 1106 72-73 3.304255851362793 1106 74-75 3.350990541210102 1106 76-77 3.368156910969489 1106 78-79 3.7554738485448347 1106 80-81 3.7890296081323065 1106 82-83 3.8262023923021644 1106 84-85 3.856422906912975 1106 86-87 3.764840379373659 1106 88-89 3.9980713878998415 1106 90-91 4.250935553070207 1106 92-93 4.233628258879957 1106 94-95 4.124254457216747 1106 96-97 4.196550671991984 1106 98-99 3.9451287204149867 1106 100-101 3.331603163150973 1107 1 0.5029976837487702 1107 2 0.4306275469072709 1107 3 0.3255298234923458 1107 4 0.33054885787721133 1107 5 0.414014468455143 1107 6 0.5256910425077663 1107 7 0.40652648281365344 1107 8 0.4062375882528002 1107 9 0.5113699609275315 1107 10-11 0.4905936032717122 1107 12-13 0.46380250403042567 1107 14-15 0.6623813748679765 1107 16-17 0.7558854005124971 1107 18-19 0.7178455167307973 1107 20-21 0.6502192456181675 1107 22-23 0.6989186150102356 1107 24-25 0.7939854058736415 1107 26-27 0.9566896690607862 1107 28-29 0.959097431652431 1107 30-31 1.021510374609825 1107 32-33 0.9715324358386184 1107 34-35 1.050593714326716 1107 36-37 1.0689975392228916 1107 38-39 1.056223691892768 1107 40-41 1.3106701779340213 1107 42-43 1.290374618295651 1107 44-45 1.3960934173071635 1107 46-47 1.742958380526538 1107 48-49 1.7786288385217404 1107 50-51 1.7103234380826677 1107 52-53 1.928088219448405 1107 54-55 2.070226184676777 1107 56-57 2.25459682132999 1107 58-59 2.3798683309625943 1107 60-61 2.4118079733987834 1107 62-63 2.5693379650712878 1107 64-65 2.843175618125013 1107 66-67 2.9944413159828294 1107 68-69 3.131446796044159 1107 70-71 3.2595269120130688 1107 72-73 3.5082976123497325 1107 74-75 3.656460475936811 1107 76-77 3.785302962704616 1107 78-79 3.9036922027201975 1107 80-81 3.8598123931260453 1107 82-83 4.230224190357887 1107 84-85 4.242631909645514 1107 86-87 4.2056095382932455 1107 88-89 4.434580109319885 1107 90-91 4.6335896888767465 1107 92-93 4.559442984563642 1107 94-95 4.220813232481756 1107 96-97 4.125440055375497 1107 98-99 3.9024391824561153 1107 100-101 3.055995209479626 1108 1 0.39067278856702004 1108 2 0.2809162205141469 1108 3 0.3090854016299289 1108 4 0.2550006791178703 1108 5 0.3231961333528872 1108 6 0.26386088194954027 1108 7 0.3035938299521277 1108 8 0.31178072369228005 1108 9 0.41771569932225106 1108 10-11 0.4725044569476893 1108 12-13 0.5192338584252667 1108 14-15 0.6813625113462365 1108 16-17 0.7541677549148673 1108 18-19 0.6955645812326097 1108 20-21 0.6701958732179207 1108 22-23 0.677898039017947 1108 24-25 0.6978170116817424 1108 26-27 0.7302728349107284 1108 28-29 0.8072125849467753 1108 30-31 0.8715007772474053 1108 32-33 0.8995221083239215 1108 34-35 1.0127749313475434 1108 36-37 1.1487996077367697 1108 38-39 1.3240705518616025 1108 40-41 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2311 48-49 1.424757579363515 2311 50-51 1.3677439681349028 2311 52-53 1.4322088285917118 2311 54-55 1.7099373601973795 2311 56-57 1.8746894454958607 2311 58-59 1.9602505492065845 2311 60-61 2.276288446903269 