FastQCFastQC Report
Thu 26 May 2016
SRR938083_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938083_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2165693
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96420.44521545759255815No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84000.38786660897920433No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53490.2469879156464005No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC22230.102646127590568No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA21740.10038357237152265No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50950.052.742841
GTATCAA86100.039.1653751
ATCAACG105000.032.0651253
TATCAAC108100.031.2826232
TCAACGC107600.031.2446774
CAACGCA108400.031.0126535
AACGCAG113200.029.7053036
ACCGTCG1004.9587175E-528.4969548
CCGTCGT908.9557027E-426.3860669
ACGCAGA128350.026.198997
CGCAGAG127850.026.1528558
GTGGTAT24400.024.1708051
TGGTATC23000.023.9877432
GCAGAGT142700.023.3314239
GAGTACT93900.022.4070812-13
TACATGG70150.021.8316942
GTACATG72850.021.740721
CAGAGTA136750.021.60280810-11
GTACTTT97400.021.57751814-15
TACCGTC2005.3961776E-721.3727157
Produced by FastQC (version 0.11.5)