2311 62-63 2.3762838303133655 2311 64-65 2.650597154575575 2311 66-67 2.492839070648966 2311 68-69 2.6440235191164945 2311 70-71 3.250073279743912 2311 72-73 3.4960429367415102 2311 74-75 3.658354576785026 2311 76-77 3.5395804515868754 2311 78-79 3.8940426187771067 2311 80-81 4.011563348612455 2311 82-83 4.150608314838703 2311 84-85 4.429664230423732 2311 86-87 4.429219110206919 2311 88-89 4.589993466712059 2311 90-91 4.791992937293742 2311 92-93 4.900461353109076 2311 94-95 4.830483121991062 2311 96-97 4.704735067428599 2311 98-99 4.791026743846414 2311 100-101 4.247162643377017 2312 1 -0.36637800672910004 2312 2 -0.1812886320175835 2312 3 -0.12546387699892136 2312 4 -0.1052278856528659 2312 5 -0.0977718418661766 2312 6 -0.11848249955555445 2312 7 -0.08918844091058276 2312 8 -3.287093754877901E-4 2312 9 -0.1191256552725477 2312 10-11 -0.03472783621304387 2312 12-13 0.03740333142858532 2312 14-15 -0.11138751778181444 2312 16-17 -0.04925087337509382 2312 18-19 0.06571355612623364 2312 20-21 0.03442522545033455 2312 22-23 0.03269120979668827 2312 24-25 0.17994725623680097 2312 26-27 0.3102607618375046 2312 28-29 0.30371993124108343 2312 30-31 0.2504963417489492 2312 32-33 0.26902614808270187 2312 34-35 0.39364985752300896 2312 36-37 0.39423905956701333 2312 38-39 0.5740935529744959 2312 40-41 0.7540518741002487 2312 42-43 0.7926149986167346 2312 44-45 1.0504886762216756 2312 46-47 0.8208530988115612 2312 48-49 1.032008066596859 2312 50-51 1.0293856222257745 2312 52-53 1.2161588186069068 2312 54-55 1.5866219489623248 2312 56-57 1.8430229431361624 2312 58-59 1.9749926757431666 2312 60-61 2.215109632896233 2312 62-63 2.1739906106298967 2312 64-65 2.430041847586967 2312 66-67 2.442316700887517 2312 68-69 2.6306191040619282 2312 70-71 2.9226941780732894 2312 72-73 3.068791994504256 2312 74-75 2.790163141475208 2312 76-77 2.82839847783049 2312 78-79 3.570257795179593 2312 80-81 3.7848432442648914 2312 82-83 4.073602948320421 2312 84-85 4.489628184003223 2312 86-87 4.226590957093048 2312 88-89 4.499261137926233 2312 90-91 4.668513471872771 2312 92-93 4.855631840544646 2312 94-95 4.838058431456911 2312 96-97 4.717212791195726 2312 98-99 4.441388598341218 2312 100-101 4.099977474482094 2313 1 -0.29717477554810756 2313 2 -0.17639960064326132 2313 3 -0.15479320778694472 2313 4 -0.14836696924485437 2313 5 -0.20541359463128117 2313 6 -0.08339173795473442 2313 7 -0.004490890526597013 2313 8 -0.057557335346366756 2313 9 -0.07260383800040415 2313 10-11 -0.11910916608786692 2313 12-13 -0.1337666844553027 2313 14-15 0.011292924635448287 2313 16-17 0.18132803845718115 2313 18-19 -0.005086564338718347 2313 20-21 0.03990103773873699 2313 22-23 -0.04118829610269614 2313 24-25 0.11990276192245375 2313 26-27 0.09339323928674759 2313 28-29 0.11231204564393238 2313 30-31 0.21616868678389523 2313 32-33 0.333258985469584 2313 34-35 0.4811054725014685 2313 36-37 0.46395208095440665 2313 38-39 0.49433458003539243 2313 40-41 0.7963916629951271 2313 42-43 0.8756981728683186 2313 44-45 1.1275716756047771 2313 46-47 1.1012425697858248 2313 48-49 1.4048648347358252 2313 50-51 1.28863713653827 2313 52-53 1.429306014153589 2313 54-55 1.6362896988420985 2313 56-57 1.9239430913857802 2313 58-59 1.9912263000382353 2313 60-61 2.1625900694845477 2313 62-63 2.1958253696271264 2313 64-65 2.567791338768256 2313 66-67 2.694086272799751 2313 68-69 2.915101954806822 2313 70-71 3.278307456420201 2313 72-73 3.5933481442888286 2313 74-75 3.7110052789509815 2313 76-77 4.110495102382995 2313 78-79 4.246538099645054 2313 80-81 4.48877292770436 2313 82-83 4.8922830312858 2313 84-85 4.944258555744476 2313 86-87 4.9630306347521405 2313 88-89 4.942082543771701 2313 90-91 4.981624722231011 2313 92-93 5.086191808291126 2313 94-95 5.103194513139922 2313 96-97 4.9997842322577615 2313 98-99 4.818636022674168 2313 100-101 4.412533760275487 2314 1 -0.36156214650450735 2314 2 -0.26632881807773856 2314 3 -0.2664498728016831 2314 4 -0.11657115134301677 2314 5 -0.1063688432313441 2314 6 -0.16860028560372342 2314 7 -0.24078361968748396 2314 8 -0.1963636532269888 2314 9 -0.13935062780753782 2314 10-11 -0.17128157496382101 2314 12-13 -0.0581605470960227 2314 14-15 -0.12864272036041058 2314 16-17 -0.12347509037588367 2314 18-19 -0.1264704503659928 2314 20-21 -0.26649119495792206 2314 22-23 -0.18716569612510625 2314 24-25 -0.12328135122216821 2314 26-27 -0.22521456618500935 2314 28-29 -0.07491083256613962 2314 30-31 -0.011497369493447707 2314 32-33 0.1920230951882118 2314 34-35 0.17081395434811242 2314 36-37 0.3318992063993349 2314 38-39 0.30949593014695154 2314 40-41 0.37472646046892777 2314 42-43 0.6464688145771191 2314 44-45 0.839992350184211 2314 46-47 0.8671626927553078 2314 48-49 1.0577385806361548 2314 50-51 1.0990397247844115 2314 52-53 1.3203860246208947 2314 54-55 1.4395292966508109 2314 56-57 1.6999565468213973 2314 58-59 1.896583130459618 2314 60-61 1.9384419961804191 2314 62-63 2.0814355406246854 2314 64-65 2.415395919466814 2314 66-67 2.681498405287517 2314 68-69 2.9242984058158257 2314 70-71 3.335616494487546 2314 72-73 3.5074338626138477 2314 74-75 3.682032473489908 2314 76-77 3.9367088809371715 2314 78-79 4.153040211579889 2314 80-81 4.531941101718143 2314 82-83 4.7995098851181694 2314 84-85 5.05608514655065 2314 86-87 5.12173124749264 2314 88-89 5.145580511227173 2314 90-91 4.934178937413552 2314 92-93 5.357290545710434 2314 94-95 5.468645500465261 2314 96-97 5.12741865455129 2314 98-99 4.908830357931494 2314 100-101 4.615460957226652 2315 1 -0.4061273396135334 2315 2 -0.24119342776799257 2315 3 -0.22693168711413847 2315 4 -0.1931273756523666 2315 5 -0.30337467741907176 2315 6 -0.2220355134215879 2315 7 -0.21731562306430163 2315 8 -0.2888814426788926 2315 9 -0.38444001010321216 2315 10-11 -0.2788671947845458 2315 12-13 -0.18022317114012054 2315 14-15 -0.22683677647302147 2315 16-17 -0.2543275760784738 2315 18-19 -0.32692541589231183 2315 20-21 -0.27944197179142094 2315 22-23 -0.15354041335159963 2315 24-25 -0.23570954086030582 2315 26-27 -0.18447923454635884 2315 28-29 -0.13412983682714952 2315 30-31 -0.1042014455471687 2315 32-33 -0.03929526133921257 2315 34-35 0.09253999855248907 2315 36-37 0.285990986097012 2315 38-39 0.3616865283099244 2315 40-41 0.4880039259482203 2315 42-43 0.48534398447637983 2315 44-45 0.6497240651438219 2315 46-47 0.7834642327833379 2315 48-49 0.8092597375031403 2315 50-51 0.8177805390545387 2315 52-53 1.1320579127013772 2315 54-55 1.2305967107958296 2315 56-57 1.4832144738858233 2315 58-59 1.7790337127026135 2315 60-61 2.063938907423637 2315 62-63 2.1434212867234628 2315 64-65 2.375417785913065 2315 66-67 2.604344888078895 2315 68-69 2.8564126286766225 2315 70-71 3.223469388183517 2315 72-73 3.437177652766511 2315 74-75 3.689510833380041 2315 76-77 3.7528264418187156 2315 78-79 4.129585956808601 2315 80-81 4.32612468286532 2315 82-83 4.538866490142951 2315 84-85 4.715689334185278 2315 86-87 4.834063508716568 2315 88-89 4.975313013954967 2315 90-91 4.970415686447065 2315 92-93 5.333265232484397 2315 94-95 5.334331390831444 2315 96-97 5.407565411650614 2315 98-99 5.458678815434338 2315 100-101 5.215342174716518 2316 1 -0.7994025867042396 2316 2 -0.6930549889874129 2316 3 -0.5707550934494137 2316 4 -0.6890689283853675 2316 5 -0.5470001943403275 2316 6 -0.6083903452873756 2316 7 -0.635884261064696 2316 8 -0.6241784321107247 2316 9 -0.6660700820267706 2316 10-11 -0.740826009874425 2316 12-13 -0.5852635050642192 2316 14-15 -0.7066312064999352 2316 16-17 -0.4901994938915095 2316 18-19 -0.6083254519947516 2316 20-21 -0.6697090975920119 2316 22-23 -0.6153960053612408 2316 24-25 -0.627747283145176 2316 26-27 -0.5965839392331063 2316 28-29 -0.6668869377545619 2316 30-31 -0.6343414860803662 2316 32-33 -0.3013582958428245 2316 34-35 -0.24841843617243597 2316 36-37 -0.22231946086990462 2316 38-39 -0.3602764274247363 2316 40-41 -0.08835324144049395 2316 42-43 0.04007881031728999 2316 44-45 0.30672212112134645 2316 46-47 0.4466482743339597 2316 48-49 0.631164832635406 2316 50-51 0.5209160152081793 2316 52-53 0.7866845484465372 2316 54-55 1.0690711249517406 2316 56-57 1.2162486727399227 2316 58-59 1.449843075424269 2316 60-61 1.407534915291155 2316 62-63 1.8612126087759613 2316 64-65 2.2918922920833005 2316 66-67 2.4242387082324974 2316 68-69 2.4662790555199585 2316 70-71 2.848264333959534 2316 72-73 3.279558500071957 2316 74-75 3.4996830759977975 2316 76-77 3.784248162826586 2316 78-79 4.023612922867624 2316 80-81 3.8395027310597314 2316 82-83 4.180716540780139 2316 84-85 4.36734770198003 2316 86-87 4.7224589927471 2316 88-89 4.95198555019806 2316 90-91 5.26427135640515 2316 92-93 5.315732839924312 2316 94-95 5.4271289399682665 2316 96-97 5.477672784166812 2316 98-99 5.283325512647421 2316 100-101 4.717409420194336 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7819.0 3 2745.0 4 2972.0 5 3190.0 6 3886.0 7 4649.0 8 5720.0 9 7695.0 10 9545.0 11 11612.0 12 14489.0 13 17810.0 14 20840.0 15 25321.0 16 30053.0 17 36082.0 18 37020.0 19 34991.0 20 36405.0 21 39904.0 22 44084.0 23 48647.0 24 53594.0 25 57895.0 26 62806.0 27 68198.0 28 73093.0 29 79024.0 30 86199.0 31 94220.0 32 105636.0 33 122509.0 34 145741.0 35 178694.0 36 216182.0 37 233877.0 38 133076.0 39 9470.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.40927609800593 18.662601471516595 14.890090484273017 23.038031946204455 2 15.235011322535936 19.505475599623306 41.16590482603753 24.09360825180322 3 19.074403774745903 23.947268492383706 29.902545477391346 27.075782255479048 4 12.57485472501686 16.591280729469805 37.27257189841375 33.561292647099584 5 13.435684524607069 37.37452515226084 34.528303281589444 14.661487041542651 6 32.15561514422944 36.24193104263958 17.18477704452087 14.417676768610116 7 28.40787055406029 33.05636400504208 20.39020203872459 18.145563402173046 8 26.20531914058296 35.23486073525356 19.381824614593018 19.177995509570454 9 26.25766474436995 16.846709731656002 20.251738496820934 36.64388702715311 10-11 24.97708472428114 26.58541491487111 27.02418268849936 21.41331767234839 12-13 25.943457121484947 23.995423618939142 27.51768509389826 22.54343416567765 14-15 22.65007566452109 25.40854569164854 26.140781502616793 25.800597141213576 16-17 21.87396798138429 28.589207746877328 27.01284910808188 22.5239751636565 18-19 21.799475923398077 26.855981468185796 29.617764012190428 21.726778596225696 20-21 22.40498912059636 26.069919655702268 30.019624730439997 21.505466493261373 22-23 22.142615325568215 26.033887674450717 29.890820648702427 21.93267635127864 24-25 21.91955987921532 26.17488001105014 29.99727211223283 21.90828799750171 26-27 21.57982284173825 26.53054908344435 29.789236545450848 22.10039152936655 28-29 21.642306704578417 26.85300143924236 29.68649828085364 21.818193575325584 30-31 21.833354735775305 26.42752748212126 29.542137096960452 22.19698068514298 32-33 21.49251139129063 26.47121385232675 29.637310174987867 22.398964581394754 34-35 22.22186296305363 26.730976110753645 29.355425924582978 21.69173500160974 36-37 22.28858286010962 26.218325882773545 29.38679999150091 22.106291265615923 38-39 21.808804682899797 26.547423596919632 29.33053929548682 22.31323242469375 40-41 22.343564087354135 26.39308714655455 29.331945762833968 21.931403003257344 42-43 21.99837353019683 26.38977272832228 29.183680255062978 22.428173486417915 44-45 22.051152039577268 26.68577092758183 29.222938689730316 22.040138343110584 46-47 22.387731256985045 26.958745508788457 28.517877100132083 22.135646134094415 48-49 22.214476639795475 26.95461145141388 28.480917081064305 22.34999482772634 50-51 22.358049645046187 27.248575728483075 28.28427522046943 22.109099406001313 52-53 22.602022569606785 27.022760084160986 28.23969119114855 22.135526155083678 54-55 22.226467909988752 27.133731314612696 28.39714794449196 22.242652830906597 56-57 22.30327252828699 27.25841625192938 28.162491586922815 22.275819632860816 58-59 22.28418269435009 26.912447567172197 28.1930229937921 22.610346744685607 60-61 22.05509097285764 27.131160088355756 28.438810498976363 22.374938439810236 62-63 22.152445846795924 27.088261791704625 28.560877072030493 22.198415289468958 64-65 21.954369747549915 27.12985278016492 28.40625673909058 22.509520733194584 66-67 21.89635734502768 27.14493805309326 28.313179992006848 22.645524609872208 68-69 22.353294842744408 26.79424312796143 28.434518512840377 22.417943516453782 70-71 22.240567012582705 26.838122978497108 28.061594775998948 22.85971523292124 72-73 22.208398679589987 26.950227857547933 28.06002896478599 22.781344498076095 74-75 22.188170034454462 27.221864844857947 27.957803097438106 22.63216202324949 76-77 22.31729789499228 26.876235409725997 28.011825299759668 22.79464139552206 78-79 22.399166037500606 26.986629355387628 27.94083761050811 22.673366996603658 80-81 22.540330578092593 27.032252686954273 27.61848885908836 22.80892787586478 82-83 22.584941309792445 27.02319415944868 27.66799331603309 22.723871214725786 84-85 22.606460103435538 27.02929903952715 27.65452992242335 22.709710934613963 86-87 22.63037998004783 26.924274806128977 27.55883315144277 22.886512062380422 88-89 22.680427131402183 26.983054824094992 27.37879404033413 22.95772400416869 90-91 22.6251081275427 26.963487643787115 27.371774474002514 23.039629754667672 92-93 22.682173184594895 26.590390189026387 27.550484619093275 23.17695200728545 94-95 22.58210448334725 26.44187425640874 27.627648293342595 23.34837296690141 96-97 22.799713662236897 26.165567883987023 27.550634445971067 23.484084007805013 98-99 22.536243546844076 26.30185933557519 27.22805188063731 23.933845236943423 100-101 23.327720915290236 25.280149027611014 27.47234333668846 23.91978672041029 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 819.0 1 536.5 2 382.5 3 522.5 4 438.0 5 390.5 6 734.0 7 1666.0 8 2850.0 9 3365.0 10 2827.0 11 2200.0 12 2106.5 13 2227.5 14 2433.5 15 2650.0 16 2834.5 17 3068.0 18 3395.5 19 3782.0 20 4341.0 21 5157.5 22 6296.0 23 7773.5 24 9535.0 25 12008.5 26 15096.5 27 18353.0 28 21129.0 29 23831.0 30 27149.0 31 31522.5 32 36660.0 33 39831.5 34 41966.0 35 44984.0 36 48758.5 37 52563.0 38 55694.0 39 58246.5 40 61236.0 41 64832.0 42 67082.5 43 69080.5 44 71326.5 45 74484.5 46 76476.0 47 77897.5 48 78988.5 49 77409.5 50 75531.5 51 73552.5 52 71910.5 53 71167.5 54 71050.0 55 68105.0 56 62874.5 57 58097.0 58 53780.5 59 48093.5 60 40160.5 61 33401.5 62 28003.0 63 22236.5 64 17065.0 65 13263.5 66 9977.0 67 7483.0 68 5693.5 69 4262.5 70 3281.5 71 2413.0 72 1666.0 73 1213.5 74 914.5 75 629.0 76 433.0 77 317.5 78 212.5 79 125.0 80 79.0 81 54.5 82 35.0 83 24.5 84 15.5 85 9.5 86 8.0 87 7.0 88 3.5 89 3.5 90 3.5 91 1.0 92 0.0 93 1.0 94 1.5 95 1.0 96 0.5 97 1.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3782161183510313 2 0.025026631198420093 3 0.0457590249402847 4 0.0384634387237711 5 0.0607195941437683 6 0.05656388047613396 7 0.03287631257061827 8 0.029736440021739 9 0.03453859803767201 10-11 0.03012892409034891 12-13 0.03063684464972644 14-15 0.025095893092880663 16-17 0.029551741636510805 18-19 0.025465289863337046 20-21 0.037978605462547096 22-23 0.024934282005805993 24-25 0.046844127953500334 26-27 0.03920223226468387 28-29 0.03070610654418701 30-31 0.038371089531157 32-33 0.03562370105088764 34-35 0.034353899652443814 36-37 0.03486182021182134 38-39 0.016992251440993714 40-41 0.012813450475205858 42-43 0.012328617213981853 44-45 0.009581228733712489 46-47 0.01629963249638799 48-49 0.013898553488421488 50-51 0.009234919261409628 52-53 0.0037632295990244233 54-55 0.004478935841783669 56-57 0.007641895688816467 58-59 0.003001348759958129 60-61 0.005979610221762734 62-63 0.005725649942073969 64-65 0.0072032370238995095 66-67 0.00408645177317376 68-69 0.006556792675600835 70-71 0.017523259298524767 72-73 0.014083251873649682 74-75 0.01043545876539288 76-77 0.007688070285123515 78-79 0.005910348327302162 80-81 0.008473038422343333 82-83 0.00981210171524773 84-85 0.004294237456555477 86-87 6.233570501451498E-4 88-89 0.023295083836905785 90-91 0.019670378026802507 92-93 0.012975061562280527 94-95 0.02424166306120027 96-97 0.01930098125634612 98-99 0.017384735509603625 100-101 0.10784076967511093 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2165693.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.35407861278327 #Duplication Level Percentage of deduplicated Percentage of total 1 76.52524947684839 43.89035174358014 2 13.396557940628936 15.36694474535076 3 4.485931147196406 7.718593430035073 4 1.9766058533548367 4.534656299992035 5 1.035356126924133 2.969094834791677 6 0.6016525953577917 2.070433815104152 7 0.40345085606976444 1.6197686480793985 8 0.26669400578319064 1.2236791178597752 9 0.20147858406635455 1.0400056694400563 >10 1.0106854059531472 10.431729692417116 >50 0.06051060821867326 2.361706355938676 >100 0.03088627114548428 3.1426208022271336 >500 0.003402088442972804 1.3985255180765797 >1k 0.0012960336925610032 1.156246988469211 >5k 2.430063173551881E-4 1.0756423386382048 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9998 0.46165361387786724 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8098 0.3739218808944758 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5057 0.23350493352474241 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2262 0.10444693684654288 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 2.3087298153524067E-5 16-17 0.0 0.0 0.0 0.0 4.6174596307048135E-5 18-19 0.0 0.0 0.0 2.3087298153524067E-5 4.6174596307048135E-5 20-21 0.0 0.0 0.0 4.6174596307048135E-5 4.6174596307048135E-5 22-23 0.0 0.0 0.0 6.92618944605722E-5 4.6174596307048135E-5 24-25 6.92618944605722E-5 0.0 0.0 4.6174596307048133E-4 4.6174596307048135E-5 26-27 9.234919261409627E-5 0.0 0.0 9.465792242944868E-4 4.6174596307048135E-5 28-29 1.1543649076762035E-4 0.0 0.0 0.0035554439156427068 4.6174596307048135E-5 30-31 1.385237889211444E-4 0.0 0.0 0.013205934543815766 4.6174596307048135E-5 32-33 1.385237889211444E-4 0.0 0.0 0.03322262204292113 4.6174596307048135E-5 34-35 2.077856833817166E-4 0.0 0.0 0.06355933181665176 4.6174596307048135E-5 36-37 2.308729815352407E-4 0.0 0.0 0.10929526945878294 4.6174596307048135E-5 38-39 3.0013487599581286E-4 0.0 0.0 0.18058884615686527 4.6174596307048135E-5 40-41 3.2322217414933695E-4 0.0 0.0 0.25398336698691826 4.6174596307048135E-5 42-43 4.386586649169573E-4 0.0 0.0 0.32846299083018693 4.6174596307048135E-5 44-45 5.540951556845776E-4 0.0 0.0 0.41672573167110943 6.92618944605722E-5 46-47 5.540951556845776E-4 0.0 0.0 0.5195334703487521 9.234919261409627E-5 48-49 5.540951556845776E-4 0.0 0.0 0.6275127638127842 9.234919261409627E-5 50-51 5.540951556845776E-4 0.0 0.0 0.7398093820315252 9.234919261409627E-5 52-53 5.540951556845776E-4 4.6174596307048135E-5 0.0 0.8480195484770925 1.1543649076762035E-4 54-55 5.540951556845776E-4 4.6174596307048135E-5 0.0 0.9677964512975754 2.308729815352407E-4 56-57 5.540951556845776E-4 4.6174596307048135E-5 0.0 1.103595939036604 2.308729815352407E-4 58-59 5.540951556845776E-4 4.6174596307048135E-5 0.0 1.2469218859736815 2.308729815352407E-4 60-61 5.540951556845776E-4 9.234919261409627E-5 0.0 1.4005447678872305 2.308729815352407E-4 62-63 5.540951556845776E-4 9.234919261409627E-5 0.0 1.5485112617531662 2.308729815352407E-4 64-65 5.540951556845776E-4 9.234919261409627E-5 0.0 1.7018570960888733 2.308729815352407E-4 66-67 6.002697519916258E-4 9.234919261409627E-5 0.0 1.8775514350371914 2.308729815352407E-4 68-69 6.002697519916258E-4 9.234919261409627E-5 0.0 2.0684833907668354 2.770475778422888E-4 70-71 6.002697519916258E-4 9.234919261409627E-5 0.0 2.2704049004175566 2.770475778422888E-4 72-73 6.002697519916258E-4 9.234919261409627E-5 0.0 2.4716799657199795 3.0013487599581286E-4 74-75 6.002697519916258E-4 9.234919261409627E-5 0.0 2.6702538171384402 3.924840686099091E-4 76-77 6.002697519916258E-4 9.234919261409627E-5 0.0 2.8827031347471683 4.155713667634332E-4 78-79 6.002697519916258E-4 9.234919261409627E-5 0.0 3.122811035543819 4.155713667634332E-4 80-81 7.387935409127702E-4 9.234919261409627E-5 0.0 3.3699374749791406 4.155713667634332E-4 82-83 7.387935409127702E-4 9.234919261409627E-5 0.0 3.6206193583301047 4.155713667634332E-4 84-85 7.618808390662942E-4 9.234919261409627E-5 0.0 3.876265010784077 4.155713667634332E-4 86-87 7.849681372198183E-4 9.234919261409627E-5 0.0 4.13525832147031 4.155713667634332E-4 88-89 7.849681372198183E-4 9.234919261409627E-5 0.0 4.403763598995795 4.155713667634332E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5165 0.0 50.711617 1 GTATCAA 8975 0.0 40.98504 1 ATCAACG 10850 0.0 33.468 3 TATCAAC 11070 0.0 32.888744 2 TCAACGC 11145 0.0 32.624775 4 CAACGCA 11155 0.0 32.4677 5 AACGCAG 11640 0.0 31.107689 6 ACGCAGA 13115 0.0 27.42288 7 CGCAGAG 13185 0.0 27.241257 8 GTGTAGC 1430 0.0 25.35613 1 GCAGAGT 14235 0.0 24.728403 9 TGGTATC 2495 0.0 24.003035 2 GAGTACT 8980 0.0 23.354988 12-13 GTGGTAT 2745 0.0 22.768497 1 GTACATG 8015 0.0 22.619629 1 TACATGG 7785 0.0 22.467531 2 GTACTTT 9345 0.0 22.264866 14-15 CAGAGTA 13365 0.0 22.161943 10-11 TAGCCCT 1480 0.0 21.516876 4 ACCGAGT 590 0.0 20.936632 8 >>END_MODULE